Bovine herpesvirus 4 (BoHV-4) (Movar virus)
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q99CW7|Q99CW7_BHV4 Tegument protein/v-FGAM-synthetase OS=Bovine herpesvirus 4 OX=10385 PE=4 SV=1
MM1 pKa = 6.94 KK2 pKa = 10.01 TKK4 pKa = 10.7 ASDD7 pKa = 3.38 QLQDD11 pKa = 3.95 DD12 pKa = 4.29 VEE14 pKa = 4.53 KK15 pKa = 10.93 QSSHH19 pKa = 6.53 PVLHH23 pKa = 6.5 LQDD26 pKa = 5.25 DD27 pKa = 4.54 EE28 pKa = 5.59 NDD30 pKa = 3.92 APPPGPPPNPPVEE43 pKa = 4.45 RR44 pKa = 11.84 PNGTHH49 pKa = 7.19 GPPQLMEE56 pKa = 4.05 YY57 pKa = 9.9 EE58 pKa = 4.5 EE59 pKa = 5.38 PGDD62 pKa = 4.6 DD63 pKa = 4.56 PPPGPPPSLPPVEE76 pKa = 4.75 RR77 pKa = 11.84 PNGTRR82 pKa = 11.84 GSNRR86 pKa = 11.84 PGDD89 pKa = 3.83 NDD91 pKa = 4.78 DD92 pKa = 5.31 PEE94 pKa = 6.89 DD95 pKa = 4.62 DD96 pKa = 4.57 PPPRR100 pKa = 11.84 PPPSPPPVNSQNSNGRR116 pKa = 11.84 PPSPHH121 pKa = 7.06 EE122 pKa = 4.01 YY123 pKa = 10.45 DD124 pKa = 4.13 EE125 pKa = 5.24 PPDD128 pKa = 4.37 DD129 pKa = 5.36 GGNHH133 pKa = 6.93 DD134 pKa = 5.4 PPPGPYY140 pKa = 9.81 DD141 pKa = 3.75 DD142 pKa = 5.84 AGLSSTPGNYY152 pKa = 9.07 EE153 pKa = 3.77 GPIEE157 pKa = 4.43 GAPEE161 pKa = 4.97 DD162 pKa = 4.14 YY163 pKa = 10.95 LKK165 pKa = 11.22 LHH167 pKa = 6.03 TRR169 pKa = 11.84 VV170 pKa = 3.18
Molecular weight: 18.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.259
IPC2_protein 4.126
IPC_protein 4.101
Toseland 3.897
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.935
Grimsley 3.808
Solomon 4.075
Lehninger 4.037
Nozaki 4.202
DTASelect 4.431
Thurlkill 3.948
EMBOSS 4.024
Sillero 4.228
Patrickios 3.617
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.134
Protein with the highest isoelectric point:
>tr|Q99CW5|Q99CW5_BHV4 Uncharacterized protein OS=Bovine herpesvirus 4 OX=10385 PE=4 SV=1
MM1 pKa = 6.53 NTRR4 pKa = 11.84 RR5 pKa = 11.84 ANKK8 pKa = 9.68 YY9 pKa = 8.56 FFRR12 pKa = 11.84 CLCGDD17 pKa = 4.01 ILRR20 pKa = 11.84 LTKK23 pKa = 10.7 NRR25 pKa = 11.84 VHH27 pKa = 6.5 NSKK30 pKa = 10.35 SACTNAVTSNNSCFTPFSAQAPAMAHH56 pKa = 6.48 AARR59 pKa = 11.84 HH60 pKa = 4.99 AEE62 pKa = 3.97 MYY64 pKa = 10.29 NIHH67 pKa = 7.19 RR68 pKa = 11.84 NTLVKK73 pKa = 10.35 DD74 pKa = 3.31 KK75 pKa = 10.85 RR76 pKa = 11.84 LQNKK80 pKa = 8.97 KK81 pKa = 10.16 RR82 pKa = 11.84 EE83 pKa = 4.22 QTVKK87 pKa = 10.59 SYY89 pKa = 11.51
Molecular weight: 10.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.706
IPC_protein 10.145
Toseland 10.643
ProMoST 10.262
Dawson 10.745
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 11.155
Grimsley 10.789
Solomon 10.818
Lehninger 10.789
Nozaki 10.657
DTASelect 10.394
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.672
Patrickios 10.906
IPC_peptide 10.818
IPC2_peptide 9.531
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
0
78
32335
57
2569
414.6
46.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.543 ± 0.176
2.598 ± 0.195
4.868 ± 0.136
4.865 ± 0.187
4.859 ± 0.186
4.676 ± 0.215
2.824 ± 0.082
7.249 ± 0.218
5.944 ± 0.195
9.964 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.629 ± 0.139
5.356 ± 0.158
5.749 ± 0.355
4.135 ± 0.164
3.776 ± 0.16
8.059 ± 0.237
7.128 ± 0.256
5.833 ± 0.247
1.03 ± 0.072
3.915 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here