Desulfonispora thiosulfatigenes DSM 11270

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfonispora; Desulfonispora thiosulfatigenes

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2407 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1ULJ1|A0A1W1ULJ1_DESTI Peptide/nickel transport system substrate-binding protein OS=Desulfonispora thiosulfatigenes DSM 11270 OX=656914 GN=SAMN00017405_2154 PE=4 SV=1
MM1 pKa = 7.37ANKK4 pKa = 10.35YY5 pKa = 10.42KK6 pKa = 10.78DD7 pKa = 4.18DD8 pKa = 4.81FDD10 pKa = 6.66DD11 pKa = 6.41FDD13 pKa = 6.52DD14 pKa = 5.86LDD16 pKa = 5.57LMDD19 pKa = 5.41DD20 pKa = 3.98TDD22 pKa = 6.53DD23 pKa = 3.91MDD25 pKa = 4.13SSQSIKK31 pKa = 10.6QEE33 pKa = 4.32TNCPVQPQQAEE44 pKa = 4.7VACDD48 pKa = 3.34PCEE51 pKa = 4.1RR52 pKa = 11.84RR53 pKa = 11.84VGTCCFTTIPAPFTGNLVADD73 pKa = 4.49SEE75 pKa = 4.58RR76 pKa = 11.84LVYY79 pKa = 10.32DD80 pKa = 3.91VSCLSCVVRR89 pKa = 11.84EE90 pKa = 4.2CTVDD94 pKa = 3.08IDD96 pKa = 4.93DD97 pKa = 5.92ALGCPPLGDD106 pKa = 3.61LDD108 pKa = 4.16VYY110 pKa = 8.64QVRR113 pKa = 11.84IVGCIPYY120 pKa = 9.65IASKK124 pKa = 10.71LLQTSACGGQATADD138 pKa = 3.64GTVPDD143 pKa = 3.79GDD145 pKa = 3.47KK146 pKa = 10.57RR147 pKa = 11.84VAISCQGSVCVDD159 pKa = 3.14NPICIRR165 pKa = 11.84LTRR168 pKa = 11.84EE169 pKa = 3.51EE170 pKa = 4.06AQAVCDD176 pKa = 4.13ALNTSLASGDD186 pKa = 3.96CTVVPARR193 pKa = 11.84VFADD197 pKa = 4.0LRR199 pKa = 11.84DD200 pKa = 3.67ICPDD204 pKa = 3.34ALDD207 pKa = 4.29PDD209 pKa = 3.87SRR211 pKa = 11.84VARR214 pKa = 11.84FRR216 pKa = 11.84VIFRR220 pKa = 11.84LPSCPTVAPP229 pKa = 4.26

Molecular weight: 24.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1V303|A0A1W1V303_DESTI Deoxyribose-phosphate aldolase OS=Desulfonispora thiosulfatigenes DSM 11270 OX=656914 GN=deoC PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.57MTYY5 pKa = 8.43QPKK8 pKa = 8.86NRR10 pKa = 11.84KK11 pKa = 8.1HH12 pKa = 5.87KK13 pKa = 9.89RR14 pKa = 11.84VHH16 pKa = 6.01GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTPTGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2407

0

2407

705465

30

1917

293.1

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.26 ± 0.051

0.992 ± 0.018

5.157 ± 0.036

7.457 ± 0.054

4.233 ± 0.044

6.72 ± 0.048

1.542 ± 0.018

9.393 ± 0.052

8.41 ± 0.054

9.965 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.023

5.608 ± 0.04

3.308 ± 0.033

2.976 ± 0.028

3.483 ± 0.031

5.857 ± 0.035

5.118 ± 0.039

6.669 ± 0.045

0.766 ± 0.015

3.545 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski