Wenling narna-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KII8|A0A1L3KII8_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 5 OX=1923505 PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 5.62AVTSQASALTHH13 pKa = 5.17VQLVSSIKK21 pKa = 10.41YY22 pKa = 9.48YY23 pKa = 9.75STLYY27 pKa = 8.01MCKK30 pKa = 8.24MTNTPFDD37 pKa = 3.79EE38 pKa = 4.6PAQPRR43 pKa = 11.84FHH45 pKa = 6.73SCRR48 pKa = 11.84LHH50 pKa = 6.24RR51 pKa = 11.84HH52 pKa = 5.81LLRR55 pKa = 11.84CSFNPSMKK63 pKa = 9.26QRR65 pKa = 11.84RR66 pKa = 11.84MLVSLLMVKK75 pKa = 10.19KK76 pKa = 10.58GYY78 pKa = 10.62GLAPEE83 pKa = 5.01DD84 pKa = 3.61MVEE87 pKa = 4.05NSLSEE92 pKa = 3.99HH93 pKa = 6.23AAVLGTPGIIADD105 pKa = 4.03GQISDD110 pKa = 5.11GILDD114 pKa = 3.82LVEE117 pKa = 4.46GLFPVGWDD125 pKa = 3.26KK126 pKa = 11.33HH127 pKa = 4.65GHH129 pKa = 5.78CGSLQSRR136 pKa = 11.84AVYY139 pKa = 10.05EE140 pKa = 4.27GGVATNHH147 pKa = 5.74VGSVGLPAFGDD158 pKa = 3.33ACFNYY163 pKa = 10.17NRR165 pKa = 11.84FLCMEE170 pKa = 4.33RR171 pKa = 11.84HH172 pKa = 5.67YY173 pKa = 10.81KK174 pKa = 10.2VKK176 pKa = 10.54ACAVQQPGKK185 pKa = 10.35IRR187 pKa = 11.84IITKK191 pKa = 10.34GEE193 pKa = 3.9VSLKK197 pKa = 10.67CLMPLQNSIMAQLRR211 pKa = 11.84KK212 pKa = 10.07NKK214 pKa = 9.95SFCLTNRR221 pKa = 11.84EE222 pKa = 4.22LEE224 pKa = 4.19KK225 pKa = 10.57TDD227 pKa = 4.03IPKK230 pKa = 10.29ADD232 pKa = 3.87PKK234 pKa = 10.94RR235 pKa = 11.84PFVSGDD241 pKa = 3.3YY242 pKa = 10.69KK243 pKa = 11.15SATDD247 pKa = 3.68YY248 pKa = 11.22LCPEE252 pKa = 4.17LQYY255 pKa = 11.45NIMRR259 pKa = 11.84VILEE263 pKa = 4.11RR264 pKa = 11.84STSEE268 pKa = 3.8WVKK271 pKa = 10.76KK272 pKa = 10.12LWWTALEE279 pKa = 4.0EE280 pKa = 4.59VSCHH284 pKa = 5.65EE285 pKa = 3.81IEE287 pKa = 4.52YY288 pKa = 10.16PNGTSIHH295 pKa = 4.93QARR298 pKa = 11.84GQLMGSLLSFPLLCIINYY316 pKa = 9.47CVLQYY321 pKa = 10.91LFKK324 pKa = 11.17GRR326 pKa = 11.84WCLINGDD333 pKa = 4.92DD334 pKa = 5.82ILFQANKK341 pKa = 9.91QEE343 pKa = 3.89YY344 pKa = 9.48SLWCEE349 pKa = 3.55TVARR353 pKa = 11.84VGFQLSLGKK362 pKa = 10.23NYY364 pKa = 7.99HH365 pKa = 4.88TNKK368 pKa = 10.39LYY370 pKa = 10.43TINSRR375 pKa = 11.84FFKK378 pKa = 11.26YY379 pKa = 10.51NFLKK383 pKa = 9.56NTHH386 pKa = 6.67DD387 pKa = 4.51EE388 pKa = 3.98ICFLHH393 pKa = 6.93LGRR396 pKa = 11.84MFGPMSGDD404 pKa = 3.34TYY406 pKa = 11.18RR407 pKa = 11.84RR408 pKa = 11.84IPSYY412 pKa = 8.85YY413 pKa = 7.57QHH415 pKa = 6.43MYY417 pKa = 10.32KK418 pKa = 10.25RR419 pKa = 11.84YY420 pKa = 8.1CKK422 pKa = 9.9KK423 pKa = 10.09VPHH426 pKa = 6.21MRR428 pKa = 11.84DD429 pKa = 3.04LLEE432 pKa = 4.14PVCYY436 pKa = 10.26GGMGGVTFDD445 pKa = 4.83EE446 pKa = 4.49YY447 pKa = 11.68LKK449 pKa = 9.85MVPVRR454 pKa = 11.84RR455 pKa = 11.84GSSLALHH462 pKa = 6.2HH463 pKa = 5.86QLVRR467 pKa = 11.84LGTPRR472 pKa = 11.84LAVMAYY478 pKa = 10.19DD479 pKa = 3.68VLVSLWFGWLTWSIPTCRR497 pKa = 11.84RR498 pKa = 11.84PEE500 pKa = 3.89HH501 pKa = 6.81PIGGFPRR508 pKa = 11.84VKK510 pKa = 10.63GKK512 pKa = 10.29LSCLLEE518 pKa = 4.06PRR520 pKa = 11.84MKK522 pKa = 10.12PWAPNVLEE530 pKa = 5.0FIRR533 pKa = 5.2

Molecular weight: 60.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KII8|A0A1L3KII8_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 5 OX=1923505 PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 5.62AVTSQASALTHH13 pKa = 5.17VQLVSSIKK21 pKa = 10.41YY22 pKa = 9.48YY23 pKa = 9.75STLYY27 pKa = 8.01MCKK30 pKa = 8.24MTNTPFDD37 pKa = 3.79EE38 pKa = 4.6PAQPRR43 pKa = 11.84FHH45 pKa = 6.73SCRR48 pKa = 11.84LHH50 pKa = 6.24RR51 pKa = 11.84HH52 pKa = 5.81LLRR55 pKa = 11.84CSFNPSMKK63 pKa = 9.26QRR65 pKa = 11.84RR66 pKa = 11.84MLVSLLMVKK75 pKa = 10.19KK76 pKa = 10.58GYY78 pKa = 10.62GLAPEE83 pKa = 5.01DD84 pKa = 3.61MVEE87 pKa = 4.05NSLSEE92 pKa = 3.99HH93 pKa = 6.23AAVLGTPGIIADD105 pKa = 4.03GQISDD110 pKa = 5.11GILDD114 pKa = 3.82LVEE117 pKa = 4.46GLFPVGWDD125 pKa = 3.26KK126 pKa = 11.33HH127 pKa = 4.65GHH129 pKa = 5.78CGSLQSRR136 pKa = 11.84AVYY139 pKa = 10.05EE140 pKa = 4.27GGVATNHH147 pKa = 5.74VGSVGLPAFGDD158 pKa = 3.33ACFNYY163 pKa = 10.17NRR165 pKa = 11.84FLCMEE170 pKa = 4.33RR171 pKa = 11.84HH172 pKa = 5.67YY173 pKa = 10.81KK174 pKa = 10.2VKK176 pKa = 10.54ACAVQQPGKK185 pKa = 10.35IRR187 pKa = 11.84IITKK191 pKa = 10.34GEE193 pKa = 3.9VSLKK197 pKa = 10.67CLMPLQNSIMAQLRR211 pKa = 11.84KK212 pKa = 10.07NKK214 pKa = 9.95SFCLTNRR221 pKa = 11.84EE222 pKa = 4.22LEE224 pKa = 4.19KK225 pKa = 10.57TDD227 pKa = 4.03IPKK230 pKa = 10.29ADD232 pKa = 3.87PKK234 pKa = 10.94RR235 pKa = 11.84PFVSGDD241 pKa = 3.3YY242 pKa = 10.69KK243 pKa = 11.15SATDD247 pKa = 3.68YY248 pKa = 11.22LCPEE252 pKa = 4.17LQYY255 pKa = 11.45NIMRR259 pKa = 11.84VILEE263 pKa = 4.11RR264 pKa = 11.84STSEE268 pKa = 3.8WVKK271 pKa = 10.76KK272 pKa = 10.12LWWTALEE279 pKa = 4.0EE280 pKa = 4.59VSCHH284 pKa = 5.65EE285 pKa = 3.81IEE287 pKa = 4.52YY288 pKa = 10.16PNGTSIHH295 pKa = 4.93QARR298 pKa = 11.84GQLMGSLLSFPLLCIINYY316 pKa = 9.47CVLQYY321 pKa = 10.91LFKK324 pKa = 11.17GRR326 pKa = 11.84WCLINGDD333 pKa = 4.92DD334 pKa = 5.82ILFQANKK341 pKa = 9.91QEE343 pKa = 3.89YY344 pKa = 9.48SLWCEE349 pKa = 3.55TVARR353 pKa = 11.84VGFQLSLGKK362 pKa = 10.23NYY364 pKa = 7.99HH365 pKa = 4.88TNKK368 pKa = 10.39LYY370 pKa = 10.43TINSRR375 pKa = 11.84FFKK378 pKa = 11.26YY379 pKa = 10.51NFLKK383 pKa = 9.56NTHH386 pKa = 6.67DD387 pKa = 4.51EE388 pKa = 3.98ICFLHH393 pKa = 6.93LGRR396 pKa = 11.84MFGPMSGDD404 pKa = 3.34TYY406 pKa = 11.18RR407 pKa = 11.84RR408 pKa = 11.84IPSYY412 pKa = 8.85YY413 pKa = 7.57QHH415 pKa = 6.43MYY417 pKa = 10.32KK418 pKa = 10.25RR419 pKa = 11.84YY420 pKa = 8.1CKK422 pKa = 9.9KK423 pKa = 10.09VPHH426 pKa = 6.21MRR428 pKa = 11.84DD429 pKa = 3.04LLEE432 pKa = 4.14PVCYY436 pKa = 10.26GGMGGVTFDD445 pKa = 4.83EE446 pKa = 4.49YY447 pKa = 11.68LKK449 pKa = 9.85MVPVRR454 pKa = 11.84RR455 pKa = 11.84GSSLALHH462 pKa = 6.2HH463 pKa = 5.86QLVRR467 pKa = 11.84LGTPRR472 pKa = 11.84LAVMAYY478 pKa = 10.19DD479 pKa = 3.68VLVSLWFGWLTWSIPTCRR497 pKa = 11.84RR498 pKa = 11.84PEE500 pKa = 3.89HH501 pKa = 6.81PIGGFPRR508 pKa = 11.84VKK510 pKa = 10.63GKK512 pKa = 10.29LSCLLEE518 pKa = 4.06PRR520 pKa = 11.84MKK522 pKa = 10.12PWAPNVLEE530 pKa = 5.0FIRR533 pKa = 5.2

Molecular weight: 60.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

533

533

533

533.0

60.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.69 ± 0.0

3.752 ± 0.0

3.377 ± 0.0

4.878 ± 0.0

4.128 ± 0.0

7.129 ± 0.0

3.565 ± 0.0

4.503 ± 0.0

6.004 ± 0.0

11.257 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.752 ± 0.0

3.752 ± 0.0

5.441 ± 0.0

3.565 ± 0.0

6.191 ± 0.0

6.754 ± 0.0

4.315 ± 0.0

6.379 ± 0.0

1.876 ± 0.0

4.69 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski