Streptococcus phage Javan491
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6ADV4|A0A4D6ADV4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan491 OX=2548206 GN=Javan491_0013 PE=4 SV=1
MM1 pKa = 7.35 TPEE4 pKa = 3.67 QAEE7 pKa = 4.27 KK8 pKa = 10.98 AKK10 pKa = 10.36 IRR12 pKa = 11.84 AKK14 pKa = 10.52 QEE16 pKa = 3.83 LEE18 pKa = 4.0 TFSIYY23 pKa = 10.58 LDD25 pKa = 3.52 QAIDD29 pKa = 3.56 EE30 pKa = 4.76 LGGVLTSRR38 pKa = 11.84 EE39 pKa = 3.96 VFLAAGITYY48 pKa = 10.35 LGAGQTDD55 pKa = 2.47 IHH57 pKa = 6.4 AAVEE61 pKa = 4.48 GLCEE65 pKa = 4.1 QIQQ68 pKa = 3.34
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.524
IPC2_protein 4.546
IPC_protein 4.355
Toseland 4.215
ProMoST 4.444
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.139
Rodwell 4.202
Grimsley 4.139
Solomon 4.279
Lehninger 4.228
Nozaki 4.406
DTASelect 4.482
Thurlkill 4.228
EMBOSS 4.164
Sillero 4.457
Patrickios 3.719
IPC_peptide 4.279
IPC2_peptide 4.457
IPC2.peptide.svr19 4.37
Protein with the highest isoelectric point:
>tr|A0A4D6AIL6|A0A4D6AIL6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan491 OX=2548206 GN=Javan491_0020 PE=4 SV=1
MM1 pKa = 7.36 TKK3 pKa = 7.85 QHH5 pKa = 6.91 RR6 pKa = 11.84 EE7 pKa = 3.47 TLIWYY12 pKa = 7.66 RR13 pKa = 11.84 ASHH16 pKa = 5.21 QEE18 pKa = 3.99 RR19 pKa = 11.84 EE20 pKa = 4.0 RR21 pKa = 11.84 LLDD24 pKa = 4.03 FGLVDD29 pKa = 3.72 KK30 pKa = 10.63 SQYY33 pKa = 7.51 MTLLRR38 pKa = 11.84 QLRR41 pKa = 11.84 KK42 pKa = 9.91 KK43 pKa = 10.48 YY44 pKa = 10.73 AII46 pKa = 4.1
Molecular weight: 5.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.237
IPC2_protein 9.604
IPC_protein 10.028
Toseland 10.233
ProMoST 9.94
Dawson 10.423
Bjellqvist 10.101
Wikipedia 10.613
Rodwell 10.76
Grimsley 10.496
Solomon 10.482
Lehninger 10.452
Nozaki 10.189
DTASelect 10.101
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.335
Patrickios 10.54
IPC_peptide 10.482
IPC2_peptide 8.785
IPC2.peptide.svr19 8.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
10517
46
785
191.2
21.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.654 ± 0.509
0.656 ± 0.1
6.513 ± 0.253
7.331 ± 0.512
3.746 ± 0.175
6.732 ± 0.448
1.36 ± 0.15
6.922 ± 0.285
9.394 ± 0.424
8.31 ± 0.224
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.501 ± 0.148
5.296 ± 0.225
2.653 ± 0.203
3.87 ± 0.243
3.832 ± 0.3
6.513 ± 0.371
5.781 ± 0.221
6.133 ± 0.178
1.151 ± 0.126
3.651 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here