Capybara microvirus Cap1_SP_137
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4A0|A0A4P8W4A0_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_137 OX=2585385 PE=4 SV=1
MM1 pKa = 7.66 TEE3 pKa = 4.38 TISKK7 pKa = 9.67 IDD9 pKa = 3.77 EE10 pKa = 4.35 LRR12 pKa = 11.84 QQAYY16 pKa = 6.39 EE17 pKa = 4.0 TGDD20 pKa = 3.36 NVLRR24 pKa = 11.84 WHH26 pKa = 7.56 DD27 pKa = 3.81 EE28 pKa = 3.91 QEE30 pKa = 3.84 KK31 pKa = 11.12 DD32 pKa = 3.68 FLIVQEE38 pKa = 4.29 KK39 pKa = 9.2 NDD41 pKa = 3.67 YY42 pKa = 10.04 YY43 pKa = 11.44 KK44 pKa = 10.12 RR45 pKa = 11.84 TAAAAKK51 pKa = 9.93 GVTIYY56 pKa = 11.2 EE57 pKa = 4.33 MIQIYY62 pKa = 10.66 GSVDD66 pKa = 2.76 AAAAATGEE74 pKa = 4.47 VPASAMIDD82 pKa = 3.31 ASDD85 pKa = 3.7 VPQFGGNVDD94 pKa = 4.59 LDD96 pKa = 3.82 ALANLLLAKK105 pKa = 10.36 AQAAQQANNAAKK117 pKa = 8.9 TAQNTTTDD125 pKa = 3.41 KK126 pKa = 11.52 TEE128 pKa = 5.24 DD129 pKa = 3.54 NPQQ132 pKa = 2.95
Molecular weight: 14.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.429
IPC2_protein 4.317
IPC_protein 4.24
Toseland 4.05
ProMoST 4.38
Dawson 4.202
Bjellqvist 4.368
Wikipedia 4.113
Rodwell 4.062
Grimsley 3.961
Solomon 4.202
Lehninger 4.151
Nozaki 4.317
DTASelect 4.533
Thurlkill 4.075
EMBOSS 4.126
Sillero 4.355
Patrickios 3.872
IPC_peptide 4.202
IPC2_peptide 4.342
IPC2.peptide.svr19 4.289
Protein with the highest isoelectric point:
>tr|A0A4P8W4C0|A0A4P8W4C0_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_137 OX=2585385 PE=3 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 VACPPRR8 pKa = 11.84 AKK10 pKa = 10.35 NLKK13 pKa = 8.15 NACAPRR19 pKa = 11.84 HH20 pKa = 6.01 AIRR23 pKa = 11.84 AKK25 pKa = 10.41 KK26 pKa = 8.24 PTIKK30 pKa = 10.06 IFQSYY35 pKa = 10.3 LSIYY39 pKa = 10.19 LGARR43 pKa = 11.84 GQGAKK48 pKa = 10.16 RR49 pKa = 11.84 LPTNLPNEE57 pKa = 4.13 VLLFSLSPALSGLSLLRR74 pKa = 11.84 FLFVASVLALEE85 pKa = 4.78 CPSLKK90 pKa = 10.9 GNN92 pKa = 3.75
Molecular weight: 9.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.897
IPC_protein 10.657
Toseland 10.994
ProMoST 10.672
Dawson 11.052
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.389
Grimsley 11.096
Solomon 11.199
Lehninger 11.169
Nozaki 10.979
DTASelect 10.745
Thurlkill 10.979
EMBOSS 11.403
Sillero 10.994
Patrickios 11.14
IPC_peptide 11.213
IPC2_peptide 9.926
IPC2.peptide.svr19 8.406
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1454
70
565
161.6
18.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.428 ± 1.44
1.169 ± 0.394
5.227 ± 0.738
5.021 ± 0.518
4.402 ± 0.504
6.671 ± 0.939
1.719 ± 0.253
5.227 ± 0.794
6.809 ± 1.4
10.316 ± 1.113
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.132 ± 0.574
6.259 ± 0.944
4.677 ± 0.721
4.127 ± 0.685
5.708 ± 0.768
5.846 ± 0.719
5.915 ± 0.479
5.777 ± 0.693
1.1 ± 0.224
4.47 ± 0.778
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here