Gordonia phage RedWattleHog
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D5FSD4|A0A7D5FSD4_9CAUD Uncharacterized protein OS=Gordonia phage RedWattleHog OX=2743988 GN=201 PE=4 SV=1
MM1 pKa = 7.41 TSYY4 pKa = 11.26 LKK6 pKa = 10.85 LSEE9 pKa = 4.23 RR10 pKa = 11.84 PGLSFTAPEE19 pKa = 4.29 CNACLKK25 pKa = 10.59 EE26 pKa = 3.88 VDD28 pKa = 3.94 HH29 pKa = 7.56 DD30 pKa = 4.58 GDD32 pKa = 3.43 GWLCPSCGTSWPGNVMEE49 pKa = 5.28 SDD51 pKa = 3.62 GSDD54 pKa = 2.95 AQLFEE59 pKa = 4.42 EE60 pKa = 4.86 WSGEE64 pKa = 4.02 EE65 pKa = 5.08 LPGPTCKK72 pKa = 9.66 TDD74 pKa = 3.29 EE75 pKa = 4.93 AYY77 pKa = 10.67 LAAISPSDD85 pKa = 2.73 WWKK88 pKa = 9.85 RR89 pKa = 11.84 WPSCVYY95 pKa = 10.1 NQSDD99 pKa = 5.1 DD100 pKa = 5.15 DD101 pKa = 4.69 HH102 pKa = 7.14 DD103 pKa = 3.9 QQKK106 pKa = 11.07 DD107 pKa = 3.68 GNGG110 pKa = 3.23
Molecular weight: 12.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.787
IPC2_protein 4.012
IPC_protein 3.961
Toseland 3.77
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.681
Solomon 3.935
Lehninger 3.897
Nozaki 4.075
DTASelect 4.279
Thurlkill 3.821
EMBOSS 3.884
Sillero 4.088
Patrickios 0.998
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 3.956
Protein with the highest isoelectric point:
>tr|A0A7D5JHW0|A0A7D5JHW0_9CAUD Minor tail protein OS=Gordonia phage RedWattleHog OX=2743988 GN=47 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.01 RR3 pKa = 11.84 QRR5 pKa = 11.84 GQAIKK10 pKa = 10.31 PGTSSNGYY18 pKa = 8.98 QLAILNGKK26 pKa = 9.05 ARR28 pKa = 11.84 LIHH31 pKa = 6.05 HH32 pKa = 7.12 LVMEE36 pKa = 4.77 AFVGPRR42 pKa = 11.84 PDD44 pKa = 3.72 GADD47 pKa = 2.89 IRR49 pKa = 11.84 HH50 pKa = 5.94 WPDD53 pKa = 2.77 TSKK56 pKa = 11.58 SNNTLSNLSYY66 pKa = 9.48 GTRR69 pKa = 11.84 RR70 pKa = 11.84 QNIMDD75 pKa = 4.35 AVAAGTWNNRR85 pKa = 11.84 NTGKK89 pKa = 7.27 THH91 pKa = 7.36 CDD93 pKa = 3.0 RR94 pKa = 11.84 GHH96 pKa = 6.6 EE97 pKa = 4.06 FTPEE101 pKa = 3.47 NTYY104 pKa = 10.46 VWTGRR109 pKa = 11.84 TGRR112 pKa = 11.84 EE113 pKa = 3.42 EE114 pKa = 4.12 RR115 pKa = 11.84 QCRR118 pKa = 11.84 ACNRR122 pKa = 11.84 MRR124 pKa = 11.84 RR125 pKa = 11.84 QGQLL129 pKa = 2.91
Molecular weight: 14.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.663
IPC_protein 10.657
Toseland 10.76
ProMoST 10.584
Dawson 10.847
Bjellqvist 10.613
Wikipedia 11.096
Rodwell 10.921
Grimsley 10.891
Solomon 11.023
Lehninger 10.979
Nozaki 10.76
DTASelect 10.599
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 10.672
IPC_peptide 11.023
IPC2_peptide 9.882
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
212
0
212
43022
30
1149
202.9
22.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.565 ± 0.248
0.99 ± 0.081
7.073 ± 0.214
6.794 ± 0.278
2.864 ± 0.126
7.935 ± 0.221
2.155 ± 0.118
4.209 ± 0.122
3.728 ± 0.169
7.522 ± 0.17
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.113
3.428 ± 0.143
5.569 ± 0.163
3.768 ± 0.113
6.81 ± 0.201
6.313 ± 0.184
6.35 ± 0.32
7.399 ± 0.175
1.82 ± 0.108
3.243 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here