Gammaproteobacteria bacterium MFB021

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3307 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086D1Q0|A0A086D1Q0_9GAMM Uncharacterized protein OS=Gammaproteobacteria bacterium MFB021 OX=1492922 GN=GY26_15215 PE=4 SV=1
MM1 pKa = 7.35SVLAASIAMAMSAAVMADD19 pKa = 2.97ATQQYY24 pKa = 11.38ADD26 pKa = 4.04GNTNPSSTFQSSRR39 pKa = 11.84DD40 pKa = 3.99AIDD43 pKa = 3.71AMAGFNGNAGNAAEE57 pKa = 4.52SFIDD61 pKa = 3.45QTGYY65 pKa = 10.18NHH67 pKa = 7.52DD68 pKa = 4.17ANVVQSGNQWSRR80 pKa = 11.84ITQQDD85 pKa = 3.87ADD87 pKa = 3.26NMARR91 pKa = 11.84VRR93 pKa = 11.84QEE95 pKa = 3.55SGAEE99 pKa = 3.81NEE101 pKa = 4.75SIVDD105 pKa = 3.48QRR107 pKa = 11.84DD108 pKa = 3.2QANYY112 pKa = 11.17AEE114 pKa = 4.57VTQAGYY120 pKa = 11.2SNDD123 pKa = 3.84SIVSQEE129 pKa = 3.55GNYY132 pKa = 10.26FGARR136 pKa = 11.84NDD138 pKa = 3.2AYY140 pKa = 11.02VRR142 pKa = 11.84QNGSLNDD149 pKa = 3.79SYY151 pKa = 11.7IMQDD155 pKa = 2.85GHH157 pKa = 7.16KK158 pKa = 10.54NLANVNQQGDD168 pKa = 3.81QLDD171 pKa = 3.86SDD173 pKa = 4.21ILQDD177 pKa = 3.39GSYY180 pKa = 10.13NQAVVNQSGYY190 pKa = 10.34GHH192 pKa = 7.53DD193 pKa = 4.18SYY195 pKa = 11.9VSQDD199 pKa = 2.89GFANRR204 pKa = 11.84VNHH207 pKa = 5.7SQSGGWQNFAQTVQNGVGNVANVTQYY233 pKa = 11.74

Molecular weight: 25.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086D3X4|A0A086D3X4_9GAMM Flagellar protein FliL OS=Gammaproteobacteria bacterium MFB021 OX=1492922 GN=GY26_09535 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3307

0

3307

1054741

43

2699

318.9

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.101 ± 0.056

0.932 ± 0.015

5.838 ± 0.037

5.893 ± 0.049

3.433 ± 0.024

8.371 ± 0.042

2.383 ± 0.023

4.659 ± 0.033

2.542 ± 0.033

11.335 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.021

2.427 ± 0.028

4.945 ± 0.03

3.986 ± 0.028

7.309 ± 0.061

5.427 ± 0.034

5.06 ± 0.039

7.104 ± 0.04

1.485 ± 0.022

2.444 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski