Prevotella sp. S7 MS 2
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2144 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A099BXK4|A0A099BXK4_9BACT Uncharacterized protein OS=Prevotella sp. S7 MS 2 OX=1287488 GN=HMPREF0671_07660 PE=4 SV=1
MM1 pKa = 7.83 IYY3 pKa = 10.59 LILILIALGVFTALLGIISSLRR25 pKa = 11.84 GGGDD29 pKa = 4.23 DD30 pKa = 3.29 IVHH33 pKa = 7.29 PATASCATCTGDD45 pKa = 5.53 NDD47 pKa = 3.72 MCEE50 pKa = 3.96 QDD52 pKa = 4.61 CMLEE56 pKa = 3.98 AATKK60 pKa = 10.15 DD61 pKa = 2.96 IEE63 pKa = 4.35 YY64 pKa = 10.54 FDD66 pKa = 5.94 DD67 pKa = 4.27 EE68 pKa = 4.73 EE69 pKa = 5.86 LDD71 pKa = 4.02 AYY73 pKa = 10.64 HH74 pKa = 7.43 DD75 pKa = 4.12 RR76 pKa = 11.84 PSNQYY81 pKa = 10.74 SDD83 pKa = 3.91 TEE85 pKa = 3.89 ADD87 pKa = 3.5 EE88 pKa = 4.93 FSEE91 pKa = 4.7 IMYY94 pKa = 9.41 TMKK97 pKa = 10.62 PEE99 pKa = 3.95 EE100 pKa = 3.88 VKK102 pKa = 10.42 AWNRR106 pKa = 11.84 SLILRR111 pKa = 11.84 GINMPDD117 pKa = 3.26 QIKK120 pKa = 11.15 DD121 pKa = 3.39 EE122 pKa = 5.12 FIALAEE128 pKa = 4.09
Molecular weight: 14.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.694
ProMoST 4.024
Dawson 3.884
Bjellqvist 4.075
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.202
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.024
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A099C0N6|A0A099C0N6_9BACT Regulatory protein RecX OS=Prevotella sp. S7 MS 2 OX=1287488 GN=HMPREF0671_01590 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.23 VITMEE7 pKa = 4.02 SSAFRR12 pKa = 11.84 SLTEE16 pKa = 3.93 QIAEE20 pKa = 4.05 IAAHH24 pKa = 5.66 VRR26 pKa = 11.84 AASGDD31 pKa = 3.61 KK32 pKa = 10.39 KK33 pKa = 10.73 AASPDD38 pKa = 3.27 RR39 pKa = 11.84 LLTTRR44 pKa = 11.84 EE45 pKa = 3.83 AAHH48 pKa = 6.7 LLNVSTRR55 pKa = 11.84 TLQRR59 pKa = 11.84 MRR61 pKa = 11.84 SEE63 pKa = 3.67 QRR65 pKa = 11.84 IGYY68 pKa = 8.75 VVLRR72 pKa = 11.84 GKK74 pKa = 10.05 CRR76 pKa = 11.84 YY77 pKa = 8.65 RR78 pKa = 11.84 QSEE81 pKa = 3.85 IDD83 pKa = 4.11 RR84 pKa = 11.84 LLEE87 pKa = 4.02 ACAVNEE93 pKa = 4.46 DD94 pKa = 3.58 AATPQEE100 pKa = 4.3 LKK102 pKa = 10.6 RR103 pKa = 11.84 NHH105 pKa = 5.62 TLRR108 pKa = 11.84 TGGKK112 pKa = 8.07 PKK114 pKa = 10.3 GRR116 pKa = 11.84 RR117 pKa = 11.84 TT118 pKa = 3.39
Molecular weight: 13.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.502
IPC_protein 10.131
Toseland 10.687
ProMoST 10.335
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 10.965
Grimsley 10.789
Solomon 10.891
Lehninger 10.862
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.428
IPC2.peptide.svr19 8.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2144
0
2144
772140
30
2510
360.1
40.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.238 ± 0.052
1.223 ± 0.018
5.654 ± 0.037
6.075 ± 0.051
4.396 ± 0.034
6.877 ± 0.042
2.19 ± 0.02
6.782 ± 0.043
6.489 ± 0.046
9.078 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.986 ± 0.026
4.98 ± 0.055
3.625 ± 0.026
3.771 ± 0.03
4.87 ± 0.038
5.913 ± 0.042
5.739 ± 0.037
6.601 ± 0.034
1.237 ± 0.018
4.277 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here