Paracoccus phage vB_PmaS-R3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5A2H1|A0A0B5A2H1_9CAUD Putative HD-domain protein OS=Paracoccus phage vB_PmaS-R3 OX=2494563 PE=4 SV=1
MM1 pKa = 7.87TDD3 pKa = 3.23TPKK6 pKa = 10.98LGLTYY11 pKa = 10.74LEE13 pKa = 4.64ASQAQKK19 pKa = 10.81HH20 pKa = 4.76VTVNDD25 pKa = 3.88ALRR28 pKa = 11.84QLDD31 pKa = 3.87GLVQANVADD40 pKa = 4.38KK41 pKa = 11.03DD42 pKa = 3.65LTAAPGSPSTGDD54 pKa = 3.27AYY56 pKa = 9.9IVASGATGVWAGQDD70 pKa = 3.26DD71 pKa = 4.51KK72 pKa = 11.69LAVYY76 pKa = 10.24HH77 pKa = 5.84DD78 pKa = 4.18TAWYY82 pKa = 9.09FYY84 pKa = 10.11TPVAGWLVYY93 pKa = 9.67VTDD96 pKa = 3.59EE97 pKa = 4.63ADD99 pKa = 3.73FYY101 pKa = 11.37SYY103 pKa = 11.24NGSAWVTFLSTFGGPFLPMSGGNLTGPLGINGGTTDD139 pKa = 4.45ASNQLVANLDD149 pKa = 3.4AALFNNDD156 pKa = 2.57GDD158 pKa = 4.54SVVMKK163 pKa = 10.27LNKK166 pKa = 9.92NATGDD171 pKa = 3.79DD172 pKa = 3.71ASLSFQSAFTAYY184 pKa = 10.59AQMGLLGNNDD194 pKa = 2.82WSLKK198 pKa = 9.52VGASATTALVAKK210 pKa = 9.43QATGAVEE217 pKa = 4.35LTQHH221 pKa = 6.57PKK223 pKa = 10.24FSGYY227 pKa = 10.61CNYY230 pKa = 10.56DD231 pKa = 3.14QYY233 pKa = 11.82NAAGAWFKK241 pKa = 11.56VDD243 pKa = 3.84VNVLRR248 pKa = 11.84HH249 pKa = 5.94NDD251 pKa = 3.15QAAVSGGTFTAPHH264 pKa = 6.84DD265 pKa = 4.27GYY267 pKa = 11.26YY268 pKa = 10.68SFGGGVRR275 pKa = 11.84HH276 pKa = 7.0KK277 pKa = 11.02INGTPADD284 pKa = 3.88LVTIGFSVNGANPTPDD300 pKa = 3.24TQAMVGNAGWSADD313 pKa = 3.71DD314 pKa = 3.47NTFANTTAVLKK325 pKa = 10.63LSSGDD330 pKa = 4.07TVEE333 pKa = 3.98LWCYY337 pKa = 7.61FTTNDD342 pKa = 3.67AYY344 pKa = 10.29VTADD348 pKa = 3.12YY349 pKa = 11.14NYY351 pKa = 9.75FHH353 pKa = 7.28GVQIAA358 pKa = 3.03

Molecular weight: 37.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5A7I7|A0A0B5A7I7_9CAUD Primase OS=Paracoccus phage vB_PmaS-R3 OX=2494563 PE=4 SV=1
MM1 pKa = 7.77ADD3 pKa = 3.93ISDD6 pKa = 4.27KK7 pKa = 11.06LPPPKK12 pKa = 10.4RR13 pKa = 11.84CDD15 pKa = 3.19NCNSSRR21 pKa = 11.84IRR23 pKa = 11.84LHH25 pKa = 6.03KK26 pKa = 9.18NHH28 pKa = 6.43RR29 pKa = 11.84RR30 pKa = 11.84NRR32 pKa = 11.84NKK34 pKa = 10.12YY35 pKa = 8.09VCQRR39 pKa = 11.84CYY41 pKa = 11.32ASVYY45 pKa = 8.4CHH47 pKa = 7.0KK48 pKa = 9.98GTNIPLGYY56 pKa = 9.02MATAHH61 pKa = 5.28TRR63 pKa = 11.84YY64 pKa = 9.75LRR66 pKa = 11.84AKK68 pKa = 8.66AHH70 pKa = 5.94EE71 pKa = 4.51AFDD74 pKa = 3.81VLWRR78 pKa = 11.84HH79 pKa = 5.87SLLTRR84 pKa = 11.84DD85 pKa = 4.43EE86 pKa = 4.42AYY88 pKa = 9.9EE89 pKa = 3.7WLARR93 pKa = 11.84ILGIALEE100 pKa = 4.21NAHH103 pKa = 6.85IGRR106 pKa = 11.84LTDD109 pKa = 3.43AQLEE113 pKa = 4.25RR114 pKa = 11.84VIAKK118 pKa = 9.86SGRR121 pKa = 11.84HH122 pKa = 4.0YY123 pKa = 10.86RR124 pKa = 11.84RR125 pKa = 11.84VKK127 pKa = 9.91TRR129 pKa = 11.84TKK131 pKa = 10.2KK132 pKa = 10.36RR133 pKa = 11.84KK134 pKa = 8.36AVQSATYY141 pKa = 9.5RR142 pKa = 11.84RR143 pKa = 11.84QRR145 pKa = 11.84AYY147 pKa = 9.29TEE149 pKa = 3.69QRR151 pKa = 11.84KK152 pKa = 8.98RR153 pKa = 11.84RR154 pKa = 11.84HH155 pKa = 4.99GGKK158 pKa = 8.95WKK160 pKa = 7.53PTPNRR165 pKa = 11.84YY166 pKa = 7.98EE167 pKa = 3.75

Molecular weight: 19.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13050

75

1291

251.0

27.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.958 ± 0.713

0.866 ± 0.136

7.08 ± 0.279

5.709 ± 0.31

3.517 ± 0.162

8.314 ± 0.32

1.923 ± 0.18

4.054 ± 0.233

4.805 ± 0.339

7.594 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.138

4.184 ± 0.223

4.897 ± 0.296

3.785 ± 0.302

6.107 ± 0.387

5.226 ± 0.278

6.613 ± 0.308

7.172 ± 0.299

1.586 ± 0.167

3.471 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski