Amino acid dipepetide frequency for Nitrosomonas sp. Nm51

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.735AlaAla: 9.735 ± 0.148
1.087AlaCys: 1.087 ± 0.038
4.844AlaAsp: 4.844 ± 0.082
5.564AlaGlu: 5.564 ± 0.093
3.528AlaPhe: 3.528 ± 0.07
7.249AlaGly: 7.249 ± 0.113
2.167AlaHis: 2.167 ± 0.049
5.946AlaIle: 5.946 ± 0.093
3.747AlaLys: 3.747 ± 0.069
9.932AlaLeu: 9.932 ± 0.143
2.437AlaMet: 2.437 ± 0.056
3.146AlaAsn: 3.146 ± 0.06
2.985AlaPro: 2.985 ± 0.062
3.85AlaGln: 3.85 ± 0.071
5.119AlaArg: 5.119 ± 0.09
5.023AlaSer: 5.023 ± 0.081
4.241AlaThr: 4.241 ± 0.089
6.615AlaVal: 6.615 ± 0.095
1.115AlaTrp: 1.115 ± 0.038
2.553AlaTyr: 2.553 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.94CysAla: 0.94 ± 0.033
0.176CysCys: 0.176 ± 0.014
0.576CysAsp: 0.576 ± 0.028
0.551CysGlu: 0.551 ± 0.032
0.434CysPhe: 0.434 ± 0.021
0.961CysGly: 0.961 ± 0.034
0.345CysHis: 0.345 ± 0.023
0.699CysIle: 0.699 ± 0.029
0.42CysLys: 0.42 ± 0.024
1.022CysLeu: 1.022 ± 0.039
0.259CysMet: 0.259 ± 0.018
0.403CysAsn: 0.403 ± 0.022
0.489CysPro: 0.489 ± 0.024
0.355CysGln: 0.355 ± 0.021
0.684CysArg: 0.684 ± 0.032
0.624CysSer: 0.624 ± 0.033
0.517CysThr: 0.517 ± 0.022
0.678CysVal: 0.678 ± 0.027
0.126CysTrp: 0.126 ± 0.011
0.335CysTyr: 0.335 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
5.03AspAla: 5.03 ± 0.089
0.552AspCys: 0.552 ± 0.028
3.061AspAsp: 3.061 ± 0.081
3.583AspGlu: 3.583 ± 0.065
2.484AspPhe: 2.484 ± 0.054
3.823AspGly: 3.823 ± 0.095
1.223AspHis: 1.223 ± 0.043
3.743AspIle: 3.743 ± 0.067
2.556AspLys: 2.556 ± 0.063
5.139AspLeu: 5.139 ± 0.069
1.345AspMet: 1.345 ± 0.036
2.309AspAsn: 2.309 ± 0.06
2.457AspPro: 2.457 ± 0.06
2.123AspGln: 2.123 ± 0.05
2.932AspArg: 2.932 ± 0.06
2.987AspSer: 2.987 ± 0.061
3.088AspThr: 3.088 ± 0.081
3.437AspVal: 3.437 ± 0.064
0.871AspTrp: 0.871 ± 0.031
2.01AspTyr: 2.01 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
5.241GluAla: 5.241 ± 0.094
0.484GluCys: 0.484 ± 0.025
2.701GluAsp: 2.701 ± 0.054
3.22GluGlu: 3.22 ± 0.073
2.196GluPhe: 2.196 ± 0.053
2.981GluGly: 2.981 ± 0.058
1.442GluHis: 1.442 ± 0.046
4.789GluIle: 4.789 ± 0.071
3.963GluLys: 3.963 ± 0.082
5.888GluLeu: 5.888 ± 0.093
1.599GluMet: 1.599 ± 0.042
2.981GluAsn: 2.981 ± 0.063
2.113GluPro: 2.113 ± 0.053
3.132GluGln: 3.132 ± 0.068
3.494GluArg: 3.494 ± 0.069
3.496GluSer: 3.496 ± 0.074
3.659GluThr: 3.659 ± 0.065
3.223GluVal: 3.223 ± 0.071
0.742GluTrp: 0.742 ± 0.034
1.599GluTyr: 1.599 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.375PheAla: 3.375 ± 0.067
0.555PheCys: 0.555 ± 0.027
2.689PheAsp: 2.689 ± 0.064
2.433PheGlu: 2.433 ± 0.049
2.047PhePhe: 2.047 ± 0.053
3.002PheGly: 3.002 ± 0.063
0.879PheHis: 0.879 ± 0.033
2.618PheIle: 2.618 ± 0.06
1.702PheLys: 1.702 ± 0.046
3.794PheLeu: 3.794 ± 0.071
0.97PheMet: 0.97 ± 0.036
1.853PheAsn: 1.853 ± 0.035
1.683PhePro: 1.683 ± 0.052
1.302PheGln: 1.302 ± 0.038
1.953PheArg: 1.953 ± 0.045
3.098PheSer: 3.098 ± 0.055
2.236PheThr: 2.236 ± 0.063
2.671PheVal: 2.671 ± 0.058
0.54PheTrp: 0.54 ± 0.03
1.339PheTyr: 1.339 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
5.506GlyAla: 5.506 ± 0.103
0.899GlyCys: 0.899 ± 0.033
3.51GlyAsp: 3.51 ± 0.08
3.885GlyGlu: 3.885 ± 0.072
3.19GlyPhe: 3.19 ± 0.067
5.256GlyGly: 5.256 ± 0.118
1.796GlyHis: 1.796 ± 0.057
5.012GlyIle: 5.012 ± 0.08
3.874GlyLys: 3.874 ± 0.074
6.943GlyLeu: 6.943 ± 0.096
2.103GlyMet: 2.103 ± 0.052
2.934GlyAsn: 2.934 ± 0.086
1.861GlyPro: 1.861 ± 0.05
2.571GlyGln: 2.571 ± 0.06
3.881GlyArg: 3.881 ± 0.083
4.074GlySer: 4.074 ± 0.096
3.787GlyThr: 3.787 ± 0.112
4.649GlyVal: 4.649 ± 0.081
1.054GlyTrp: 1.054 ± 0.037
2.405GlyTyr: 2.405 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.427HisAla: 2.427 ± 0.054
0.328HisCys: 0.328 ± 0.019
1.429HisAsp: 1.429 ± 0.039
1.422HisGlu: 1.422 ± 0.044
1.096HisPhe: 1.096 ± 0.037
1.861HisGly: 1.861 ± 0.056
0.785HisHis: 0.785 ± 0.036
1.617HisIle: 1.617 ± 0.039
0.948HisLys: 0.948 ± 0.029
2.385HisLeu: 2.385 ± 0.058
0.573HisMet: 0.573 ± 0.023
0.943HisAsn: 0.943 ± 0.032
1.281HisPro: 1.281 ± 0.041
1.089HisGln: 1.089 ± 0.037
1.362HisArg: 1.362 ± 0.038
1.394HisSer: 1.394 ± 0.038
1.317HisThr: 1.317 ± 0.044
1.571HisVal: 1.571 ± 0.041
0.368HisTrp: 0.368 ± 0.019
0.92HisTyr: 0.92 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.615IleAla: 6.615 ± 0.099
0.683IleCys: 0.683 ± 0.029
4.326IleAsp: 4.326 ± 0.078
4.489IleGlu: 4.489 ± 0.083
2.46IlePhe: 2.46 ± 0.058
4.796IleGly: 4.796 ± 0.083
1.589IleHis: 1.589 ± 0.043
4.198IleIle: 4.198 ± 0.083
3.197IleLys: 3.197 ± 0.068
6.21IleLeu: 6.21 ± 0.095
1.419IleMet: 1.419 ± 0.038
2.933IleAsn: 2.933 ± 0.065
3.147IlePro: 3.147 ± 0.061
2.604IleGln: 2.604 ± 0.062
3.676IleArg: 3.676 ± 0.068
4.247IleSer: 4.247 ± 0.062
3.946IleThr: 3.946 ± 0.084
4.283IleVal: 4.283 ± 0.075
0.735IleTrp: 0.735 ± 0.03
1.812IleTyr: 1.812 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.05LysAla: 4.05 ± 0.082
0.328LysCys: 0.328 ± 0.024
2.205LysAsp: 2.205 ± 0.061
2.594LysGlu: 2.594 ± 0.07
1.431LysPhe: 1.431 ± 0.035
2.402LysGly: 2.402 ± 0.06
1.22LysHis: 1.22 ± 0.037
3.496LysIle: 3.496 ± 0.073
3.148LysLys: 3.148 ± 0.076
4.851LysLeu: 4.851 ± 0.077
1.217LysMet: 1.217 ± 0.036
2.434LysAsn: 2.434 ± 0.051
2.393LysPro: 2.393 ± 0.049
2.571LysGln: 2.571 ± 0.05
2.833LysArg: 2.833 ± 0.069
2.829LysSer: 2.829 ± 0.061
3.167LysThr: 3.167 ± 0.063
2.593LysVal: 2.593 ± 0.063
0.533LysTrp: 0.533 ± 0.023
1.187LysTyr: 1.187 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
9.716LeuAla: 9.716 ± 0.129
1.1LeuCys: 1.1 ± 0.036
5.727LeuAsp: 5.727 ± 0.08
5.869LeuGlu: 5.869 ± 0.098
4.229LeuPhe: 4.229 ± 0.087
6.593LeuGly: 6.593 ± 0.097
2.477LeuHis: 2.477 ± 0.058
6.724LeuIle: 6.724 ± 0.097
5.083LeuLys: 5.083 ± 0.077
11.057LeuLeu: 11.057 ± 0.15
2.396LeuMet: 2.396 ± 0.056
4.375LeuAsn: 4.375 ± 0.075
5.325LeuPro: 5.325 ± 0.088
4.14LeuGln: 4.14 ± 0.066
5.649LeuArg: 5.649 ± 0.089
7.125LeuSer: 7.125 ± 0.089
5.914LeuThr: 5.914 ± 0.087
6.115LeuVal: 6.115 ± 0.095
1.138LeuTrp: 1.138 ± 0.039
2.605LeuTyr: 2.605 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.179MetAla: 2.179 ± 0.052
0.184MetCys: 0.184 ± 0.013
1.223MetAsp: 1.223 ± 0.036
1.259MetGlu: 1.259 ± 0.041
0.788MetPhe: 0.788 ± 0.032
1.563MetGly: 1.563 ± 0.042
0.694MetHis: 0.694 ± 0.03
1.576MetIle: 1.576 ± 0.038
1.267MetLys: 1.267 ± 0.034
2.889MetLeu: 2.889 ± 0.058
0.676MetMet: 0.676 ± 0.032
1.137MetAsn: 1.137 ± 0.037
1.349MetPro: 1.349 ± 0.037
1.263MetGln: 1.263 ± 0.038
1.567MetArg: 1.567 ± 0.043
1.438MetSer: 1.438 ± 0.044
1.503MetThr: 1.503 ± 0.038
1.541MetVal: 1.541 ± 0.047
0.164MetTrp: 0.164 ± 0.013
0.485MetTyr: 0.485 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.676AsnAla: 3.676 ± 0.074
0.451AsnCys: 0.451 ± 0.026
2.278AsnAsp: 2.278 ± 0.076
2.362AsnGlu: 2.362 ± 0.054
1.6AsnPhe: 1.6 ± 0.048
2.929AsnGly: 2.929 ± 0.077
1.009AsnHis: 1.009 ± 0.03
2.818AsnIle: 2.818 ± 0.06
2.005AsnLys: 2.005 ± 0.047
4.025AsnLeu: 4.025 ± 0.067
0.946AsnMet: 0.946 ± 0.03
1.955AsnAsn: 1.955 ± 0.05
2.307AsnPro: 2.307 ± 0.057
1.913AsnGln: 1.913 ± 0.052
2.463AsnArg: 2.463 ± 0.048
2.397AsnSer: 2.397 ± 0.048
2.446AsnThr: 2.446 ± 0.061
2.461AsnVal: 2.461 ± 0.052
0.619AsnTrp: 0.619 ± 0.025
1.251AsnTyr: 1.251 ± 0.036
0.0AsnXaa: 0.0 ± 0.0
Pro
4.073ProAla: 4.073 ± 0.066
0.354ProCys: 0.354 ± 0.019
3.237ProAsp: 3.237 ± 0.066
3.419ProGlu: 3.419 ± 0.057
1.761ProPhe: 1.761 ± 0.043
3.565ProGly: 3.565 ± 0.066
0.999ProHis: 0.999 ± 0.033
2.236ProIle: 2.236 ± 0.054
1.648ProLys: 1.648 ± 0.042
4.105ProLeu: 4.105 ± 0.078
0.939ProMet: 0.939 ± 0.033
1.524ProAsn: 1.524 ± 0.042
1.798ProPro: 1.798 ± 0.047
1.724ProGln: 1.724 ± 0.046
1.843ProArg: 1.843 ± 0.04
2.169ProSer: 2.169 ± 0.05
1.715ProThr: 1.715 ± 0.046
4.02ProVal: 4.02 ± 0.072
0.55ProTrp: 0.55 ± 0.024
1.282ProTyr: 1.282 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
4.013GlnAla: 4.013 ± 0.071
0.409GlnCys: 0.409 ± 0.024
1.856GlnAsp: 1.856 ± 0.042
2.29GlnGlu: 2.29 ± 0.058
1.727GlnPhe: 1.727 ± 0.04
2.391GlnGly: 2.391 ± 0.058
1.256GlnHis: 1.256 ± 0.039
2.925GlnIle: 2.925 ± 0.06
2.137GlnLys: 2.137 ± 0.057
4.834GlnLeu: 4.834 ± 0.089
0.975GlnMet: 0.975 ± 0.033
1.84GlnAsn: 1.84 ± 0.046
1.854GlnPro: 1.854 ± 0.047
2.461GlnGln: 2.461 ± 0.069
2.568GlnArg: 2.568 ± 0.058
2.748GlnSer: 2.748 ± 0.059
2.479GlnThr: 2.479 ± 0.057
2.584GlnVal: 2.584 ± 0.055
0.601GlnTrp: 0.601 ± 0.025
1.258GlnTyr: 1.258 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
4.313ArgAla: 4.313 ± 0.089
0.571ArgCys: 0.571 ± 0.028
2.8ArgAsp: 2.8 ± 0.06
3.455ArgGlu: 3.455 ± 0.068
2.66ArgPhe: 2.66 ± 0.054
3.036ArgGly: 3.036 ± 0.064
1.678ArgHis: 1.678 ± 0.047
4.22ArgIle: 4.22 ± 0.073
2.807ArgLys: 2.807 ± 0.052
6.344ArgLeu: 6.344 ± 0.081
1.517ArgMet: 1.517 ± 0.05
2.448ArgAsn: 2.448 ± 0.051
2.098ArgPro: 2.098 ± 0.052
2.872ArgGln: 2.872 ± 0.057
3.379ArgArg: 3.379 ± 0.07
3.136ArgSer: 3.136 ± 0.058
2.575ArgThr: 2.575 ± 0.061
3.51ArgVal: 3.51 ± 0.063
0.788ArgTrp: 0.788 ± 0.029
2.137ArgTyr: 2.137 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
5.487SerAla: 5.487 ± 0.089
0.66SerCys: 0.66 ± 0.028
3.423SerAsp: 3.423 ± 0.072
3.533SerGlu: 3.533 ± 0.076
2.363SerPhe: 2.363 ± 0.06
5.377SerGly: 5.377 ± 0.081
1.415SerHis: 1.415 ± 0.034
4.082SerIle: 4.082 ± 0.066
2.573SerLys: 2.573 ± 0.063
5.978SerLeu: 5.978 ± 0.083
1.507SerMet: 1.507 ± 0.041
2.368SerAsn: 2.368 ± 0.06
2.476SerPro: 2.476 ± 0.052
2.372SerGln: 2.372 ± 0.056
3.484SerArg: 3.484 ± 0.069
3.659SerSer: 3.659 ± 0.075
3.004SerThr: 3.004 ± 0.066
4.121SerVal: 4.121 ± 0.068
0.74SerTrp: 0.74 ± 0.031
1.588SerTyr: 1.588 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
5.511ThrAla: 5.511 ± 0.103
0.535ThrCys: 0.535 ± 0.026
3.131ThrAsp: 3.131 ± 0.076
3.175ThrGlu: 3.175 ± 0.059
1.985ThrPhe: 1.985 ± 0.048
4.795ThrGly: 4.795 ± 0.105
1.417ThrHis: 1.417 ± 0.043
3.472ThrIle: 3.472 ± 0.068
1.812ThrLys: 1.812 ± 0.043
6.019ThrLeu: 6.019 ± 0.102
1.106ThrMet: 1.106 ± 0.033
1.835ThrAsn: 1.835 ± 0.056
2.663ThrPro: 2.663 ± 0.052
2.261ThrGln: 2.261 ± 0.048
2.938ThrArg: 2.938 ± 0.071
2.817ThrSer: 2.817 ± 0.065
2.747ThrThr: 2.747 ± 0.073
4.322ThrVal: 4.322 ± 0.075
0.572ThrTrp: 0.572 ± 0.026
1.445ThrTyr: 1.445 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
5.593ValAla: 5.593 ± 0.087
0.701ValCys: 0.701 ± 0.03
3.56ValAsp: 3.56 ± 0.069
3.719ValGlu: 3.719 ± 0.073
2.878ValPhe: 2.878 ± 0.058
3.821ValGly: 3.821 ± 0.071
1.475ValHis: 1.475 ± 0.045
4.708ValIle: 4.708 ± 0.071
2.876ValLys: 2.876 ± 0.064
6.958ValLeu: 6.958 ± 0.086
1.855ValMet: 1.855 ± 0.045
2.933ValAsn: 2.933 ± 0.059
2.744ValPro: 2.744 ± 0.066
2.33ValGln: 2.33 ± 0.056
3.543ValArg: 3.543 ± 0.075
4.498ValSer: 4.498 ± 0.061
4.055ValThr: 4.055 ± 0.075
4.536ValVal: 4.536 ± 0.087
0.806ValTrp: 0.806 ± 0.031
1.867ValTyr: 1.867 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.808TrpAla: 0.808 ± 0.031
0.16TrpCys: 0.16 ± 0.013
0.551TrpAsp: 0.551 ± 0.024
0.654TrpGlu: 0.654 ± 0.026
0.527TrpPhe: 0.527 ± 0.026
0.676TrpGly: 0.676 ± 0.03
0.416TrpHis: 0.416 ± 0.023
0.881TrpIle: 0.881 ± 0.037
0.579TrpLys: 0.579 ± 0.028
1.788TrpLeu: 1.788 ± 0.047
0.34TrpMet: 0.34 ± 0.021
0.507TrpAsn: 0.507 ± 0.022
0.507TrpPro: 0.507 ± 0.022
0.77TrpGln: 0.77 ± 0.028
0.949TrpArg: 0.949 ± 0.033
0.708TrpSer: 0.708 ± 0.032
0.553TrpThr: 0.553 ± 0.023
0.811TrpVal: 0.811 ± 0.031
0.196TrpTrp: 0.196 ± 0.014
0.359TrpTyr: 0.359 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.597TyrAla: 2.597 ± 0.058
0.378TyrCys: 0.378 ± 0.02
1.626TyrAsp: 1.626 ± 0.048
1.465TyrGlu: 1.465 ± 0.039
1.354TyrPhe: 1.354 ± 0.039
2.116TyrGly: 2.116 ± 0.046
0.834TyrHis: 0.834 ± 0.031
1.606TyrIle: 1.606 ± 0.042
1.139TyrLys: 1.139 ± 0.035
3.195TyrLeu: 3.195 ± 0.066
0.565TyrMet: 0.565 ± 0.031
1.053TyrAsn: 1.053 ± 0.038
1.431TyrPro: 1.431 ± 0.039
1.506TyrGln: 1.506 ± 0.039
2.026TyrArg: 2.026 ± 0.05
1.744TyrSer: 1.744 ± 0.044
1.607TyrThr: 1.607 ± 0.046
1.714TyrVal: 1.714 ± 0.04
0.482TyrTrp: 0.482 ± 0.026
1.008TyrTyr: 1.008 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2986 proteins (914779 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski