Amino acid dipepetide frequency for Pseudomonas phage KPP21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.446AlaAla: 10.446 ± 1.238
0.817AlaCys: 0.817 ± 0.262
5.072AlaAsp: 5.072 ± 0.511
7.394AlaGlu: 7.394 ± 0.663
3.095AlaPhe: 3.095 ± 0.342
6.491AlaGly: 6.491 ± 0.813
1.848AlaHis: 1.848 ± 0.265
4.943AlaIle: 4.943 ± 0.497
6.577AlaLys: 6.577 ± 0.706
7.91AlaLeu: 7.91 ± 0.634
2.278AlaMet: 2.278 ± 0.28
3.826AlaAsn: 3.826 ± 0.491
2.751AlaPro: 2.751 ± 0.472
4.643AlaGln: 4.643 ± 0.718
4.643AlaArg: 4.643 ± 0.527
5.373AlaSer: 5.373 ± 0.697
5.244AlaThr: 5.244 ± 0.605
6.663AlaVal: 6.663 ± 0.643
1.161AlaTrp: 1.161 ± 0.261
2.837AlaTyr: 2.837 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
0.43CysAla: 0.43 ± 0.149
0.129CysCys: 0.129 ± 0.08
0.559CysAsp: 0.559 ± 0.164
0.989CysGlu: 0.989 ± 0.307
0.086CysPhe: 0.086 ± 0.067
0.989CysGly: 0.989 ± 0.291
0.301CysHis: 0.301 ± 0.136
0.559CysIle: 0.559 ± 0.186
0.86CysLys: 0.86 ± 0.271
0.645CysLeu: 0.645 ± 0.221
0.129CysMet: 0.129 ± 0.078
0.559CysAsn: 0.559 ± 0.178
0.43CysPro: 0.43 ± 0.155
0.301CysGln: 0.301 ± 0.113
0.387CysArg: 0.387 ± 0.14
0.645CysSer: 0.645 ± 0.194
0.516CysThr: 0.516 ± 0.176
0.817CysVal: 0.817 ± 0.236
0.215CysTrp: 0.215 ± 0.105
0.387CysTyr: 0.387 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
6.706AspAla: 6.706 ± 0.887
0.516AspCys: 0.516 ± 0.15
2.837AspAsp: 2.837 ± 0.328
3.697AspGlu: 3.697 ± 0.454
2.794AspPhe: 2.794 ± 0.345
4.041AspGly: 4.041 ± 0.491
1.29AspHis: 1.29 ± 0.277
2.88AspIle: 2.88 ± 0.293
3.224AspLys: 3.224 ± 0.36
6.061AspLeu: 6.061 ± 0.517
1.419AspMet: 1.419 ± 0.273
2.063AspAsn: 2.063 ± 0.265
2.966AspPro: 2.966 ± 0.391
2.235AspGln: 2.235 ± 0.315
2.708AspArg: 2.708 ± 0.319
3.654AspSer: 3.654 ± 0.402
3.052AspThr: 3.052 ± 0.384
2.88AspVal: 2.88 ± 0.368
0.903AspTrp: 0.903 ± 0.218
2.063AspTyr: 2.063 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
8.167GluAla: 8.167 ± 0.93
0.645GluCys: 0.645 ± 0.179
4.17GluAsp: 4.17 ± 0.519
5.846GluGlu: 5.846 ± 0.573
2.235GluPhe: 2.235 ± 0.305
4.557GluGly: 4.557 ± 0.487
1.29GluHis: 1.29 ± 0.228
3.568GluIle: 3.568 ± 0.436
3.224GluLys: 3.224 ± 0.435
6.878GluLeu: 6.878 ± 0.525
2.493GluMet: 2.493 ± 0.371
2.063GluAsn: 2.063 ± 0.31
1.591GluPro: 1.591 ± 0.235
3.138GluGln: 3.138 ± 0.573
4.17GluArg: 4.17 ± 0.475
4.772GluSer: 4.772 ± 0.492
3.525GluThr: 3.525 ± 0.487
4.9GluVal: 4.9 ± 0.474
1.161GluTrp: 1.161 ± 0.284
2.192GluTyr: 2.192 ± 0.287
0.0GluXaa: 0.0 ± 0.0
Phe
2.149PheAla: 2.149 ± 0.35
0.172PheCys: 0.172 ± 0.095
2.923PheAsp: 2.923 ± 0.291
2.149PheGlu: 2.149 ± 0.313
1.548PhePhe: 1.548 ± 0.302
2.106PheGly: 2.106 ± 0.324
0.946PheHis: 0.946 ± 0.184
2.02PheIle: 2.02 ± 0.333
2.708PheLys: 2.708 ± 0.351
3.009PheLeu: 3.009 ± 0.278
1.032PheMet: 1.032 ± 0.216
2.278PheAsn: 2.278 ± 0.336
1.118PhePro: 1.118 ± 0.223
1.548PheGln: 1.548 ± 0.268
2.321PheArg: 2.321 ± 0.306
2.02PheSer: 2.02 ± 0.355
2.063PheThr: 2.063 ± 0.399
2.622PheVal: 2.622 ± 0.313
0.602PheTrp: 0.602 ± 0.188
0.903PheTyr: 0.903 ± 0.212
0.0PheXaa: 0.0 ± 0.0
Gly
5.717GlyAla: 5.717 ± 0.56
0.473GlyCys: 0.473 ± 0.175
3.869GlyAsp: 3.869 ± 0.437
3.955GlyGlu: 3.955 ± 0.453
3.783GlyPhe: 3.783 ± 0.39
4.342GlyGly: 4.342 ± 0.567
1.032GlyHis: 1.032 ± 0.234
2.966GlyIle: 2.966 ± 0.399
4.342GlyLys: 4.342 ± 0.426
6.448GlyLeu: 6.448 ± 0.544
2.536GlyMet: 2.536 ± 0.313
2.923GlyAsn: 2.923 ± 0.331
1.848GlyPro: 1.848 ± 0.271
2.966GlyGln: 2.966 ± 0.416
2.579GlyArg: 2.579 ± 0.293
3.912GlySer: 3.912 ± 0.267
3.955GlyThr: 3.955 ± 0.431
5.545GlyVal: 5.545 ± 0.543
0.731GlyTrp: 0.731 ± 0.137
2.837GlyTyr: 2.837 ± 0.377
0.0GlyXaa: 0.0 ± 0.0
His
1.762HisAla: 1.762 ± 0.306
0.086HisCys: 0.086 ± 0.066
1.333HisAsp: 1.333 ± 0.239
1.419HisGlu: 1.419 ± 0.279
1.204HisPhe: 1.204 ± 0.251
1.118HisGly: 1.118 ± 0.203
0.602HisHis: 0.602 ± 0.2
1.075HisIle: 1.075 ± 0.266
1.376HisLys: 1.376 ± 0.251
2.02HisLeu: 2.02 ± 0.413
0.86HisMet: 0.86 ± 0.175
0.946HisAsn: 0.946 ± 0.17
0.817HisPro: 0.817 ± 0.245
0.989HisGln: 0.989 ± 0.208
0.903HisArg: 0.903 ± 0.178
1.118HisSer: 1.118 ± 0.268
1.161HisThr: 1.161 ± 0.18
1.333HisVal: 1.333 ± 0.316
0.258HisTrp: 0.258 ± 0.1
1.118HisTyr: 1.118 ± 0.287
0.0HisXaa: 0.0 ± 0.0
Ile
3.826IleAla: 3.826 ± 0.356
0.688IleCys: 0.688 ± 0.168
3.181IleAsp: 3.181 ± 0.516
3.267IleGlu: 3.267 ± 0.439
1.376IlePhe: 1.376 ± 0.226
3.138IleGly: 3.138 ± 0.313
1.247IleHis: 1.247 ± 0.256
1.891IleIle: 1.891 ± 0.365
2.923IleLys: 2.923 ± 0.397
4.127IleLeu: 4.127 ± 0.419
1.333IleMet: 1.333 ± 0.212
2.88IleAsn: 2.88 ± 0.344
2.751IlePro: 2.751 ± 0.357
2.106IleGln: 2.106 ± 0.331
3.138IleArg: 3.138 ± 0.369
2.837IleSer: 2.837 ± 0.363
2.794IleThr: 2.794 ± 0.284
3.224IleVal: 3.224 ± 0.376
0.301IleTrp: 0.301 ± 0.097
1.548IleTyr: 1.548 ± 0.251
0.0IleXaa: 0.0 ± 0.0
Lys
6.878LysAla: 6.878 ± 0.641
0.473LysCys: 0.473 ± 0.163
3.439LysAsp: 3.439 ± 0.354
4.857LysGlu: 4.857 ± 0.67
1.848LysPhe: 1.848 ± 0.26
3.869LysGly: 3.869 ± 0.441
1.161LysHis: 1.161 ± 0.306
2.45LysIle: 2.45 ± 0.408
3.697LysLys: 3.697 ± 0.57
5.545LysLeu: 5.545 ± 0.659
1.505LysMet: 1.505 ± 0.279
2.708LysAsn: 2.708 ± 0.272
2.708LysPro: 2.708 ± 0.342
3.138LysGln: 3.138 ± 0.34
2.88LysArg: 2.88 ± 0.297
3.138LysSer: 3.138 ± 0.355
3.439LysThr: 3.439 ± 0.331
4.428LysVal: 4.428 ± 0.545
0.559LysTrp: 0.559 ± 0.141
2.751LysTyr: 2.751 ± 0.42
0.0LysXaa: 0.0 ± 0.0
Leu
7.867LeuAla: 7.867 ± 0.547
1.247LeuCys: 1.247 ± 0.335
5.803LeuAsp: 5.803 ± 0.593
5.674LeuGlu: 5.674 ± 0.487
2.966LeuPhe: 2.966 ± 0.354
5.588LeuGly: 5.588 ± 0.537
1.977LeuHis: 1.977 ± 0.247
4.514LeuIle: 4.514 ± 0.51
6.276LeuLys: 6.276 ± 0.705
8.769LeuLeu: 8.769 ± 0.833
2.794LeuMet: 2.794 ± 0.297
4.342LeuAsn: 4.342 ± 0.376
4.557LeuPro: 4.557 ± 0.516
3.009LeuGln: 3.009 ± 0.301
5.975LeuArg: 5.975 ± 0.615
6.147LeuSer: 6.147 ± 0.566
4.557LeuThr: 4.557 ± 0.491
6.018LeuVal: 6.018 ± 0.566
1.161LeuTrp: 1.161 ± 0.293
3.138LeuTyr: 3.138 ± 0.434
0.0LeuXaa: 0.0 ± 0.0
Met
3.138MetAla: 3.138 ± 0.408
0.559MetCys: 0.559 ± 0.195
1.934MetAsp: 1.934 ± 0.3
2.665MetGlu: 2.665 ± 0.346
0.645MetPhe: 0.645 ± 0.142
1.934MetGly: 1.934 ± 0.34
0.903MetHis: 0.903 ± 0.255
1.591MetIle: 1.591 ± 0.243
1.891MetLys: 1.891 ± 0.354
2.923MetLeu: 2.923 ± 0.437
0.946MetMet: 0.946 ± 0.215
1.032MetAsn: 1.032 ± 0.281
1.462MetPro: 1.462 ± 0.267
1.548MetGln: 1.548 ± 0.269
1.419MetArg: 1.419 ± 0.262
1.934MetSer: 1.934 ± 0.311
2.149MetThr: 2.149 ± 0.3
1.762MetVal: 1.762 ± 0.293
0.258MetTrp: 0.258 ± 0.094
0.774MetTyr: 0.774 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
4.17AsnAla: 4.17 ± 0.426
0.43AsnCys: 0.43 ± 0.174
1.676AsnAsp: 1.676 ± 0.312
2.407AsnGlu: 2.407 ± 0.317
1.333AsnPhe: 1.333 ± 0.231
3.138AsnGly: 3.138 ± 0.447
0.946AsnHis: 0.946 ± 0.205
2.536AsnIle: 2.536 ± 0.344
2.708AsnLys: 2.708 ± 0.324
4.643AsnLeu: 4.643 ± 0.444
1.676AsnMet: 1.676 ± 0.288
1.891AsnAsn: 1.891 ± 0.268
2.235AsnPro: 2.235 ± 0.279
2.364AsnGln: 2.364 ± 0.349
2.278AsnArg: 2.278 ± 0.331
2.622AsnSer: 2.622 ± 0.379
2.751AsnThr: 2.751 ± 0.365
2.407AsnVal: 2.407 ± 0.289
0.645AsnTrp: 0.645 ± 0.266
1.505AsnTyr: 1.505 ± 0.253
0.0AsnXaa: 0.0 ± 0.0
Pro
3.611ProAla: 3.611 ± 0.489
0.172ProCys: 0.172 ± 0.085
2.235ProAsp: 2.235 ± 0.268
3.482ProGlu: 3.482 ± 0.389
1.462ProPhe: 1.462 ± 0.253
3.052ProGly: 3.052 ± 0.446
0.645ProHis: 0.645 ± 0.176
1.633ProIle: 1.633 ± 0.226
1.762ProLys: 1.762 ± 0.305
3.095ProLeu: 3.095 ± 0.378
1.247ProMet: 1.247 ± 0.233
1.891ProAsn: 1.891 ± 0.341
1.161ProPro: 1.161 ± 0.298
1.462ProGln: 1.462 ± 0.255
1.333ProArg: 1.333 ± 0.212
2.106ProSer: 2.106 ± 0.249
2.708ProThr: 2.708 ± 0.292
3.052ProVal: 3.052 ± 0.399
0.645ProTrp: 0.645 ± 0.197
1.075ProTyr: 1.075 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
4.986GlnAla: 4.986 ± 0.746
0.344GlnCys: 0.344 ± 0.116
2.45GlnAsp: 2.45 ± 0.33
3.482GlnGlu: 3.482 ± 0.478
1.204GlnPhe: 1.204 ± 0.172
2.665GlnGly: 2.665 ± 0.373
0.946GlnHis: 0.946 ± 0.225
2.364GlnIle: 2.364 ± 0.375
2.923GlnLys: 2.923 ± 0.355
4.342GlnLeu: 4.342 ± 0.391
1.419GlnMet: 1.419 ± 0.241
1.376GlnAsn: 1.376 ± 0.238
1.333GlnPro: 1.333 ± 0.292
2.106GlnGln: 2.106 ± 0.348
2.364GlnArg: 2.364 ± 0.42
2.106GlnSer: 2.106 ± 0.29
2.235GlnThr: 2.235 ± 0.317
3.396GlnVal: 3.396 ± 0.474
0.688GlnTrp: 0.688 ± 0.175
1.333GlnTyr: 1.333 ± 0.214
0.0GlnXaa: 0.0 ± 0.0
Arg
3.525ArgAla: 3.525 ± 0.359
0.43ArgCys: 0.43 ± 0.166
3.095ArgAsp: 3.095 ± 0.453
4.256ArgGlu: 4.256 ± 0.655
2.02ArgPhe: 2.02 ± 0.36
3.31ArgGly: 3.31 ± 0.394
0.688ArgHis: 0.688 ± 0.2
1.891ArgIle: 1.891 ± 0.251
3.439ArgLys: 3.439 ± 0.322
4.857ArgLeu: 4.857 ± 0.424
1.891ArgMet: 1.891 ± 0.247
2.966ArgAsn: 2.966 ± 0.333
1.462ArgPro: 1.462 ± 0.251
2.235ArgGln: 2.235 ± 0.341
3.439ArgArg: 3.439 ± 0.403
3.267ArgSer: 3.267 ± 0.321
2.837ArgThr: 2.837 ± 0.362
4.342ArgVal: 4.342 ± 0.437
0.688ArgTrp: 0.688 ± 0.183
2.02ArgTyr: 2.02 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
5.287SerAla: 5.287 ± 0.49
0.516SerCys: 0.516 ± 0.184
3.353SerAsp: 3.353 ± 0.364
3.826SerGlu: 3.826 ± 0.369
1.977SerPhe: 1.977 ± 0.363
4.514SerGly: 4.514 ± 0.413
1.333SerHis: 1.333 ± 0.237
3.267SerIle: 3.267 ± 0.411
3.439SerLys: 3.439 ± 0.4
5.674SerLeu: 5.674 ± 0.495
2.02SerMet: 2.02 ± 0.253
3.439SerAsn: 3.439 ± 0.387
1.934SerPro: 1.934 ± 0.256
2.536SerGln: 2.536 ± 0.342
3.095SerArg: 3.095 ± 0.385
2.88SerSer: 2.88 ± 0.386
3.095SerThr: 3.095 ± 0.38
3.439SerVal: 3.439 ± 0.402
0.946SerTrp: 0.946 ± 0.22
2.149SerTyr: 2.149 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
5.674ThrAla: 5.674 ± 0.619
0.645ThrCys: 0.645 ± 0.159
3.138ThrAsp: 3.138 ± 0.377
3.568ThrGlu: 3.568 ± 0.394
2.149ThrPhe: 2.149 ± 0.268
3.955ThrGly: 3.955 ± 0.501
1.505ThrHis: 1.505 ± 0.37
3.181ThrIle: 3.181 ± 0.457
3.611ThrLys: 3.611 ± 0.449
4.6ThrLeu: 4.6 ± 0.439
1.762ThrMet: 1.762 ± 0.334
2.708ThrAsn: 2.708 ± 0.39
2.708ThrPro: 2.708 ± 0.394
2.493ThrGln: 2.493 ± 0.317
2.407ThrArg: 2.407 ± 0.286
2.966ThrSer: 2.966 ± 0.43
2.321ThrThr: 2.321 ± 0.325
3.697ThrVal: 3.697 ± 0.358
0.731ThrTrp: 0.731 ± 0.201
1.548ThrTyr: 1.548 ± 0.249
0.0ThrXaa: 0.0 ± 0.0
Val
6.319ValAla: 6.319 ± 0.913
0.903ValCys: 0.903 ± 0.229
4.213ValAsp: 4.213 ± 0.448
4.299ValGlu: 4.299 ± 0.401
2.192ValPhe: 2.192 ± 0.339
4.557ValGly: 4.557 ± 0.491
1.591ValHis: 1.591 ± 0.235
3.224ValIle: 3.224 ± 0.391
4.041ValLys: 4.041 ± 0.384
6.018ValLeu: 6.018 ± 0.544
2.364ValMet: 2.364 ± 0.35
2.622ValAsn: 2.622 ± 0.334
2.235ValPro: 2.235 ± 0.356
3.31ValGln: 3.31 ± 0.306
3.568ValArg: 3.568 ± 0.369
4.385ValSer: 4.385 ± 0.448
4.299ValThr: 4.299 ± 0.4
3.912ValVal: 3.912 ± 0.523
1.161ValTrp: 1.161 ± 0.235
2.493ValTyr: 2.493 ± 0.472
0.0ValXaa: 0.0 ± 0.0
Trp
0.989TrpAla: 0.989 ± 0.215
0.172TrpCys: 0.172 ± 0.089
0.817TrpAsp: 0.817 ± 0.244
1.161TrpGlu: 1.161 ± 0.212
0.516TrpPhe: 0.516 ± 0.164
1.118TrpGly: 1.118 ± 0.21
0.258TrpHis: 0.258 ± 0.117
0.774TrpIle: 0.774 ± 0.159
0.645TrpLys: 0.645 ± 0.186
1.075TrpLeu: 1.075 ± 0.304
0.516TrpMet: 0.516 ± 0.164
0.516TrpAsn: 0.516 ± 0.163
0.473TrpPro: 0.473 ± 0.16
0.559TrpGln: 0.559 ± 0.181
0.731TrpArg: 0.731 ± 0.174
0.86TrpSer: 0.86 ± 0.163
0.645TrpThr: 0.645 ± 0.188
1.161TrpVal: 1.161 ± 0.273
0.258TrpTrp: 0.258 ± 0.124
0.43TrpTyr: 0.43 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.364TyrAla: 2.364 ± 0.333
0.516TyrCys: 0.516 ± 0.173
1.891TyrAsp: 1.891 ± 0.243
2.192TyrGlu: 2.192 ± 0.326
1.805TyrPhe: 1.805 ± 0.347
2.278TyrGly: 2.278 ± 0.434
0.989TyrHis: 0.989 ± 0.236
1.29TyrIle: 1.29 ± 0.259
1.805TyrLys: 1.805 ± 0.381
3.74TyrLeu: 3.74 ± 0.469
1.204TyrMet: 1.204 ± 0.254
1.333TyrAsn: 1.333 ± 0.257
1.29TyrPro: 1.29 ± 0.305
1.376TyrGln: 1.376 ± 0.263
2.321TyrArg: 2.321 ± 0.32
1.977TyrSer: 1.977 ± 0.39
2.02TyrThr: 2.02 ± 0.242
2.106TyrVal: 2.106 ± 0.42
0.602TyrTrp: 0.602 ± 0.194
1.633TyrTyr: 1.633 ± 0.382
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 113 proteins (23264 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski