Bacillus phage vB_BtS_BMBtp16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0T691|A0A1B0T691_9CAUD Head-tail joining protein OS=Bacillus phage vB_BtS_BMBtp16 OX=1701848 GN=BMBtp4_7 PE=4 SV=1
MM1 pKa = 7.86EE2 pKa = 5.98DD3 pKa = 4.45LIEE6 pKa = 4.29KK7 pKa = 9.93LKK9 pKa = 11.09SHH11 pKa = 6.04IHH13 pKa = 5.44WEE15 pKa = 4.01EE16 pKa = 4.3GMDD19 pKa = 3.43EE20 pKa = 4.39TMLSFYY26 pKa = 9.42ITQAKK31 pKa = 9.16TYY33 pKa = 9.99VKK35 pKa = 10.51NATGKK40 pKa = 7.1QTEE43 pKa = 4.14YY44 pKa = 10.63LIIMVAGLIYY54 pKa = 10.15DD55 pKa = 3.59YY56 pKa = 10.82RR57 pKa = 11.84VSEE60 pKa = 4.43KK61 pKa = 10.52EE62 pKa = 3.91LEE64 pKa = 4.19QALDD68 pKa = 3.3ALTPFFVQEE77 pKa = 3.96VYY79 pKa = 11.13ADD81 pKa = 3.86EE82 pKa = 4.92EE83 pKa = 4.3KK84 pKa = 11.3DD85 pKa = 3.52EE86 pKa = 4.21

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0T6B1|A0A1B0T6B1_9CAUD Uncharacterized protein OS=Bacillus phage vB_BtS_BMBtp16 OX=1701848 GN=BMBtp4_21 PE=4 SV=1
MM1 pKa = 7.31MEE3 pKa = 4.29YY4 pKa = 10.69LLSLSQDD11 pKa = 3.57LLRR14 pKa = 11.84VFLTIVVTAYY24 pKa = 10.72ANKK27 pKa = 9.68FANNLFKK34 pKa = 10.58KK35 pKa = 10.07SKK37 pKa = 9.67RR38 pKa = 11.84ATPRR42 pKa = 11.84RR43 pKa = 11.84QKK45 pKa = 10.4QGSSRR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 9.02

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

9368

37

1174

199.3

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.693 ± 0.521

0.63 ± 0.165

5.786 ± 0.209

7.782 ± 0.517

3.992 ± 0.15

6.33 ± 0.348

1.452 ± 0.191

7.173 ± 0.27

9.116 ± 0.446

8.177 ± 0.436

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.946 ± 0.238

5.658 ± 0.258

2.85 ± 0.204

3.982 ± 0.339

4.152 ± 0.347

5.946 ± 0.265

5.839 ± 0.313

6.223 ± 0.313

1.398 ± 0.195

3.875 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski