Salmonella phage Mutine
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5BPA4|A0A2H5BPA4_9CAUD Uncharacterized protein OS=Salmonella phage Mutine OX=2054274 GN=CPT_Mutine_023 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.25 LKK4 pKa = 10.61 KK5 pKa = 10.13 DD6 pKa = 3.4 VLFYY10 pKa = 11.13 KK11 pKa = 10.36 IMQVFAVTTMAFSLSACATLLDD33 pKa = 4.79 SVDD36 pKa = 3.9 LDD38 pKa = 3.99 APTFTNQQAVNKK50 pKa = 9.85 MEE52 pKa = 4.2 DD53 pKa = 3.51 TIKK56 pKa = 11.04 AHH58 pKa = 6.87 AALDD62 pKa = 3.77 NTTPGPLQTVCNYY75 pKa = 10.58 DD76 pKa = 3.97 DD77 pKa = 5.54 SIQEE81 pKa = 4.24 DD82 pKa = 3.93 EE83 pKa = 4.95 TYY85 pKa = 10.8 HH86 pKa = 5.37 CTTYY90 pKa = 10.81 VKK92 pKa = 10.2 EE93 pKa = 4.33 SSVVLYY99 pKa = 10.72 ADD101 pKa = 3.77 CTEE104 pKa = 4.32 EE105 pKa = 3.89 QCTATGYY112 pKa = 10.78 DD113 pKa = 3.34 QVEE116 pKa = 4.46 KK117 pKa = 10.86 SDD119 pKa = 3.61 EE120 pKa = 3.95
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.915
IPC2_protein 4.139
IPC_protein 4.088
Toseland 3.884
ProMoST 4.19
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.91
Grimsley 3.795
Solomon 4.062
Lehninger 4.012
Nozaki 4.19
DTASelect 4.406
Thurlkill 3.935
EMBOSS 3.999
Sillero 4.202
Patrickios 0.744
IPC_peptide 4.062
IPC2_peptide 4.19
IPC2.peptide.svr19 4.128
Protein with the highest isoelectric point:
>tr|A0A2H5BPF4|A0A2H5BPF4_9CAUD Uncharacterized protein OS=Salmonella phage Mutine OX=2054274 GN=CPT_Mutine_081 PE=4 SV=1
MM1 pKa = 7.37 ACKK4 pKa = 9.41 TPEE7 pKa = 3.85 MKK9 pKa = 10.54 LVVSSTGRR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SRR22 pKa = 11.84 SRR24 pKa = 11.84 NLITRR29 pKa = 11.84 LDD31 pKa = 3.63 ILRR34 pKa = 11.84 QKK36 pKa = 9.72 YY37 pKa = 9.03 QGHH40 pKa = 5.49 SVFTRR45 pKa = 11.84 IRR47 pKa = 11.84 NALKK51 pKa = 10.19 EE52 pKa = 3.98 GRR54 pKa = 11.84 TEE56 pKa = 4.12 LEE58 pKa = 3.91 LYY60 pKa = 10.03 RR61 pKa = 11.84 PNGSTRR67 pKa = 11.84 AYY69 pKa = 8.21 QTTDD73 pKa = 2.69 GLLEE77 pKa = 4.85 LIRR80 pKa = 11.84 LSGMSIEE87 pKa = 4.55 PRR89 pKa = 11.84 SSGTPLCSLYY99 pKa = 11.17 VIGNLGALL107 pKa = 3.84
Molecular weight: 12.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.794
IPC_protein 10.804
Toseland 10.716
ProMoST 10.584
Dawson 10.833
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 10.891
Grimsley 10.906
Solomon 11.008
Lehninger 10.965
Nozaki 10.716
DTASelect 10.628
Thurlkill 10.73
EMBOSS 11.14
Sillero 10.774
Patrickios 10.628
IPC_peptide 11.008
IPC2_peptide 9.853
IPC2.peptide.svr19 8.414
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
218
0
218
49971
35
1612
229.2
25.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.458 ± 0.167
1.219 ± 0.08
6.372 ± 0.12
6.592 ± 0.181
4.327 ± 0.095
6.752 ± 0.182
1.943 ± 0.113
6.328 ± 0.103
6.478 ± 0.169
7.975 ± 0.144
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.896 ± 0.098
5.183 ± 0.132
3.878 ± 0.106
3.806 ± 0.106
4.981 ± 0.141
6.45 ± 0.174
5.943 ± 0.215
7.084 ± 0.155
1.441 ± 0.082
3.896 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here