Acinetobacter phage SH-Ab 15497
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5BHK5|A0A2H5BHK5_9CAUD Uncharacterized protein OS=Acinetobacter phage SH-Ab 15497 OX=2060946 GN=SHab15497_00049 PE=4 SV=1
MM1 pKa = 7.43 TFDD4 pKa = 4.15 TNPTCPYY11 pKa = 8.76 CDD13 pKa = 3.44 HH14 pKa = 6.85 VVDD17 pKa = 5.88 SDD19 pKa = 5.4 LADD22 pKa = 3.12 LWEE25 pKa = 4.52 YY26 pKa = 10.8 EE27 pKa = 4.64 GEE29 pKa = 4.28 DD30 pKa = 4.47 NPYY33 pKa = 10.64 EE34 pKa = 4.31 CPNCEE39 pKa = 4.46 KK40 pKa = 10.81 EE41 pKa = 4.91 LILNVQVTTEE51 pKa = 4.18 YY52 pKa = 9.93 EE53 pKa = 4.21 LKK55 pKa = 10.5 RR56 pKa = 11.84 VEE58 pKa = 4.61 CDD60 pKa = 3.67 DD61 pKa = 5.11 DD62 pKa = 3.7 KK63 pKa = 11.79 HH64 pKa = 7.43 EE65 pKa = 4.3 YY66 pKa = 10.74 DD67 pKa = 3.02 EE68 pKa = 4.09 WTRR71 pKa = 11.84 IDD73 pKa = 3.49 VSQRR77 pKa = 11.84 LLDD80 pKa = 4.25 DD81 pKa = 3.74 WAKK84 pKa = 10.83 DD85 pKa = 3.58 PAMSRR90 pKa = 11.84 YY91 pKa = 9.9 ADD93 pKa = 3.84 EE94 pKa = 5.69 KK95 pKa = 8.5 PHH97 pKa = 5.29 SWYY100 pKa = 9.79 IRR102 pKa = 11.84 EE103 pKa = 4.78 CKK105 pKa = 10.35 HH106 pKa = 6.84 CDD108 pKa = 3.35 DD109 pKa = 4.81 KK110 pKa = 11.65 DD111 pKa = 3.89 YY112 pKa = 11.16 SIQYY116 pKa = 9.18 EE117 pKa = 4.29 YY118 pKa = 10.18 QQEE121 pKa = 4.39 VPQDD125 pKa = 4.04 EE126 pKa = 4.47 IKK128 pKa = 10.82 SRR130 pKa = 11.84 YY131 pKa = 9.91 DD132 pKa = 3.07 KK133 pKa = 11.65 DD134 pKa = 3.46 EE135 pKa = 3.88
Molecular weight: 16.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.886
IPC2_protein 4.126
IPC_protein 4.113
Toseland 3.91
ProMoST 4.228
Dawson 4.088
Bjellqvist 4.266
Wikipedia 3.986
Rodwell 3.935
Grimsley 3.821
Solomon 4.075
Lehninger 4.037
Nozaki 4.19
DTASelect 4.406
Thurlkill 3.948
EMBOSS 3.999
Sillero 4.228
Patrickios 0.998
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.115
Protein with the highest isoelectric point:
>tr|A0A2H5BHH2|A0A2H5BHH2_9CAUD Uncharacterized protein OS=Acinetobacter phage SH-Ab 15497 OX=2060946 GN=SHab15497_00004 PE=4 SV=1
MM1 pKa = 6.92 GQWEE5 pKa = 4.18 VDD7 pKa = 3.23 MRR9 pKa = 11.84 RR10 pKa = 11.84 LKK12 pKa = 11.04 GLTEE16 pKa = 3.81 AKK18 pKa = 8.93 MVKK21 pKa = 9.41 VLKK24 pKa = 9.79 IAAMEE29 pKa = 4.08 TMRR32 pKa = 11.84 DD33 pKa = 3.67 VIMDD37 pKa = 4.0 TPVDD41 pKa = 3.54 TGRR44 pKa = 11.84 LRR46 pKa = 11.84 GNWQVGIGQKK56 pKa = 10.47 EE57 pKa = 4.16 SGQLSVLDD65 pKa = 4.18 PNGGITIARR74 pKa = 11.84 ITSKK78 pKa = 10.59 LVQINKK84 pKa = 9.34 NDD86 pKa = 3.97 TIVFYY91 pKa = 11.61 NNLPYY96 pKa = 10.36 AYY98 pKa = 8.86 PIEE101 pKa = 4.83 FGHH104 pKa = 6.21 SAVKK108 pKa = 10.39 APQGMVRR115 pKa = 11.84 KK116 pKa = 10.16 NIANWGGRR124 pKa = 11.84 VARR127 pKa = 11.84 IARR130 pKa = 11.84 VVKK133 pKa = 10.52 NEE135 pKa = 3.74 RR136 pKa = 11.84 AA137 pKa = 3.27
Molecular weight: 15.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 9.472
IPC_protein 9.648
Toseland 10.467
ProMoST 10.014
Dawson 10.57
Bjellqvist 10.175
Wikipedia 10.701
Rodwell 11.023
Grimsley 10.599
Solomon 10.613
Lehninger 10.599
Nozaki 10.423
DTASelect 10.189
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.482
Patrickios 10.774
IPC_peptide 10.628
IPC2_peptide 8.639
IPC2.peptide.svr19 8.672
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58
0
58
13327
42
1018
229.8
25.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.939 ± 0.433
1.148 ± 0.163
6.543 ± 0.221
7.181 ± 0.304
3.489 ± 0.179
7.173 ± 0.286
2.124 ± 0.245
6.19 ± 0.25
6.581 ± 0.365
7.654 ± 0.27
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.129 ± 0.138
4.547 ± 0.178
4.044 ± 0.282
3.797 ± 0.174
5.035 ± 0.196
5.823 ± 0.278
5.823 ± 0.336
6.671 ± 0.284
1.598 ± 0.119
3.512 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here