Streptococcus satellite phage Javan265
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZI31|A0A4D5ZI31_9VIRU XRE family transcriptional regulator OS=Streptococcus satellite phage Javan265 OX=2558597 GN=JavanS265_0003 PE=4 SV=1
MM1 pKa = 7.44 IAYY4 pKa = 9.73 GDD6 pKa = 4.23 TIHH9 pKa = 7.18 EE10 pKa = 4.7 YY11 pKa = 9.5 IDD13 pKa = 3.8 KK14 pKa = 9.55 RR15 pKa = 11.84 TEE17 pKa = 3.8 QLKK20 pKa = 9.5 EE21 pKa = 3.5 QATFEE26 pKa = 4.01 EE27 pKa = 5.51 LINKK31 pKa = 7.76 VALYY35 pKa = 10.26 EE36 pKa = 4.58 SEE38 pKa = 4.11 LLDD41 pKa = 3.57 YY42 pKa = 11.28 AEE44 pKa = 5.23 RR45 pKa = 11.84 LLSDD49 pKa = 3.74 EE50 pKa = 4.79 PLNADD55 pKa = 3.49 SEE57 pKa = 4.84 TAIGTLDD64 pKa = 3.63 MLDD67 pKa = 4.84 DD68 pKa = 3.92 EE69 pKa = 6.32 AIDD72 pKa = 3.89 LFKK75 pKa = 11.26 SVDD78 pKa = 3.58 VNDD81 pKa = 4.21 EE82 pKa = 4.28 YY83 pKa = 11.53 QGLEE87 pKa = 4.1 YY88 pKa = 11.25 YY89 pKa = 8.19 NTSLNKK95 pKa = 9.95 EE96 pKa = 4.21 DD97 pKa = 3.57
Molecular weight: 11.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.966
IPC2_protein 3.834
IPC_protein 3.783
Toseland 3.592
ProMoST 3.884
Dawson 3.757
Bjellqvist 3.961
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.884
DTASelect 4.05
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.897
Patrickios 2.791
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A4D5ZMQ2|A0A4D5ZMQ2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan265 OX=2558597 GN=JavanS265_0011 PE=4 SV=1
MM1 pKa = 7.51 NIKK4 pKa = 10.04 EE5 pKa = 4.3 VIKK8 pKa = 10.68 KK9 pKa = 10.47 DD10 pKa = 3.49 GIKK13 pKa = 10.07 VYY15 pKa = 10.53 RR16 pKa = 11.84 SNVYY20 pKa = 10.53 LGVDD24 pKa = 4.04 SITGKK29 pKa = 10.18 KK30 pKa = 10.14 VKK32 pKa = 8.5 TTVTGRR38 pKa = 11.84 TKK40 pKa = 10.89 KK41 pKa = 9.66 EE42 pKa = 4.03 VKK44 pKa = 9.46 TKK46 pKa = 10.56 AQQAQSNFKK55 pKa = 11.0 ANGSTVFKK63 pKa = 10.15 RR64 pKa = 11.84 VEE66 pKa = 3.73 VTTYY70 pKa = 11.13 KK71 pKa = 10.76 EE72 pKa = 4.39 LTDD75 pKa = 3.82 LWLEE79 pKa = 4.13 NYY81 pKa = 10.11 QMTVKK86 pKa = 10.31 PQTLVNTRR94 pKa = 11.84 QFLRR98 pKa = 11.84 NHH100 pKa = 6.87 ILPVFGDD107 pKa = 3.56 MQLDD111 pKa = 4.24 KK112 pKa = 11.2 IHH114 pKa = 6.9 IAHH117 pKa = 6.2 IQSWVNKK124 pKa = 9.54 LAFKK128 pKa = 10.19 IVNYY132 pKa = 10.05 GVAASINKK140 pKa = 9.97 RR141 pKa = 11.84 ILQYY145 pKa = 10.53 GVSMQLIPFNPARR158 pKa = 11.84 EE159 pKa = 4.31 VILPRR164 pKa = 11.84 PQKK167 pKa = 10.82 AGANRR172 pKa = 11.84 IKK174 pKa = 10.79 FIDD177 pKa = 3.91 KK178 pKa = 10.6 EE179 pKa = 4.13 DD180 pKa = 3.62 LKK182 pKa = 10.6 TFLDD186 pKa = 3.89 YY187 pKa = 10.72 MEE189 pKa = 4.66 QLAPTAYY196 pKa = 10.07 NYY198 pKa = 11.09 YY199 pKa = 9.71 YY200 pKa = 11.02 DD201 pKa = 3.73 SVLYY205 pKa = 10.49 KK206 pKa = 10.87 LLLATGCRR214 pKa = 11.84 YY215 pKa = 10.48 GEE217 pKa = 4.05 AVALEE222 pKa = 4.11 WSDD225 pKa = 3.51 IDD227 pKa = 4.39 FNSATISITKK237 pKa = 8.4 TYY239 pKa = 10.4 NRR241 pKa = 11.84 IVKK244 pKa = 9.79 QVGTPKK250 pKa = 10.34 SKK252 pKa = 10.94 AGIRR256 pKa = 11.84 TISIDD261 pKa = 3.21 NKK263 pKa = 9.16 TILMLKK269 pKa = 9.54 QYY271 pKa = 10.83 RR272 pKa = 11.84 NRR274 pKa = 11.84 QRR276 pKa = 11.84 QAFMEE281 pKa = 4.49 IGSPAPALVFSTTVSQYY298 pKa = 10.05 PNSDD302 pKa = 2.89 ARR304 pKa = 11.84 TKK306 pKa = 10.38 SLRR309 pKa = 11.84 HH310 pKa = 5.33 RR311 pKa = 11.84 CKK313 pKa = 10.52 EE314 pKa = 3.48 AGTPQFTFHH323 pKa = 7.37 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGIGYY340 pKa = 9.74 KK341 pKa = 9.96 EE342 pKa = 3.9 LQHH345 pKa = 6.67 RR346 pKa = 11.84 LGHH349 pKa = 6.08 ATLAMTMDD357 pKa = 4.61 TYY359 pKa = 11.85 SHH361 pKa = 7.05 LSKK364 pKa = 10.62 EE365 pKa = 4.29 KK366 pKa = 9.9 EE367 pKa = 4.1 KK368 pKa = 11.04 EE369 pKa = 3.74 AVLYY373 pKa = 9.73 YY374 pKa = 10.67 EE375 pKa = 5.49 KK376 pKa = 10.92 ALQNLL381 pKa = 3.78
Molecular weight: 43.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.107
IPC2_protein 9.341
IPC_protein 9.268
Toseland 9.955
ProMoST 9.648
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.657
Grimsley 10.248
Solomon 10.189
Lehninger 10.145
Nozaki 9.94
DTASelect 9.823
Thurlkill 10.014
EMBOSS 10.365
Sillero 10.087
Patrickios 10.072
IPC_peptide 10.189
IPC2_peptide 8.361
IPC2.peptide.svr19 8.294
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2215
46
542
158.2
18.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.508 ± 0.637
0.497 ± 0.167
5.418 ± 0.685
8.397 ± 1.206
4.244 ± 0.35
4.966 ± 0.448
1.67 ± 0.24
7.223 ± 0.5
9.345 ± 0.562
10.203 ± 0.585
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.393 ± 0.414
5.869 ± 0.531
3.567 ± 0.494
4.74 ± 0.294
4.108 ± 0.42
5.102 ± 0.427
6.14 ± 0.436
4.876 ± 0.436
0.722 ± 0.145
5.011 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here