Ralstonia phage PE226

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Parhipatevirus; Ralstonia virus PE226

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5F071|E5F071_9VIRU Capsid protein G8P OS=Ralstonia phage PE226 OX=926543 PE=3 SV=1
MM1 pKa = 7.01AQCVQIVGGQFQLDD15 pKa = 3.49STAASSCTGYY25 pKa = 11.29LLLTADD31 pKa = 4.33EE32 pKa = 4.54VTLLHH37 pKa = 7.36AIPPLSIQDD46 pKa = 3.48GATISGAIVGVWAVAWGFRR65 pKa = 11.84AVARR69 pKa = 11.84LFFQRR74 pKa = 11.84DD75 pKa = 3.51EE76 pKa = 4.32EE77 pKa = 4.54VLL79 pKa = 3.66

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5F072|E5F072_9VIRU Putative minor coat protein OS=Ralstonia phage PE226 OX=926543 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.5FGIKK6 pKa = 9.84RR7 pKa = 11.84LAVVAAGVGGAVAAGAANAAIDD29 pKa = 3.92LTAVTTAIGDD39 pKa = 3.43AGTAVGTIGSAVLVVIVGIAVYY61 pKa = 8.59MWVRR65 pKa = 11.84RR66 pKa = 11.84PIKK69 pKa = 10.41

Molecular weight: 6.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1738

60

465

193.1

20.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.623 ± 0.788

2.417 ± 0.835

4.661 ± 0.555

4.143 ± 1.199

3.28 ± 0.526

9.839 ± 0.848

1.611 ± 0.581

4.43 ± 0.736

3.74 ± 0.792

7.883 ± 1.12

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.505

2.417 ± 0.583

5.466 ± 0.961

4.603 ± 0.552

5.581 ± 1.513

6.444 ± 1.627

6.962 ± 1.502

8.458 ± 1.058

1.554 ± 0.292

2.417 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski