Streptococcus phage SOCP
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4MYX3|A0A0B4MYX3_BPCP1 DNA-directed DNA polymerase OS=Streptococcus phage SOCP OX=1498213 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.12 MKK4 pKa = 10.54 ALYY7 pKa = 10.24 VNHH10 pKa = 6.73 NDD12 pKa = 3.07 EE13 pKa = 4.43 TFAEE17 pKa = 4.58 LTHH20 pKa = 6.2 GQALDD25 pKa = 3.79 VIFNADD31 pKa = 3.4 NAEE34 pKa = 4.09 MYY36 pKa = 10.46 GDD38 pKa = 4.05 HH39 pKa = 6.79 VNQIVDD45 pKa = 4.22 LGNEE49 pKa = 4.16 IKK51 pKa = 11.04 LEE53 pKa = 4.01 LYY55 pKa = 10.51 RR56 pKa = 11.84 NNEE59 pKa = 4.35 YY60 pKa = 10.52 IPFAGYY66 pKa = 7.91 YY67 pKa = 9.74 EE68 pKa = 4.25 RR69 pKa = 11.84 VKK71 pKa = 10.12 TLSEE75 pKa = 4.58 LYY77 pKa = 10.45 TNISDD82 pKa = 3.21 WFEE85 pKa = 3.78 EE86 pKa = 4.57 VEE88 pKa = 4.1
Molecular weight: 10.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.074
IPC2_protein 4.393
IPC_protein 4.266
Toseland 4.126
ProMoST 4.317
Dawson 4.215
Bjellqvist 4.431
Wikipedia 4.088
Rodwell 4.113
Grimsley 4.037
Solomon 4.215
Lehninger 4.164
Nozaki 4.329
DTASelect 4.457
Thurlkill 4.139
EMBOSS 4.101
Sillero 4.38
Patrickios 1.977
IPC_peptide 4.215
IPC2_peptide 4.38
IPC2.peptide.svr19 4.295
Protein with the highest isoelectric point:
>tr|A0A0B4MZ86|A0A0B4MZ86_BPCP1 Putative scaffolding protein OS=Streptococcus phage SOCP OX=1498213 PE=4 SV=1
MM1 pKa = 7.75 ALTPKK6 pKa = 9.43 QRR8 pKa = 11.84 KK9 pKa = 6.56 VRR11 pKa = 11.84 RR12 pKa = 11.84 DD13 pKa = 3.05 YY14 pKa = 10.56 LTRR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.67 RR20 pKa = 11.84 TLQQQGASNAEE31 pKa = 3.69 IKK33 pKa = 10.93 AFMGGRR39 pKa = 11.84 WNFAGMSDD47 pKa = 3.47 KK48 pKa = 11.02 ALEE51 pKa = 3.98 RR52 pKa = 11.84 AYY54 pKa = 10.76 DD55 pKa = 3.92 EE56 pKa = 4.65 IKK58 pKa = 10.93 SKK60 pKa = 10.79 GRR62 pKa = 11.84 TQVFGNHH69 pKa = 6.14 VYY71 pKa = 10.32 TSDD74 pKa = 3.59 YY75 pKa = 10.42 VKK77 pKa = 10.49 KK78 pKa = 10.6 AKK80 pKa = 9.77 AWYY83 pKa = 8.37 GDD85 pKa = 3.34 KK86 pKa = 10.93 FSVEE90 pKa = 4.17 KK91 pKa = 9.67 LTQGFRR97 pKa = 11.84 SSQRR101 pKa = 11.84 SEE103 pKa = 3.86 LNRR106 pKa = 11.84 FHH108 pKa = 7.13 SVKK111 pKa = 9.89 EE112 pKa = 3.98 VKK114 pKa = 9.73 EE115 pKa = 4.03 YY116 pKa = 10.41 RR117 pKa = 11.84 SEE119 pKa = 3.9 RR120 pKa = 11.84 DD121 pKa = 2.97 RR122 pKa = 11.84 EE123 pKa = 4.12 AKK125 pKa = 9.46 EE126 pKa = 4.46 RR127 pKa = 11.84 YY128 pKa = 8.62 IQALEE133 pKa = 3.85 EE134 pKa = 3.84 MHH136 pKa = 6.81 YY137 pKa = 9.12 NTRR140 pKa = 11.84 DD141 pKa = 3.39 AGNKK145 pKa = 8.51 AQEE148 pKa = 4.25 KK149 pKa = 9.58 AFKK152 pKa = 11.04 SMISRR157 pKa = 11.84 IRR159 pKa = 11.84 RR160 pKa = 11.84 MSASNFGAFLTGGASDD176 pKa = 4.45 KK177 pKa = 11.23 VSFDD181 pKa = 3.17 NVMVFVDD188 pKa = 3.69 TDD190 pKa = 3.45 GKK192 pKa = 8.64 EE193 pKa = 4.08 TAFEE197 pKa = 4.23 FQDD200 pKa = 3.39 SLARR204 pKa = 11.84 EE205 pKa = 3.86 ILDD208 pKa = 4.0 NVDD211 pKa = 3.49 KK212 pKa = 10.92 FSKK215 pKa = 10.57 QFVSDD220 pKa = 2.91 MRR222 pKa = 11.84 RR223 pKa = 11.84 RR224 pKa = 11.84 KK225 pKa = 9.98 KK226 pKa = 10.27 RR227 pKa = 11.84 GKK229 pKa = 9.86 KK230 pKa = 9.43
Molecular weight: 26.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.126
IPC2_protein 9.399
IPC_protein 9.428
Toseland 10.335
ProMoST 9.911
Dawson 10.452
Bjellqvist 10.058
Wikipedia 10.584
Rodwell 10.979
Grimsley 10.496
Solomon 10.482
Lehninger 10.467
Nozaki 10.292
DTASelect 10.058
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.365
Patrickios 10.672
IPC_peptide 10.496
IPC2_peptide 8.463
IPC2.peptide.svr19 8.533
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27
0
27
5922
80
612
219.3
25.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.113 ± 0.42
0.659 ± 0.127
6.113 ± 0.263
7.345 ± 0.37
5.522 ± 0.42
5.657 ± 0.508
1.469 ± 0.207
6.991 ± 0.341
7.717 ± 0.532
8.038 ± 0.506
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.161 ± 0.199
7.227 ± 0.44
2.499 ± 0.294
3.985 ± 0.293
3.715 ± 0.42
6.299 ± 0.41
6.147 ± 0.442
5.623 ± 0.319
1.317 ± 0.269
5.404 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here