Cowpox virus (strain Brighton Red) (CPV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Cowpox virus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QN23|Q8QN23_CWPXB CPXV039 OS=Cowpox virus (strain Brighton Red) OX=265872 GN=CPXV039 CDS PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 4.42NSMLSMFMYY11 pKa = 10.8NNIVDD16 pKa = 4.15YY17 pKa = 11.5VDD19 pKa = 5.79DD20 pKa = 5.16DD21 pKa = 4.42VDD23 pKa = 4.9DD24 pKa = 6.14GIDD27 pKa = 3.4NGIVPDD33 pKa = 3.97IDD35 pKa = 3.5NGIVPDD41 pKa = 3.97IDD43 pKa = 3.5NGIVPDD49 pKa = 4.3IEE51 pKa = 6.13DD52 pKa = 3.77EE53 pKa = 4.59ASDD56 pKa = 4.34NDD58 pKa = 3.59DD59 pKa = 3.53HH60 pKa = 8.44NYY62 pKa = 10.24VYY64 pKa = 10.27PLPEE68 pKa = 3.83NMVYY72 pKa = 10.53RR73 pKa = 11.84FDD75 pKa = 3.76KK76 pKa = 10.0STNILDD82 pKa = 3.9YY83 pKa = 11.21LSTEE87 pKa = 4.1RR88 pKa = 11.84DD89 pKa = 3.57HH90 pKa = 10.24VMMAVQYY97 pKa = 10.31YY98 pKa = 8.63MSKK101 pKa = 10.26QRR103 pKa = 11.84LDD105 pKa = 3.35DD106 pKa = 4.85LYY108 pKa = 11.09RR109 pKa = 11.84QLPTKK114 pKa = 8.67TRR116 pKa = 11.84SYY118 pKa = 10.88IDD120 pKa = 4.43IINMYY125 pKa = 9.31CDD127 pKa = 3.99KK128 pKa = 11.33VNNDD132 pKa = 3.47YY133 pKa = 11.25NRR135 pKa = 11.84DD136 pKa = 3.51MNIMYY141 pKa = 10.73DD142 pKa = 3.5MASTEE147 pKa = 4.21SFTVYY152 pKa = 10.63DD153 pKa = 3.93INNEE157 pKa = 3.96VNTILIDD164 pKa = 3.78NKK166 pKa = 10.86GLGVRR171 pKa = 11.84LATISFITEE180 pKa = 4.16LGRR183 pKa = 11.84RR184 pKa = 11.84CMNPVEE190 pKa = 4.32TIKK193 pKa = 10.52MFTLLSHH200 pKa = 6.92TICDD204 pKa = 3.94DD205 pKa = 3.66CFVDD209 pKa = 4.46YY210 pKa = 9.46ITDD213 pKa = 3.68ISPPDD218 pKa = 3.31NTIPNTSTRR227 pKa = 11.84EE228 pKa = 3.86YY229 pKa = 10.49LKK231 pKa = 10.99LIGITAIMFATYY243 pKa = 7.95KK244 pKa = 8.27TLKK247 pKa = 10.56YY248 pKa = 10.15MIGG251 pKa = 3.33

Molecular weight: 29.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q85306|Q85306_CWPXB CPXV002 protein OS=Cowpox virus (strain Brighton Red) OX=265872 GN=CPXV228 CDS PE=4 SV=1
MM1 pKa = 7.17NHH3 pKa = 5.73WQHH6 pKa = 6.23PFIVQKK12 pKa = 11.1APIYY16 pKa = 9.37KK17 pKa = 9.79RR18 pKa = 11.84KK19 pKa = 10.14SRR21 pKa = 11.84FDD23 pKa = 3.44RR24 pKa = 11.84DD25 pKa = 3.35KK26 pKa = 11.22QLTILSLFLTPPQFLISLVMKK47 pKa = 10.17LFTISVSSLSTRR59 pKa = 11.84DD60 pKa = 3.38FTNLITLAVSFNRR73 pKa = 11.84VVIFVCVILISFF85 pKa = 4.71

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

225

1

226

68474

50

1919

303.0

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.831 ± 0.108

2.176 ± 0.096

6.883 ± 0.116

5.404 ± 0.179

4.403 ± 0.132

3.958 ± 0.116

2.142 ± 0.076

9.463 ± 0.209

7.207 ± 0.113

8.751 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.763 ± 0.08

6.914 ± 0.137

3.394 ± 0.104

2.172 ± 0.076

4.384 ± 0.158

7.802 ± 0.144

6.093 ± 0.144

6.121 ± 0.114

0.681 ± 0.04

5.46 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski