Elderberry carlavirus E

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; Sambucus virus E

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7M929|A0A0A7M929_9VIRU Helicase OS=Elderberry carlavirus E OX=1569056 PE=3 SV=2
MM1 pKa = 8.07DD2 pKa = 6.14IIIEE6 pKa = 4.06LAEE9 pKa = 3.82QYY11 pKa = 10.93GFQRR15 pKa = 11.84TKK17 pKa = 10.98SKK19 pKa = 10.52LVKK22 pKa = 9.82PLVFHH27 pKa = 6.6CVPGAGKK34 pKa = 8.48STFIRR39 pKa = 11.84LLLAEE44 pKa = 4.6IPGSKK49 pKa = 10.49AFTHH53 pKa = 6.62GVADD57 pKa = 3.91TPTICGLYY65 pKa = 9.95IRR67 pKa = 11.84DD68 pKa = 3.9AKK70 pKa = 10.94DD71 pKa = 2.94IEE73 pKa = 4.48LTSSGSALLLDD84 pKa = 4.88EE85 pKa = 4.17YY86 pKa = 11.11TEE88 pKa = 4.39FEE90 pKa = 4.44EE91 pKa = 6.15LPTHH95 pKa = 6.05TLAAFGDD102 pKa = 4.07PLQSNKK108 pKa = 9.83YY109 pKa = 9.9APRR112 pKa = 11.84EE113 pKa = 3.66PHH115 pKa = 7.02FISDD119 pKa = 3.71LTRR122 pKa = 11.84RR123 pKa = 11.84FGNQTVLLLKK133 pKa = 10.72SLGFNVRR140 pKa = 11.84TEE142 pKa = 4.02KK143 pKa = 10.8VDD145 pKa = 3.55TVVVEE150 pKa = 4.82DD151 pKa = 4.56IYY153 pKa = 11.51AGEE156 pKa = 4.21PEE158 pKa = 4.26GQILCNEE165 pKa = 4.24RR166 pKa = 11.84EE167 pKa = 4.3VAALLCAHH175 pKa = 6.14QLDD178 pKa = 4.59YY179 pKa = 11.73LFVDD183 pKa = 3.65QLRR186 pKa = 11.84GRR188 pKa = 11.84TFDD191 pKa = 2.89KK192 pKa = 10.02VTYY195 pKa = 8.63ITAEE199 pKa = 3.86NSPSDD204 pKa = 3.26RR205 pKa = 11.84VGSFQCLTRR214 pKa = 11.84HH215 pKa = 4.43TTKK218 pKa = 10.9LKK220 pKa = 10.33ILCPDD225 pKa = 3.18ATYY228 pKa = 11.02GPAEE232 pKa = 4.18LFF234 pKa = 3.75

Molecular weight: 26.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4KJC8|A0A0U4KJC8_9VIRU Capsid protein OS=Elderberry carlavirus E OX=1569056 PE=3 SV=1
MM1 pKa = 7.33GRR3 pKa = 11.84DD4 pKa = 3.15RR5 pKa = 11.84LTTLFLTIQACDD17 pKa = 3.12AFIPMDD23 pKa = 3.8LCVYY27 pKa = 9.93ILSKK31 pKa = 10.74SKK33 pKa = 10.72PPLCTGGSSTYY44 pKa = 10.13ARR46 pKa = 11.84KK47 pKa = 9.68RR48 pKa = 11.84RR49 pKa = 11.84ARR51 pKa = 11.84KK52 pKa = 9.15IGRR55 pKa = 11.84CWRR58 pKa = 11.84CFRR61 pKa = 11.84VSPPIFSSKK70 pKa = 10.74CNGSTCEE77 pKa = 4.47PGISYY82 pKa = 10.0NWRR85 pKa = 11.84VAEE88 pKa = 4.43FINRR92 pKa = 11.84GVTEE96 pKa = 4.41VIPRR100 pKa = 11.84MM101 pKa = 3.8

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2782

74

1958

463.7

51.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.231 ± 0.561

2.48 ± 0.726

4.817 ± 0.572

6.398 ± 1.139

4.745 ± 0.521

6.075 ± 0.408

2.876 ± 0.298

6.003 ± 0.556

5.931 ± 0.814

10.712 ± 1.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.338

3.595 ± 0.406

5.14 ± 0.897

2.372 ± 0.262

5.967 ± 0.687

7.009 ± 0.566

6.039 ± 0.567

5.284 ± 0.423

0.971 ± 0.17

3.487 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski