Capybara microvirus Cap1_SP_119
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W471|A0A4P8W471_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_119 OX=2584811 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 9.77 PVNYY6 pKa = 9.86 FNRR9 pKa = 11.84 DD10 pKa = 3.28 CITPKK15 pKa = 10.55 NEE17 pKa = 3.98 EE18 pKa = 5.07 LICQSKK24 pKa = 10.62 DD25 pKa = 2.74 GKK27 pKa = 9.12 TFFAPFPSVVAQAKK41 pKa = 10.03 DD42 pKa = 3.51 YY43 pKa = 11.27 SLEE46 pKa = 4.04 SLLAANIDD54 pKa = 3.6 PSKK57 pKa = 11.04 LVIKK61 pKa = 9.83 VKK63 pKa = 10.84 KK64 pKa = 10.48 EE65 pKa = 3.6 GVTDD69 pKa = 4.32 FDD71 pKa = 3.97 EE72 pKa = 4.28 EE73 pKa = 4.84 TIVVRR78 pKa = 11.84 LNSIVEE84 pKa = 4.17 NLDD87 pKa = 3.31 EE88 pKa = 4.71 SNQPNEE94 pKa = 4.11 SS95 pKa = 3.32
Molecular weight: 10.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.739
IPC2_protein 4.711
IPC_protein 4.558
Toseland 4.393
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.368
Rodwell 4.38
Grimsley 4.304
Solomon 4.482
Lehninger 4.444
Nozaki 4.609
DTASelect 4.749
Thurlkill 4.393
EMBOSS 4.38
Sillero 4.66
Patrickios 4.151
IPC_peptide 4.495
IPC2_peptide 4.647
IPC2.peptide.svr19 4.642
Protein with the highest isoelectric point:
>tr|A0A4P8W7V1|A0A4P8W7V1_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_119 OX=2584811 PE=3 SV=1
MM1 pKa = 7.98 KK2 pKa = 9.94 IRR4 pKa = 11.84 NKK6 pKa = 9.43 STFYY10 pKa = 10.78 SDD12 pKa = 3.42 YY13 pKa = 10.97 SKK15 pKa = 8.89 THH17 pKa = 6.29 FDD19 pKa = 3.08 IDD21 pKa = 2.92 TDD23 pKa = 3.61 IFVRR27 pKa = 11.84 SCSSVYY33 pKa = 10.34 SYY35 pKa = 10.94 SVRR38 pKa = 11.84 NYY40 pKa = 9.66 YY41 pKa = 9.79 EE42 pKa = 4.03 YY43 pKa = 11.27 KK44 pKa = 10.59 SVIDD48 pKa = 4.06 SGGQCFFYY56 pKa = 10.63 TLTHH60 pKa = 6.56 NDD62 pKa = 2.97 SSIVRR67 pKa = 11.84 LYY69 pKa = 10.92 GRR71 pKa = 11.84 SFPNYY76 pKa = 8.29 EE77 pKa = 3.88 GLRR80 pKa = 11.84 NLVNGAFKK88 pKa = 10.7 KK89 pKa = 10.48 RR90 pKa = 11.84 IEE92 pKa = 4.15 KK93 pKa = 8.86 LTNCRR98 pKa = 11.84 FTYY101 pKa = 10.09 FAVTEE106 pKa = 4.3 FGEE109 pKa = 4.67 GKK111 pKa = 10.45 GYY113 pKa = 10.57 RR114 pKa = 11.84 GLDD117 pKa = 3.62 NNPHH121 pKa = 4.44 YY122 pKa = 10.87 HH123 pKa = 6.03 ILFYY127 pKa = 10.31 LHH129 pKa = 7.39 PCTEE133 pKa = 4.1 KK134 pKa = 10.83 GSYY137 pKa = 10.22 VPISSNDD144 pKa = 2.84 FCEE147 pKa = 3.81 LVRR150 pKa = 11.84 YY151 pKa = 8.07 YY152 pKa = 9.92 WQGDD156 pKa = 3.32 KK157 pKa = 11.13 YY158 pKa = 10.47 HH159 pKa = 6.73 YY160 pKa = 10.15 KK161 pKa = 10.65 DD162 pKa = 3.58 SLHH165 pKa = 7.26 ILEE168 pKa = 5.13 PKK170 pKa = 10.38 DD171 pKa = 3.75 YY172 pKa = 9.8 IYY174 pKa = 11.1 GIASPSNINNGEE186 pKa = 4.01 VTNFSAIRR194 pKa = 11.84 YY195 pKa = 5.91 CCKK198 pKa = 10.65 YY199 pKa = 8.18 ITKK202 pKa = 10.32 DD203 pKa = 2.38 SYY205 pKa = 11.22 YY206 pKa = 10.48 QVFRR210 pKa = 11.84 KK211 pKa = 9.97 NLLKK215 pKa = 10.47 DD216 pKa = 3.51 IYY218 pKa = 10.9 YY219 pKa = 10.57 SIVKK223 pKa = 9.97 QLTLCDD229 pKa = 5.33 DD230 pKa = 3.98 IDD232 pKa = 3.77 IQTDD236 pKa = 3.52 PNYY239 pKa = 9.4 VTSRR243 pKa = 11.84 LISFCGSLNTAFDD256 pKa = 5.4 FINEE260 pKa = 4.1 QQKK263 pKa = 10.71 KK264 pKa = 9.07 IYY266 pKa = 9.49 CSLYY270 pKa = 9.44 SYY272 pKa = 10.5 IHH274 pKa = 6.64 LVHH277 pKa = 6.78 CSKK280 pKa = 11.16 KK281 pKa = 10.46 LGSLGLEE288 pKa = 4.27 YY289 pKa = 9.6 IDD291 pKa = 4.8 YY292 pKa = 10.89 DD293 pKa = 4.15 NNSLTIPFPDD303 pKa = 3.11 SKK305 pKa = 11.37 GSLRR309 pKa = 11.84 QCSLPLYY316 pKa = 9.46 YY317 pKa = 10.05 YY318 pKa = 10.67 RR319 pKa = 11.84 KK320 pKa = 8.78 VCYY323 pKa = 10.02 DD324 pKa = 2.67 ITKK327 pKa = 9.39 NYY329 pKa = 9.27 RR330 pKa = 11.84 GNVHH334 pKa = 6.6 YY335 pKa = 10.41 YY336 pKa = 10.83 LNDD339 pKa = 3.35 YY340 pKa = 10.26 GIKK343 pKa = 10.11 CKK345 pKa = 10.93 SNLFPKK351 pKa = 10.22 DD352 pKa = 3.35 LKK354 pKa = 11.08 KK355 pKa = 10.83 QFDD358 pKa = 3.59 ICKK361 pKa = 8.58 STISSYY367 pKa = 11.17 FDD369 pKa = 5.2 DD370 pKa = 6.36 DD371 pKa = 5.83 LLTDD375 pKa = 3.83 YY376 pKa = 11.64 NNLHH380 pKa = 6.61 INSSFTYY387 pKa = 10.56 QDD389 pKa = 3.06 IKK391 pKa = 11.31 DD392 pKa = 4.1 LYY394 pKa = 11.24 HH395 pKa = 7.45 NDD397 pKa = 3.67 EE398 pKa = 4.59 LLNAYY403 pKa = 9.68 VLFNFVYY410 pKa = 10.37 RR411 pKa = 11.84 EE412 pKa = 3.55 RR413 pKa = 11.84 FYY415 pKa = 11.37 DD416 pKa = 3.34 RR417 pKa = 11.84 RR418 pKa = 11.84 HH419 pKa = 5.77 ISICPEE425 pKa = 3.21 FDD427 pKa = 3.44 YY428 pKa = 11.51 EE429 pKa = 4.33 HH430 pKa = 6.93 FLVPSLVEE438 pKa = 3.97 TPFTHH443 pKa = 7.54 IEE445 pKa = 4.1 PNLSKK450 pKa = 11.27 YY451 pKa = 9.96 EE452 pKa = 4.14 SYY454 pKa = 11.38 EE455 pKa = 3.63 NNPYY459 pKa = 10.11 FKK461 pKa = 10.42 PYY463 pKa = 8.84 MPKK466 pKa = 10.45 FSLLDD471 pKa = 3.4 EE472 pKa = 5.0 LFTFFKK478 pKa = 9.94 KK479 pKa = 8.61 TAQEE483 pKa = 4.09 YY484 pKa = 10.16 KK485 pKa = 9.01 IKK487 pKa = 10.21 EE488 pKa = 4.03 YY489 pKa = 10.58 EE490 pKa = 3.65 RR491 pKa = 11.84 RR492 pKa = 11.84 KK493 pKa = 8.88 YY494 pKa = 10.47 LKK496 pKa = 9.98 RR497 pKa = 11.84 VHH499 pKa = 5.66 STQNII504 pKa = 3.46
Molecular weight: 60.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.063
IPC2_protein 7.38
IPC_protein 7.205
Toseland 6.898
ProMoST 7.775
Dawson 7.892
Bjellqvist 8.258
Wikipedia 7.805
Rodwell 7.907
Grimsley 6.971
Solomon 7.922
Lehninger 7.936
Nozaki 8.331
DTASelect 8.024
Thurlkill 8.053
EMBOSS 8.097
Sillero 8.361
Patrickios 0.998
IPC_peptide 7.922
IPC2_peptide 7.366
IPC2.peptide.svr19 7.384
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1633
95
583
326.6
37.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.735 ± 0.885
1.715 ± 0.642
5.94 ± 0.587
5.266 ± 0.647
6.185 ± 0.625
4.838 ± 0.816
1.776 ± 0.538
6.491 ± 0.252
6.552 ± 0.678
8.757 ± 0.572
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.653 ± 0.571
7.655 ± 0.307
3.429 ± 0.411
3.184 ± 0.587
4.287 ± 0.368
10.533 ± 0.882
4.654 ± 0.354
6.062 ± 0.711
0.367 ± 0.114
6.92 ± 1.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here