Shigella virus 2019SD1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Hanrivervirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5C9S1|A0A6M5C9S1_9CAUD Uncharacterized protein OS=Shigella virus 2019SD1 OX=2736192 GN=SD1_15 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 3.83SCIYY6 pKa = 10.8EE7 pKa = 4.72DD8 pKa = 4.26GDD10 pKa = 3.86PVNVVSIGNVWCLTSEE26 pKa = 4.36SEE28 pKa = 4.24APEE31 pKa = 4.21YY32 pKa = 10.36GDD34 pKa = 4.19KK35 pKa = 11.39VFVTPDD41 pKa = 2.84GDD43 pKa = 3.47ASYY46 pKa = 11.48SEE48 pKa = 4.78GDD50 pKa = 4.04LLSGWFFTGDD60 pKa = 2.98HH61 pKa = 6.75CGDD64 pKa = 3.57EE65 pKa = 5.37FIAPGKK71 pKa = 9.8IVRR74 pKa = 11.84LSHH77 pKa = 5.55ITNGYY82 pKa = 9.54KK83 pKa = 9.71IASVNGGLVIGVAVRR98 pKa = 11.84PPDD101 pKa = 3.34SCIYY105 pKa = 10.75EE106 pKa = 4.68DD107 pKa = 4.3GDD109 pKa = 3.86PVNVVSIGNVWCLTSEE125 pKa = 4.36SEE127 pKa = 4.24APEE130 pKa = 4.21YY131 pKa = 10.36GDD133 pKa = 4.19KK134 pKa = 11.39VFVTPDD140 pKa = 2.84GDD142 pKa = 3.47ASYY145 pKa = 11.48SEE147 pKa = 4.78GDD149 pKa = 4.04LLSGWFFTGDD159 pKa = 3.7HH160 pKa = 6.77IKK162 pKa = 9.66TGPDD166 pKa = 3.04SYY168 pKa = 11.25IVGVSFSAA176 pKa = 3.79

Molecular weight: 18.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5C9X4|A0A6M5C9X4_9CAUD Terminase small subunit OS=Shigella virus 2019SD1 OX=2736192 GN=SD1_76 PE=4 SV=1
MM1 pKa = 7.56PRR3 pKa = 11.84YY4 pKa = 9.07NANTNQRR11 pKa = 11.84EE12 pKa = 4.38GLNMLEE18 pKa = 3.91LHH20 pKa = 7.24KK21 pKa = 10.7IRR23 pKa = 11.84VGTKK27 pKa = 8.16FVVTRR32 pKa = 11.84VCGEE36 pKa = 3.58MCGIRR41 pKa = 11.84KK42 pKa = 9.98GEE44 pKa = 3.98IVTASDD50 pKa = 3.89RR51 pKa = 11.84YY52 pKa = 9.99IGSTKK57 pKa = 10.05EE58 pKa = 3.69FNRR61 pKa = 11.84PRR63 pKa = 11.84IKK65 pKa = 10.36NGYY68 pKa = 9.12IITRR72 pKa = 11.84RR73 pKa = 11.84LGFDD77 pKa = 3.72HH78 pKa = 6.74YY79 pKa = 11.16CVVATQGVLIEE90 pKa = 4.44LKK92 pKa = 10.31RR93 pKa = 11.84ISDD96 pKa = 3.71HH97 pKa = 6.52RR98 pKa = 11.84GCHH101 pKa = 5.23IKK103 pKa = 9.68TMKK106 pKa = 10.33TPFQRR111 pKa = 11.84ARR113 pKa = 11.84YY114 pKa = 7.91DD115 pKa = 3.15ARR117 pKa = 11.84RR118 pKa = 11.84MRR120 pKa = 11.84RR121 pKa = 11.84LARR124 pKa = 11.84NAIKK128 pKa = 10.27FKK130 pKa = 10.43KK131 pKa = 9.62HH132 pKa = 4.75GSNFYY137 pKa = 9.33RR138 pKa = 11.84TYY140 pKa = 11.05KK141 pKa = 10.74GIARR145 pKa = 11.84NDD147 pKa = 3.28GKK149 pKa = 11.04

Molecular weight: 17.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

14591

37

1202

192.0

21.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.957 ± 0.349

1.357 ± 0.155

5.826 ± 0.253

6.011 ± 0.205

3.804 ± 0.172

7.004 ± 0.248

1.967 ± 0.211

6.511 ± 0.205

7.052 ± 0.316

7.326 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.31 ± 0.236

4.695 ± 0.251

3.345 ± 0.19

4.085 ± 0.304

6.031 ± 0.296

6.593 ± 0.248

5.846 ± 0.295

6.463 ± 0.217

1.48 ± 0.124

3.338 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski