Paenibacillus wynnii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4821 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A098M988|A0A098M988_9BACL Alpha/beta hydrolase OS=Paenibacillus wynnii OX=268407 GN=PWYN_04010 PE=4 SV=1
MM1 pKa = 7.49NSIGPQGAQGFPGEE15 pKa = 4.96HH16 pKa = 6.86GSQGAPGNQGLRR28 pKa = 11.84GPQGSQGLPGAAGPQGSRR46 pKa = 11.84GLPGAAGPQGSQGLPGAAGPQGSQGLPGAAGPQGSQGLPGAAGPQGSQGLPGAAGPQGSQGLPGPTGPAGPTGPAGSSPQINSTLTADD134 pKa = 3.89RR135 pKa = 11.84YY136 pKa = 10.51FYY138 pKa = 10.45TADD141 pKa = 3.65SDD143 pKa = 3.54ISPPMDD149 pKa = 3.15IPANQFTNDD158 pKa = 3.46NGDD161 pKa = 3.97SITEE165 pKa = 4.34FPALDD170 pKa = 3.55QNSYY174 pKa = 11.81ADD176 pKa = 3.95LFINGIMQVGGTYY189 pKa = 10.07SVSPSTLNLLNNQDD203 pKa = 4.15TIFTGTPLSLEE214 pKa = 4.24FVQLLVQVIQQ224 pKa = 3.55

Molecular weight: 21.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A098MBR5|A0A098MBR5_9BACL Uncharacterized protein OS=Paenibacillus wynnii OX=268407 GN=PWYN_12100 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.56GRR39 pKa = 11.84KK40 pKa = 8.92SLSAA44 pKa = 3.86

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4821

0

4821

1490243

26

2211

309.1

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.552 ± 0.047

0.781 ± 0.01

5.107 ± 0.025

6.812 ± 0.036

4.172 ± 0.028

7.379 ± 0.033

2.048 ± 0.02

7.125 ± 0.036

5.613 ± 0.036

10.264 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.016

4.147 ± 0.029

3.877 ± 0.022

3.689 ± 0.022

4.753 ± 0.031

6.597 ± 0.024

5.473 ± 0.025

6.979 ± 0.029

1.227 ± 0.017

3.552 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski