Amino acid dipepetide frequency for Carnobacterium sp. CP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.012AlaAla: 7.012 ± 0.134
0.452AlaCys: 0.452 ± 0.023
3.993AlaAsp: 3.993 ± 0.098
5.561AlaGlu: 5.561 ± 0.115
3.413AlaPhe: 3.413 ± 0.077
5.672AlaGly: 5.672 ± 0.092
1.144AlaHis: 1.144 ± 0.042
6.362AlaIle: 6.362 ± 0.101
5.074AlaLys: 5.074 ± 0.099
7.672AlaLeu: 7.672 ± 0.123
2.051AlaMet: 2.051 ± 0.057
3.07AlaAsn: 3.07 ± 0.069
2.05AlaPro: 2.05 ± 0.051
2.492AlaGln: 2.492 ± 0.065
2.313AlaArg: 2.313 ± 0.061
4.269AlaSer: 4.269 ± 0.08
4.189AlaThr: 4.189 ± 0.082
5.77AlaVal: 5.77 ± 0.116
0.562AlaTrp: 0.562 ± 0.033
2.589AlaTyr: 2.589 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.327CysAla: 0.327 ± 0.023
0.055CysCys: 0.055 ± 0.009
0.27CysAsp: 0.27 ± 0.02
0.312CysGlu: 0.312 ± 0.021
0.287CysPhe: 0.287 ± 0.021
0.506CysGly: 0.506 ± 0.028
0.138CysHis: 0.138 ± 0.015
0.364CysIle: 0.364 ± 0.023
0.215CysLys: 0.215 ± 0.015
0.58CysLeu: 0.58 ± 0.027
0.123CysMet: 0.123 ± 0.014
0.208CysAsn: 0.208 ± 0.019
0.225CysPro: 0.225 ± 0.016
0.213CysGln: 0.213 ± 0.02
0.222CysArg: 0.222 ± 0.017
0.358CysSer: 0.358 ± 0.021
0.271CysThr: 0.271 ± 0.019
0.299CysVal: 0.299 ± 0.024
0.042CysTrp: 0.042 ± 0.007
0.186CysTyr: 0.186 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.813AspAla: 3.813 ± 0.083
0.284AspCys: 0.284 ± 0.021
2.483AspAsp: 2.483 ± 0.065
4.35AspGlu: 4.35 ± 0.09
2.701AspPhe: 2.701 ± 0.067
3.367AspGly: 3.367 ± 0.074
1.051AspHis: 1.051 ± 0.043
4.278AspIle: 4.278 ± 0.081
3.335AspLys: 3.335 ± 0.074
5.14AspLeu: 5.14 ± 0.089
1.429AspMet: 1.429 ± 0.047
2.005AspAsn: 2.005 ± 0.05
2.05AspPro: 2.05 ± 0.057
1.999AspGln: 1.999 ± 0.052
1.934AspArg: 1.934 ± 0.052
2.924AspSer: 2.924 ± 0.078
3.063AspThr: 3.063 ± 0.074
3.687AspVal: 3.687 ± 0.083
0.54AspTrp: 0.54 ± 0.028
2.549AspTyr: 2.549 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
6.204GluAla: 6.204 ± 0.112
0.306GluCys: 0.306 ± 0.027
4.044GluAsp: 4.044 ± 0.097
6.977GluGlu: 6.977 ± 0.117
2.348GluPhe: 2.348 ± 0.058
4.118GluGly: 4.118 ± 0.079
1.216GluHis: 1.216 ± 0.044
5.612GluIle: 5.612 ± 0.099
6.773GluLys: 6.773 ± 0.109
7.398GluLeu: 7.398 ± 0.106
2.417GluMet: 2.417 ± 0.063
4.112GluAsn: 4.112 ± 0.085
2.138GluPro: 2.138 ± 0.053
3.416GluGln: 3.416 ± 0.082
3.012GluArg: 3.012 ± 0.067
3.674GluSer: 3.674 ± 0.075
4.971GluThr: 4.971 ± 0.07
5.217GluVal: 5.217 ± 0.096
0.696GluTrp: 0.696 ± 0.034
2.139GluTyr: 2.139 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
2.841PheAla: 2.841 ± 0.068
0.268PheCys: 0.268 ± 0.019
2.56PheAsp: 2.56 ± 0.065
2.84PheGlu: 2.84 ± 0.065
2.259PhePhe: 2.259 ± 0.077
3.321PheGly: 3.321 ± 0.079
0.759PheHis: 0.759 ± 0.034
3.719PheIle: 3.719 ± 0.102
2.532PheLys: 2.532 ± 0.057
4.262PheLeu: 4.262 ± 0.099
1.141PheMet: 1.141 ± 0.046
2.064PheAsn: 2.064 ± 0.052
1.503PhePro: 1.503 ± 0.046
1.454PheGln: 1.454 ± 0.05
1.274PheArg: 1.274 ± 0.045
3.414PheSer: 3.414 ± 0.077
2.534PheThr: 2.534 ± 0.054
3.083PheVal: 3.083 ± 0.069
0.453PheTrp: 0.453 ± 0.026
1.707PheTyr: 1.707 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.765GlyAla: 4.765 ± 0.093
0.41GlyCys: 0.41 ± 0.026
3.184GlyAsp: 3.184 ± 0.073
4.49GlyGlu: 4.49 ± 0.097
3.268GlyPhe: 3.268 ± 0.071
4.755GlyGly: 4.755 ± 0.097
1.274GlyHis: 1.274 ± 0.042
6.047GlyIle: 6.047 ± 0.095
4.892GlyLys: 4.892 ± 0.084
6.704GlyLeu: 6.704 ± 0.113
2.058GlyMet: 2.058 ± 0.056
2.812GlyAsn: 2.812 ± 0.064
1.49GlyPro: 1.49 ± 0.049
2.338GlyGln: 2.338 ± 0.06
2.341GlyArg: 2.341 ± 0.062
4.122GlySer: 4.122 ± 0.082
4.536GlyThr: 4.536 ± 0.079
4.93GlyVal: 4.93 ± 0.094
0.661GlyTrp: 0.661 ± 0.037
2.803GlyTyr: 2.803 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.201HisAla: 1.201 ± 0.04
0.124HisCys: 0.124 ± 0.012
0.853HisAsp: 0.853 ± 0.035
1.247HisGlu: 1.247 ± 0.041
0.935HisPhe: 0.935 ± 0.041
1.12HisGly: 1.12 ± 0.043
0.491HisHis: 0.491 ± 0.028
1.197HisIle: 1.197 ± 0.04
0.899HisLys: 0.899 ± 0.035
1.938HisLeu: 1.938 ± 0.058
0.447HisMet: 0.447 ± 0.024
0.635HisAsn: 0.635 ± 0.032
1.036HisPro: 1.036 ± 0.037
0.838HisGln: 0.838 ± 0.037
0.738HisArg: 0.738 ± 0.036
1.05HisSer: 1.05 ± 0.038
0.98HisThr: 0.98 ± 0.043
1.032HisVal: 1.032 ± 0.039
0.182HisTrp: 0.182 ± 0.015
0.898HisTyr: 0.898 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
6.183IleAla: 6.183 ± 0.102
0.604IleCys: 0.604 ± 0.027
4.601IleAsp: 4.601 ± 0.088
6.105IleGlu: 6.105 ± 0.101
3.027IlePhe: 3.027 ± 0.077
6.1IleGly: 6.1 ± 0.11
1.369IleHis: 1.369 ± 0.046
5.436IleIle: 5.436 ± 0.113
4.855IleLys: 4.855 ± 0.079
7.192IleLeu: 7.192 ± 0.131
1.79IleMet: 1.79 ± 0.052
3.192IleAsn: 3.192 ± 0.071
3.192IlePro: 3.192 ± 0.072
3.131IleGln: 3.131 ± 0.073
2.876IleArg: 2.876 ± 0.066
4.716IleSer: 4.716 ± 0.082
4.281IleThr: 4.281 ± 0.076
5.477IleVal: 5.477 ± 0.079
0.543IleTrp: 0.543 ± 0.029
2.434IleTyr: 2.434 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
5.082LysAla: 5.082 ± 0.116
0.204LysCys: 0.204 ± 0.018
3.74LysAsp: 3.74 ± 0.081
7.227LysGlu: 7.227 ± 0.107
1.753LysPhe: 1.753 ± 0.051
4.36LysGly: 4.36 ± 0.09
1.0LysHis: 1.0 ± 0.039
4.828LysIle: 4.828 ± 0.084
6.479LysLys: 6.479 ± 0.114
5.422LysLeu: 5.422 ± 0.088
2.357LysMet: 2.357 ± 0.056
3.804LysAsn: 3.804 ± 0.081
2.135LysPro: 2.135 ± 0.052
3.009LysGln: 3.009 ± 0.078
3.01LysArg: 3.01 ± 0.066
3.367LysSer: 3.367 ± 0.065
4.242LysThr: 4.242 ± 0.087
4.357LysVal: 4.357 ± 0.081
0.678LysTrp: 0.678 ± 0.033
1.902LysTyr: 1.902 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
7.989LeuAla: 7.989 ± 0.102
0.453LeuCys: 0.453 ± 0.025
5.017LeuAsp: 5.017 ± 0.087
6.794LeuGlu: 6.794 ± 0.107
4.73LeuPhe: 4.73 ± 0.103
6.233LeuGly: 6.233 ± 0.105
1.621LeuHis: 1.621 ± 0.047
7.264LeuIle: 7.264 ± 0.119
6.711LeuLys: 6.711 ± 0.1
9.852LeuLeu: 9.852 ± 0.172
2.701LeuMet: 2.701 ± 0.066
4.79LeuAsn: 4.79 ± 0.084
3.751LeuPro: 3.751 ± 0.069
3.23LeuGln: 3.23 ± 0.067
3.175LeuArg: 3.175 ± 0.068
6.956LeuSer: 6.956 ± 0.106
6.454LeuThr: 6.454 ± 0.088
6.609LeuVal: 6.609 ± 0.112
0.698LeuTrp: 0.698 ± 0.033
2.97LeuTyr: 2.97 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.138MetAla: 2.138 ± 0.054
0.102MetCys: 0.102 ± 0.013
1.63MetAsp: 1.63 ± 0.049
1.927MetGlu: 1.927 ± 0.06
0.845MetPhe: 0.845 ± 0.038
1.872MetGly: 1.872 ± 0.046
0.421MetHis: 0.421 ± 0.021
2.143MetIle: 2.143 ± 0.057
2.264MetLys: 2.264 ± 0.056
2.388MetLeu: 2.388 ± 0.059
0.884MetMet: 0.884 ± 0.039
1.607MetAsn: 1.607 ± 0.049
1.007MetPro: 1.007 ± 0.038
1.04MetGln: 1.04 ± 0.041
0.966MetArg: 0.966 ± 0.036
1.78MetSer: 1.78 ± 0.048
1.758MetThr: 1.758 ± 0.057
2.016MetVal: 2.016 ± 0.047
0.148MetTrp: 0.148 ± 0.014
0.734MetTyr: 0.734 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
2.992AsnAla: 2.992 ± 0.058
0.253AsnCys: 0.253 ± 0.018
2.392AsnAsp: 2.392 ± 0.061
3.677AsnGlu: 3.677 ± 0.071
1.784AsnPhe: 1.784 ± 0.05
3.349AsnGly: 3.349 ± 0.083
1.044AsnHis: 1.044 ± 0.04
3.181AsnIle: 3.181 ± 0.078
3.008AsnLys: 3.008 ± 0.064
4.153AsnLeu: 4.153 ± 0.093
1.216AsnMet: 1.216 ± 0.042
1.974AsnAsn: 1.974 ± 0.059
2.036AsnPro: 2.036 ± 0.056
2.352AsnGln: 2.352 ± 0.065
1.924AsnArg: 1.924 ± 0.056
2.378AsnSer: 2.378 ± 0.07
2.275AsnThr: 2.275 ± 0.059
2.999AsnVal: 2.999 ± 0.063
0.491AsnTrp: 0.491 ± 0.026
1.797AsnTyr: 1.797 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
2.609ProAla: 2.609 ± 0.063
0.155ProCys: 0.155 ± 0.015
1.934ProAsp: 1.934 ± 0.049
3.117ProGlu: 3.117 ± 0.063
1.827ProPhe: 1.827 ± 0.06
2.083ProGly: 2.083 ± 0.059
0.657ProHis: 0.657 ± 0.031
2.808ProIle: 2.808 ± 0.062
2.101ProLys: 2.101 ± 0.05
3.385ProLeu: 3.385 ± 0.078
0.829ProMet: 0.829 ± 0.035
1.563ProAsn: 1.563 ± 0.039
0.663ProPro: 0.663 ± 0.03
1.112ProGln: 1.112 ± 0.04
0.888ProArg: 0.888 ± 0.032
1.953ProSer: 1.953 ± 0.061
2.127ProThr: 2.127 ± 0.051
2.692ProVal: 2.692 ± 0.067
0.277ProTrp: 0.277 ± 0.019
1.394ProTyr: 1.394 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
3.07GlnAla: 3.07 ± 0.076
0.115GlnCys: 0.115 ± 0.012
1.784GlnAsp: 1.784 ± 0.048
3.089GlnGlu: 3.089 ± 0.078
1.657GlnPhe: 1.657 ± 0.051
2.092GlnGly: 2.092 ± 0.05
0.657GlnHis: 0.657 ± 0.031
2.645GlnIle: 2.645 ± 0.07
2.954GlnLys: 2.954 ± 0.074
4.367GlnLeu: 4.367 ± 0.089
1.216GlnMet: 1.216 ± 0.049
1.692GlnAsn: 1.692 ± 0.052
1.279GlnPro: 1.279 ± 0.046
1.92GlnGln: 1.92 ± 0.069
1.337GlnArg: 1.337 ± 0.042
2.034GlnSer: 2.034 ± 0.06
2.535GlnThr: 2.535 ± 0.062
2.431GlnVal: 2.431 ± 0.068
0.366GlnTrp: 0.366 ± 0.025
1.317GlnTyr: 1.317 ± 0.045
0.0GlnXaa: 0.0 ± 0.0
Arg
2.162ArgAla: 2.162 ± 0.053
0.164ArgCys: 0.164 ± 0.016
1.77ArgAsp: 1.77 ± 0.054
2.669ArgGlu: 2.669 ± 0.069
1.784ArgPhe: 1.784 ± 0.048
2.019ArgGly: 2.019 ± 0.06
0.695ArgHis: 0.695 ± 0.033
2.882ArgIle: 2.882 ± 0.063
2.756ArgLys: 2.756 ± 0.061
3.577ArgLeu: 3.577 ± 0.08
1.194ArgMet: 1.194 ± 0.043
1.744ArgAsn: 1.744 ± 0.049
1.166ArgPro: 1.166 ± 0.047
1.327ArgGln: 1.327 ± 0.046
1.495ArgArg: 1.495 ± 0.047
2.011ArgSer: 2.011 ± 0.056
2.032ArgThr: 2.032 ± 0.051
2.489ArgVal: 2.489 ± 0.057
0.289ArgTrp: 0.289 ± 0.021
1.59ArgTyr: 1.59 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
4.165SerAla: 4.165 ± 0.094
0.26SerCys: 0.26 ± 0.015
3.08SerAsp: 3.08 ± 0.066
4.378SerGlu: 4.378 ± 0.079
3.08SerPhe: 3.08 ± 0.085
4.615SerGly: 4.615 ± 0.082
1.054SerHis: 1.054 ± 0.043
4.902SerIle: 4.902 ± 0.076
3.714SerLys: 3.714 ± 0.081
6.028SerLeu: 6.028 ± 0.098
1.538SerMet: 1.538 ± 0.044
2.605SerAsn: 2.605 ± 0.066
1.799SerPro: 1.799 ± 0.048
2.138SerGln: 2.138 ± 0.053
2.185SerArg: 2.185 ± 0.057
4.189SerSer: 4.189 ± 0.121
3.375SerThr: 3.375 ± 0.083
4.104SerVal: 4.104 ± 0.087
0.529SerTrp: 0.529 ± 0.03
2.258SerTyr: 2.258 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
4.803ThrAla: 4.803 ± 0.089
0.261ThrCys: 0.261 ± 0.023
3.269ThrAsp: 3.269 ± 0.064
4.204ThrGlu: 4.204 ± 0.081
2.71ThrPhe: 2.71 ± 0.057
4.463ThrGly: 4.463 ± 0.08
1.149ThrHis: 1.149 ± 0.042
5.245ThrIle: 5.245 ± 0.085
3.8ThrLys: 3.8 ± 0.084
5.913ThrLeu: 5.913 ± 0.082
1.404ThrMet: 1.404 ± 0.047
2.665ThrAsn: 2.665 ± 0.064
2.39ThrPro: 2.39 ± 0.065
1.939ThrGln: 1.939 ± 0.048
1.83ThrArg: 1.83 ± 0.047
3.507ThrSer: 3.507 ± 0.078
3.614ThrThr: 3.614 ± 0.087
4.694ThrVal: 4.694 ± 0.09
0.484ThrTrp: 0.484 ± 0.029
2.037ThrTyr: 2.037 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
5.44ValAla: 5.44 ± 0.113
0.449ValCys: 0.449 ± 0.025
3.796ValAsp: 3.796 ± 0.079
4.894ValGlu: 4.894 ± 0.087
3.227ValPhe: 3.227 ± 0.079
4.764ValGly: 4.764 ± 0.087
1.216ValHis: 1.216 ± 0.047
5.303ValIle: 5.303 ± 0.097
4.169ValLys: 4.169 ± 0.072
7.135ValLeu: 7.135 ± 0.107
1.909ValMet: 1.909 ± 0.052
2.827ValAsn: 2.827 ± 0.061
2.624ValPro: 2.624 ± 0.063
2.327ValGln: 2.327 ± 0.059
2.394ValArg: 2.394 ± 0.066
4.694ValSer: 4.694 ± 0.087
4.527ValThr: 4.527 ± 0.081
5.267ValVal: 5.267 ± 0.099
0.565ValTrp: 0.565 ± 0.035
2.293ValTyr: 2.293 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
0.53TrpAla: 0.53 ± 0.028
0.042TrpCys: 0.042 ± 0.008
0.498TrpAsp: 0.498 ± 0.033
0.579TrpGlu: 0.579 ± 0.03
0.45TrpPhe: 0.45 ± 0.027
0.586TrpGly: 0.586 ± 0.031
0.159TrpHis: 0.159 ± 0.014
0.664TrpIle: 0.664 ± 0.039
0.612TrpLys: 0.612 ± 0.028
0.951TrpLeu: 0.951 ± 0.04
0.245TrpMet: 0.245 ± 0.017
0.474TrpAsn: 0.474 ± 0.03
0.225TrpPro: 0.225 ± 0.017
0.352TrpGln: 0.352 ± 0.021
0.341TrpArg: 0.341 ± 0.021
0.508TrpSer: 0.508 ± 0.03
0.501TrpThr: 0.501 ± 0.026
0.516TrpVal: 0.516 ± 0.038
0.096TrpTrp: 0.096 ± 0.011
0.268TrpTyr: 0.268 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.383TyrAla: 2.383 ± 0.053
0.24TyrCys: 0.24 ± 0.019
2.083TyrAsp: 2.083 ± 0.056
2.353TyrGlu: 2.353 ± 0.064
1.903TyrPhe: 1.903 ± 0.053
2.485TyrGly: 2.485 ± 0.056
0.724TyrHis: 0.724 ± 0.035
2.223TyrIle: 2.223 ± 0.051
1.813TyrLys: 1.813 ± 0.055
3.943TyrLeu: 3.943 ± 0.079
0.717TyrMet: 0.717 ± 0.035
1.432TyrAsn: 1.432 ± 0.046
1.414TyrPro: 1.414 ± 0.048
1.934TyrGln: 1.934 ± 0.058
1.559TyrArg: 1.559 ± 0.049
2.093TyrSer: 2.093 ± 0.057
2.117TyrThr: 2.117 ± 0.049
2.152TyrVal: 2.152 ± 0.057
0.298TyrTrp: 0.298 ± 0.02
1.401TyrTyr: 1.401 ± 0.048
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2388 proteins (715207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski