Influenza A virus (A/Uruguay/716/2007(H3N2))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H3N2 subtype

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2ZV39|B2ZV39_9INFA Matrix protein 1 OS=Influenza A virus (A/Uruguay/716/2007(H3N2)) OX=529058 GN=M1 PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.17VEE8 pKa = 4.27TPIRR12 pKa = 11.84NEE14 pKa = 3.51WGCRR18 pKa = 11.84CNDD21 pKa = 3.04SSDD24 pKa = 3.96PLVVAANIIGILHH37 pKa = 7.21LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.47CVYY52 pKa = 10.28RR53 pKa = 11.84LFKK56 pKa = 10.69HH57 pKa = 5.21GLKK60 pKa = 10.25RR61 pKa = 11.84GPSTEE66 pKa = 4.02GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.96EE75 pKa = 4.11YY76 pKa = 10.39RR77 pKa = 11.84KK78 pKa = 9.21EE79 pKa = 3.68QQNAVDD85 pKa = 4.6ADD87 pKa = 3.82DD88 pKa = 3.72SHH90 pKa = 6.77FVSIEE95 pKa = 3.83LEE97 pKa = 3.99

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2ZV35|B2ZV35_9INFA Isoform of B2ZV32 Hemagglutinin OS=Influenza A virus (A/Uruguay/716/2007(H3N2)) OX=529058 GN=HA PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.03VEE8 pKa = 4.57TYY10 pKa = 10.48VLSIVPSGPLKK21 pKa = 11.07AEE23 pKa = 3.62IAQRR27 pKa = 11.84LEE29 pKa = 3.96DD30 pKa = 3.83VFAGKK35 pKa = 8.33NTDD38 pKa = 4.04LEE40 pKa = 5.12ALMEE44 pKa = 4.21WLKK47 pKa = 9.79TRR49 pKa = 11.84PILSPLTKK57 pKa = 10.4GILGFVFTLTVPSEE71 pKa = 4.2RR72 pKa = 11.84GLQRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84FVQNALNGNGDD89 pKa = 4.29PNNMDD94 pKa = 3.97KK95 pKa = 10.95AVKK98 pKa = 10.21LYY100 pKa = 10.69RR101 pKa = 11.84KK102 pKa = 9.56LKK104 pKa = 10.61RR105 pKa = 11.84EE106 pKa = 3.99ITFHH110 pKa = 5.58GAKK113 pKa = 9.88EE114 pKa = 3.75IALSYY119 pKa = 10.62SAGALASCMGLIYY132 pKa = 10.91NRR134 pKa = 11.84MGAVTTEE141 pKa = 3.97VAFGLVCATCEE152 pKa = 3.92QIADD156 pKa = 3.85SQHH159 pKa = 6.13RR160 pKa = 11.84SHH162 pKa = 6.75RR163 pKa = 11.84QMVATTNPLIRR174 pKa = 11.84HH175 pKa = 5.69EE176 pKa = 4.07NRR178 pKa = 11.84MVLASTTAKK187 pKa = 10.72AMEE190 pKa = 4.01QMAGSSEE197 pKa = 4.03QAAEE201 pKa = 3.98AMEE204 pKa = 4.52IASQARR210 pKa = 11.84QMVQAMRR217 pKa = 11.84AIGTHH222 pKa = 6.45PSSSTGLRR230 pKa = 11.84DD231 pKa = 4.08DD232 pKa = 4.97LLEE235 pKa = 4.61NLQTYY240 pKa = 6.98QKK242 pKa = 10.99RR243 pKa = 11.84MGVQMQRR250 pKa = 11.84FKK252 pKa = 11.48

Molecular weight: 27.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

2

6

2419

97

566

403.2

45.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.919 ± 0.879

3.473 ± 0.401

5.085 ± 0.288

5.581 ± 0.393

3.597 ± 0.207

7.482 ± 0.258

2.108 ± 0.094

8.144 ± 0.511

5.622 ± 0.318

7.193 ± 0.697

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.471

6.78 ± 0.611

3.762 ± 0.123

3.679 ± 0.463

4.961 ± 0.342

8.268 ± 0.616

6.697 ± 0.412

5.788 ± 0.617

1.943 ± 0.213

3.018 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski