Psychrobacillus psychrotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5WDG9|A0A1I5WDG9_9BACI Flagellar biosynthetic protein FliP OS=Psychrobacillus psychrotolerans OX=126156 GN=fliP PE=3 SV=1
MM1 pKa = 7.32VGTILSNFWVALIAFTIYY19 pKa = 10.64FLFSYY24 pKa = 9.87PFIEE28 pKa = 4.57GMGIILEE35 pKa = 4.1ACIISFGFFLLTFLARR51 pKa = 11.84AIISYY56 pKa = 10.08IISNPQEE63 pKa = 4.37EE64 pKa = 4.93IEE66 pKa = 5.29DD67 pKa = 3.62IPIADD72 pKa = 4.26DD73 pKa = 3.96AGILKK78 pKa = 10.32SEE80 pKa = 4.76ISPEE84 pKa = 3.99DD85 pKa = 3.38YY86 pKa = 11.41AEE88 pKa = 4.13LVKK91 pKa = 10.4TMLEE95 pKa = 3.91EE96 pKa = 4.3DD97 pKa = 4.12EE98 pKa = 4.82KK99 pKa = 11.44PNTII103 pKa = 4.38

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6AJF4|A0A1I6AJF4_9BACI ATP-dependent DNA helicase OS=Psychrobacillus psychrotolerans OX=126156 GN=SAMN05421670_3392 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.67RR12 pKa = 11.84AKK14 pKa = 9.79NHH16 pKa = 4.64GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.71NGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3550

0

3550

1022668

29

1769

288.1

32.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.935 ± 0.047

0.612 ± 0.01

5.025 ± 0.029

7.568 ± 0.049

4.626 ± 0.033

6.674 ± 0.041

1.955 ± 0.018

8.314 ± 0.04

6.785 ± 0.036

9.805 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.02

4.552 ± 0.027

3.451 ± 0.024

3.684 ± 0.029

3.654 ± 0.029

6.221 ± 0.028

5.726 ± 0.031

7.145 ± 0.033

0.981 ± 0.014

3.478 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski