Tomato leaf curl Bangalore virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q88557|Q88557_9GEMI Replication-associated protein OS=Tomato leaf curl Bangalore virus OX=220608 GN=C1 PE=3 SV=1
MM1 pKa = 8.05WDD3 pKa = 3.72PLTNDD8 pKa = 3.09FPEE11 pKa = 4.61TVHH14 pKa = 6.99GFRR17 pKa = 11.84CMLAIKK23 pKa = 9.86YY24 pKa = 8.69LQLVEE29 pKa = 4.21NTYY32 pKa = 11.21SPDD35 pKa = 3.24SLGYY39 pKa = 10.57DD40 pKa = 4.59LIRR43 pKa = 11.84DD44 pKa = 4.62LISVVRR50 pKa = 11.84AKK52 pKa = 10.74SYY54 pKa = 9.06VQASGRR60 pKa = 11.84YY61 pKa = 7.64DD62 pKa = 3.19HH63 pKa = 6.99FRR65 pKa = 11.84ARR67 pKa = 11.84LEE69 pKa = 3.89VSPTSEE75 pKa = 4.25LNQPIQQACCCPHH88 pKa = 6.69CPRR91 pKa = 11.84HH92 pKa = 5.95KK93 pKa = 10.43GKK95 pKa = 10.89GMGQQAHH102 pKa = 6.0EE103 pKa = 4.6SEE105 pKa = 4.32AHH107 pKa = 5.46VLQDD111 pKa = 3.34VQRR114 pKa = 11.84SS115 pKa = 3.48

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q88556|Q88556_9GEMI Protein V2 OS=Tomato leaf curl Bangalore virus OX=220608 GN=V2 PE=3 SV=1
MM1 pKa = 7.63SKK3 pKa = 10.36RR4 pKa = 11.84PADD7 pKa = 3.94MIISGPVSKK16 pKa = 10.61YY17 pKa = 10.1RR18 pKa = 11.84RR19 pKa = 11.84LLSSISPYY27 pKa = 10.18SKK29 pKa = 9.56RR30 pKa = 11.84AAVRR34 pKa = 11.84IVRR37 pKa = 11.84GTKK40 pKa = 9.48GKK42 pKa = 9.49EE43 pKa = 3.43WANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 8.95PMFYY56 pKa = 10.6RR57 pKa = 11.84MFRR60 pKa = 11.84GPDD63 pKa = 3.25VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.03SRR80 pKa = 11.84HH81 pKa = 5.99DD82 pKa = 3.73IIHH85 pKa = 6.65IGKK88 pKa = 9.23VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.16KK148 pKa = 10.35PQDD151 pKa = 3.67FGDD154 pKa = 3.83VFNMFDD160 pKa = 4.34NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 9.98EE195 pKa = 3.79QALVKK200 pKa = 10.58EE201 pKa = 4.4FVKK204 pKa = 10.84VNNYY208 pKa = 7.92VVYY211 pKa = 10.11NQQEE215 pKa = 3.8AGKK218 pKa = 10.08YY219 pKa = 8.01EE220 pKa = 4.07NHH222 pKa = 6.29SEE224 pKa = 4.11NALMLYY230 pKa = 7.58MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1096

97

360

182.7

20.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.657 ± 0.745

2.007 ± 0.309

5.018 ± 0.596

4.38 ± 0.252

4.836 ± 0.452

5.292 ± 0.517

4.288 ± 0.616

5.383 ± 0.798

5.931 ± 0.714

6.934 ± 0.759

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.776

5.657 ± 0.555

5.474 ± 0.428

5.109 ± 0.732

6.204 ± 0.695

8.85 ± 1.187

5.201 ± 0.418

6.569 ± 0.968

1.46 ± 0.159

3.376 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski