Red clover powdery mildew-associated totivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S3Q2B6|A0A0S3Q2B6_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 1 OX=1714362 GN=CP PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 6.35KK3 pKa = 10.37IIKK6 pKa = 9.92HH7 pKa = 4.71VFEE10 pKa = 4.59SEE12 pKa = 3.85GMAGMPIKK20 pKa = 10.68HH21 pKa = 6.3EE22 pKa = 4.42SGNYY26 pKa = 6.65NTLLKK31 pKa = 10.99GNLAVDD37 pKa = 3.59VNGDD41 pKa = 3.49KK42 pKa = 10.84NIKK45 pKa = 9.1KK46 pKa = 8.67LTMAQSMTYY55 pKa = 10.1LGRR58 pKa = 11.84STVVMGSGSSDD69 pKa = 3.34LEE71 pKa = 4.6GINKK75 pKa = 9.2FCLTPEE81 pKa = 4.26GNLNIVNVLATVAKK95 pKa = 9.97EE96 pKa = 3.91SGMPLNEE103 pKa = 3.76QHH105 pKa = 7.15HH106 pKa = 6.3KK107 pKa = 10.43LEE109 pKa = 4.17YY110 pKa = 8.84MKK112 pKa = 10.84TMSWADD118 pKa = 3.24NHH120 pKa = 6.71VSLLVNLMRR129 pKa = 11.84FAILLEE135 pKa = 4.23LSEE138 pKa = 4.49SSGGVGLKK146 pKa = 10.45GALPKK151 pKa = 11.16YY152 pKa = 9.51NDD154 pKa = 3.16GVVAVNRR161 pKa = 11.84TALLPEE167 pKa = 4.97EE168 pKa = 4.49YY169 pKa = 9.72PVGGDD174 pKa = 3.24MTWFTRR180 pKa = 11.84FSRR183 pKa = 11.84QDD185 pKa = 3.08MPDD188 pKa = 3.09VVRR191 pKa = 11.84VEE193 pKa = 4.89EE194 pKa = 4.38YY195 pKa = 10.34CAPQSDD201 pKa = 4.1VCLDD205 pKa = 3.6LRR207 pKa = 11.84SLTEE211 pKa = 3.91TEE213 pKa = 3.89AVIVLLCCGKK223 pKa = 9.65WYY225 pKa = 9.87RR226 pKa = 11.84QSRR229 pKa = 11.84FLLDD233 pKa = 3.85FDD235 pKa = 5.52CPILTSKK242 pKa = 10.74LYY244 pKa = 10.36YY245 pKa = 10.51RR246 pKa = 11.84FDD248 pKa = 3.28KK249 pKa = 11.08RR250 pKa = 11.84IDD252 pKa = 3.26IGNFDD257 pKa = 3.55QTSDD261 pKa = 3.82GRR263 pKa = 11.84VTQPKK268 pKa = 9.83LPKK271 pKa = 10.27AKK273 pKa = 9.97EE274 pKa = 3.83VWRR277 pKa = 11.84TMSRR281 pKa = 11.84YY282 pKa = 8.96LAANRR287 pKa = 11.84LYY289 pKa = 10.67GQYY292 pKa = 8.97STALCLVAQTMCQFIPATAEE312 pKa = 3.9GNVWLTQQRR321 pKa = 11.84VIHH324 pKa = 6.4LPALKK329 pKa = 9.82SVRR332 pKa = 11.84GALPFINEE340 pKa = 4.44DD341 pKa = 3.42EE342 pKa = 4.46PALVSQRR349 pKa = 11.84SLNEE353 pKa = 3.22WGAFSTDD360 pKa = 2.95VTKK363 pKa = 11.37YY364 pKa = 10.68NLLGNILVQAVQTGIAVRR382 pKa = 11.84ALRR385 pKa = 11.84FRR387 pKa = 11.84NEE389 pKa = 4.35DD390 pKa = 3.46NPEE393 pKa = 3.93DD394 pKa = 4.13LYY396 pKa = 10.63RR397 pKa = 11.84TYY399 pKa = 10.96DD400 pKa = 3.35TVTNPAVMYY409 pKa = 9.99GALCSEE415 pKa = 5.07ALRR418 pKa = 11.84CPVPLAGMSGVFVEE432 pKa = 6.18FEE434 pKa = 4.16LPDD437 pKa = 3.33EE438 pKa = 4.42RR439 pKa = 11.84EE440 pKa = 4.04EE441 pKa = 4.33VEE443 pKa = 5.07LEE445 pKa = 4.21FPVLVYY451 pKa = 10.5DD452 pKa = 4.61PKK454 pKa = 10.85RR455 pKa = 11.84CGSTIEE461 pKa = 3.92KK462 pKa = 10.51RR463 pKa = 11.84RR464 pKa = 11.84DD465 pKa = 3.22TEE467 pKa = 4.06YY468 pKa = 11.12LQLNSIPMAGVPTLLLPLNPIGKK491 pKa = 8.65MSPFNLRR498 pKa = 11.84GVFDD502 pKa = 3.65TADD505 pKa = 3.6LKK507 pKa = 9.1RR508 pKa = 11.84TKK510 pKa = 10.04MGWEE514 pKa = 3.86TDD516 pKa = 4.06LYY518 pKa = 10.99SAWSWAWLCRR528 pKa = 11.84LCGYY532 pKa = 9.55DD533 pKa = 2.77IQMRR537 pKa = 11.84QQYY540 pKa = 8.79GQSCGSQGDD549 pKa = 4.1YY550 pKa = 11.25SPDD553 pKa = 3.93ASWTWPILGSVGHH566 pKa = 6.34NGIVCISKK574 pKa = 10.32LVEE577 pKa = 4.35RR578 pKa = 11.84GNSFIDD584 pKa = 4.19LPPLHH589 pKa = 7.11SKK591 pKa = 9.84SCHH594 pKa = 4.99SVINYY599 pKa = 8.54KK600 pKa = 10.46FSINSHH606 pKa = 4.99SVSLPEE612 pKa = 3.81RR613 pKa = 11.84RR614 pKa = 11.84QPEE617 pKa = 4.74LIGDD621 pKa = 4.47RR622 pKa = 11.84YY623 pKa = 10.56SAAPVLNLRR632 pKa = 11.84HH633 pKa = 6.15INIATTTEE641 pKa = 3.92IIKK644 pKa = 10.76LKK646 pKa = 10.81GFIEE650 pKa = 4.45RR651 pKa = 11.84SEE653 pKa = 4.26SDD655 pKa = 3.48FRR657 pKa = 11.84FADD660 pKa = 3.98DD661 pKa = 3.69VQAGQIPPPEE671 pKa = 4.34VPHH674 pKa = 5.95VASDD678 pKa = 3.72VIEE681 pKa = 4.09

Molecular weight:
76.28 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S3Q2B6|A0A0S3Q2B6_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 1 OX=1714362 GN=CP PE=4 SV=1
MM1 pKa = 7.35WPVFTRR7 pKa = 11.84MSEE10 pKa = 3.84NRR12 pKa = 11.84LEE14 pKa = 4.06VVDD17 pKa = 3.76RR18 pKa = 11.84HH19 pKa = 5.93KK20 pKa = 10.51CTHH23 pKa = 5.51VLVGVEE29 pKa = 4.44EE30 pKa = 5.03DD31 pKa = 3.53TMTWQGNDD39 pKa = 2.33IMYY42 pKa = 10.43AGQEE46 pKa = 4.29CVRR49 pKa = 11.84TCSYY53 pKa = 11.55GDD55 pKa = 3.31GGTRR59 pKa = 11.84AHH61 pKa = 6.44YY62 pKa = 10.53IKK64 pKa = 10.18MNKK67 pKa = 8.66PVSLLPPIVKK77 pKa = 9.36QRR79 pKa = 11.84LSAAYY84 pKa = 10.12SMVGGYY90 pKa = 10.38VYY92 pKa = 11.01GDD94 pKa = 3.54KK95 pKa = 11.27DD96 pKa = 3.29EE97 pKa = 4.67LASVRR102 pKa = 11.84QILKK106 pKa = 9.66PLKK109 pKa = 9.55QNYY112 pKa = 8.55KK113 pKa = 9.77HH114 pKa = 6.65DD115 pKa = 3.94VKK117 pKa = 11.0FSIRR121 pKa = 11.84SLTYY125 pKa = 9.96KK126 pKa = 10.53KK127 pKa = 10.5SATTGEE133 pKa = 4.3HH134 pKa = 5.44HH135 pKa = 5.79THH137 pKa = 4.78YY138 pKa = 10.21TPMEE142 pKa = 4.15VYY144 pKa = 10.89NIVTCDD150 pKa = 2.83ARR152 pKa = 11.84RR153 pKa = 11.84RR154 pKa = 11.84QLVLSMNARR163 pKa = 11.84LRR165 pKa = 11.84QMHH168 pKa = 7.4GITEE172 pKa = 4.53AFAATILMYY181 pKa = 10.39IATARR186 pKa = 11.84EE187 pKa = 4.18SVAVMIATSSTLWARR202 pKa = 11.84NVDD205 pKa = 3.91LTLLRR210 pKa = 11.84LKK212 pKa = 10.26EE213 pKa = 3.92CSVPFKK219 pKa = 10.6SLHH222 pKa = 5.93HH223 pKa = 7.14ADD225 pKa = 5.26LIDD228 pKa = 3.67LTEE231 pKa = 3.99MFEE234 pKa = 4.76LLSLANRR241 pKa = 11.84GYY243 pKa = 11.2GAVDD247 pKa = 3.0WRR249 pKa = 11.84AEE251 pKa = 4.02KK252 pKa = 10.02EE253 pKa = 4.04HH254 pKa = 6.09RR255 pKa = 11.84TKK257 pKa = 10.83VDD259 pKa = 3.99DD260 pKa = 4.36IKK262 pKa = 11.5VKK264 pKa = 10.83SIDD267 pKa = 3.61VYY269 pKa = 10.74RR270 pKa = 11.84SAMKK274 pKa = 10.13IFEE277 pKa = 4.41MGNKK281 pKa = 9.85NGFRR285 pKa = 11.84YY286 pKa = 9.71KK287 pKa = 10.44KK288 pKa = 10.62LSIKK292 pKa = 10.7DD293 pKa = 3.46FTASRR298 pKa = 11.84WEE300 pKa = 3.82WAPAGSVHH308 pKa = 6.1SQHH311 pKa = 6.62EE312 pKa = 4.18DD313 pKa = 2.94DD314 pKa = 5.55RR315 pKa = 11.84VYY317 pKa = 10.69IDD319 pKa = 3.46PSNYY323 pKa = 7.85RR324 pKa = 11.84HH325 pKa = 5.19RR326 pKa = 11.84TKK328 pKa = 10.6FVTLNSMPARR338 pKa = 11.84HH339 pKa = 6.4IEE341 pKa = 4.13TFLEE345 pKa = 4.28RR346 pKa = 11.84EE347 pKa = 4.35PEE349 pKa = 3.87IQAWPSEE356 pKa = 4.03KK357 pKa = 10.11IEE359 pKa = 3.83WGKK362 pKa = 10.31RR363 pKa = 11.84RR364 pKa = 11.84AIYY367 pKa = 10.16GVDD370 pKa = 3.45LTSATLAHH378 pKa = 6.82LAMYY382 pKa = 10.16NCEE385 pKa = 3.78DD386 pKa = 3.68VFRR389 pKa = 11.84HH390 pKa = 5.87RR391 pKa = 11.84FPVGKK396 pKa = 9.25EE397 pKa = 3.73AEE399 pKa = 3.9ASRR402 pKa = 11.84VHH404 pKa = 5.79KK405 pKa = 10.15RR406 pKa = 11.84LRR408 pKa = 11.84AMCEE412 pKa = 3.92GEE414 pKa = 4.02EE415 pKa = 4.27TFCYY419 pKa = 10.82DD420 pKa = 4.02FDD422 pKa = 5.24DD423 pKa = 5.71FNAQHH428 pKa = 6.05STDD431 pKa = 3.25AMIAVLCAYY440 pKa = 9.48RR441 pKa = 11.84DD442 pKa = 3.51KK443 pKa = 11.38YY444 pKa = 9.67AADD447 pKa = 3.31MSAVQRR453 pKa = 11.84EE454 pKa = 4.19AMDD457 pKa = 3.25WTIASQTKK465 pKa = 9.49TIVNAADD472 pKa = 3.12GRR474 pKa = 11.84YY475 pKa = 7.51RR476 pKa = 11.84TAGTLMSGSRR486 pKa = 11.84LTTFLNTALNFIYY499 pKa = 9.88MDD501 pKa = 3.3IAGVFATPGFRR512 pKa = 11.84DD513 pKa = 3.74SVHH516 pKa = 6.61NGDD519 pKa = 5.1DD520 pKa = 3.32VLLTVEE526 pKa = 4.79SMSTACSIHH535 pKa = 7.26AKK537 pKa = 8.69MAAINARR544 pKa = 11.84AQAAKK549 pKa = 10.35CNAFSIGEE557 pKa = 4.01FLRR560 pKa = 11.84VEE562 pKa = 4.35HH563 pKa = 6.83KK564 pKa = 10.67LSKK567 pKa = 8.94QTGNGAQYY575 pKa = 9.04LTRR578 pKa = 11.84ACATTVHH585 pKa = 6.16SRR587 pKa = 11.84IEE589 pKa = 3.94SQAPVKK595 pKa = 9.12LTSMLGAYY603 pKa = 9.98RR604 pKa = 11.84NRR606 pKa = 11.84QADD609 pKa = 3.16IEE611 pKa = 4.15ARR613 pKa = 11.84AFIPDD618 pKa = 3.62HH619 pKa = 6.87VKK621 pKa = 10.75DD622 pKa = 3.82AMVKK626 pKa = 10.0HH627 pKa = 6.47VINKK631 pKa = 9.09ASTVFTVPKK640 pKa = 8.4EE641 pKa = 4.21TVEE644 pKa = 4.7AIYY647 pKa = 9.83HH648 pKa = 4.89AHH650 pKa = 5.24VVVGGVSTDD659 pKa = 2.89SDD661 pKa = 3.7ASVGVEE667 pKa = 4.19FEE669 pKa = 3.93EE670 pKa = 5.79HH671 pKa = 5.49PAQRR675 pKa = 11.84IFEE678 pKa = 4.6DD679 pKa = 3.62EE680 pKa = 4.26DD681 pKa = 3.78GKK683 pKa = 11.16EE684 pKa = 4.02KK685 pKa = 10.7QLLPGIIDD693 pKa = 3.68YY694 pKa = 11.42SDD696 pKa = 3.93LLASKK701 pKa = 10.67YY702 pKa = 10.97EE703 pKa = 3.96GMLSDD708 pKa = 3.8KK709 pKa = 10.91VVFSAVMRR717 pKa = 11.84ATRR720 pKa = 11.84NQLAVTRR727 pKa = 11.84EE728 pKa = 3.79VEE730 pKa = 3.73IRR732 pKa = 11.84QISTPARR739 pKa = 11.84GKK741 pKa = 10.58FGLARR746 pKa = 11.84SLYY749 pKa = 9.77KK750 pKa = 10.47LYY752 pKa = 11.06KK753 pKa = 10.11NVLQIPFVSQARR765 pKa = 11.84FLGVPPISLATPYY778 pKa = 9.59QAHH781 pKa = 4.84VLKK784 pKa = 10.63RR785 pKa = 11.84VVGNVQDD792 pKa = 3.92PLRR795 pKa = 11.84ALRR798 pKa = 11.84ILLL801 pKa = 4.01

Molecular weight:
90.38 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1482

681

801

741.0

83.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.232 ± 1.448

1.889 ± 0.301

5.466 ± 0.021

6.005 ± 0.106

3.509 ± 0.086

5.803 ± 0.623

2.767 ± 0.658

5.061 ± 0.045

5.196 ± 0.421

8.907 ± 1.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.239 ± 0.198

3.981 ± 0.566

4.251 ± 0.87

3.306 ± 0.143

6.478 ± 0.588

6.95 ± 0.257

5.938 ± 0.427

7.895 ± 0.17

1.417 ± 0.226

3.711 ± 0.122

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski