Red clover powdery mildew-associated totivirus 1
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3Q2B6|A0A0S3Q2B6_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 1 OX=1714362 GN=CP PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 6.35 KK3 pKa = 10.37 IIKK6 pKa = 9.92 HH7 pKa = 4.71 VFEE10 pKa = 4.59 SEE12 pKa = 3.85 GMAGMPIKK20 pKa = 10.68 HH21 pKa = 6.3 EE22 pKa = 4.42 SGNYY26 pKa = 6.65 NTLLKK31 pKa = 10.99 GNLAVDD37 pKa = 3.59 VNGDD41 pKa = 3.49 KK42 pKa = 10.84 NIKK45 pKa = 9.1 KK46 pKa = 8.67 LTMAQSMTYY55 pKa = 10.1 LGRR58 pKa = 11.84 STVVMGSGSSDD69 pKa = 3.34 LEE71 pKa = 4.6 GINKK75 pKa = 9.2 FCLTPEE81 pKa = 4.26 GNLNIVNVLATVAKK95 pKa = 9.97 EE96 pKa = 3.91 SGMPLNEE103 pKa = 3.76 QHH105 pKa = 7.15 HH106 pKa = 6.3 KK107 pKa = 10.43 LEE109 pKa = 4.17 YY110 pKa = 8.84 MKK112 pKa = 10.84 TMSWADD118 pKa = 3.24 NHH120 pKa = 6.71 VSLLVNLMRR129 pKa = 11.84 FAILLEE135 pKa = 4.23 LSEE138 pKa = 4.49 SSGGVGLKK146 pKa = 10.45 GALPKK151 pKa = 11.16 YY152 pKa = 9.51 NDD154 pKa = 3.16 GVVAVNRR161 pKa = 11.84 TALLPEE167 pKa = 4.97 EE168 pKa = 4.49 YY169 pKa = 9.72 PVGGDD174 pKa = 3.24 MTWFTRR180 pKa = 11.84 FSRR183 pKa = 11.84 QDD185 pKa = 3.08 MPDD188 pKa = 3.09 VVRR191 pKa = 11.84 VEE193 pKa = 4.89 EE194 pKa = 4.38 YY195 pKa = 10.34 CAPQSDD201 pKa = 4.1 VCLDD205 pKa = 3.6 LRR207 pKa = 11.84 SLTEE211 pKa = 3.91 TEE213 pKa = 3.89 AVIVLLCCGKK223 pKa = 9.65 WYY225 pKa = 9.87 RR226 pKa = 11.84 QSRR229 pKa = 11.84 FLLDD233 pKa = 3.85 FDD235 pKa = 5.52 CPILTSKK242 pKa = 10.74 LYY244 pKa = 10.36 YY245 pKa = 10.51 RR246 pKa = 11.84 FDD248 pKa = 3.28 KK249 pKa = 11.08 RR250 pKa = 11.84 IDD252 pKa = 3.26 IGNFDD257 pKa = 3.55 QTSDD261 pKa = 3.82 GRR263 pKa = 11.84 VTQPKK268 pKa = 9.83 LPKK271 pKa = 10.27 AKK273 pKa = 9.97 EE274 pKa = 3.83 VWRR277 pKa = 11.84 TMSRR281 pKa = 11.84 YY282 pKa = 8.96 LAANRR287 pKa = 11.84 LYY289 pKa = 10.67 GQYY292 pKa = 8.97 STALCLVAQTMCQFIPATAEE312 pKa = 3.9 GNVWLTQQRR321 pKa = 11.84 VIHH324 pKa = 6.4 LPALKK329 pKa = 9.82 SVRR332 pKa = 11.84 GALPFINEE340 pKa = 4.44 DD341 pKa = 3.42 EE342 pKa = 4.46 PALVSQRR349 pKa = 11.84 SLNEE353 pKa = 3.22 WGAFSTDD360 pKa = 2.95 VTKK363 pKa = 11.37 YY364 pKa = 10.68 NLLGNILVQAVQTGIAVRR382 pKa = 11.84 ALRR385 pKa = 11.84 FRR387 pKa = 11.84 NEE389 pKa = 4.35 DD390 pKa = 3.46 NPEE393 pKa = 3.93 DD394 pKa = 4.13 LYY396 pKa = 10.63 RR397 pKa = 11.84 TYY399 pKa = 10.96 DD400 pKa = 3.35 TVTNPAVMYY409 pKa = 9.99 GALCSEE415 pKa = 5.07 ALRR418 pKa = 11.84 CPVPLAGMSGVFVEE432 pKa = 6.18 FEE434 pKa = 4.16 LPDD437 pKa = 3.33 EE438 pKa = 4.42 RR439 pKa = 11.84 EE440 pKa = 4.04 EE441 pKa = 4.33 VEE443 pKa = 5.07 LEE445 pKa = 4.21 FPVLVYY451 pKa = 10.5 DD452 pKa = 4.61 PKK454 pKa = 10.85 RR455 pKa = 11.84 CGSTIEE461 pKa = 3.92 KK462 pKa = 10.51 RR463 pKa = 11.84 RR464 pKa = 11.84 DD465 pKa = 3.22 TEE467 pKa = 4.06 YY468 pKa = 11.12 LQLNSIPMAGVPTLLLPLNPIGKK491 pKa = 8.65 MSPFNLRR498 pKa = 11.84 GVFDD502 pKa = 3.65 TADD505 pKa = 3.6 LKK507 pKa = 9.1 RR508 pKa = 11.84 TKK510 pKa = 10.04 MGWEE514 pKa = 3.86 TDD516 pKa = 4.06 LYY518 pKa = 10.99 SAWSWAWLCRR528 pKa = 11.84 LCGYY532 pKa = 9.55 DD533 pKa = 2.77 IQMRR537 pKa = 11.84 QQYY540 pKa = 8.79 GQSCGSQGDD549 pKa = 4.1 YY550 pKa = 11.25 SPDD553 pKa = 3.93 ASWTWPILGSVGHH566 pKa = 6.34 NGIVCISKK574 pKa = 10.32 LVEE577 pKa = 4.35 RR578 pKa = 11.84 GNSFIDD584 pKa = 4.19 LPPLHH589 pKa = 7.11 SKK591 pKa = 9.84 SCHH594 pKa = 4.99 SVINYY599 pKa = 8.54 KK600 pKa = 10.46 FSINSHH606 pKa = 4.99 SVSLPEE612 pKa = 3.81 RR613 pKa = 11.84 RR614 pKa = 11.84 QPEE617 pKa = 4.74 LIGDD621 pKa = 4.47 RR622 pKa = 11.84 YY623 pKa = 10.56 SAAPVLNLRR632 pKa = 11.84 HH633 pKa = 6.15 INIATTTEE641 pKa = 3.92 IIKK644 pKa = 10.76 LKK646 pKa = 10.81 GFIEE650 pKa = 4.45 RR651 pKa = 11.84 SEE653 pKa = 4.26 SDD655 pKa = 3.48 FRR657 pKa = 11.84 FADD660 pKa = 3.98 DD661 pKa = 3.69 VQAGQIPPPEE671 pKa = 4.34 VPHH674 pKa = 5.95 VASDD678 pKa = 3.72 VIEE681 pKa = 4.09
Molecular weight: 76.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.475
IPC2_protein 5.55
IPC_protein 5.55
Toseland 5.753
ProMoST 5.741
Dawson 5.715
Bjellqvist 5.779
Wikipedia 5.677
Rodwell 5.677
Grimsley 5.817
Solomon 5.715
Lehninger 5.69
Nozaki 5.931
DTASelect 6.109
Thurlkill 6.02
EMBOSS 5.982
Sillero 6.02
Patrickios 3.973
IPC_peptide 5.728
IPC2_peptide 6.02
IPC2.peptide.svr19 6.027
Protein with the highest isoelectric point:
>tr|A0A0S3Q2B6|A0A0S3Q2B6_9VIRU Capsid protein OS=Red clover powdery mildew-associated totivirus 1 OX=1714362 GN=CP PE=4 SV=1
MM1 pKa = 7.35 WPVFTRR7 pKa = 11.84 MSEE10 pKa = 3.84 NRR12 pKa = 11.84 LEE14 pKa = 4.06 VVDD17 pKa = 3.76 RR18 pKa = 11.84 HH19 pKa = 5.93 KK20 pKa = 10.51 CTHH23 pKa = 5.51 VLVGVEE29 pKa = 4.44 EE30 pKa = 5.03 DD31 pKa = 3.53 TMTWQGNDD39 pKa = 2.33 IMYY42 pKa = 10.43 AGQEE46 pKa = 4.29 CVRR49 pKa = 11.84 TCSYY53 pKa = 11.55 GDD55 pKa = 3.31 GGTRR59 pKa = 11.84 AHH61 pKa = 6.44 YY62 pKa = 10.53 IKK64 pKa = 10.18 MNKK67 pKa = 8.66 PVSLLPPIVKK77 pKa = 9.36 QRR79 pKa = 11.84 LSAAYY84 pKa = 10.12 SMVGGYY90 pKa = 10.38 VYY92 pKa = 11.01 GDD94 pKa = 3.54 KK95 pKa = 11.27 DD96 pKa = 3.29 EE97 pKa = 4.67 LASVRR102 pKa = 11.84 QILKK106 pKa = 9.66 PLKK109 pKa = 9.55 QNYY112 pKa = 8.55 KK113 pKa = 9.77 HH114 pKa = 6.65 DD115 pKa = 3.94 VKK117 pKa = 11.0 FSIRR121 pKa = 11.84 SLTYY125 pKa = 9.96 KK126 pKa = 10.53 KK127 pKa = 10.5 SATTGEE133 pKa = 4.3 HH134 pKa = 5.44 HH135 pKa = 5.79 THH137 pKa = 4.78 YY138 pKa = 10.21 TPMEE142 pKa = 4.15 VYY144 pKa = 10.89 NIVTCDD150 pKa = 2.83 ARR152 pKa = 11.84 RR153 pKa = 11.84 RR154 pKa = 11.84 QLVLSMNARR163 pKa = 11.84 LRR165 pKa = 11.84 QMHH168 pKa = 7.4 GITEE172 pKa = 4.53 AFAATILMYY181 pKa = 10.39 IATARR186 pKa = 11.84 EE187 pKa = 4.18 SVAVMIATSSTLWARR202 pKa = 11.84 NVDD205 pKa = 3.91 LTLLRR210 pKa = 11.84 LKK212 pKa = 10.26 EE213 pKa = 3.92 CSVPFKK219 pKa = 10.6 SLHH222 pKa = 5.93 HH223 pKa = 7.14 ADD225 pKa = 5.26 LIDD228 pKa = 3.67 LTEE231 pKa = 3.99 MFEE234 pKa = 4.76 LLSLANRR241 pKa = 11.84 GYY243 pKa = 11.2 GAVDD247 pKa = 3.0 WRR249 pKa = 11.84 AEE251 pKa = 4.02 KK252 pKa = 10.02 EE253 pKa = 4.04 HH254 pKa = 6.09 RR255 pKa = 11.84 TKK257 pKa = 10.83 VDD259 pKa = 3.99 DD260 pKa = 4.36 IKK262 pKa = 11.5 VKK264 pKa = 10.83 SIDD267 pKa = 3.61 VYY269 pKa = 10.74 RR270 pKa = 11.84 SAMKK274 pKa = 10.13 IFEE277 pKa = 4.41 MGNKK281 pKa = 9.85 NGFRR285 pKa = 11.84 YY286 pKa = 9.71 KK287 pKa = 10.44 KK288 pKa = 10.62 LSIKK292 pKa = 10.7 DD293 pKa = 3.46 FTASRR298 pKa = 11.84 WEE300 pKa = 3.82 WAPAGSVHH308 pKa = 6.1 SQHH311 pKa = 6.62 EE312 pKa = 4.18 DD313 pKa = 2.94 DD314 pKa = 5.55 RR315 pKa = 11.84 VYY317 pKa = 10.69 IDD319 pKa = 3.46 PSNYY323 pKa = 7.85 RR324 pKa = 11.84 HH325 pKa = 5.19 RR326 pKa = 11.84 TKK328 pKa = 10.6 FVTLNSMPARR338 pKa = 11.84 HH339 pKa = 6.4 IEE341 pKa = 4.13 TFLEE345 pKa = 4.28 RR346 pKa = 11.84 EE347 pKa = 4.35 PEE349 pKa = 3.87 IQAWPSEE356 pKa = 4.03 KK357 pKa = 10.11 IEE359 pKa = 3.83 WGKK362 pKa = 10.31 RR363 pKa = 11.84 RR364 pKa = 11.84 AIYY367 pKa = 10.16 GVDD370 pKa = 3.45 LTSATLAHH378 pKa = 6.82 LAMYY382 pKa = 10.16 NCEE385 pKa = 3.78 DD386 pKa = 3.68 VFRR389 pKa = 11.84 HH390 pKa = 5.87 RR391 pKa = 11.84 FPVGKK396 pKa = 9.25 EE397 pKa = 3.73 AEE399 pKa = 3.9 ASRR402 pKa = 11.84 VHH404 pKa = 5.79 KK405 pKa = 10.15 RR406 pKa = 11.84 LRR408 pKa = 11.84 AMCEE412 pKa = 3.92 GEE414 pKa = 4.02 EE415 pKa = 4.27 TFCYY419 pKa = 10.82 DD420 pKa = 4.02 FDD422 pKa = 5.24 DD423 pKa = 5.71 FNAQHH428 pKa = 6.05 STDD431 pKa = 3.25 AMIAVLCAYY440 pKa = 9.48 RR441 pKa = 11.84 DD442 pKa = 3.51 KK443 pKa = 11.38 YY444 pKa = 9.67 AADD447 pKa = 3.31 MSAVQRR453 pKa = 11.84 EE454 pKa = 4.19 AMDD457 pKa = 3.25 WTIASQTKK465 pKa = 9.49 TIVNAADD472 pKa = 3.12 GRR474 pKa = 11.84 YY475 pKa = 7.51 RR476 pKa = 11.84 TAGTLMSGSRR486 pKa = 11.84 LTTFLNTALNFIYY499 pKa = 9.88 MDD501 pKa = 3.3 IAGVFATPGFRR512 pKa = 11.84 DD513 pKa = 3.74 SVHH516 pKa = 6.61 NGDD519 pKa = 5.1 DD520 pKa = 3.32 VLLTVEE526 pKa = 4.79 SMSTACSIHH535 pKa = 7.26 AKK537 pKa = 8.69 MAAINARR544 pKa = 11.84 AQAAKK549 pKa = 10.35 CNAFSIGEE557 pKa = 4.01 FLRR560 pKa = 11.84 VEE562 pKa = 4.35 HH563 pKa = 6.83 KK564 pKa = 10.67 LSKK567 pKa = 8.94 QTGNGAQYY575 pKa = 9.04 LTRR578 pKa = 11.84 ACATTVHH585 pKa = 6.16 SRR587 pKa = 11.84 IEE589 pKa = 3.94 SQAPVKK595 pKa = 9.12 LTSMLGAYY603 pKa = 9.98 RR604 pKa = 11.84 NRR606 pKa = 11.84 QADD609 pKa = 3.16 IEE611 pKa = 4.15 ARR613 pKa = 11.84 AFIPDD618 pKa = 3.62 HH619 pKa = 6.87 VKK621 pKa = 10.75 DD622 pKa = 3.82 AMVKK626 pKa = 10.0 HH627 pKa = 6.47 VINKK631 pKa = 9.09 ASTVFTVPKK640 pKa = 8.4 EE641 pKa = 4.21 TVEE644 pKa = 4.7 AIYY647 pKa = 9.83 HH648 pKa = 4.89 AHH650 pKa = 5.24 VVVGGVSTDD659 pKa = 2.89 SDD661 pKa = 3.7 ASVGVEE667 pKa = 4.19 FEE669 pKa = 3.93 EE670 pKa = 5.79 HH671 pKa = 5.49 PAQRR675 pKa = 11.84 IFEE678 pKa = 4.6 DD679 pKa = 3.62 EE680 pKa = 4.26 DD681 pKa = 3.78 GKK683 pKa = 11.16 EE684 pKa = 4.02 KK685 pKa = 10.7 QLLPGIIDD693 pKa = 3.68 YY694 pKa = 11.42 SDD696 pKa = 3.93 LLASKK701 pKa = 10.67 YY702 pKa = 10.97 EE703 pKa = 3.96 GMLSDD708 pKa = 3.8 KK709 pKa = 10.91 VVFSAVMRR717 pKa = 11.84 ATRR720 pKa = 11.84 NQLAVTRR727 pKa = 11.84 EE728 pKa = 3.79 VEE730 pKa = 3.73 IRR732 pKa = 11.84 QISTPARR739 pKa = 11.84 GKK741 pKa = 10.58 FGLARR746 pKa = 11.84 SLYY749 pKa = 9.77 KK750 pKa = 10.47 LYY752 pKa = 11.06 KK753 pKa = 10.11 NVLQIPFVSQARR765 pKa = 11.84 FLGVPPISLATPYY778 pKa = 9.59 QAHH781 pKa = 4.84 VLKK784 pKa = 10.63 RR785 pKa = 11.84 VVGNVQDD792 pKa = 3.92 PLRR795 pKa = 11.84 ALRR798 pKa = 11.84 ILLL801 pKa = 4.01
Molecular weight: 90.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.079
IPC2_protein 8.185
IPC_protein 8.024
Toseland 8.244
ProMoST 8.58
Dawson 8.858
Bjellqvist 8.946
Wikipedia 8.931
Rodwell 8.946
Grimsley 8.448
Solomon 8.931
Lehninger 8.902
Nozaki 9.048
DTASelect 8.785
Thurlkill 8.858
EMBOSS 9.019
Sillero 9.092
Patrickios 4.52
IPC_peptide 8.916
IPC2_peptide 7.746
IPC2.peptide.svr19 7.751
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1482
681
801
741.0
83.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.232 ± 1.448
1.889 ± 0.301
5.466 ± 0.021
6.005 ± 0.106
3.509 ± 0.086
5.803 ± 0.623
2.767 ± 0.658
5.061 ± 0.045
5.196 ± 0.421
8.907 ± 1.091
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.239 ± 0.198
3.981 ± 0.566
4.251 ± 0.87
3.306 ± 0.143
6.478 ± 0.588
6.95 ± 0.257
5.938 ± 0.427
7.895 ± 0.17
1.417 ± 0.226
3.711 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here