Mycobacterium phage Cain

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222ZLY5|A0A222ZLY5_9CAUD Uncharacterized protein OS=Mycobacterium phage Cain OX=2015818 GN=89 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.35APSTIEE8 pKa = 4.15VPCPACGEE16 pKa = 4.49PIVLSIGFEE25 pKa = 4.21VAVPEE30 pKa = 4.73PGADD34 pKa = 3.28SAPVYY39 pKa = 9.59VTTPDD44 pKa = 3.49LADD47 pKa = 3.8RR48 pKa = 11.84AQAHH52 pKa = 6.24GEE54 pKa = 4.2VCPVLAGGGCC64 pKa = 3.37

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222ZMU6|A0A222ZMU6_9CAUD 3'-phosphate/5'-hydroxy nucleic acid ligase OS=Mycobacterium phage Cain OX=2015818 GN=92 PE=3 SV=1
MM1 pKa = 7.43TSTARR6 pKa = 11.84HH7 pKa = 5.62MSPAQARR14 pKa = 11.84AITLNLLRR22 pKa = 11.84DD23 pKa = 3.6HH24 pKa = 7.1GLTGWSVRR32 pKa = 11.84YY33 pKa = 10.17DD34 pKa = 3.43NARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84AGMCSYY45 pKa = 10.58RR46 pKa = 11.84EE47 pKa = 4.0RR48 pKa = 11.84VISLSKK54 pKa = 10.43PLMAQRR60 pKa = 11.84SYY62 pKa = 11.79DD63 pKa = 4.03DD64 pKa = 2.73TWMTITHH71 pKa = 7.33EE72 pKa = 4.07IAHH75 pKa = 6.4ALVGPSHH82 pKa = 6.24GHH84 pKa = 5.99DD85 pKa = 3.56AVWSAKK91 pKa = 9.5HH92 pKa = 6.04RR93 pKa = 11.84SLGGDD98 pKa = 3.53GKK100 pKa = 11.04RR101 pKa = 11.84CFEE104 pKa = 4.85HH105 pKa = 8.54LDD107 pKa = 3.63EE108 pKa = 4.72SAPWMGTCAHH118 pKa = 7.05GKK120 pKa = 9.03QFARR124 pKa = 11.84YY125 pKa = 7.48RR126 pKa = 11.84APKK129 pKa = 9.0QLHH132 pKa = 5.48GWRR135 pKa = 11.84CRR137 pKa = 11.84CVRR140 pKa = 11.84GGSPITWQTRR150 pKa = 11.84EE151 pKa = 3.77QRR153 pKa = 11.84EE154 pKa = 3.85TMAAVVAQAQARR166 pKa = 11.84RR167 pKa = 11.84AQPRR171 pKa = 11.84RR172 pKa = 11.84VEE174 pKa = 4.29PLTSAPVGRR183 pKa = 11.84GVQLDD188 pKa = 3.84LFF190 pKa = 4.6

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

18802

28

1333

188.0

20.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.073 ± 0.479

1.122 ± 0.143

6.595 ± 0.27

5.691 ± 0.303

2.617 ± 0.141

8.925 ± 0.419

2.314 ± 0.177

3.808 ± 0.157

3.228 ± 0.164

8.393 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.149 ± 0.101

2.856 ± 0.152

5.781 ± 0.227

3.159 ± 0.161

7.111 ± 0.339

5.015 ± 0.188

5.733 ± 0.194

7.919 ± 0.223

2.138 ± 0.097

2.372 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski