Gordonia phage Ewald

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nyceiraevirus; unclassified Nyceiraevirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T4V9|A0A4D6T4V9_9CAUD Major tail protein OS=Gordonia phage Ewald OX=2572525 GN=17 PE=4 SV=1
MM1 pKa = 7.16SAEE4 pKa = 3.98PVVGEE9 pKa = 4.72FIDD12 pKa = 3.54NARR15 pKa = 11.84VLGRR19 pKa = 11.84HH20 pKa = 5.38DD21 pKa = 3.55RR22 pKa = 11.84CRR24 pKa = 11.84AAIDD28 pKa = 4.0RR29 pKa = 11.84FTQRR33 pKa = 11.84PRR35 pKa = 11.84YY36 pKa = 9.01CEE38 pKa = 3.96LEE40 pKa = 4.23RR41 pKa = 11.84GHH43 pKa = 7.26DD44 pKa = 4.02GLHH47 pKa = 6.77ADD49 pKa = 4.39CSLSGLVLMTWTDD62 pKa = 3.3EE63 pKa = 4.03EE64 pKa = 4.65AAVCTVDD71 pKa = 4.34IDD73 pKa = 4.07DD74 pKa = 4.8GYY76 pKa = 11.33EE77 pKa = 3.76YY78 pKa = 10.9GYY80 pKa = 11.02DD81 pKa = 3.17EE82 pKa = 4.46YY83 pKa = 11.53AAVGGGTT90 pKa = 3.42

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T501|A0A4D6T501_9CAUD Uncharacterized protein OS=Gordonia phage Ewald OX=2572525 GN=52 PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84TRR5 pKa = 11.84PPRR8 pKa = 11.84TCRR11 pKa = 11.84YY12 pKa = 9.41CRR14 pKa = 11.84LPVLWWRR21 pKa = 11.84NANRR25 pKa = 11.84DD26 pKa = 2.91GSICVDD32 pKa = 3.86VSPDD36 pKa = 4.34DD37 pKa = 3.74NGTVQKK43 pKa = 10.93VVTRR47 pKa = 11.84PAGEE51 pKa = 4.01QPVVWGRR58 pKa = 11.84RR59 pKa = 11.84LTGLALATAVEE70 pKa = 4.32AGEE73 pKa = 4.3QLFTLHH79 pKa = 6.56ATTCPARR86 pKa = 11.84KK87 pKa = 8.89EE88 pKa = 4.19RR89 pKa = 11.84NPKK92 pKa = 10.17PEE94 pKa = 4.22GLQIAWPTNNPRR106 pKa = 11.84RR107 pKa = 3.89

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

13335

49

1837

242.5

26.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.976 ± 0.669

0.78 ± 0.156

7.012 ± 0.263

5.654 ± 0.304

2.452 ± 0.152

9.006 ± 0.575

1.905 ± 0.195

4.312 ± 0.213

3.135 ± 0.274

7.747 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.126

2.887 ± 0.221

6.239 ± 0.427

3.69 ± 0.361

7.559 ± 0.443

5.474 ± 0.307

6.449 ± 0.264

7.214 ± 0.378

2.115 ± 0.236

2.107 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski