Viola virus
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A343X9V8|A0A343X9V8_9VIRU Replicase OS=Viola virus OX=2182586 PE=4 SV=1
MM1 pKa = 7.63 GDD3 pKa = 4.56 DD4 pKa = 4.17 YY5 pKa = 12.01 DD6 pKa = 4.8 GLTPDD11 pKa = 6.21 FIIEE15 pKa = 4.22 TNTGVFHH22 pKa = 6.32 VVEE25 pKa = 5.07 FTTTRR30 pKa = 11.84 AGEE33 pKa = 4.07 HH34 pKa = 6.07 SASEE38 pKa = 3.71 AAYY41 pKa = 8.96 TKK43 pKa = 9.69 IAKK46 pKa = 10.17 YY47 pKa = 10.22 EE48 pKa = 3.97 IACQARR54 pKa = 11.84 SQGRR58 pKa = 11.84 SVCLSVISVHH68 pKa = 7.5 RR69 pKa = 11.84 DD70 pKa = 3.53 GVWSNLEE77 pKa = 3.89 LTEE80 pKa = 4.15 QEE82 pKa = 3.97 VDD84 pKa = 3.62 EE85 pKa = 4.37 LCYY88 pKa = 9.93 RR89 pKa = 11.84 YY90 pKa = 10.58 RR91 pKa = 11.84 MALSIFEE98 pKa = 4.48 EE99 pKa = 4.31 IKK101 pKa = 10.89 KK102 pKa = 9.32 EE103 pKa = 3.87 FPKK106 pKa = 10.62 IQDD109 pKa = 3.16 SDD111 pKa = 3.52 EE112 pKa = 4.17 DD113 pKa = 3.52 LARR116 pKa = 11.84 IVRR119 pKa = 11.84 QINGVLSMISMDD131 pKa = 3.4 WDD133 pKa = 3.39 RR134 pKa = 11.84 TEE136 pKa = 4.52 SKK138 pKa = 10.78 FPLFRR143 pKa = 11.84 RR144 pKa = 11.84 DD145 pKa = 3.45 VIEE148 pKa = 4.15 TFPSFVPDD156 pKa = 3.19 EE157 pKa = 4.82 AYY159 pKa = 10.57 LSKK162 pKa = 9.46 ITSMALKK169 pKa = 10.08 KK170 pKa = 10.54 AQEE173 pKa = 4.33 DD174 pKa = 3.99 LVEE177 pKa = 4.24 SAFLKK182 pKa = 9.55 TAEE185 pKa = 4.21 SDD187 pKa = 3.71 EE188 pKa = 4.28 EE189 pKa = 4.03 RR190 pKa = 11.84 LKK192 pKa = 11.54 LNLQEE197 pKa = 5.23 CRR199 pKa = 11.84 NKK201 pKa = 10.77 CQDD204 pKa = 3.57 FKK206 pKa = 11.67 DD207 pKa = 3.78 QFGKK211 pKa = 10.46 GHH213 pKa = 5.89 SFRR216 pKa = 11.84 DD217 pKa = 3.36 RR218 pKa = 11.84 DD219 pKa = 3.92 HH220 pKa = 6.77 QKK222 pKa = 9.06 STVQIPPWVTYY233 pKa = 10.01 IGHH236 pKa = 6.47 EE237 pKa = 4.31 GKK239 pKa = 10.12 DD240 pKa = 3.48 LRR242 pKa = 11.84 NLRR245 pKa = 11.84 DD246 pKa = 3.46 LSVEE250 pKa = 3.97 GDD252 pKa = 3.44 HH253 pKa = 7.28 PMVKK257 pKa = 8.91 VWRR260 pKa = 11.84 TVVEE264 pKa = 3.9 KK265 pKa = 10.88 AVFEE269 pKa = 4.79 EE270 pKa = 4.29 ISRR273 pKa = 11.84 MYY275 pKa = 10.78 DD276 pKa = 3.24 DD277 pKa = 4.99 PKK279 pKa = 11.48 EE280 pKa = 3.98 EE281 pKa = 3.84 LQYY284 pKa = 11.82 ALSGEE289 pKa = 4.47 LKK291 pKa = 10.46 RR292 pKa = 11.84 DD293 pKa = 3.76 DD294 pKa = 4.18 QKK296 pKa = 11.87 SKK298 pKa = 7.42 YY299 pKa = 9.71 HH300 pKa = 5.83 RR301 pKa = 11.84 VKK303 pKa = 9.91 VTLDD307 pKa = 3.59 AEE309 pKa = 4.28 SSDD312 pKa = 3.94 YY313 pKa = 11.49 VNSLGVEE320 pKa = 4.5 GKK322 pKa = 9.86 SNRR325 pKa = 11.84 HH326 pKa = 3.97 KK327 pKa = 9.78 TAVTEE332 pKa = 3.8 ARR334 pKa = 11.84 ARR336 pKa = 11.84 GKK338 pKa = 10.68 LGFSLDD344 pKa = 3.79 HH345 pKa = 6.73 DD346 pKa = 4.07 TSSIEE351 pKa = 4.55 DD352 pKa = 3.86 FLNEE356 pKa = 4.28 SNNNHH361 pKa = 6.39 LLEE364 pKa = 4.57 PVEE367 pKa = 4.15 EE368 pKa = 4.73 LYY370 pKa = 11.23 SPLSADD376 pKa = 3.35 EE377 pKa = 4.36 EE378 pKa = 4.32 LRR380 pKa = 11.84 KK381 pKa = 9.76 LAGSIHH387 pKa = 5.37 QPRR390 pKa = 11.84 LFTLKK395 pKa = 10.86 GEE397 pKa = 4.28 NEE399 pKa = 4.01 FLRR402 pKa = 11.84 AHH404 pKa = 5.95 KK405 pKa = 10.53 QFMASRR411 pKa = 11.84 LGSWTQMVSVIGAEE425 pKa = 4.24 LSASVKK431 pKa = 8.09 QHH433 pKa = 5.44 VGPGQFVVKK442 pKa = 10.34 RR443 pKa = 11.84 LMDD446 pKa = 3.56 SAIYY450 pKa = 10.56 LLIKK454 pKa = 8.48 PTSSKK459 pKa = 10.56 GHH461 pKa = 6.0 IFVSFAVEE469 pKa = 3.88 KK470 pKa = 10.67 QYY472 pKa = 11.57 LEE474 pKa = 3.99 STLINSKK481 pKa = 10.11 LFRR484 pKa = 11.84 DD485 pKa = 3.99 YY486 pKa = 11.28 FDD488 pKa = 5.35 AGDD491 pKa = 4.01 LFVTDD496 pKa = 4.27 FTSFKK501 pKa = 10.56 LSKK504 pKa = 9.57 LTNLCKK510 pKa = 10.09 TNSLIEE516 pKa = 4.16 CALFFWTEE524 pKa = 3.88 AYY526 pKa = 9.86 GFPCWEE532 pKa = 4.23 AMKK535 pKa = 10.26 ILPKK539 pKa = 10.55 DD540 pKa = 3.49 RR541 pKa = 11.84 STSATDD547 pKa = 3.36 AVQMAKK553 pKa = 10.11 ISLLTLLEE561 pKa = 4.56 DD562 pKa = 3.6 KK563 pKa = 11.11 ARR565 pKa = 11.84 TEE567 pKa = 4.21 EE568 pKa = 4.29 LQTMMRR574 pKa = 11.84 YY575 pKa = 9.9 IMMEE579 pKa = 3.99 GFVSQPEE586 pKa = 3.93 IPKK589 pKa = 7.44 PHH591 pKa = 7.08 KK592 pKa = 9.86 MISKK596 pKa = 9.61 LPSVLRR602 pKa = 11.84 SEE604 pKa = 4.51 LQVFLCNRR612 pKa = 11.84 VFLSMEE618 pKa = 4.84 RR619 pKa = 11.84 IASKK623 pKa = 10.1 PFRR626 pKa = 11.84 ISKK629 pKa = 10.41 SEE631 pKa = 3.87 GQITWSGLFNPLTGSVVRR649 pKa = 11.84 DD650 pKa = 3.65 LQPVISACYY659 pKa = 9.92 NGYY662 pKa = 10.04 FKK664 pKa = 11.13 NKK666 pKa = 10.17 EE667 pKa = 3.86 EE668 pKa = 4.02 DD669 pKa = 3.73 TEE671 pKa = 4.42 PSVLSAMYY679 pKa = 10.13 KK680 pKa = 10.19 KK681 pKa = 10.26 IIEE684 pKa = 4.68 LEE686 pKa = 3.95 HH687 pKa = 6.64 LMPMDD692 pKa = 4.13 EE693 pKa = 4.45 VNLGLGDD700 pKa = 3.76 PVNPEE705 pKa = 3.33 MHH707 pKa = 6.41 EE708 pKa = 4.11 FSRR711 pKa = 11.84 SYY713 pKa = 10.83 LKK715 pKa = 10.66 RR716 pKa = 11.84 ITDD719 pKa = 3.23 HH720 pKa = 6.8 GKK722 pKa = 9.02 QLLSRR727 pKa = 11.84 IYY729 pKa = 10.51 GPNFMSLIEE738 pKa = 3.98 DD739 pKa = 3.61 QIMKK743 pKa = 10.0 EE744 pKa = 3.51 ISLITLEE751 pKa = 4.77 RR752 pKa = 11.84 LATLKK757 pKa = 9.96 ATSNFDD763 pKa = 3.57 EE764 pKa = 4.0 TWYY767 pKa = 9.89 IYY769 pKa = 11.1 KK770 pKa = 10.2 DD771 pKa = 3.13 IKK773 pKa = 11.02 PNTTYY778 pKa = 11.01 SRR780 pKa = 11.84 DD781 pKa = 3.41 KK782 pKa = 11.11 LIVKK786 pKa = 7.35 MANYY790 pKa = 10.29 ADD792 pKa = 4.02 EE793 pKa = 4.57 GHH795 pKa = 6.24 VKK797 pKa = 10.48 AIEE800 pKa = 4.07 KK801 pKa = 10.51 FEE803 pKa = 4.28 SCMRR807 pKa = 11.84 IIEE810 pKa = 4.23 EE811 pKa = 3.97 RR812 pKa = 11.84 GAMHH816 pKa = 6.68 ICLFKK821 pKa = 10.56 KK822 pKa = 9.12 QQHH825 pKa = 5.59 GGLRR829 pKa = 11.84 EE830 pKa = 3.96 IYY832 pKa = 10.51 VLGAEE837 pKa = 4.19 EE838 pKa = 5.87 RR839 pKa = 11.84 IVQCIVEE846 pKa = 4.86 AISRR850 pKa = 11.84 SIGKK854 pKa = 7.92 FFPSDD859 pKa = 3.54 TLCNPGNKK867 pKa = 9.74 AKK869 pKa = 10.4 IPEE872 pKa = 4.16 THH874 pKa = 6.56 AVRR877 pKa = 11.84 ARR879 pKa = 11.84 KK880 pKa = 7.75 QAKK883 pKa = 8.08 GAAWTTSTSDD893 pKa = 5.25 DD894 pKa = 3.32 ARR896 pKa = 11.84 KK897 pKa = 8.78 WNQGHH902 pKa = 6.14 FVTKK906 pKa = 9.78 FAMMLMEE913 pKa = 4.89 FTNQKK918 pKa = 6.83 WWPVIIRR925 pKa = 11.84 GCSMFTNKK933 pKa = 9.17 RR934 pKa = 11.84 MMLNLRR940 pKa = 11.84 YY941 pKa = 9.76 LDD943 pKa = 3.07 ILYY946 pKa = 10.45 ARR948 pKa = 11.84 RR949 pKa = 11.84 EE950 pKa = 4.23 LEE952 pKa = 4.05 VDD954 pKa = 3.6 DD955 pKa = 5.06 EE956 pKa = 4.6 FAKK959 pKa = 10.76 AMHH962 pKa = 6.44 GAFHH966 pKa = 7.06 GEE968 pKa = 3.7 ISVPWAEE975 pKa = 4.73 KK976 pKa = 9.92 GMTYY980 pKa = 10.42 ISTKK984 pKa = 8.78 TGMMQGILHH993 pKa = 6.05 FTSSLLHH1000 pKa = 5.92 TLHH1003 pKa = 6.91 QEE1005 pKa = 4.89 FIRR1008 pKa = 11.84 SLSFKK1013 pKa = 10.62 VFNLKK1018 pKa = 9.48 VAPEE1022 pKa = 4.16 TSHH1025 pKa = 6.55 EE1026 pKa = 4.06 LVIDD1030 pKa = 3.9 MMQGSDD1036 pKa = 4.19 DD1037 pKa = 3.78 SSMIISFPCKK1047 pKa = 10.0 NEE1049 pKa = 3.88 SFIAKK1054 pKa = 9.47 CKK1056 pKa = 8.93 VASAISFRR1064 pKa = 11.84 IKK1066 pKa = 10.54 RR1067 pKa = 11.84 LLGLYY1072 pKa = 10.21 AGIYY1076 pKa = 9.33 PSEE1079 pKa = 4.51 KK1080 pKa = 9.31 STSNTDD1086 pKa = 3.34 FALEE1090 pKa = 4.19 YY1091 pKa = 10.32 NSEE1094 pKa = 4.57 FYY1096 pKa = 10.73 FHH1098 pKa = 6.51 SQHH1101 pKa = 5.68 VRR1103 pKa = 11.84 PTIRR1107 pKa = 11.84 WIAASCSLPEE1117 pKa = 4.12 VEE1119 pKa = 4.61 TLVARR1124 pKa = 11.84 QEE1126 pKa = 4.15 EE1127 pKa = 4.55 ASNLLTSVAEE1137 pKa = 4.4 GGGSFSLVAQIQQAQCTIHH1156 pKa = 6.35 YY1157 pKa = 9.18 MLMGMGVSSLFEE1169 pKa = 4.0 EE1170 pKa = 5.15 FIKK1173 pKa = 10.8 AIRR1176 pKa = 11.84 QWLDD1180 pKa = 2.93 PGLGFFLLDD1189 pKa = 3.32 NPYY1192 pKa = 10.86 AAGLGGFRR1200 pKa = 11.84 YY1201 pKa = 10.19 NLYY1204 pKa = 10.16 QAINRR1209 pKa = 11.84 TNLKK1213 pKa = 9.78 RR1214 pKa = 11.84 LYY1216 pKa = 10.83 AFFLRR1221 pKa = 11.84 KK1222 pKa = 9.13 VRR1224 pKa = 11.84 GMRR1227 pKa = 11.84 EE1228 pKa = 3.47 VDD1230 pKa = 3.54 DD1231 pKa = 4.98 EE1232 pKa = 4.75 GNEE1235 pKa = 4.12 SLIEE1239 pKa = 3.98 PEE1241 pKa = 4.32 ACSVSPGGALILSSSLRR1258 pKa = 11.84 WGSRR1262 pKa = 11.84 EE1263 pKa = 3.77 KK1264 pKa = 10.59 FRR1266 pKa = 11.84 KK1267 pKa = 9.98 LKK1269 pKa = 10.61 DD1270 pKa = 3.35 RR1271 pKa = 11.84 LHH1273 pKa = 8.08 IPEE1276 pKa = 4.68 DD1277 pKa = 2.97 WKK1279 pKa = 11.09 EE1280 pKa = 4.12 QINQRR1285 pKa = 11.84 PRR1287 pKa = 11.84 LLYY1290 pKa = 8.71 EE1291 pKa = 4.56 APLSGKK1297 pKa = 10.32 DD1298 pKa = 2.83 ITLRR1302 pKa = 11.84 IAEE1305 pKa = 4.59 KK1306 pKa = 9.64 VHH1308 pKa = 6.27 SPGIVSSLSTGNAVAKK1324 pKa = 10.51 VMASAVYY1331 pKa = 9.48 FLSASIFEE1339 pKa = 4.31 DD1340 pKa = 3.06 AGRR1343 pKa = 11.84 QEE1345 pKa = 4.07 YY1346 pKa = 9.78 HH1347 pKa = 6.88 AFTDD1351 pKa = 3.54 TKK1353 pKa = 11.17 YY1354 pKa = 11.38 SLLQKK1359 pKa = 9.22 MALYY1363 pKa = 10.55 EE1364 pKa = 4.17 GFKK1367 pKa = 10.68 GQAGVSKK1374 pKa = 11.14 DD1375 pKa = 3.34 DD1376 pKa = 3.72 MLFLFPNISEE1386 pKa = 4.72 LEE1388 pKa = 3.93 QLDD1391 pKa = 3.38 AVVYY1395 pKa = 9.76 NKK1397 pKa = 10.76 EE1398 pKa = 4.11 EE1399 pKa = 3.34 IHH1401 pKa = 5.88 YY1402 pKa = 8.85 LHH1404 pKa = 7.64 RR1405 pKa = 11.84 INQRR1409 pKa = 11.84 EE1410 pKa = 4.08 ATQTKK1415 pKa = 8.19 ITVFDD1420 pKa = 4.03 NVSQHH1425 pKa = 6.0 QIVAEE1430 pKa = 4.07 KK1431 pKa = 10.49 LVSDD1435 pKa = 3.99 KK1436 pKa = 11.07 WFGTNKK1442 pKa = 9.65 SHH1444 pKa = 7.88 LGRR1447 pKa = 11.84 VAFEE1451 pKa = 4.16 RR1452 pKa = 11.84 EE1453 pKa = 3.49 WRR1455 pKa = 11.84 RR1456 pKa = 11.84 LKK1458 pKa = 8.4 TTVSWLRR1465 pKa = 11.84 DD1466 pKa = 3.77 TPSEE1470 pKa = 4.03 TLEE1473 pKa = 4.04 ASPLDD1478 pKa = 3.34 SHH1480 pKa = 6.97 IQIKK1484 pKa = 10.66 NFFARR1489 pKa = 11.84 MEE1491 pKa = 4.46 GKK1493 pKa = 10.35 ARR1495 pKa = 11.84 TVRR1498 pKa = 11.84 ITGAPVKK1505 pKa = 10.28 KK1506 pKa = 10.13 RR1507 pKa = 11.84 SGVSKK1512 pKa = 10.1 ISLVIRR1518 pKa = 11.84 DD1519 pKa = 3.89 NFVKK1523 pKa = 10.46 CGHH1526 pKa = 7.1 LDD1528 pKa = 4.15 DD1529 pKa = 6.27 IEE1531 pKa = 4.39 DD1532 pKa = 3.79 LEE1534 pKa = 4.82 GIEE1537 pKa = 4.75 RR1538 pKa = 11.84 STSSEE1543 pKa = 3.97 VSKK1546 pKa = 11.08 HH1547 pKa = 4.9 FLFSILQGPYY1557 pKa = 8.1 TPEE1560 pKa = 3.97 NKK1562 pKa = 8.27 EE1563 pKa = 3.72 HH1564 pKa = 6.13 RR1565 pKa = 11.84 VIRR1568 pKa = 11.84 ALQEE1572 pKa = 3.9 LPEE1575 pKa = 4.16 VGIKK1579 pKa = 9.95 EE1580 pKa = 4.0 QDD1582 pKa = 2.96 RR1583 pKa = 11.84 KK1584 pKa = 8.98 TKK1586 pKa = 10.1 TNVIGILQNWSKK1598 pKa = 11.0 SKK1600 pKa = 10.88 SGVAQLIEE1608 pKa = 4.21 EE1609 pKa = 4.33 VGAGIIGGFIKK1620 pKa = 10.32 RR1621 pKa = 11.84 QRR1623 pKa = 11.84 SEE1625 pKa = 3.94 EE1626 pKa = 4.06 VEE1628 pKa = 4.03 GKK1630 pKa = 9.61 IVYY1633 pKa = 9.27 FGPGIWRR1640 pKa = 11.84 GTMDD1644 pKa = 3.67 GYY1646 pKa = 10.89 EE1647 pKa = 4.19 VQLEE1651 pKa = 4.42 VTNQKK1656 pKa = 10.12 HH1657 pKa = 4.41 EE1658 pKa = 4.08 RR1659 pKa = 11.84 TYY1661 pKa = 10.91 LKK1663 pKa = 10.61 SVTVSEE1669 pKa = 4.71 KK1670 pKa = 10.26 ASIWEE1675 pKa = 4.15 LCPSIRR1681 pKa = 11.84 SWCDD1685 pKa = 2.95 DD1686 pKa = 3.17 VGVINTRR1693 pKa = 11.84 DD1694 pKa = 3.54 VSKK1697 pKa = 10.41 HH1698 pKa = 4.8 ASRR1701 pKa = 11.84 SGLRR1705 pKa = 11.84 YY1706 pKa = 8.82 WMYY1709 pKa = 11.11 DD1710 pKa = 2.88 FRR1712 pKa = 11.84 AFGIEE1717 pKa = 4.21 KK1718 pKa = 10.31 PFGCPVYY1725 pKa = 9.56 ITQRR1729 pKa = 11.84 RR1730 pKa = 11.84 MNEE1733 pKa = 3.69 QFIMDD1738 pKa = 4.25 EE1739 pKa = 4.48 DD1740 pKa = 4.73 DD1741 pKa = 3.61 IKK1743 pKa = 11.47 LKK1745 pKa = 10.92 VRR1747 pKa = 11.84 GKK1749 pKa = 8.94 TINLYY1754 pKa = 10.4 VEE1756 pKa = 4.33 DD1757 pKa = 4.49 RR1758 pKa = 11.84 RR1759 pKa = 11.84 NHH1761 pKa = 4.3 IHH1763 pKa = 6.39 ILSYY1767 pKa = 10.0 TAGDD1771 pKa = 3.68 NDD1773 pKa = 3.66 ISPLITRR1780 pKa = 11.84 NNSDD1784 pKa = 3.52 PRR1786 pKa = 11.84 VQSLLSSYY1794 pKa = 9.49 MKK1796 pKa = 10.14 EE1797 pKa = 4.11 PSRR1800 pKa = 11.84 SWVTCQSLPFAFIDD1814 pKa = 4.98 LILKK1818 pKa = 9.91 LVEE1821 pKa = 4.26 GDD1823 pKa = 3.0 IHH1825 pKa = 8.22 KK1826 pKa = 10.6 PEE1828 pKa = 5.2 IDD1830 pKa = 3.76 SEE1832 pKa = 4.68 SLGKK1836 pKa = 9.96 IIKK1839 pKa = 8.5 TCLQSSLRR1847 pKa = 11.84 NKK1849 pKa = 8.51 VGSMFRR1855 pKa = 11.84 IMPSASTAYY1864 pKa = 10.34 AAIDD1868 pKa = 3.48 VDD1870 pKa = 4.48 DD1871 pKa = 4.93 VFDD1874 pKa = 5.23 IMLEE1878 pKa = 4.0 DD1879 pKa = 3.6 MTPDD1883 pKa = 3.29 MFKK1886 pKa = 9.97 STVQDD1891 pKa = 3.4 MEE1893 pKa = 5.01 LDD1895 pKa = 3.44 IQEE1898 pKa = 5.28 DD1899 pKa = 4.22 YY1900 pKa = 11.15 QSEE1903 pKa = 4.44 KK1904 pKa = 10.87 FDD1906 pKa = 3.64 FTDD1909 pKa = 3.26 IDD1911 pKa = 4.13 LFGPANFKK1919 pKa = 10.78 EE1920 pKa = 4.2 ITDD1923 pKa = 4.06 LAMISHH1929 pKa = 8.01 PLMDD1933 pKa = 5.28 DD1934 pKa = 4.21 FVSTLIAKK1942 pKa = 9.91 SSRR1945 pKa = 11.84 KK1946 pKa = 9.06 EE1947 pKa = 3.27 IRR1949 pKa = 11.84 AVVEE1953 pKa = 3.76 KK1954 pKa = 10.27 CVCSKK1959 pKa = 10.82 RR1960 pKa = 11.84 NEE1962 pKa = 3.91 QDD1964 pKa = 3.02 FKK1966 pKa = 11.51 LLYY1969 pKa = 9.77 RR1970 pKa = 11.84 ALGMNPKK1977 pKa = 10.05 DD1978 pKa = 3.25 IRR1980 pKa = 11.84 VEE1982 pKa = 3.98 EE1983 pKa = 4.8 SYY1985 pKa = 11.0 HH1986 pKa = 7.47 ADD1988 pKa = 3.45 AEE1990 pKa = 4.22 EE1991 pKa = 4.46 DD1992 pKa = 3.6 ADD1994 pKa = 4.95 LLGG1997 pKa = 4.55
Molecular weight: 227.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.977
IPC2_protein 5.919
IPC_protein 5.982
Toseland 6.351
ProMoST 6.351
Dawson 6.262
Bjellqvist 6.275
Wikipedia 6.275
Rodwell 6.249
Grimsley 6.504
Solomon 6.262
Lehninger 6.249
Nozaki 6.532
DTASelect 6.722
Thurlkill 6.737
EMBOSS 6.693
Sillero 6.634
Patrickios 4.393
IPC_peptide 6.275
IPC2_peptide 6.561
IPC2.peptide.svr19 6.599
Protein with the highest isoelectric point:
>tr|A0A343X9V8|A0A343X9V8_9VIRU Replicase OS=Viola virus OX=2182586 PE=4 SV=1
MM1 pKa = 7.21 FKK3 pKa = 10.71 KK4 pKa = 10.61 LVVLTLALGQGDD16 pKa = 4.89 CLYY19 pKa = 10.8 KK20 pKa = 9.77 ITSLNQGARR29 pKa = 11.84 HH30 pKa = 5.69 EE31 pKa = 4.67 LCMSNPTSFEE41 pKa = 4.01 SVIDD45 pKa = 3.45 YY46 pKa = 8.55 WGSMDD51 pKa = 3.79 RR52 pKa = 11.84 ALGEE56 pKa = 4.07 GDD58 pKa = 3.9 RR59 pKa = 11.84 EE60 pKa = 4.0 CDD62 pKa = 2.56 IGGGVKK68 pKa = 10.23 SYY70 pKa = 7.78 TTASTTKK77 pKa = 9.42 KK78 pKa = 10.22 LVRR81 pKa = 11.84 TVVDD85 pKa = 4.02 SMTALNMSCSIKK97 pKa = 10.63 DD98 pKa = 3.69 SPDD101 pKa = 3.28 KK102 pKa = 10.76 LIHH105 pKa = 6.63 LMFDD109 pKa = 3.47 AEE111 pKa = 4.56 YY112 pKa = 10.1 DD113 pKa = 3.51 GHH115 pKa = 6.8 LAPMIVSCIDD125 pKa = 3.57 GSAQGPLRR133 pKa = 11.84 IAGSHH138 pKa = 4.7 STNKK142 pKa = 9.84 IIHH145 pKa = 5.88 FPKK148 pKa = 10.02 GASVEE153 pKa = 4.43 EE154 pKa = 4.86 GTSPPEE160 pKa = 3.97 VSEE163 pKa = 4.85 KK164 pKa = 10.59 PDD166 pKa = 3.97 DD167 pKa = 4.29 LSDD170 pKa = 4.15 EE171 pKa = 3.46 ISYY174 pKa = 10.24 RR175 pKa = 11.84 IKK177 pKa = 10.97 SLTSGNNDD185 pKa = 3.49 PGSLVHH191 pKa = 6.75 VISSKK196 pKa = 10.27 DD197 pKa = 3.49 HH198 pKa = 6.01 EE199 pKa = 4.71 LSTLKK204 pKa = 10.98 EE205 pKa = 4.39 EE206 pKa = 4.29 INSLKK211 pKa = 10.67 SRR213 pKa = 11.84 LEE215 pKa = 3.82 HH216 pKa = 6.04 EE217 pKa = 4.38 RR218 pKa = 11.84 EE219 pKa = 3.79 DD220 pKa = 3.94 RR221 pKa = 11.84 DD222 pKa = 3.8 RR223 pKa = 11.84 LRR225 pKa = 11.84 AEE227 pKa = 4.13 HH228 pKa = 6.83 KK229 pKa = 10.62 AQIGHH234 pKa = 6.71 GDD236 pKa = 3.64 AEE238 pKa = 4.47 RR239 pKa = 11.84 KK240 pKa = 9.88 LLEE243 pKa = 4.31 DD244 pKa = 3.38 RR245 pKa = 11.84 LRR247 pKa = 11.84 ARR249 pKa = 11.84 EE250 pKa = 3.76 QDD252 pKa = 2.93 IHH254 pKa = 7.47 NDD256 pKa = 3.38 RR257 pKa = 11.84 AEE259 pKa = 4.01 LQQLKK264 pKa = 10.79 AKK266 pKa = 10.08 LRR268 pKa = 11.84 SLEE271 pKa = 3.98 NARR274 pKa = 11.84 VPVTTIAPKK283 pKa = 10.57 AITTALTVVSLLSAVGADD301 pKa = 3.64 LNNWTHH307 pKa = 6.24 AANRR311 pKa = 11.84 PGIGTFLFDD320 pKa = 5.8 GGDD323 pKa = 3.57 NDD325 pKa = 4.65 CASVTYY331 pKa = 8.29 GTKK334 pKa = 10.34 CSSFLTLLDD343 pKa = 4.1 DD344 pKa = 4.46 NKK346 pKa = 10.93 YY347 pKa = 10.09 PLFNTFVHH355 pKa = 6.85 KK356 pKa = 10.73 YY357 pKa = 9.48 SSLEE361 pKa = 3.75 AVTNNIIQKK370 pKa = 10.3 DD371 pKa = 3.85 DD372 pKa = 3.65 NGVCKK377 pKa = 10.08 TDD379 pKa = 3.53 AEE381 pKa = 4.35 RR382 pKa = 11.84 SNKK385 pKa = 9.69 KK386 pKa = 10.48 CFDD389 pKa = 3.23 EE390 pKa = 4.23 MKK392 pKa = 9.91 FMKK395 pKa = 10.03 MSCPNHH401 pKa = 5.7 MRR403 pKa = 11.84 SVHH406 pKa = 6.04 YY407 pKa = 9.85 IDD409 pKa = 5.25 SVGKK413 pKa = 9.66 LRR415 pKa = 11.84 GMICADD421 pKa = 3.24 EE422 pKa = 4.91 HH423 pKa = 6.84 EE424 pKa = 4.54 VTEE427 pKa = 5.7 DD428 pKa = 3.32 CLQCRR433 pKa = 11.84 KK434 pKa = 8.48 LTQKK438 pKa = 10.88 DD439 pKa = 3.96 AITKK443 pKa = 10.31 GSVSLQDD450 pKa = 3.38 VVCQKK455 pKa = 10.92 GSSNYY460 pKa = 10.22 EE461 pKa = 3.61 GTQPKK466 pKa = 9.94 IKK468 pKa = 9.56 GVCRR472 pKa = 11.84 IGEE475 pKa = 4.0 QDD477 pKa = 3.19 IKK479 pKa = 9.82 TCKK482 pKa = 9.75 RR483 pKa = 11.84 WTTNYY488 pKa = 7.02 EE489 pKa = 4.15 TVPFAVFSHH498 pKa = 5.95 SSGMKK503 pKa = 10.03 KK504 pKa = 10.39 LYY506 pKa = 10.4 LPHH509 pKa = 6.95 LKK511 pKa = 10.25 VKK513 pKa = 10.33 SVEE516 pKa = 4.32 VIDD519 pKa = 4.24 NDD521 pKa = 4.39 SFVCYY526 pKa = 8.29 QHH528 pKa = 6.69 VGQSSGNKK536 pKa = 9.17 KK537 pKa = 10.28 DD538 pKa = 3.6 ATDD541 pKa = 3.27 TRR543 pKa = 11.84 LMLRR547 pKa = 11.84 VAMTDD552 pKa = 3.51 CKK554 pKa = 11.06 NVDD557 pKa = 3.18 STKK560 pKa = 10.06 TKK562 pKa = 10.22 KK563 pKa = 9.42 CTGDD567 pKa = 3.4 EE568 pKa = 4.51 VFCSHH573 pKa = 7.32 FKK575 pKa = 9.71 CTQEE579 pKa = 4.7 YY580 pKa = 9.63 PDD582 pKa = 4.79 TYY584 pKa = 10.74 CLSAPGGGPLYY595 pKa = 11.01 VDD597 pKa = 4.73 IKK599 pKa = 10.92 GSWIMPACVGYY610 pKa = 7.96 EE611 pKa = 3.79 TLLVYY616 pKa = 10.58 RR617 pKa = 11.84 EE618 pKa = 4.03 VLPLTVKK625 pKa = 9.43 STQEE629 pKa = 4.03 CQSCYY634 pKa = 10.01 YY635 pKa = 10.16 EE636 pKa = 4.43 CRR638 pKa = 11.84 QDD640 pKa = 3.85 GIMIRR645 pKa = 11.84 STGFNILSATACSHH659 pKa = 6.19 GSCRR663 pKa = 11.84 SASQKK668 pKa = 10.68 SGTEE672 pKa = 3.21 ITIPYY677 pKa = 8.59 PGLSKK682 pKa = 10.88 SQGGEE687 pKa = 3.67 IGVHH691 pKa = 6.17 LAHH694 pKa = 7.82 DD695 pKa = 4.28 DD696 pKa = 3.71 TDD698 pKa = 3.7 VSSKK702 pKa = 9.85 LTISCKK708 pKa = 10.1 PRR710 pKa = 11.84 DD711 pKa = 3.43 QCEE714 pKa = 3.6 AHH716 pKa = 6.57 SCIFCWEE723 pKa = 3.8 VFINYY728 pKa = 8.57 QCHH731 pKa = 5.94 SFLSSFVICCLVIAVTGLALKK752 pKa = 10.7 ALFYY756 pKa = 10.66 TMKK759 pKa = 10.24 KK760 pKa = 8.36 VKK762 pKa = 9.67 SVRR765 pKa = 11.84 KK766 pKa = 9.42 CVVYY770 pKa = 9.29 PIKK773 pKa = 9.15 WTSHH777 pKa = 5.42 LLIFLLAATRR787 pKa = 11.84 KK788 pKa = 8.96 GLQRR792 pKa = 11.84 ATGIVNEE799 pKa = 4.41 RR800 pKa = 11.84 IGWEE804 pKa = 4.05 DD805 pKa = 3.68 EE806 pKa = 3.92 RR807 pKa = 11.84 PLAVRR812 pKa = 11.84 RR813 pKa = 11.84 RR814 pKa = 11.84 VVPIQKK820 pKa = 9.92 YY821 pKa = 10.22 ALPLLMALVTGSYY834 pKa = 10.74 ACTEE838 pKa = 3.97 MAIASSKK845 pKa = 9.87 MIKK848 pKa = 10.09 CQTEE852 pKa = 4.21 GGSTNCKK859 pKa = 10.16 ASGSVLLKK867 pKa = 10.54 AGPIGSEE874 pKa = 3.62 ACLVLKK880 pKa = 10.64 GSQEE884 pKa = 3.99 DD885 pKa = 3.43 QKK887 pKa = 11.78 KK888 pKa = 9.61 FISVKK893 pKa = 8.99 TLSSEE898 pKa = 4.45 LICLEE903 pKa = 4.56 GQDD906 pKa = 4.28 FWTGLYY912 pKa = 9.7 IPKK915 pKa = 9.81 CLSSRR920 pKa = 11.84 RR921 pKa = 11.84 CHH923 pKa = 6.98 LVGGCTGDD931 pKa = 5.05 SCQKK935 pKa = 9.49 WNNDD939 pKa = 3.06 HH940 pKa = 7.22 VSGEE944 pKa = 4.19 FKK946 pKa = 11.1 NLVDD950 pKa = 4.1 NEE952 pKa = 3.83 RR953 pKa = 11.84 MYY955 pKa = 11.31 EE956 pKa = 3.94 NRR958 pKa = 11.84 CFEE961 pKa = 3.9 QCGGLGCGCFNIQASCLFVHH981 pKa = 7.27 AEE983 pKa = 3.93 LEE985 pKa = 4.59 SVKK988 pKa = 10.72 KK989 pKa = 9.54 EE990 pKa = 3.94 AIRR993 pKa = 11.84 AFSCVDD999 pKa = 2.77 WVHH1002 pKa = 7.16 RR1003 pKa = 11.84 LTLEE1007 pKa = 4.39 VKK1009 pKa = 8.9 DD1010 pKa = 3.69 TQGNVEE1016 pKa = 4.41 KK1017 pKa = 11.09 VVLNDD1022 pKa = 3.43 MNTKK1026 pKa = 8.5 MFPWGSITLALDD1038 pKa = 3.55 AEE1040 pKa = 5.1 GMTSSNPFTFLQNRR1054 pKa = 11.84 EE1055 pKa = 3.88 GGFALVDD1062 pKa = 3.58 EE1063 pKa = 5.37 PFPNVPRR1070 pKa = 11.84 RR1071 pKa = 11.84 GQIGEE1076 pKa = 3.98 IRR1078 pKa = 11.84 CSNEE1082 pKa = 2.96 AAALSAHH1089 pKa = 6.7 KK1090 pKa = 10.5 SCVRR1094 pKa = 11.84 IQDD1097 pKa = 3.98 TINYY1101 pKa = 7.54 RR1102 pKa = 11.84 PHH1104 pKa = 6.35 MDD1106 pKa = 3.75 SVEE1109 pKa = 4.19 CTANLIDD1116 pKa = 5.13 PFTAFLKK1123 pKa = 10.93 GSLPQTRR1130 pKa = 11.84 GGMTFASSIDD1140 pKa = 3.6 GKK1142 pKa = 8.33 TVQAMASRR1150 pKa = 11.84 SVKK1153 pKa = 10.52 AQVSLSLDD1161 pKa = 3.47 EE1162 pKa = 4.53 YY1163 pKa = 11.36 DD1164 pKa = 5.09 FSFLSTVKK1172 pKa = 10.77 DD1173 pKa = 3.84 CTASFANMSGCYY1185 pKa = 9.43 SCNEE1189 pKa = 3.84 GAKK1192 pKa = 9.24 VCIRR1196 pKa = 11.84 AKK1198 pKa = 8.98 VTQSTTYY1205 pKa = 9.66 IAHH1208 pKa = 6.46 SKK1210 pKa = 10.64 DD1211 pKa = 3.53 FSINMALEE1219 pKa = 4.32 LRR1221 pKa = 11.84 PSTEE1225 pKa = 4.51 DD1226 pKa = 3.25 YY1227 pKa = 11.1 CQILHH1232 pKa = 6.62 FSKK1235 pKa = 10.63 PLIEE1239 pKa = 4.05 EE1240 pKa = 4.34 TMSYY1244 pKa = 9.21 TCGGPDD1250 pKa = 3.03 KK1251 pKa = 11.06 TMVISGHH1258 pKa = 6.86 LIAVDD1263 pKa = 3.44 VTTGTNTTGGDD1274 pKa = 3.62 STVVNPSEE1282 pKa = 5.03 DD1283 pKa = 3.29 SWSLFGWFSGLVDD1296 pKa = 3.43 WLGGPFKK1303 pKa = 10.97 ALIKK1307 pKa = 10.28 IGGYY1311 pKa = 9.47 ILGAVIVIALLALALRR1327 pKa = 11.84 LSVPLIIKK1335 pKa = 8.38 SFRR1338 pKa = 11.84 KK1339 pKa = 8.17 KK1340 pKa = 9.22 TNN1342 pKa = 2.91
Molecular weight: 147.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.526
IPC2_protein 6.693
IPC_protein 6.59
Toseland 6.478
ProMoST 7.161
Dawson 7.176
Bjellqvist 7.512
Wikipedia 7.117
Rodwell 7.19
Grimsley 6.532
Solomon 7.176
Lehninger 7.19
Nozaki 7.863
DTASelect 7.454
Thurlkill 7.497
EMBOSS 7.483
Sillero 7.717
Patrickios 3.999
IPC_peptide 7.19
IPC2_peptide 7.117
IPC2.peptide.svr19 6.895
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3339
1342
1997
1669.5
187.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.05 ± 0.077
2.636 ± 1.052
5.78 ± 0.024
7.008 ± 0.899
4.373 ± 0.499
6.11 ± 0.641
2.576 ± 0.019
6.259 ± 0.385
7.008 ± 0.002
8.835 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.815 ± 0.247
3.474 ± 0.144
3.384 ± 0.018
3.414 ± 0.163
5.241 ± 0.527
8.985 ± 0.232
5.69 ± 0.625
6.289 ± 0.282
1.228 ± 0.106
2.845 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here