Sulfolobus virus-like particle SSV2

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6UG89|Q6UG89_SSV2 ORF 328 OS=Sulfolobus virus-like particle SSV2 OX=244590 PE=4 SV=1
MM1 pKa = 7.5GPGPSVDD8 pKa = 3.62LYY10 pKa = 10.51LWTYY14 pKa = 10.19NGPPVDD20 pKa = 4.37LWVLDD25 pKa = 4.13HH26 pKa = 6.54QWTSISGPIMDD37 pKa = 4.92HH38 pKa = 5.86QWTTSPSFFFRR49 pKa = 11.84TIEE52 pKa = 3.95EE53 pKa = 4.13FLPSSFNFFTDD64 pKa = 2.9ILLEE68 pKa = 4.11

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6UG61|Q6UG61_SSV2 ORF 83 OS=Sulfolobus virus-like particle SSV2 OX=244590 PE=4 SV=1
MM1 pKa = 7.76AEE3 pKa = 4.25GGKK6 pKa = 10.26DD7 pKa = 3.69MRR9 pKa = 11.84WMTNGLTSPKK19 pKa = 9.92NIRR22 pKa = 11.84RR23 pKa = 11.84NSPRR27 pKa = 11.84FRR29 pKa = 11.84YY30 pKa = 9.7NYY32 pKa = 10.19YY33 pKa = 10.78DD34 pKa = 3.83YY35 pKa = 10.62LTSLQKK41 pKa = 9.63TKK43 pKa = 11.05AQIRR47 pKa = 11.84EE48 pKa = 4.47LNTKK52 pKa = 9.88PEE54 pKa = 3.66IHH56 pKa = 6.3RR57 pKa = 11.84VVLSHH62 pKa = 7.06LSYY65 pKa = 10.86HH66 pKa = 5.91RR67 pKa = 11.84LSRR70 pKa = 11.84KK71 pKa = 8.38SFCFISIFDD80 pKa = 4.13RR81 pKa = 11.84LLHH84 pKa = 6.24GSVPP88 pKa = 3.64

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

4979

48

809

150.9

17.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.86 ± 0.291

0.522 ± 0.179

3.274 ± 0.481

5.563 ± 1.071

4.78 ± 0.298

5.664 ± 0.623

1.346 ± 0.289

7.994 ± 0.386

6.909 ± 0.993

11.749 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.27

5.543 ± 0.628

3.937 ± 0.383

3.595 ± 0.44

3.716 ± 0.715

7.471 ± 0.718

6.447 ± 0.726

7.17 ± 0.472

1.567 ± 0.242

5.644 ± 0.645
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski