Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
Average proteome isoelectric point is 7.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5H3V1|Q5H3V1_XANOR Polyphosphate-selective porin O OS=Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85) OX=291331 GN=oprO PE=4 SV=1
MM1 pKa = 7.27 GWDD4 pKa = 3.34 NVAPPLQDD12 pKa = 2.79 RR13 pKa = 11.84 DD14 pKa = 3.87 YY15 pKa = 11.18 PMSEE19 pKa = 4.15 TSTTTYY25 pKa = 8.42 RR26 pKa = 11.84 TWMCVVCGFLYY37 pKa = 10.74 HH38 pKa = 6.64 EE39 pKa = 5.21 ADD41 pKa = 5.04 GIPEE45 pKa = 3.97 EE46 pKa = 5.35 GIAPGTRR53 pKa = 11.84 WQDD56 pKa = 3.42 VPDD59 pKa = 3.38 TWTCPDD65 pKa = 3.75 CGVTKK70 pKa = 10.56 EE71 pKa = 4.09 DD72 pKa = 3.9 FEE74 pKa = 4.44 MVEE77 pKa = 3.85 IDD79 pKa = 3.32
Molecular weight: 9.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.784
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.554
ProMoST 3.846
Dawson 3.745
Bjellqvist 3.961
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.872
Patrickios 0.477
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|Q5GU19|Q5GU19_XANOR Exodeoxyribonuclease III OS=Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85) OX=291331 GN=xthA1 PE=3 SV=1
MM1 pKa = 7.16 RR2 pKa = 11.84 VAWRR6 pKa = 11.84 GGRR9 pKa = 11.84 NAAGSTNLRR18 pKa = 11.84 RR19 pKa = 11.84 SFGAHH24 pKa = 5.74 ARR26 pKa = 11.84 TSTGLPHH33 pKa = 6.55 KK34 pKa = 10.43 QQ35 pKa = 2.92
Molecular weight: 3.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.437
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.281
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.121
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4382
0
4382
1408507
30
2941
321.4
35.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.697 ± 0.052
1.157 ± 0.014
5.485 ± 0.028
4.718 ± 0.031
3.092 ± 0.022
8.131 ± 0.038
2.684 ± 0.026
3.919 ± 0.026
2.859 ± 0.031
10.57 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.019
2.534 ± 0.026
5.417 ± 0.026
4.657 ± 0.029
8.127 ± 0.042
5.606 ± 0.033
5.093 ± 0.034
7.201 ± 0.028
1.601 ± 0.017
2.224 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here