Clostridium phage phiCD506

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Sherbrookevirus; Clostridioides virus phiCD506

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8WJ39|A0A0A8WJ39_9CAUD Uncharacterized protein OS=Clostridium phage phiCD506 OX=1582155 GN=PHICD506_20033 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 6.33NITEE6 pKa = 4.1QLKK9 pKa = 8.43EE10 pKa = 4.2TIVDD14 pKa = 3.48EE15 pKa = 5.29LYY17 pKa = 10.77DD18 pKa = 3.45IEE20 pKa = 4.53TNEE23 pKa = 4.39GCHH26 pKa = 6.11EE27 pKa = 4.73DD28 pKa = 3.88YY29 pKa = 11.11IEE31 pKa = 5.27DD32 pKa = 4.14YY33 pKa = 9.7EE34 pKa = 4.53TEE36 pKa = 3.96LDD38 pKa = 4.09FYY40 pKa = 11.31LSNVKK45 pKa = 10.32FGTYY49 pKa = 7.67EE50 pKa = 4.06VYY52 pKa = 10.83VKK54 pKa = 9.64EE55 pKa = 4.3YY56 pKa = 10.7CSNNYY61 pKa = 9.76DD62 pKa = 3.25ISISDD67 pKa = 3.53EE68 pKa = 4.02LAFEE72 pKa = 4.84IMDD75 pKa = 4.59DD76 pKa = 4.6LIGKK80 pKa = 9.42IKK82 pKa = 10.79DD83 pKa = 3.55NNN85 pKa = 3.57

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8WID1|A0A0A8WID1_9CAUD Uncharacterized protein OS=Clostridium phage phiCD506 OX=1582155 GN=PHICD506_20040 PE=4 SV=1
MM1 pKa = 7.39GRR3 pKa = 11.84LEE5 pKa = 3.89RR6 pKa = 11.84SKK8 pKa = 10.98KK9 pKa = 9.45KK10 pKa = 10.47RR11 pKa = 11.84EE12 pKa = 3.74NKK14 pKa = 9.71FNIVKK19 pKa = 10.27KK20 pKa = 9.83VFSFILLLLNIILAVLRR37 pKa = 11.84ILKK40 pKa = 9.66EE41 pKa = 3.83LL42 pKa = 3.73

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

9704

42

717

183.1

20.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.245 ± 0.329

1.041 ± 0.145

6.173 ± 0.278

9.13 ± 0.538

4.019 ± 0.208

5.338 ± 0.498

1.02 ± 0.151

9.326 ± 0.249

10.686 ± 0.355

8.522 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.223

6.801 ± 0.419

2.03 ± 0.167

2.484 ± 0.183

3.37 ± 0.214

5.956 ± 0.367

5.493 ± 0.348

5.627 ± 0.254

0.938 ± 0.124

4.225 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski