Amino acid dipepetide frequency for Paenibacillus sp. NFR01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.466AlaAla: 12.466 ± 0.173
0.733AlaCys: 0.733 ± 0.023
5.058AlaAsp: 5.058 ± 0.07
7.12AlaGlu: 7.12 ± 0.09
3.532AlaPhe: 3.532 ± 0.052
8.932AlaGly: 8.932 ± 0.11
1.47AlaHis: 1.47 ± 0.034
5.039AlaIle: 5.039 ± 0.07
4.465AlaLys: 4.465 ± 0.058
9.716AlaLeu: 9.716 ± 0.09
2.431AlaMet: 2.431 ± 0.041
2.635AlaAsn: 2.635 ± 0.046
3.459AlaPro: 3.459 ± 0.055
2.938AlaGln: 2.938 ± 0.05
4.067AlaArg: 4.067 ± 0.055
5.39AlaSer: 5.39 ± 0.077
4.091AlaThr: 4.091 ± 0.084
7.612AlaVal: 7.612 ± 0.074
1.074AlaTrp: 1.074 ± 0.03
2.964AlaTyr: 2.964 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.573CysAla: 0.573 ± 0.021
0.108CysCys: 0.108 ± 0.009
0.372CysAsp: 0.372 ± 0.014
0.411CysGlu: 0.411 ± 0.016
0.296CysPhe: 0.296 ± 0.013
0.821CysGly: 0.821 ± 0.026
0.167CysHis: 0.167 ± 0.011
0.478CysIle: 0.478 ± 0.018
0.276CysLys: 0.276 ± 0.015
0.756CysLeu: 0.756 ± 0.026
0.191CysMet: 0.191 ± 0.009
0.264CysAsn: 0.264 ± 0.014
0.351CysPro: 0.351 ± 0.015
0.19CysGln: 0.19 ± 0.01
0.507CysArg: 0.507 ± 0.02
0.505CysSer: 0.505 ± 0.017
0.386CysThr: 0.386 ± 0.017
0.38CysVal: 0.38 ± 0.018
0.093CysTrp: 0.093 ± 0.007
0.255CysTyr: 0.255 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
4.401AspAla: 4.401 ± 0.06
0.359AspCys: 0.359 ± 0.016
2.351AspAsp: 2.351 ± 0.041
3.628AspGlu: 3.628 ± 0.056
2.133AspPhe: 2.133 ± 0.046
4.302AspGly: 4.302 ± 0.066
1.004AspHis: 1.004 ± 0.027
3.388AspIle: 3.388 ± 0.052
2.608AspLys: 2.608 ± 0.045
4.858AspLeu: 4.858 ± 0.055
1.285AspMet: 1.285 ± 0.029
1.841AspAsn: 1.841 ± 0.036
2.428AspPro: 2.428 ± 0.044
1.521AspGln: 1.521 ± 0.03
2.677AspArg: 2.677 ± 0.043
2.846AspSer: 2.846 ± 0.046
2.615AspThr: 2.615 ± 0.046
3.25AspVal: 3.25 ± 0.05
0.828AspTrp: 0.828 ± 0.023
2.107AspTyr: 2.107 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
6.874GluAla: 6.874 ± 0.093
0.404GluCys: 0.404 ± 0.017
3.111GluAsp: 3.111 ± 0.045
5.271GluGlu: 5.271 ± 0.071
2.131GluPhe: 2.131 ± 0.039
4.695GluGly: 4.695 ± 0.067
1.536GluHis: 1.536 ± 0.033
4.143GluIle: 4.143 ± 0.066
3.312GluLys: 3.312 ± 0.053
7.486GluLeu: 7.486 ± 0.087
1.998GluMet: 1.998 ± 0.036
2.222GluAsn: 2.222 ± 0.037
2.464GluPro: 2.464 ± 0.045
3.317GluGln: 3.317 ± 0.055
4.178GluArg: 4.178 ± 0.065
3.27GluSer: 3.27 ± 0.046
3.358GluThr: 3.358 ± 0.046
4.36GluVal: 4.36 ± 0.066
0.867GluTrp: 0.867 ± 0.024
2.0GluTyr: 2.0 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.75PheAla: 3.75 ± 0.056
0.353PheCys: 0.353 ± 0.017
2.034PheAsp: 2.034 ± 0.038
2.144PheGlu: 2.144 ± 0.043
1.735PhePhe: 1.735 ± 0.042
3.373PheGly: 3.373 ± 0.057
0.814PheHis: 0.814 ± 0.027
2.588PheIle: 2.588 ± 0.048
1.858PheLys: 1.858 ± 0.039
3.61PheLeu: 3.61 ± 0.061
1.129PheMet: 1.129 ± 0.026
1.455PheAsn: 1.455 ± 0.034
1.534PhePro: 1.534 ± 0.034
1.19PheGln: 1.19 ± 0.028
2.181PheArg: 2.181 ± 0.039
2.784PheSer: 2.784 ± 0.051
2.532PheThr: 2.532 ± 0.053
2.698PheVal: 2.698 ± 0.046
0.525PheTrp: 0.525 ± 0.019
1.419PheTyr: 1.419 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
6.846GlyAla: 6.846 ± 0.097
0.723GlyCys: 0.723 ± 0.021
3.965GlyAsp: 3.965 ± 0.056
5.034GlyGlu: 5.034 ± 0.066
3.344GlyPhe: 3.344 ± 0.052
6.835GlyGly: 6.835 ± 0.095
1.583GlyHis: 1.583 ± 0.035
5.728GlyIle: 5.728 ± 0.072
4.591GlyLys: 4.591 ± 0.061
8.019GlyLeu: 8.019 ± 0.079
2.505GlyMet: 2.505 ± 0.046
2.965GlyAsn: 2.965 ± 0.053
2.207GlyPro: 2.207 ± 0.043
2.875GlyGln: 2.875 ± 0.043
4.098GlyArg: 4.098 ± 0.051
5.164GlySer: 5.164 ± 0.074
4.836GlyThr: 4.836 ± 0.078
5.489GlyVal: 5.489 ± 0.058
1.119GlyTrp: 1.119 ± 0.028
3.11GlyTyr: 3.11 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
1.656HisAla: 1.656 ± 0.035
0.183HisCys: 0.183 ± 0.012
0.875HisAsp: 0.875 ± 0.022
1.206HisGlu: 1.206 ± 0.03
0.959HisPhe: 0.959 ± 0.026
1.624HisGly: 1.624 ± 0.038
0.518HisHis: 0.518 ± 0.021
1.232HisIle: 1.232 ± 0.028
0.787HisLys: 0.787 ± 0.019
2.048HisLeu: 2.048 ± 0.056
0.536HisMet: 0.536 ± 0.019
0.651HisAsn: 0.651 ± 0.021
1.296HisPro: 1.296 ± 0.029
0.648HisGln: 0.648 ± 0.019
1.096HisArg: 1.096 ± 0.029
1.136HisSer: 1.136 ± 0.027
1.067HisThr: 1.067 ± 0.025
1.294HisVal: 1.294 ± 0.028
0.302HisTrp: 0.302 ± 0.015
0.822HisTyr: 0.822 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.152IleAla: 6.152 ± 0.07
0.527IleCys: 0.527 ± 0.017
3.31IleAsp: 3.31 ± 0.044
3.609IleGlu: 3.609 ± 0.052
2.221IlePhe: 2.221 ± 0.042
5.152IleGly: 5.152 ± 0.068
1.333IleHis: 1.333 ± 0.031
3.644IleIle: 3.644 ± 0.064
2.639IleLys: 2.639 ± 0.044
5.571IleLeu: 5.571 ± 0.075
1.492IleMet: 1.492 ± 0.031
2.106IleAsn: 2.106 ± 0.045
2.914IlePro: 2.914 ± 0.045
2.08IleGln: 2.08 ± 0.036
3.54IleArg: 3.54 ± 0.046
4.034IleSer: 4.034 ± 0.058
3.662IleThr: 3.662 ± 0.05
4.743IleVal: 4.743 ± 0.067
0.621IleTrp: 0.621 ± 0.022
1.929IleTyr: 1.929 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
4.539LysAla: 4.539 ± 0.061
0.228LysCys: 0.228 ± 0.013
2.755LysAsp: 2.755 ± 0.046
3.728LysGlu: 3.728 ± 0.065
1.375LysPhe: 1.375 ± 0.031
3.358LysGly: 3.358 ± 0.053
0.913LysHis: 0.913 ± 0.025
2.786LysIle: 2.786 ± 0.041
2.647LysLys: 2.647 ± 0.048
5.402LysLeu: 5.402 ± 0.073
1.385LysMet: 1.385 ± 0.029
1.83LysAsn: 1.83 ± 0.035
2.162LysPro: 2.162 ± 0.04
2.047LysGln: 2.047 ± 0.041
2.651LysArg: 2.651 ± 0.049
2.595LysSer: 2.595 ± 0.045
2.703LysThr: 2.703 ± 0.045
3.468LysVal: 3.468 ± 0.055
0.628LysTrp: 0.628 ± 0.018
1.655LysTyr: 1.655 ± 0.038
0.0LysXaa: 0.0 ± 0.0
Leu
9.744LeuAla: 9.744 ± 0.095
0.818LeuCys: 0.818 ± 0.024
5.204LeuAsp: 5.204 ± 0.054
6.68LeuGlu: 6.68 ± 0.08
4.343LeuPhe: 4.343 ± 0.068
7.633LeuGly: 7.633 ± 0.073
2.151LeuHis: 2.151 ± 0.04
6.103LeuIle: 6.103 ± 0.074
5.15LeuLys: 5.15 ± 0.059
12.023LeuLeu: 12.023 ± 0.13
2.625LeuMet: 2.625 ± 0.044
3.7LeuAsn: 3.7 ± 0.05
4.958LeuPro: 4.958 ± 0.056
4.056LeuGln: 4.056 ± 0.062
5.765LeuArg: 5.765 ± 0.071
7.079LeuSer: 7.079 ± 0.069
5.745LeuThr: 5.745 ± 0.066
6.239LeuVal: 6.239 ± 0.069
1.08LeuTrp: 1.08 ± 0.031
3.266LeuTyr: 3.266 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.312MetAla: 2.312 ± 0.041
0.152MetCys: 0.152 ± 0.009
1.461MetAsp: 1.461 ± 0.032
1.775MetGlu: 1.775 ± 0.03
0.993MetPhe: 0.993 ± 0.027
1.774MetGly: 1.774 ± 0.034
0.455MetHis: 0.455 ± 0.018
1.723MetIle: 1.723 ± 0.039
1.79MetLys: 1.79 ± 0.033
3.014MetLeu: 3.014 ± 0.052
0.816MetMet: 0.816 ± 0.029
1.418MetAsn: 1.418 ± 0.032
1.159MetPro: 1.159 ± 0.034
0.942MetGln: 0.942 ± 0.026
1.26MetArg: 1.26 ± 0.03
1.759MetSer: 1.759 ± 0.035
1.542MetThr: 1.542 ± 0.03
1.668MetVal: 1.668 ± 0.035
0.209MetTrp: 0.209 ± 0.011
0.733MetTyr: 0.733 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.116AsnAla: 3.116 ± 0.047
0.231AsnCys: 0.231 ± 0.012
1.749AsnAsp: 1.749 ± 0.036
2.197AsnGlu: 2.197 ± 0.04
1.319AsnPhe: 1.319 ± 0.029
3.148AsnGly: 3.148 ± 0.048
0.759AsnHis: 0.759 ± 0.023
2.227AsnIle: 2.227 ± 0.043
1.681AsnLys: 1.681 ± 0.037
3.251AsnLeu: 3.251 ± 0.049
0.915AsnMet: 0.915 ± 0.023
1.616AsnAsn: 1.616 ± 0.043
1.888AsnPro: 1.888 ± 0.034
1.225AsnGln: 1.225 ± 0.029
1.972AsnArg: 1.972 ± 0.039
1.922AsnSer: 1.922 ± 0.042
1.923AsnThr: 1.923 ± 0.041
2.52AsnVal: 2.52 ± 0.05
0.482AsnTrp: 0.482 ± 0.021
1.328AsnTyr: 1.328 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
4.414ProAla: 4.414 ± 0.065
0.234ProCys: 0.234 ± 0.011
2.669ProAsp: 2.669 ± 0.042
3.885ProGlu: 3.885 ± 0.058
1.77ProPhe: 1.77 ± 0.035
3.586ProGly: 3.586 ± 0.054
0.806ProHis: 0.806 ± 0.022
1.955ProIle: 1.955 ± 0.04
1.711ProLys: 1.711 ± 0.042
4.465ProLeu: 4.465 ± 0.055
0.912ProMet: 0.912 ± 0.023
1.321ProAsn: 1.321 ± 0.032
1.425ProPro: 1.425 ± 0.036
1.676ProGln: 1.676 ± 0.035
1.598ProArg: 1.598 ± 0.037
2.383ProSer: 2.383 ± 0.041
1.782ProThr: 1.782 ± 0.051
3.645ProVal: 3.645 ± 0.049
0.518ProTrp: 0.518 ± 0.019
1.528ProTyr: 1.528 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.531GlnAla: 3.531 ± 0.057
0.191GlnCys: 0.191 ± 0.011
1.613GlnAsp: 1.613 ± 0.031
2.602GlnGlu: 2.602 ± 0.048
1.305GlnPhe: 1.305 ± 0.028
2.694GlnGly: 2.694 ± 0.041
0.704GlnHis: 0.704 ± 0.025
2.28GlnIle: 2.28 ± 0.035
1.73GlnLys: 1.73 ± 0.034
3.806GlnLeu: 3.806 ± 0.05
1.107GlnMet: 1.107 ± 0.026
1.215GlnAsn: 1.215 ± 0.029
1.619GlnPro: 1.619 ± 0.035
1.656GlnGln: 1.656 ± 0.043
1.967GlnArg: 1.967 ± 0.039
2.047GlnSer: 2.047 ± 0.035
1.942GlnThr: 1.942 ± 0.04
2.298GlnVal: 2.298 ± 0.036
0.455GlnTrp: 0.455 ± 0.02
1.237GlnTyr: 1.237 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
3.726ArgAla: 3.726 ± 0.054
0.397ArgCys: 0.397 ± 0.018
2.421ArgAsp: 2.421 ± 0.046
4.112ArgGlu: 4.112 ± 0.065
2.234ArgPhe: 2.234 ± 0.039
3.579ArgGly: 3.579 ± 0.053
1.183ArgHis: 1.183 ± 0.033
3.549ArgIle: 3.549 ± 0.047
3.039ArgLys: 3.039 ± 0.045
5.933ArgLeu: 5.933 ± 0.073
1.678ArgMet: 1.678 ± 0.034
1.865ArgAsn: 1.865 ± 0.036
1.901ArgPro: 1.901 ± 0.042
2.192ArgGln: 2.192 ± 0.041
3.394ArgArg: 3.394 ± 0.059
3.375ArgSer: 3.375 ± 0.049
2.711ArgThr: 2.711 ± 0.042
3.078ArgVal: 3.078 ± 0.038
0.662ArgTrp: 0.662 ± 0.021
1.988ArgTyr: 1.988 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
5.817SerAla: 5.817 ± 0.084
0.406SerCys: 0.406 ± 0.016
2.841SerAsp: 2.841 ± 0.042
3.438SerGlu: 3.438 ± 0.052
2.878SerPhe: 2.878 ± 0.047
6.261SerGly: 6.261 ± 0.069
1.136SerHis: 1.136 ± 0.025
3.749SerIle: 3.749 ± 0.06
2.73SerLys: 2.73 ± 0.041
6.332SerLeu: 6.332 ± 0.067
1.586SerMet: 1.586 ± 0.027
1.919SerAsn: 1.919 ± 0.038
2.593SerPro: 2.593 ± 0.044
1.86SerGln: 1.86 ± 0.038
3.348SerArg: 3.348 ± 0.046
3.934SerSer: 3.934 ± 0.061
2.895SerThr: 2.895 ± 0.056
4.425SerVal: 4.425 ± 0.067
0.764SerTrp: 0.764 ± 0.022
2.091SerTyr: 2.091 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
5.591ThrAla: 5.591 ± 0.079
0.309ThrCys: 0.309 ± 0.013
2.734ThrAsp: 2.734 ± 0.046
3.218ThrGlu: 3.218 ± 0.053
2.232ThrPhe: 2.232 ± 0.043
5.273ThrGly: 5.273 ± 0.108
0.967ThrHis: 0.967 ± 0.024
3.128ThrIle: 3.128 ± 0.045
2.069ThrLys: 2.069 ± 0.043
5.773ThrLeu: 5.773 ± 0.066
1.238ThrMet: 1.238 ± 0.026
1.613ThrAsn: 1.613 ± 0.04
2.846ThrPro: 2.846 ± 0.054
1.414ThrGln: 1.414 ± 0.03
2.37ThrArg: 2.37 ± 0.037
3.087ThrSer: 3.087 ± 0.054
2.729ThrThr: 2.729 ± 0.057
4.838ThrVal: 4.838 ± 0.084
0.572ThrTrp: 0.572 ± 0.02
1.753ThrTyr: 1.753 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
5.763ValAla: 5.763 ± 0.066
0.615ValCys: 0.615 ± 0.021
3.489ValAsp: 3.489 ± 0.054
4.001ValGlu: 4.001 ± 0.059
2.864ValPhe: 2.864 ± 0.05
4.364ValGly: 4.364 ± 0.061
1.451ValHis: 1.451 ± 0.032
4.701ValIle: 4.701 ± 0.057
3.63ValLys: 3.63 ± 0.062
7.612ValLeu: 7.612 ± 0.079
1.888ValMet: 1.888 ± 0.038
2.796ValAsn: 2.796 ± 0.053
3.13ValPro: 3.13 ± 0.046
2.552ValGln: 2.552 ± 0.041
3.589ValArg: 3.589 ± 0.05
4.723ValSer: 4.723 ± 0.058
4.619ValThr: 4.619 ± 0.082
4.83ValVal: 4.83 ± 0.065
0.802ValTrp: 0.802 ± 0.023
2.403ValTyr: 2.403 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.889TrpAla: 0.889 ± 0.026
0.113TrpCys: 0.113 ± 0.009
0.605TrpAsp: 0.605 ± 0.021
0.754TrpGlu: 0.754 ± 0.023
0.494TrpPhe: 0.494 ± 0.018
0.854TrpGly: 0.854 ± 0.028
0.255TrpHis: 0.255 ± 0.013
0.82TrpIle: 0.82 ± 0.022
0.638TrpLys: 0.638 ± 0.019
1.363TrpLeu: 1.363 ± 0.03
0.38TrpMet: 0.38 ± 0.015
0.686TrpAsn: 0.686 ± 0.019
0.373TrpPro: 0.373 ± 0.015
0.486TrpGln: 0.486 ± 0.018
0.661TrpArg: 0.661 ± 0.019
0.816TrpSer: 0.816 ± 0.025
0.672TrpThr: 0.672 ± 0.022
0.768TrpVal: 0.768 ± 0.026
0.178TrpTrp: 0.178 ± 0.012
0.382TrpTyr: 0.382 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.035TyrAla: 3.035 ± 0.044
0.297TyrCys: 0.297 ± 0.015
1.813TyrAsp: 1.813 ± 0.034
2.183TyrGlu: 2.183 ± 0.037
1.548TyrPhe: 1.548 ± 0.035
2.778TyrGly: 2.778 ± 0.041
0.732TyrHis: 0.732 ± 0.022
2.017TyrIle: 2.017 ± 0.036
1.5TyrLys: 1.5 ± 0.029
3.325TyrLeu: 3.325 ± 0.055
0.856TyrMet: 0.856 ± 0.024
1.354TyrAsn: 1.354 ± 0.034
1.575TyrPro: 1.575 ± 0.034
1.136TyrGln: 1.136 ± 0.029
2.098TyrArg: 2.098 ± 0.038
2.152TyrSer: 2.152 ± 0.041
1.945TyrThr: 1.945 ± 0.039
2.221TyrVal: 2.221 ± 0.041
0.406TyrTrp: 0.406 ± 0.017
1.36TyrTyr: 1.36 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4792 proteins (1577750 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski