Sclerophthora macrospora virus B
Average proteome isoelectric point is 7.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9WTA1|Q9WTA1_9VIRU Polyprotein OS=Sclerophthora macrospora virus B OX=75914 PE=4 SV=1
MM1 pKa = 7.42 EE2 pKa = 6.21 PKK4 pKa = 9.27 TQVNYY9 pKa = 11.13 AEE11 pKa = 4.53 VVGGRR16 pKa = 11.84 YY17 pKa = 8.67 NRR19 pKa = 11.84 PAEE22 pKa = 3.99 VRR24 pKa = 11.84 AMRR27 pKa = 11.84 FLASNLYY34 pKa = 9.88 GAGIVFDD41 pKa = 3.96 ATEE44 pKa = 4.43 GGAKK48 pKa = 10.09 CFTSPPPHH56 pKa = 6.66 PASLRR61 pKa = 11.84 FCSVRR66 pKa = 11.84 GMPALAVCQTQGWWASFVLKK86 pKa = 10.51 SLPFPARR93 pKa = 11.84 WLIFQFLNYY102 pKa = 8.76 CWEE105 pKa = 4.0 WVEE108 pKa = 3.96 LQDD111 pKa = 4.46 RR112 pKa = 11.84 SAVFTATTWTLLPMAKK128 pKa = 9.43 RR129 pKa = 11.84 FMGPGLEE136 pKa = 4.09 YY137 pKa = 10.61 PEE139 pKa = 5.73 IGEE142 pKa = 4.29 DD143 pKa = 2.92 WFDD146 pKa = 5.55 RR147 pKa = 11.84 IIDD150 pKa = 3.74 SPRR153 pKa = 11.84 YY154 pKa = 9.28 RR155 pKa = 11.84 GWEE158 pKa = 3.81 VVPQVSYY165 pKa = 10.9 LYY167 pKa = 10.68 QNVGNCCLSYY177 pKa = 10.42 SAHH180 pKa = 5.82 EE181 pKa = 4.6 KK182 pKa = 9.81 IVAFVTEE189 pKa = 4.95 LYY191 pKa = 10.75 CRR193 pKa = 11.84 FSTYY197 pKa = 10.78 GIYY200 pKa = 10.67 LSARR204 pKa = 11.84 TLRR207 pKa = 11.84 QLSHH211 pKa = 4.89 VHH213 pKa = 6.26 HH214 pKa = 6.86 IKK216 pKa = 10.84 YY217 pKa = 9.23 MVMLQLVRR225 pKa = 11.84 SVPVARR231 pKa = 11.84 ALSTGDD237 pKa = 3.23 AWDD240 pKa = 3.97 LSLVFARR247 pKa = 11.84 FLEE250 pKa = 4.47 WEE252 pKa = 4.34 RR253 pKa = 11.84 RR254 pKa = 11.84 WSLSLLDD261 pKa = 5.99 LIAPYY266 pKa = 11.12 LNFAWRR272 pKa = 11.84 DD273 pKa = 3.74 CVSPTLVKK281 pKa = 10.08 TDD283 pKa = 3.2 VSLGMSCSKK292 pKa = 9.64 GTLVTFPSTLSLEE305 pKa = 4.72 FSQGLKK311 pKa = 10.67 FNVFEE316 pKa = 4.51 PSEE319 pKa = 4.23 LCCEE323 pKa = 4.33 EE324 pKa = 3.93 RR325 pKa = 11.84 HH326 pKa = 6.23 HH327 pKa = 6.9 PLLTVSMCLAYY338 pKa = 10.73 VLLWYY343 pKa = 10.21 LIIQLVIISADD354 pKa = 3.52 VVLDD358 pKa = 3.61 ASKK361 pKa = 10.68 YY362 pKa = 8.09 ICEE365 pKa = 4.37 TSLHH369 pKa = 5.06 VCQRR373 pKa = 11.84 FATLLSSLLRR383 pKa = 11.84 WYY385 pKa = 10.09 RR386 pKa = 11.84 LKK388 pKa = 10.22 IDD390 pKa = 3.5 RR391 pKa = 11.84 SLVRR395 pKa = 11.84 DD396 pKa = 3.54 SRR398 pKa = 11.84 VVTSCSGEE406 pKa = 3.89 APPVYY411 pKa = 10.05 RR412 pKa = 11.84 LVSKK416 pKa = 10.85 GPEE419 pKa = 3.93 AVDD422 pKa = 3.48 SLEE425 pKa = 3.57 MSMRR429 pKa = 11.84 GSEE432 pKa = 3.66 IRR434 pKa = 11.84 TSVAIPGCLYY444 pKa = 10.57 LAVVHH449 pKa = 7.0 DD450 pKa = 4.47 SDD452 pKa = 5.2 VIFIGMAFRR461 pKa = 11.84 YY462 pKa = 9.44 KK463 pKa = 10.64 GYY465 pKa = 10.62 LVTAQHH471 pKa = 6.81 NILAMSSAPGKK482 pKa = 10.79 YY483 pKa = 8.06 YY484 pKa = 10.7 VLPFRR489 pKa = 11.84 PGKK492 pKa = 10.27 DD493 pKa = 3.2 SEE495 pKa = 4.34 FCYY498 pKa = 10.6 LDD500 pKa = 3.31 QEE502 pKa = 4.54 RR503 pKa = 11.84 MIEE506 pKa = 4.08 LTSVMLHH513 pKa = 5.97 NDD515 pKa = 2.68 IVSEE519 pKa = 4.09 EE520 pKa = 3.92 FQGYY524 pKa = 9.47 DD525 pKa = 3.18 VAIIPIAAAAWSCLGVKK542 pKa = 10.12 SLLHH546 pKa = 7.26 ADD548 pKa = 3.68 ATWGINVTLYY558 pKa = 11.14 GLEE561 pKa = 3.97 KK562 pKa = 9.26 TGKK565 pKa = 9.88 RR566 pKa = 11.84 KK567 pKa = 9.0 LQRR570 pKa = 11.84 SLGTIRR576 pKa = 11.84 EE577 pKa = 4.33 DD578 pKa = 3.89 KK579 pKa = 10.49 EE580 pKa = 4.6 SPLHH584 pKa = 4.99 SVYY587 pKa = 11.19 YY588 pKa = 10.02 NASTLRR594 pKa = 11.84 GWSGSPVLNGHH605 pKa = 5.9 KK606 pKa = 9.97 RR607 pKa = 11.84 VVALHH612 pKa = 6.78 CGTNGQVNRR621 pKa = 11.84 GLNFAFVRR629 pKa = 11.84 FIIEE633 pKa = 3.37 IHH635 pKa = 6.5 EE636 pKa = 4.34 INTRR640 pKa = 11.84 EE641 pKa = 4.07 SSTDD645 pKa = 3.08 SYY647 pKa = 11.37 EE648 pKa = 4.57 DD649 pKa = 3.97 EE650 pKa = 4.19 IEE652 pKa = 3.97 KK653 pKa = 10.72 SFRR656 pKa = 11.84 TRR658 pKa = 11.84 GGYY661 pKa = 9.8 LSRR664 pKa = 11.84 RR665 pKa = 11.84 QEE667 pKa = 3.89 EE668 pKa = 4.61 LEE670 pKa = 3.86 VEE672 pKa = 4.13 RR673 pKa = 11.84 RR674 pKa = 11.84 VDD676 pKa = 3.25 EE677 pKa = 5.08 GIAVYY682 pKa = 10.19 KK683 pKa = 10.28 RR684 pKa = 11.84 YY685 pKa = 9.59 IVSQSSRR692 pKa = 11.84 GIISFCDD699 pKa = 2.88 SDD701 pKa = 4.05 YY702 pKa = 11.39 VDD704 pKa = 3.67 RR705 pKa = 11.84 QRR707 pKa = 11.84 ATHH710 pKa = 6.05 TATTNVVEE718 pKa = 5.37 QVFDD722 pKa = 4.61 DD723 pKa = 4.05 NLEE726 pKa = 4.01 SSYY729 pKa = 11.35 SPKK732 pKa = 10.41 CIQRR736 pKa = 11.84 CRR738 pKa = 11.84 TGEE741 pKa = 3.98 AEE743 pKa = 4.04 KK744 pKa = 11.2 SEE746 pKa = 4.49 DD747 pKa = 3.68 LSSAEE752 pKa = 4.26 QLFDD756 pKa = 3.64 YY757 pKa = 10.88 HH758 pKa = 8.62 DD759 pKa = 4.58 GDD761 pKa = 4.01 PFVASRR767 pKa = 11.84 VTTRR771 pKa = 11.84 LSAPHH776 pKa = 5.56 YY777 pKa = 9.01 KK778 pKa = 10.17 GRR780 pKa = 11.84 PEE782 pKa = 4.59 RR783 pKa = 11.84 YY784 pKa = 9.23 DD785 pKa = 3.21 EE786 pKa = 4.94 HH787 pKa = 8.47 VDD789 pKa = 3.59 LSRR792 pKa = 11.84 AKK794 pKa = 10.26 EE795 pKa = 3.79 LGYY798 pKa = 10.86 DD799 pKa = 3.54 PEE801 pKa = 4.54 EE802 pKa = 4.96 YY803 pKa = 11.04 GMPRR807 pKa = 11.84 ATDD810 pKa = 3.17 RR811 pKa = 11.84 TSAIKK816 pKa = 10.34 RR817 pKa = 11.84 SAASLSKK824 pKa = 10.72 SLEE827 pKa = 3.88 DD828 pKa = 5.16 AIRR831 pKa = 11.84 TSKK834 pKa = 9.62 FQKK837 pKa = 9.77 PSEE840 pKa = 4.36 DD841 pKa = 3.32 LRR843 pKa = 11.84 SAAVSIMMDD852 pKa = 3.4 TLSSLRR858 pKa = 11.84 YY859 pKa = 8.36 PVDD862 pKa = 3.31 GSGVTRR868 pKa = 11.84 DD869 pKa = 4.23 KK870 pKa = 10.89 IASQLNSSAVGDD882 pKa = 3.91 GRR884 pKa = 11.84 SPGLPYY890 pKa = 10.68 ASEE893 pKa = 4.36 FPTNRR898 pKa = 11.84 DD899 pKa = 3.36 LLSKK903 pKa = 10.51 FSVDD907 pKa = 3.16 EE908 pKa = 4.26 LADD911 pKa = 4.68 LIYY914 pKa = 10.58 TKK916 pKa = 10.62 YY917 pKa = 10.53 RR918 pKa = 11.84 EE919 pKa = 4.51 DD920 pKa = 3.11 RR921 pKa = 11.84 WEE923 pKa = 3.89 QPSINFLKK931 pKa = 10.42 IEE933 pKa = 3.97 PTKK936 pKa = 10.78 VEE938 pKa = 4.59 KK939 pKa = 10.65 LDD941 pKa = 3.58 QGLDD945 pKa = 3.08 RR946 pKa = 11.84 CVQAVGLDD954 pKa = 3.31 TQLYY958 pKa = 7.66 FRR960 pKa = 11.84 CHH962 pKa = 6.13 FGALADD968 pKa = 3.84 VASANYY974 pKa = 9.43 RR975 pKa = 11.84 KK976 pKa = 10.15 SPVMQGWSPLKK987 pKa = 10.61 PGDD990 pKa = 3.24 GHH992 pKa = 6.15 YY993 pKa = 10.24 LYY995 pKa = 11.18 NVITRR1000 pKa = 11.84 NSNRR1004 pKa = 11.84 KK1005 pKa = 8.52 ILEE1008 pKa = 3.81 YY1009 pKa = 10.38 DD1010 pKa = 3.64 GKK1012 pKa = 10.21 AFEE1015 pKa = 4.78 YY1016 pKa = 10.31 VAHH1019 pKa = 6.03 TAEE1022 pKa = 4.86 AYY1024 pKa = 10.85 SDD1026 pKa = 3.73 VTDD1029 pKa = 4.23 VIIGLSTPAVGANVDD1044 pKa = 3.91 KK1045 pKa = 10.88 VRR1047 pKa = 11.84 EE1048 pKa = 4.2 WRR1050 pKa = 11.84 SEE1052 pKa = 3.59 AQRR1055 pKa = 11.84 FMMRR1059 pKa = 11.84 VGEE1062 pKa = 4.18 SGYY1065 pKa = 11.24 LLADD1069 pKa = 3.39 GTLVSKK1075 pKa = 10.8 RR1076 pKa = 11.84 SPFVLSSGRR1085 pKa = 11.84 WDD1087 pKa = 3.34 TFLRR1091 pKa = 11.84 NSLTGYY1097 pKa = 8.16 YY1098 pKa = 9.32 WLIIGLLDD1106 pKa = 4.29 AGYY1109 pKa = 8.15 TADD1112 pKa = 5.05 DD1113 pKa = 3.73 IKK1115 pKa = 11.48 AKK1117 pKa = 10.46 FLIKK1121 pKa = 10.72 VGGDD1125 pKa = 3.56 DD1126 pKa = 4.53 VILSVPSNFDD1136 pKa = 3.22 TQTFTSALGRR1146 pKa = 11.84 YY1147 pKa = 7.66 GMKK1150 pKa = 9.2 IHH1152 pKa = 6.72 KK1153 pKa = 9.99 VKK1155 pKa = 10.76 LKK1157 pKa = 10.52 PITDD1161 pKa = 3.67 DD1162 pKa = 4.42 FEE1164 pKa = 4.42 FFSWKK1169 pKa = 9.68 FSKK1172 pKa = 10.62 NSRR1175 pKa = 11.84 GLVQWTPTRR1184 pKa = 11.84 FSKK1187 pKa = 10.81 HH1188 pKa = 5.81 LEE1190 pKa = 3.74 NFFNTKK1196 pKa = 10.24 HH1197 pKa = 6.38 EE1198 pKa = 4.65 DD1199 pKa = 2.9 RR1200 pKa = 11.84 HH1201 pKa = 5.2 QALVSHH1207 pKa = 6.22 MMNWVHH1213 pKa = 6.91 SEE1215 pKa = 3.27 PHH1217 pKa = 5.82 YY1218 pKa = 11.09 NFFRR1222 pKa = 11.84 EE1223 pKa = 3.65 IYY1225 pKa = 8.02 MRR1227 pKa = 11.84 GRR1229 pKa = 11.84 EE1230 pKa = 4.32 SEE1232 pKa = 3.88 PDD1234 pKa = 2.96 IYY1236 pKa = 10.6 EE1237 pKa = 3.8 LSKK1240 pKa = 10.63 IPEE1243 pKa = 3.97 RR1244 pKa = 11.84 RR1245 pKa = 11.84 DD1246 pKa = 3.02 LLFYY1250 pKa = 11.08 LRR1252 pKa = 11.84 GNEE1255 pKa = 4.6 SVIKK1259 pKa = 10.41 DD1260 pKa = 3.55 WGSVGAFVSLASAAVLKK1277 pKa = 11.14 NNN1279 pKa = 4.11
Molecular weight: 145.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.467
IPC2_protein 6.59
IPC_protein 6.605
Toseland 6.649
ProMoST 7.073
Dawson 7.073
Bjellqvist 7.161
Wikipedia 7.059
Rodwell 7.073
Grimsley 6.751
Solomon 7.088
Lehninger 7.088
Nozaki 7.439
DTASelect 7.424
Thurlkill 7.454
EMBOSS 7.439
Sillero 7.512
Patrickios 4.088
IPC_peptide 7.088
IPC2_peptide 7.073
IPC2.peptide.svr19 6.895
Protein with the highest isoelectric point:
>tr|Q9WTA1|Q9WTA1_9VIRU Polyprotein OS=Sclerophthora macrospora virus B OX=75914 PE=4 SV=1
MM1 pKa = 7.14 TKK3 pKa = 10.66 KK4 pKa = 10.21 NVKK7 pKa = 8.41 TSGAIVVYY15 pKa = 10.23 KK16 pKa = 9.88 PQTRR20 pKa = 11.84 LPPQNKK26 pKa = 9.19 KK27 pKa = 10.58 KK28 pKa = 10.25 NRR30 pKa = 11.84 KK31 pKa = 8.29 KK32 pKa = 10.36 KK33 pKa = 10.3 GRR35 pKa = 11.84 VGPGKK40 pKa = 10.53 SDD42 pKa = 3.14 MLACYY47 pKa = 9.26 RR48 pKa = 11.84 ASLSAPFSITAQGARR63 pKa = 11.84 VPDD66 pKa = 4.29 MYY68 pKa = 11.21 SCPTVTRR75 pKa = 11.84 HH76 pKa = 4.26 ITKK79 pKa = 10.36 SFTVVTNSAGEE90 pKa = 3.89 ADD92 pKa = 4.47 LVVLPSAFHH101 pKa = 6.32 HH102 pKa = 6.03 AVSPRR107 pKa = 11.84 NNIPSGVTWSTLSGATAANALVFTSPGQLANSLVNYY143 pKa = 9.65 RR144 pKa = 11.84 IVGYY148 pKa = 7.74 GVRR151 pKa = 11.84 IFGVASMTNTAGRR164 pKa = 11.84 AVIATVPIASWINDD178 pKa = 3.03 KK179 pKa = 9.49 TASVGGQVSNAVNAAANVAGTLVAYY204 pKa = 7.58 GVPVTGAYY212 pKa = 10.18 VDD214 pKa = 3.81 IASLPSLPNTVGVSMMNLSEE234 pKa = 4.24 RR235 pKa = 11.84 QMIITPKK242 pKa = 8.87 ITGPEE247 pKa = 3.37 AFVFNEE253 pKa = 4.19 TTDD256 pKa = 3.34 NAIGFNIVDD265 pKa = 3.6 QTSVSFVASGDD276 pKa = 3.37 ASYY279 pKa = 11.38 LRR281 pKa = 11.84 VAGFEE286 pKa = 4.16 GVVIGLSGCPPNVGVLEE303 pKa = 4.3 VEE305 pKa = 4.36 LVYY308 pKa = 10.73 HH309 pKa = 6.93 LEE311 pKa = 4.26 GQPTITNNNIVGDD324 pKa = 4.17 SPEE327 pKa = 4.31 SFCAPVSFLNTLQAIAKK344 pKa = 7.77 TPTFKK349 pKa = 10.59 QGLKK353 pKa = 10.25 VVGNSIYY360 pKa = 10.39 PGLGTLASRR369 pKa = 11.84 YY370 pKa = 9.18
Molecular weight: 38.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.883
IPC2_protein 9.033
IPC_protein 8.96
Toseland 9.56
ProMoST 9.399
Dawson 9.853
Bjellqvist 9.589
Wikipedia 10.028
Rodwell 10.131
Grimsley 9.926
Solomon 9.882
Lehninger 9.838
Nozaki 9.663
DTASelect 9.545
Thurlkill 9.677
EMBOSS 9.999
Sillero 9.794
Patrickios 4.927
IPC_peptide 9.867
IPC2_peptide 8.302
IPC2.peptide.svr19 7.975
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1649
370
1279
824.5
91.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.58 ± 1.376
1.698 ± 0.316
4.912 ± 1.269
5.276 ± 1.455
4.306 ± 0.405
6.671 ± 1.15
2.062 ± 0.502
4.791 ± 0.315
4.488 ± 0.055
8.914 ± 1.381
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.941 ± 0.025
3.76 ± 1.534
4.73 ± 0.899
2.668 ± 0.018
6.367 ± 1.46
9.46 ± 0.277
5.761 ± 1.339
8.611 ± 1.679
1.698 ± 0.592
4.306 ± 0.682
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here