Pepo aphid-borne yellows virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172ZQ18|A0A172ZQ18_9LUTE Readthrough protein OS=Pepo aphid-borne yellows virus OX=1462681 PE=3 SV=1
MM1 pKa = 7.35NMLFVLFFSLSLSLCGATSPVSGTALIQPQPLNFNSSFDD40 pKa = 3.82GLLSAEE46 pKa = 4.3WVSPPPEE53 pKa = 3.98VRR55 pKa = 11.84WIEE58 pKa = 4.24GTLQWPPQDD67 pKa = 4.16QEE69 pKa = 4.34EE70 pKa = 4.61TSCDD74 pKa = 3.55YY75 pKa = 10.99SYY77 pKa = 9.79HH78 pKa = 6.6TIFQKK83 pKa = 10.63LQEE86 pKa = 4.88NILEE90 pKa = 4.58DD91 pKa = 4.8GKK93 pKa = 11.32KK94 pKa = 9.92LWLGGHH100 pKa = 5.08QQLTNFSTNIYY111 pKa = 9.39NLSRR115 pKa = 11.84ALASDD120 pKa = 3.91ACTLLIDD127 pKa = 5.01SIIFLWSFILWGVVWCVFRR146 pKa = 11.84FVKK149 pKa = 10.08TYY151 pKa = 9.3FLRR154 pKa = 11.84ALTIAFLVACTACMAKK170 pKa = 10.14CLRR173 pKa = 11.84ALSGLLAPFPRR184 pKa = 11.84LLLSVINFIYY194 pKa = 10.59SVFLGMLTPFQSSKK208 pKa = 7.96TRR210 pKa = 11.84KK211 pKa = 6.9TNCWEE216 pKa = 4.06KK217 pKa = 10.49QLKK220 pKa = 8.65GWNSFEE226 pKa = 4.68IPMNPPSKK234 pKa = 10.42SVIEE238 pKa = 3.82ITYY241 pKa = 10.88DD242 pKa = 3.22NGSHH246 pKa = 6.69CGYY249 pKa = 10.65AVTVQTYY256 pKa = 10.31KK257 pKa = 9.72KK258 pKa = 7.59TASGAFEE265 pKa = 4.39SAVLTSLHH273 pKa = 6.5CLDD276 pKa = 4.94GGDD279 pKa = 3.74EE280 pKa = 4.26KK281 pKa = 11.34FLFSRR286 pKa = 11.84RR287 pKa = 11.84TGQGIPLAQCTTLAVWKK304 pKa = 10.79SLDD307 pKa = 3.72LCLLTPPANFQSTLGVKK324 pKa = 9.11PCPMVCMDD332 pKa = 5.22RR333 pKa = 11.84LAGCDD338 pKa = 3.0ASFFRR343 pKa = 11.84LNKK346 pKa = 9.17EE347 pKa = 4.56GAWSMTNCKK356 pKa = 10.14LLGAQKK362 pKa = 10.28DD363 pKa = 3.81GSVAVLVNSQPGHH376 pKa = 5.88SGSPFFNGKK385 pKa = 8.99NVLGVLRR392 pKa = 11.84GPDD395 pKa = 3.44PDD397 pKa = 3.87NNFNIMNPIPAVEE410 pKa = 4.2GLTVPEE416 pKa = 4.54LRR418 pKa = 11.84FEE420 pKa = 4.37TTAPTGKK427 pKa = 10.45LFTDD431 pKa = 4.04DD432 pKa = 4.94NLMKK436 pKa = 10.72ALAEE440 pKa = 4.2EE441 pKa = 4.29YY442 pKa = 10.24TRR444 pKa = 11.84MKK446 pKa = 10.92NFVPASGKK454 pKa = 10.0AWADD458 pKa = 3.3YY459 pKa = 11.2DD460 pKa = 3.77SDD462 pKa = 3.87EE463 pKa = 4.95EE464 pKa = 4.39YY465 pKa = 11.22FEE467 pKa = 5.6AAAKK471 pKa = 10.41DD472 pKa = 3.49LAGPSTLQGNRR483 pKa = 11.84RR484 pKa = 11.84GQARR488 pKa = 11.84LPNQRR493 pKa = 11.84GPCRR497 pKa = 11.84LPEE500 pKa = 4.26NDD502 pKa = 4.4AGNDD506 pKa = 3.67GKK508 pKa = 10.81DD509 pKa = 4.03DD510 pKa = 3.57IQHH513 pKa = 6.46FSGGHH518 pKa = 5.34KK519 pKa = 9.66RR520 pKa = 11.84DD521 pKa = 3.8CGGEE525 pKa = 3.91AGRR528 pKa = 11.84KK529 pKa = 7.93AVPYY533 pKa = 8.63EE534 pKa = 3.74QASPEE539 pKa = 4.06EE540 pKa = 4.16EE541 pKa = 4.41PPVSSAKK548 pKa = 9.43QAEE551 pKa = 4.48DD552 pKa = 3.31FRR554 pKa = 11.84QYY556 pKa = 11.22FNSLYY561 pKa = 10.26KK562 pKa = 9.82WDD564 pKa = 3.53VCAPSSEE571 pKa = 4.25VPGFRR576 pKa = 11.84NCGHH580 pKa = 7.55LPQFYY585 pKa = 10.39RR586 pKa = 11.84PGKK589 pKa = 9.28KK590 pKa = 10.06AEE592 pKa = 4.25TEE594 pKa = 3.72WGKK597 pKa = 7.81TLTKK601 pKa = 10.07EE602 pKa = 4.09YY603 pKa = 9.06PALGAATRR611 pKa = 11.84GFGWPEE617 pKa = 3.79FGPAAEE623 pKa = 4.51LKK625 pKa = 10.44SLRR628 pKa = 11.84LQSARR633 pKa = 11.84WRR635 pKa = 11.84EE636 pKa = 3.97RR637 pKa = 11.84ASSATEE643 pKa = 3.69PCQSEE648 pKa = 4.49RR649 pKa = 11.84EE650 pKa = 4.06RR651 pKa = 11.84VIAEE655 pKa = 3.61LTEE658 pKa = 4.43RR659 pKa = 11.84YY660 pKa = 10.52SNVKK664 pKa = 9.96SCAPACARR672 pKa = 11.84QEE674 pKa = 4.17TLSWDD679 pKa = 3.61GFLEE683 pKa = 4.83DD684 pKa = 4.68IKK686 pKa = 11.28SAIPFLSLDD695 pKa = 3.51AGVGVPYY702 pKa = 10.2IAYY705 pKa = 8.32GKK707 pKa = 7.16PTLAQWVHH715 pKa = 6.69DD716 pKa = 4.41EE717 pKa = 4.23SLLPLVARR725 pKa = 11.84LAFDD729 pKa = 3.64RR730 pKa = 11.84LKK732 pKa = 11.17KK733 pKa = 9.25MSEE736 pKa = 4.02VSFEE740 pKa = 4.04ALSPEE745 pKa = 4.1EE746 pKa = 4.04LVQEE750 pKa = 4.59GLCDD754 pKa = 4.87PIRR757 pKa = 11.84VFVKK761 pKa = 10.78GEE763 pKa = 3.59PHH765 pKa = 6.19KK766 pKa = 10.54QSKK769 pKa = 10.21LDD771 pKa = 3.38EE772 pKa = 4.27GRR774 pKa = 11.84YY775 pKa = 8.96RR776 pKa = 11.84LIMSVSMVDD785 pKa = 3.26QLVARR790 pKa = 11.84VLFQNQNRR798 pKa = 11.84AEE800 pKa = 4.07IALWRR805 pKa = 11.84AIPSKK810 pKa = 10.48PGLGLSTDD818 pKa = 3.54EE819 pKa = 4.94QITDD823 pKa = 4.25FAQNLSQYY831 pKa = 9.23VRR833 pKa = 11.84EE834 pKa = 4.14SRR836 pKa = 11.84GDD838 pKa = 3.31IIEE841 pKa = 4.3RR842 pKa = 11.84WHH844 pKa = 6.26EE845 pKa = 4.1CVVPTDD851 pKa = 3.67CSGFDD856 pKa = 3.34WSVSSWMLEE865 pKa = 3.63DD866 pKa = 6.11DD867 pKa = 3.23IVVRR871 pKa = 11.84NNLTLDD877 pKa = 3.27NTEE880 pKa = 3.63LTKK883 pKa = 10.56RR884 pKa = 11.84LRR886 pKa = 11.84AAWLKK891 pKa = 10.89CIANSVLCLSDD902 pKa = 3.35GTLLAQEE909 pKa = 4.44FPGVQKK915 pKa = 10.71SGSYY919 pKa = 8.25NTSSSNSRR927 pKa = 11.84IRR929 pKa = 11.84VMASIYY935 pKa = 10.79AGASWCIAMGDD946 pKa = 3.91DD947 pKa = 4.33ALEE950 pKa = 4.7SSDD953 pKa = 4.02TDD955 pKa = 3.64LKK957 pKa = 11.25VYY959 pKa = 10.84SDD961 pKa = 4.52LGLKK965 pKa = 10.27VEE967 pKa = 4.53VSDD970 pKa = 3.87KK971 pKa = 11.68LEE973 pKa = 4.33FCSHH977 pKa = 6.49HH978 pKa = 6.35FKK980 pKa = 10.84SSSLAIPVNVGKK992 pKa = 9.06MLYY995 pKa = 10.42KK996 pKa = 10.55LIHH999 pKa = 6.81GYY1001 pKa = 9.62NIRR1004 pKa = 11.84PGCTSVEE1011 pKa = 4.23VIANYY1016 pKa = 9.49MSACYY1021 pKa = 9.94SVLNEE1026 pKa = 4.0LRR1028 pKa = 11.84HH1029 pKa = 5.31VPEE1032 pKa = 5.62AIPLVSACLFSSS1044 pKa = 3.92

Molecular weight: 115.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172ZPW1|A0A172ZPW1_9LUTE Serine protease OS=Pepo aphid-borne yellows virus OX=1462681 PE=4 SV=1
MM1 pKa = 6.72NTVAVRR7 pKa = 11.84SSNGTRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84PRR19 pKa = 11.84RR20 pKa = 11.84SARR23 pKa = 11.84RR24 pKa = 11.84VRR26 pKa = 11.84VAMVQAIGPAQRR38 pKa = 11.84GGRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84VNRR51 pKa = 11.84GGRR54 pKa = 11.84ARR56 pKa = 11.84GGRR59 pKa = 11.84QGEE62 pKa = 4.26TFVFSKK68 pKa = 11.12DD69 pKa = 3.51NLSGSSSGSITFGPSLSEE87 pKa = 4.04SPAFSSGILKK97 pKa = 10.26AYY99 pKa = 10.13HH100 pKa = 6.54EE101 pKa = 4.61YY102 pKa = 10.53KK103 pKa = 9.31ITMVKK108 pKa = 10.64LEE110 pKa = 4.9FISEE114 pKa = 4.18ASSTSSGSISYY125 pKa = 10.24EE126 pKa = 3.75LDD128 pKa = 3.23PHH130 pKa = 6.86CKK132 pKa = 10.08LSSLQSTVNKK142 pKa = 10.31FGITKK147 pKa = 10.11NGSRR151 pKa = 11.84SWTAKK156 pKa = 10.17FINGLEE162 pKa = 4.02WHH164 pKa = 7.33DD165 pKa = 3.56ATEE168 pKa = 4.09DD169 pKa = 3.41QFRR172 pKa = 11.84ILYY175 pKa = 9.14KK176 pKa = 10.93GNGSSSVAGSFKK188 pKa = 9.88ITIQCQVQNPKK199 pKa = 10.62

Molecular weight: 22.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3048

45

1044

435.4

48.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.185 ± 0.443

2.165 ± 0.549

4.724 ± 0.546

5.971 ± 0.382

4.462 ± 0.648

7.415 ± 0.544

1.739 ± 0.321

4.331 ± 0.321

4.954 ± 0.548

8.957 ± 1.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.297

4.626 ± 0.295

6.299 ± 0.463

3.707 ± 0.193

6.398 ± 1.067

10.039 ± 0.974

5.184 ± 0.385

5.381 ± 0.455

2.034 ± 0.183

2.657 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski