Amino acid dipepetide frequency for Burkholderiales bacterium PBB5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.879AlaAla: 22.879 ± 0.159	1.425AlaCys: 1.425 ± 0.026	8.06AlaAsp: 8.06 ± 0.077	6.933AlaGlu: 6.933 ± 0.069	3.883AlaPhe: 3.883 ± 0.05	13.038AlaGly: 13.038 ± 0.092	3.096AlaHis: 3.096 ± 0.047	4.475AlaIle: 4.475 ± 0.047	3.181AlaLys: 3.181 ± 0.056	18.955AlaLeu: 18.955 ± 0.161	3.618AlaMet: 3.618 ± 0.052	2.953AlaAsn: 2.953 ± 0.043	7.718AlaPro: 7.718 ± 0.081	7.538AlaGln: 7.538 ± 0.085	10.204AlaArg: 10.204 ± 0.09	7.191AlaSer: 7.191 ± 0.069	7.461AlaThr: 7.461 ± 0.07	10.596AlaVal: 10.596 ± 0.084	2.473AlaTrp: 2.473 ± 0.044	2.392AlaTyr: 2.392 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.272CysAla: 1.272 ± 0.027	0.111CysCys: 0.111 ± 0.008	0.54CysAsp: 0.54 ± 0.018	0.4CysGlu: 0.4 ± 0.015	0.287CysPhe: 0.287 ± 0.013	0.987CysGly: 0.987 ± 0.026	0.302CysHis: 0.302 ± 0.013	0.297CysIle: 0.297 ± 0.013	0.173CysLys: 0.173 ± 0.011	0.904CysLeu: 0.904 ± 0.025	0.184CysMet: 0.184 ± 0.01	0.222CysAsn: 0.222 ± 0.01	0.513CysPro: 0.513 ± 0.02	0.325CysGln: 0.325 ± 0.013	0.621CysArg: 0.621 ± 0.019	0.514CysSer: 0.514 ± 0.016	0.551CysThr: 0.551 ± 0.019	0.689CysVal: 0.689 ± 0.02	0.144CysTrp: 0.144 ± 0.009	0.173CysTyr: 0.173 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.946AspAla: 7.946 ± 0.069	0.431AspCys: 0.431 ± 0.016	2.947AspAsp: 2.947 ± 0.045	3.027AspGlu: 3.027 ± 0.042	1.844AspPhe: 1.844 ± 0.033	5.269AspGly: 5.269 ± 0.057	1.228AspHis: 1.228 ± 0.03	1.94AspIle: 1.94 ± 0.035	1.344AspLys: 1.344 ± 0.032	5.555AspLeu: 5.555 ± 0.064	1.002AspMet: 1.002 ± 0.022	1.177AspAsn: 1.177 ± 0.028	2.871AspPro: 2.871 ± 0.042	1.866AspGln: 1.866 ± 0.034	3.238AspArg: 3.238 ± 0.039	2.319AspSer: 2.319 ± 0.039	2.736AspThr: 2.736 ± 0.037	3.689AspVal: 3.689 ± 0.042	0.997AspTrp: 0.997 ± 0.027	1.135AspTyr: 1.135 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.674GluAla: 6.674 ± 0.07	0.333GluCys: 0.333 ± 0.014	1.672GluAsp: 1.672 ± 0.029	1.371GluGlu: 1.371 ± 0.03	1.336GluPhe: 1.336 ± 0.03	3.191GluGly: 3.191 ± 0.046	1.122GluHis: 1.122 ± 0.026	1.723GluIle: 1.723 ± 0.032	0.962GluLys: 0.962 ± 0.027	5.213GluLeu: 5.213 ± 0.067	0.927GluMet: 0.927 ± 0.022	0.796GluAsn: 0.796 ± 0.023	2.448GluPro: 2.448 ± 0.037	2.252GluGln: 2.252 ± 0.037	4.055GluArg: 4.055 ± 0.042	1.812GluSer: 1.812 ± 0.034	1.896GluThr: 1.896 ± 0.032	3.615GluVal: 3.615 ± 0.05	0.564GluTrp: 0.564 ± 0.018	0.651GluTyr: 0.651 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	4.074PheAla: 4.074 ± 0.05	0.339PheCys: 0.339 ± 0.014	2.385PheAsp: 2.385 ± 0.035	1.821PheGlu: 1.821 ± 0.035	1.059PhePhe: 1.059 ± 0.023	3.117PheGly: 3.117 ± 0.045	0.673PheHis: 0.673 ± 0.021	1.225PheIle: 1.225 ± 0.028	0.974PheLys: 0.974 ± 0.023	2.354PheLeu: 2.354 ± 0.036	0.646PheMet: 0.646 ± 0.019	1.053PheAsn: 1.053 ± 0.027	1.194PhePro: 1.194 ± 0.026	1.016PheGln: 1.016 ± 0.022	1.606PheArg: 1.606 ± 0.03	1.817PheSer: 1.817 ± 0.029	1.821PheThr: 1.821 ± 0.035	2.416PheVal: 2.416 ± 0.038	0.458PheTrp: 0.458 ± 0.016	0.664PheTyr: 0.664 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	10.78GlyAla: 10.78 ± 0.081	0.965GlyCys: 0.965 ± 0.023	4.257GlyAsp: 4.257 ± 0.052	3.854GlyGlu: 3.854 ± 0.053	3.117GlyPhe: 3.117 ± 0.039	7.61GlyGly: 7.61 ± 0.078	2.202GlyHis: 2.202 ± 0.037	3.256GlyIle: 3.256 ± 0.043	2.414GlyLys: 2.414 ± 0.042	10.686GlyLeu: 10.686 ± 0.087	2.073GlyMet: 2.073 ± 0.036	2.054GlyAsn: 2.054 ± 0.039	3.935GlyPro: 3.935 ± 0.052	4.509GlyGln: 4.509 ± 0.045	6.18GlyArg: 6.18 ± 0.057	4.671GlySer: 4.671 ± 0.063	4.845GlyThr: 4.845 ± 0.062	6.752GlyVal: 6.752 ± 0.062	1.778GlyTrp: 1.778 ± 0.034	2.078GlyTyr: 2.078 ± 0.039	0.002GlyXaa: 0.002 ± 0.001
His	3.235HisAla: 3.235 ± 0.042	0.326HisCys: 0.326 ± 0.013	1.241HisAsp: 1.241 ± 0.03	1.069HisGlu: 1.069 ± 0.025	0.882HisPhe: 0.882 ± 0.023	2.336HisGly: 2.336 ± 0.043	0.756HisHis: 0.756 ± 0.024	0.796HisIle: 0.796 ± 0.023	0.42HisLys: 0.42 ± 0.016	2.529HisLeu: 2.529 ± 0.034	0.408HisMet: 0.408 ± 0.013	0.503HisAsn: 0.503 ± 0.018	1.565HisPro: 1.565 ± 0.032	0.939HisGln: 0.939 ± 0.025	1.732HisArg: 1.732 ± 0.033	1.064HisSer: 1.064 ± 0.025	1.206HisThr: 1.206 ± 0.025	1.454HisVal: 1.454 ± 0.031	0.515HisTrp: 0.515 ± 0.017	0.566HisTyr: 0.566 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.099IleAla: 5.099 ± 0.059	0.272IleCys: 0.272 ± 0.013	2.759IleAsp: 2.759 ± 0.041	2.217IleGlu: 2.217 ± 0.038	0.854IlePhe: 0.854 ± 0.022	3.57IleGly: 3.57 ± 0.045	0.682IleHis: 0.682 ± 0.018	0.907IleIle: 0.907 ± 0.027	0.968IleLys: 0.968 ± 0.026	2.364IleLeu: 2.364 ± 0.041	0.443IleMet: 0.443 ± 0.016	1.153IleAsn: 1.153 ± 0.023	1.555IlePro: 1.555 ± 0.027	1.026IleGln: 1.026 ± 0.027	1.97IleArg: 1.97 ± 0.036	1.844IleSer: 1.844 ± 0.034	2.329IleThr: 2.329 ± 0.04	2.747IleVal: 2.747 ± 0.042	0.344IleTrp: 0.344 ± 0.013	0.589IleTyr: 0.589 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	3.439LysAla: 3.439 ± 0.059	0.113LysCys: 0.113 ± 0.008	1.189LysAsp: 1.189 ± 0.03	0.786LysGlu: 0.786 ± 0.023	0.634LysPhe: 0.634 ± 0.02	1.845LysGly: 1.845 ± 0.034	0.466LysHis: 0.466 ± 0.017	0.856LysIle: 0.856 ± 0.027	0.779LysLys: 0.779 ± 0.025	2.869LysLeu: 2.869 ± 0.041	0.48LysMet: 0.48 ± 0.014	0.562LysAsn: 0.562 ± 0.019	1.773LysPro: 1.773 ± 0.037	0.904LysGln: 0.904 ± 0.022	1.668LysArg: 1.668 ± 0.032	1.153LysSer: 1.153 ± 0.028	1.376LysThr: 1.376 ± 0.03	1.953LysVal: 1.953 ± 0.036	0.259LysTrp: 0.259 ± 0.012	0.438LysTyr: 0.438 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	19.387LeuAla: 19.387 ± 0.162	1.048LeuCys: 1.048 ± 0.025	6.206LeuAsp: 6.206 ± 0.066	3.847LeuGlu: 3.847 ± 0.051	3.001LeuPhe: 3.001 ± 0.044	10.049LeuGly: 10.049 ± 0.094	2.588LeuHis: 2.588 ± 0.04	3.484LeuIle: 3.484 ± 0.044	2.969LeuLys: 2.969 ± 0.049	12.707LeuLeu: 12.707 ± 0.129	2.624LeuMet: 2.624 ± 0.039	2.536LeuAsn: 2.536 ± 0.035	7.312LeuPro: 7.312 ± 0.073	5.543LeuGln: 5.543 ± 0.071	8.911LeuArg: 8.911 ± 0.085	6.117LeuSer: 6.117 ± 0.071	5.436LeuThr: 5.436 ± 0.067	8.765LeuVal: 8.765 ± 0.069	1.598LeuTrp: 1.598 ± 0.032	1.866LeuTyr: 1.866 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	3.438MetAla: 3.438 ± 0.041	0.148MetCys: 0.148 ± 0.009	1.002MetAsp: 1.002 ± 0.023	0.633MetGlu: 0.633 ± 0.02	0.563MetPhe: 0.563 ± 0.017	1.799MetGly: 1.799 ± 0.03	0.522MetHis: 0.522 ± 0.018	0.681MetIle: 0.681 ± 0.018	0.669MetLys: 0.669 ± 0.021	2.442MetLeu: 2.442 ± 0.036	0.426MetMet: 0.426 ± 0.018	0.649MetAsn: 0.649 ± 0.019	1.52MetPro: 1.52 ± 0.031	0.905MetGln: 0.905 ± 0.023	1.567MetArg: 1.567 ± 0.029	1.373MetSer: 1.373 ± 0.027	1.36MetThr: 1.36 ± 0.031	1.642MetVal: 1.642 ± 0.034	0.181MetTrp: 0.181 ± 0.01	0.279MetTyr: 0.279 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.182AsnAla: 3.182 ± 0.046	0.219AsnCys: 0.219 ± 0.012	1.131AsnAsp: 1.131 ± 0.027	0.887AsnGlu: 0.887 ± 0.022	0.815AsnPhe: 0.815 ± 0.023	2.081AsnGly: 2.081 ± 0.037	0.505AsnHis: 0.505 ± 0.016	0.82AsnIle: 0.82 ± 0.023	0.564AsnLys: 0.564 ± 0.02	2.496AsnLeu: 2.496 ± 0.036	0.394AsnMet: 0.394 ± 0.016	0.656AsnAsn: 0.656 ± 0.024	1.757AsnPro: 1.757 ± 0.03	0.867AsnGln: 0.867 ± 0.023	1.507AsnArg: 1.507 ± 0.026	0.97AsnSer: 0.97 ± 0.022	1.33AsnThr: 1.33 ± 0.031	1.608AsnVal: 1.608 ± 0.03	0.316AsnTrp: 0.316 ± 0.014	0.503AsnTyr: 0.503 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.286ProAla: 9.286 ± 0.082	0.385ProCys: 0.385 ± 0.014	3.309ProAsp: 3.309 ± 0.04	3.063ProGlu: 3.063 ± 0.043	1.664ProPhe: 1.664 ± 0.028	5.599ProGly: 5.599 ± 0.062	1.161ProHis: 1.161 ± 0.026	1.616ProIle: 1.616 ± 0.036	1.225ProLys: 1.225 ± 0.03	6.221ProLeu: 6.221 ± 0.065	1.356ProMet: 1.356 ± 0.027	1.092ProAsn: 1.092 ± 0.025	3.398ProPro: 3.398 ± 0.058	2.44ProGln: 2.44 ± 0.041	3.373ProArg: 3.373 ± 0.048	2.724ProSer: 2.724 ± 0.043	2.894ProThr: 2.894 ± 0.042	4.797ProVal: 4.797 ± 0.053	0.923ProTrp: 0.923 ± 0.026	1.053ProTyr: 1.053 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	8.245GlnAla: 8.245 ± 0.094	0.339GlnCys: 0.339 ± 0.013	1.588GlnAsp: 1.588 ± 0.031	1.062GlnGlu: 1.062 ± 0.024	1.217GlnPhe: 1.217 ± 0.025	3.632GlnGly: 3.632 ± 0.047	1.229GlnHis: 1.229 ± 0.026	1.591GlnIle: 1.591 ± 0.037	0.727GlnLys: 0.727 ± 0.022	5.242GlnLeu: 5.242 ± 0.079	0.889GlnMet: 0.889 ± 0.024	0.605GlnAsn: 0.605 ± 0.018	3.115GlnPro: 3.115 ± 0.041	2.439GlnGln: 2.439 ± 0.044	4.749GlnArg: 4.749 ± 0.063	1.681GlnSer: 1.681 ± 0.033	1.856GlnThr: 1.856 ± 0.036	3.753GlnVal: 3.753 ± 0.051	0.797GlnTrp: 0.797 ± 0.022	0.651GlnTyr: 0.651 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.052ArgAla: 9.052 ± 0.081	0.774ArgCys: 0.774 ± 0.022	3.489ArgAsp: 3.489 ± 0.044	3.311ArgGlu: 3.311 ± 0.044	2.674ArgPhe: 2.674 ± 0.036	4.979ArgGly: 4.979 ± 0.055	2.154ArgHis: 2.154 ± 0.041	2.824ArgIle: 2.824 ± 0.038	1.408ArgLys: 1.408 ± 0.03	9.282ArgLeu: 9.282 ± 0.087	1.727ArgMet: 1.727 ± 0.031	1.517ArgAsn: 1.517 ± 0.028	3.796ArgPro: 3.796 ± 0.054	3.767ArgGln: 3.767 ± 0.055	5.617ArgArg: 5.617 ± 0.066	3.623ArgSer: 3.623 ± 0.042	3.163ArgThr: 3.163 ± 0.04	5.225ArgVal: 5.225 ± 0.055	1.515ArgTrp: 1.515 ± 0.032	1.603ArgTyr: 1.603 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	7.244SerAla: 7.244 ± 0.076	0.442SerCys: 0.442 ± 0.017	2.347SerAsp: 2.347 ± 0.036	1.812SerGlu: 1.812 ± 0.031	1.851SerPhe: 1.851 ± 0.034	5.159SerGly: 5.159 ± 0.072	1.154SerHis: 1.154 ± 0.025	1.71SerIle: 1.71 ± 0.031	1.058SerLys: 1.058 ± 0.028	5.496SerLeu: 5.496 ± 0.064	0.999SerMet: 0.999 ± 0.02	1.176SerAsn: 1.176 ± 0.03	3.004SerPro: 3.004 ± 0.037	1.884SerGln: 1.884 ± 0.034	3.175SerArg: 3.175 ± 0.04	2.64SerSer: 2.64 ± 0.044	2.975SerThr: 2.975 ± 0.051	3.639SerVal: 3.639 ± 0.053	0.699SerTrp: 0.699 ± 0.02	1.032SerTyr: 1.032 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	7.277ThrAla: 7.277 ± 0.077	0.396ThrCys: 0.396 ± 0.017	2.567ThrAsp: 2.567 ± 0.041	2.184ThrGlu: 2.184 ± 0.036	1.345ThrPhe: 1.345 ± 0.027	5.009ThrGly: 5.009 ± 0.061	1.098ThrHis: 1.098 ± 0.021	1.678ThrIle: 1.678 ± 0.034	0.96ThrLys: 0.96 ± 0.025	6.848ThrLeu: 6.848 ± 0.06	1.055ThrMet: 1.055 ± 0.025	1.038ThrAsn: 1.038 ± 0.029	3.781ThrPro: 3.781 ± 0.045	1.922ThrGln: 1.922 ± 0.036	3.144ThrArg: 3.144 ± 0.038	2.504ThrSer: 2.504 ± 0.045	3.051ThrThr: 3.051 ± 0.054	4.547ThrVal: 4.547 ± 0.067	0.721ThrTrp: 0.721 ± 0.02	0.896ThrTyr: 0.896 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	11.204ValAla: 11.204 ± 0.089	0.762ValCys: 0.762 ± 0.023	4.218ValAsp: 4.218 ± 0.041	3.147ValGlu: 3.147 ± 0.042	2.445ValPhe: 2.445 ± 0.042	5.892ValGly: 5.892 ± 0.06	1.787ValHis: 1.787 ± 0.035	2.704ValIle: 2.704 ± 0.041	1.917ValLys: 1.917 ± 0.034	9.234ValLeu: 9.234 ± 0.086	1.876ValMet: 1.876 ± 0.031	2.003ValAsn: 2.003 ± 0.039	4.337ValPro: 4.337 ± 0.052	3.495ValGln: 3.495 ± 0.042	5.147ValArg: 5.147 ± 0.056	3.797ValSer: 3.797 ± 0.045	3.857ValThr: 3.857 ± 0.053	6.604ValVal: 6.604 ± 0.061	1.118ValTrp: 1.118 ± 0.025	1.381ValTyr: 1.381 ± 0.026	0.001ValXaa: 0.001 ± 0.001
Trp	1.968TrpAla: 1.968 ± 0.031	0.2TrpCys: 0.2 ± 0.011	0.651TrpAsp: 0.651 ± 0.019	0.408TrpGlu: 0.408 ± 0.017	0.523TrpPhe: 0.523 ± 0.019	1.109TrpGly: 1.109 ± 0.025	0.442TrpHis: 0.442 ± 0.016	0.524TrpIle: 0.524 ± 0.016	0.324TrpLys: 0.324 ± 0.013	2.473TrpLeu: 2.473 ± 0.043	0.364TrpMet: 0.364 ± 0.014	0.35TrpAsn: 0.35 ± 0.012	1.01TrpPro: 1.01 ± 0.025	0.992TrpGln: 0.992 ± 0.026	1.511TrpArg: 1.511 ± 0.037	0.736TrpSer: 0.736 ± 0.02	0.745TrpThr: 0.745 ± 0.021	1.153TrpVal: 1.153 ± 0.026	0.332TrpTrp: 0.332 ± 0.015	0.253TrpTyr: 0.253 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.387TyrAla: 2.387 ± 0.035	0.185TyrCys: 0.185 ± 0.01	1.003TyrAsp: 1.003 ± 0.023	0.779TyrGlu: 0.779 ± 0.02	0.721TyrPhe: 0.721 ± 0.021	1.747TyrGly: 1.747 ± 0.031	0.372TyrHis: 0.372 ± 0.015	0.586TyrIle: 0.586 ± 0.021	0.505TyrLys: 0.505 ± 0.018	2.125TyrLeu: 2.125 ± 0.036	0.31TyrMet: 0.31 ± 0.015	0.511TyrAsn: 0.511 ± 0.015	0.974TyrPro: 0.974 ± 0.023	0.782TyrGln: 0.782 ± 0.022	1.525TyrArg: 1.525 ± 0.028	0.949TyrSer: 0.949 ± 0.022	1.13TyrThr: 1.13 ± 0.027	1.278TyrVal: 1.278 ± 0.025	0.336TyrTrp: 0.336 ± 0.012	0.434TyrTyr: 0.434 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 8079 proteins (1874526 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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