Streptococcus phage Javan172

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ARI2|A0A4D6ARI2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan172 OX=2548021 GN=Javan172_0030 PE=4 SV=1
MM1 pKa = 7.93CDD3 pKa = 2.99VKK5 pKa = 11.08ALSEE9 pKa = 4.3FNGGEE14 pKa = 4.06VTIKK18 pKa = 10.43RR19 pKa = 11.84DD20 pKa = 3.53GEE22 pKa = 4.04NASYY26 pKa = 10.39VVPIEE31 pKa = 4.23RR32 pKa = 11.84VILMQSIGLKK42 pKa = 10.16DD43 pKa = 3.52KK44 pKa = 11.52NGVEE48 pKa = 4.24VFDD51 pKa = 4.41GDD53 pKa = 4.04IMFYY57 pKa = 10.65EE58 pKa = 4.15QDD60 pKa = 3.6CYY62 pKa = 11.28QYY64 pKa = 11.16TLVKK68 pKa = 10.15YY69 pKa = 10.66DD70 pKa = 3.48KK71 pKa = 10.94DD72 pKa = 3.19KK73 pKa = 11.45LAFVLYY79 pKa = 10.08DD80 pKa = 3.27GCEE83 pKa = 3.9RR84 pKa = 11.84LYY86 pKa = 11.19HH87 pKa = 5.78EE88 pKa = 4.62LWEE91 pKa = 4.37TGEE94 pKa = 4.36VIGNIYY100 pKa = 9.86EE101 pKa = 4.15NPEE104 pKa = 3.9LLEE107 pKa = 4.31SVEE110 pKa = 4.31EE111 pKa = 4.19

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ASW5|A0A4D6ASW5_9CAUD Integrase OS=Streptococcus phage Javan172 OX=2548021 GN=Javan172_0001 PE=3 SV=1
MM1 pKa = 7.89EE2 pKa = 4.94YY3 pKa = 10.82GNKK6 pKa = 9.42IFEE9 pKa = 4.64IYY11 pKa = 10.45NKK13 pKa = 9.36PFKK16 pKa = 10.45YY17 pKa = 10.16RR18 pKa = 11.84NSSSTNYY25 pKa = 10.37NKK27 pKa = 9.41VRR29 pKa = 11.84ASGIEE34 pKa = 3.94PNTKK38 pKa = 9.55FVVNKK43 pKa = 8.88TSNVNCAVYY52 pKa = 9.39PRR54 pKa = 11.84HH55 pKa = 6.17GSIEE59 pKa = 4.07KK60 pKa = 9.81VFYY63 pKa = 10.15WGDD66 pKa = 3.35RR67 pKa = 11.84KK68 pKa = 8.82ITQATAEE75 pKa = 4.24KK76 pKa = 9.87RR77 pKa = 11.84CGYY80 pKa = 10.49FKK82 pKa = 11.29GG83 pKa = 3.73

Molecular weight: 9.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

11548

39

1366

183.3

20.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.59 ± 0.707

0.623 ± 0.101

6.503 ± 0.313

8.027 ± 0.325

4.035 ± 0.237

6.131 ± 0.36

1.403 ± 0.14

7.179 ± 0.304

9.612 ± 0.383

8.478 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.186

5.516 ± 0.266

2.858 ± 0.209

3.905 ± 0.203

4.174 ± 0.286

5.975 ± 0.367

5.646 ± 0.281

5.932 ± 0.266

1.23 ± 0.105

3.533 ± 0.307
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski