Amino acid dipepetide frequency for Streptomyces phage Dubu

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.438AlaAla: 16.438 ± 1.87
0.909AlaCys: 0.909 ± 0.275
8.508AlaAsp: 8.508 ± 0.991
8.095AlaGlu: 8.095 ± 1.205
3.717AlaPhe: 3.717 ± 0.508
9.086AlaGly: 9.086 ± 0.813
2.148AlaHis: 2.148 ± 0.379
4.048AlaIle: 4.048 ± 0.699
4.626AlaLys: 4.626 ± 0.7
12.473AlaLeu: 12.473 ± 1.58
2.726AlaMet: 2.726 ± 0.567
2.726AlaAsn: 2.726 ± 0.492
6.36AlaPro: 6.36 ± 1.095
4.543AlaGln: 4.543 ± 0.627
9.086AlaArg: 9.086 ± 1.23
6.526AlaSer: 6.526 ± 0.926
7.6AlaThr: 7.6 ± 0.93
10.325AlaVal: 10.325 ± 1.116
3.387AlaTrp: 3.387 ± 0.543
3.139AlaTyr: 3.139 ± 0.503
0.0AlaXaa: 0.0 ± 0.0
Cys
0.909CysAla: 0.909 ± 0.267
0.083CysCys: 0.083 ± 0.081
0.413CysAsp: 0.413 ± 0.179
0.496CysGlu: 0.496 ± 0.224
0.33CysPhe: 0.33 ± 0.165
0.826CysGly: 0.826 ± 0.288
0.33CysHis: 0.33 ± 0.178
0.33CysIle: 0.33 ± 0.195
0.083CysLys: 0.083 ± 0.104
0.413CysLeu: 0.413 ± 0.166
0.083CysMet: 0.083 ± 0.078
0.0CysAsn: 0.0 ± 0.0
0.743CysPro: 0.743 ± 0.261
0.33CysGln: 0.33 ± 0.22
0.826CysArg: 0.826 ± 0.34
0.578CysSer: 0.578 ± 0.282
0.083CysThr: 0.083 ± 0.083
0.496CysVal: 0.496 ± 0.182
0.083CysTrp: 0.083 ± 0.076
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
9.995AspAla: 9.995 ± 1.064
0.578AspCys: 0.578 ± 0.237
4.543AspAsp: 4.543 ± 0.759
2.726AspGlu: 2.726 ± 0.557
2.148AspPhe: 2.148 ± 0.349
6.113AspGly: 6.113 ± 0.693
1.239AspHis: 1.239 ± 0.39
2.065AspIle: 2.065 ± 0.401
1.982AspLys: 1.982 ± 0.389
5.782AspLeu: 5.782 ± 0.575
1.735AspMet: 1.735 ± 0.41
1.569AspAsn: 1.569 ± 0.538
3.882AspPro: 3.882 ± 0.513
2.974AspGln: 2.974 ± 0.427
4.791AspArg: 4.791 ± 0.538
3.304AspSer: 3.304 ± 0.455
2.643AspThr: 2.643 ± 0.441
5.782AspVal: 5.782 ± 0.753
1.404AspTrp: 1.404 ± 0.362
1.735AspTyr: 1.735 ± 0.43
0.0AspXaa: 0.0 ± 0.0
Glu
8.095GluAla: 8.095 ± 1.239
0.496GluCys: 0.496 ± 0.178
4.708GluAsp: 4.708 ± 0.6
5.369GluGlu: 5.369 ± 1.037
1.652GluPhe: 1.652 ± 0.411
4.461GluGly: 4.461 ± 0.53
1.404GluHis: 1.404 ± 0.4
2.065GluIle: 2.065 ± 0.351
2.561GluLys: 2.561 ± 0.529
5.7GluLeu: 5.7 ± 0.519
0.826GluMet: 0.826 ± 0.254
0.661GluAsn: 0.661 ± 0.305
3.387GluPro: 3.387 ± 0.679
0.743GluGln: 0.743 ± 0.345
5.039GluArg: 5.039 ± 0.778
2.065GluSer: 2.065 ± 0.372
3.304GluThr: 3.304 ± 0.549
4.378GluVal: 4.378 ± 0.612
1.239GluTrp: 1.239 ± 0.307
1.404GluTyr: 1.404 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
3.139PheAla: 3.139 ± 0.517
0.083PheCys: 0.083 ± 0.087
2.148PheAsp: 2.148 ± 0.431
1.735PheGlu: 1.735 ± 0.352
0.661PhePhe: 0.661 ± 0.326
3.139PheGly: 3.139 ± 0.645
0.413PheHis: 0.413 ± 0.263
0.826PheIle: 0.826 ± 0.254
1.569PheLys: 1.569 ± 0.519
2.643PheLeu: 2.643 ± 0.426
0.826PheMet: 0.826 ± 0.284
0.578PheAsn: 0.578 ± 0.204
1.817PhePro: 1.817 ± 0.434
1.652PheGln: 1.652 ± 0.326
2.891PheArg: 2.891 ± 0.533
1.156PheSer: 1.156 ± 0.339
2.809PheThr: 2.809 ± 0.477
1.404PheVal: 1.404 ± 0.315
0.33PheTrp: 0.33 ± 0.15
0.991PheTyr: 0.991 ± 0.299
0.0PheXaa: 0.0 ± 0.0
Gly
7.434GlyAla: 7.434 ± 0.842
0.578GlyCys: 0.578 ± 0.224
4.874GlyAsp: 4.874 ± 0.683
4.461GlyGlu: 4.461 ± 0.373
3.304GlyPhe: 3.304 ± 0.708
6.278GlyGly: 6.278 ± 1.011
1.156GlyHis: 1.156 ± 0.279
3.222GlyIle: 3.222 ± 0.574
3.965GlyLys: 3.965 ± 0.527
7.765GlyLeu: 7.765 ± 0.902
2.396GlyMet: 2.396 ± 0.435
1.982GlyAsn: 1.982 ± 0.835
5.121GlyPro: 5.121 ± 0.719
4.048GlyGln: 4.048 ± 0.536
5.534GlyArg: 5.534 ± 0.634
5.369GlySer: 5.369 ± 0.862
6.443GlyThr: 6.443 ± 1.216
4.461GlyVal: 4.461 ± 0.758
1.652GlyTrp: 1.652 ± 0.457
1.817GlyTyr: 1.817 ± 0.584
0.0GlyXaa: 0.0 ± 0.0
His
2.313HisAla: 2.313 ± 0.397
0.083HisCys: 0.083 ± 0.087
1.239HisAsp: 1.239 ± 0.306
0.991HisGlu: 0.991 ± 0.257
0.909HisPhe: 0.909 ± 0.297
2.065HisGly: 2.065 ± 0.38
0.248HisHis: 0.248 ± 0.132
0.909HisIle: 0.909 ± 0.293
0.743HisLys: 0.743 ± 0.273
1.074HisLeu: 1.074 ± 0.24
0.165HisMet: 0.165 ± 0.114
0.413HisAsn: 0.413 ± 0.222
0.826HisPro: 0.826 ± 0.237
0.743HisGln: 0.743 ± 0.273
0.661HisArg: 0.661 ± 0.219
0.496HisSer: 0.496 ± 0.248
1.156HisThr: 1.156 ± 0.264
1.322HisVal: 1.322 ± 0.302
0.248HisTrp: 0.248 ± 0.131
0.165HisTyr: 0.165 ± 0.102
0.0HisXaa: 0.0 ± 0.0
Ile
3.387IleAla: 3.387 ± 0.736
0.33IleCys: 0.33 ± 0.141
2.396IleAsp: 2.396 ± 0.449
1.817IleGlu: 1.817 ± 0.315
0.496IlePhe: 0.496 ± 0.215
2.974IleGly: 2.974 ± 0.602
0.743IleHis: 0.743 ± 0.297
1.074IleIle: 1.074 ± 0.361
1.404IleLys: 1.404 ± 0.49
3.056IleLeu: 3.056 ± 0.529
0.248IleMet: 0.248 ± 0.168
0.661IleAsn: 0.661 ± 0.189
1.322IlePro: 1.322 ± 0.407
1.817IleGln: 1.817 ± 0.512
2.643IleArg: 2.643 ± 0.537
2.726IleSer: 2.726 ± 0.438
2.478IleThr: 2.478 ± 0.642
2.065IleVal: 2.065 ± 0.353
0.909IleTrp: 0.909 ± 0.29
0.496IleTyr: 0.496 ± 0.183
0.0IleXaa: 0.0 ± 0.0
Lys
6.113LysAla: 6.113 ± 1.028
0.248LysCys: 0.248 ± 0.146
2.396LysAsp: 2.396 ± 0.487
1.487LysGlu: 1.487 ± 0.37
0.991LysPhe: 0.991 ± 0.314
2.726LysGly: 2.726 ± 0.583
0.661LysHis: 0.661 ± 0.258
0.578LysIle: 0.578 ± 0.282
0.991LysLys: 0.991 ± 0.295
2.891LysLeu: 2.891 ± 0.545
0.991LysMet: 0.991 ± 0.282
0.578LysAsn: 0.578 ± 0.251
3.222LysPro: 3.222 ± 0.602
0.991LysGln: 0.991 ± 0.317
3.056LysArg: 3.056 ± 0.551
2.478LysSer: 2.478 ± 0.425
1.9LysThr: 1.9 ± 0.43
2.726LysVal: 2.726 ± 0.526
0.743LysTrp: 0.743 ± 0.216
0.743LysTyr: 0.743 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
12.721LeuAla: 12.721 ± 0.933
0.496LeuCys: 0.496 ± 0.218
5.617LeuAsp: 5.617 ± 0.735
5.947LeuGlu: 5.947 ± 0.832
1.652LeuPhe: 1.652 ± 0.458
6.113LeuGly: 6.113 ± 1.22
1.652LeuHis: 1.652 ± 0.403
1.982LeuIle: 1.982 ± 0.33
2.726LeuLys: 2.726 ± 0.521
5.121LeuLeu: 5.121 ± 0.727
2.561LeuMet: 2.561 ± 0.412
2.726LeuAsn: 2.726 ± 0.757
5.369LeuPro: 5.369 ± 0.611
2.561LeuGln: 2.561 ± 0.479
6.278LeuArg: 6.278 ± 0.677
4.791LeuSer: 4.791 ± 0.789
6.939LeuThr: 6.939 ± 0.821
7.517LeuVal: 7.517 ± 0.921
0.991LeuTrp: 0.991 ± 0.281
1.404LeuTyr: 1.404 ± 0.296
0.0LeuXaa: 0.0 ± 0.0
Met
3.882MetAla: 3.882 ± 0.506
0.083MetCys: 0.083 ± 0.104
0.826MetAsp: 0.826 ± 0.258
1.074MetGlu: 1.074 ± 0.218
0.743MetPhe: 0.743 ± 0.276
1.569MetGly: 1.569 ± 0.454
0.083MetHis: 0.083 ± 0.074
0.743MetIle: 0.743 ± 0.251
1.404MetLys: 1.404 ± 0.417
0.909MetLeu: 0.909 ± 0.278
0.413MetMet: 0.413 ± 0.218
0.909MetAsn: 0.909 ± 0.272
1.404MetPro: 1.404 ± 0.349
0.248MetGln: 0.248 ± 0.12
1.569MetArg: 1.569 ± 0.332
2.065MetSer: 2.065 ± 0.4
1.735MetThr: 1.735 ± 0.537
1.982MetVal: 1.982 ± 0.504
0.165MetTrp: 0.165 ± 0.119
0.248MetTyr: 0.248 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
2.809AsnAla: 2.809 ± 0.574
0.248AsnCys: 0.248 ± 0.143
1.487AsnAsp: 1.487 ± 0.402
0.413AsnGlu: 0.413 ± 0.218
0.413AsnPhe: 0.413 ± 0.198
3.635AsnGly: 3.635 ± 0.555
0.743AsnHis: 0.743 ± 0.284
0.661AsnIle: 0.661 ± 0.267
0.743AsnLys: 0.743 ± 0.275
1.982AsnLeu: 1.982 ± 0.489
0.661AsnMet: 0.661 ± 0.271
0.33AsnAsn: 0.33 ± 0.144
1.735AsnPro: 1.735 ± 0.383
0.661AsnGln: 0.661 ± 0.325
1.322AsnArg: 1.322 ± 0.336
1.074AsnSer: 1.074 ± 0.468
1.404AsnThr: 1.404 ± 0.733
1.817AsnVal: 1.817 ± 0.4
0.578AsnTrp: 0.578 ± 0.402
0.826AsnTyr: 0.826 ± 0.352
0.0AsnXaa: 0.0 ± 0.0
Pro
6.03ProAla: 6.03 ± 0.826
0.578ProCys: 0.578 ± 0.251
4.213ProAsp: 4.213 ± 0.691
5.121ProGlu: 5.121 ± 0.644
1.569ProPhe: 1.569 ± 0.381
5.039ProGly: 5.039 ± 0.925
0.496ProHis: 0.496 ± 0.197
1.982ProIle: 1.982 ± 0.441
1.569ProLys: 1.569 ± 0.282
5.287ProLeu: 5.287 ± 0.855
1.569ProMet: 1.569 ± 0.379
1.982ProAsn: 1.982 ± 0.382
2.396ProPro: 2.396 ± 0.697
2.313ProGln: 2.313 ± 0.488
3.056ProArg: 3.056 ± 0.472
3.635ProSer: 3.635 ± 0.598
3.882ProThr: 3.882 ± 0.679
5.204ProVal: 5.204 ± 0.865
0.991ProTrp: 0.991 ± 0.303
1.652ProTyr: 1.652 ± 0.317
0.0ProXaa: 0.0 ± 0.0
Gln
5.039GlnAla: 5.039 ± 0.68
0.33GlnCys: 0.33 ± 0.147
2.809GlnAsp: 2.809 ± 0.378
3.139GlnGlu: 3.139 ± 0.778
0.909GlnPhe: 0.909 ± 0.237
2.974GlnGly: 2.974 ± 0.508
0.248GlnHis: 0.248 ± 0.133
1.322GlnIle: 1.322 ± 0.412
0.826GlnLys: 0.826 ± 0.297
2.643GlnLeu: 2.643 ± 0.768
0.991GlnMet: 0.991 ± 0.281
0.165GlnAsn: 0.165 ± 0.134
2.396GlnPro: 2.396 ± 0.436
0.165GlnGln: 0.165 ± 0.106
3.139GlnArg: 3.139 ± 0.529
1.487GlnSer: 1.487 ± 0.23
1.817GlnThr: 1.817 ± 0.439
2.974GlnVal: 2.974 ± 0.431
0.578GlnTrp: 0.578 ± 0.276
0.413GlnTyr: 0.413 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
7.847ArgAla: 7.847 ± 1.234
0.826ArgCys: 0.826 ± 0.405
4.956ArgAsp: 4.956 ± 0.576
4.295ArgGlu: 4.295 ± 0.53
2.809ArgPhe: 2.809 ± 0.514
4.791ArgGly: 4.791 ± 0.727
1.074ArgHis: 1.074 ± 0.37
3.469ArgIle: 3.469 ± 0.603
2.974ArgLys: 2.974 ± 0.537
8.508ArgLeu: 8.508 ± 0.789
1.074ArgMet: 1.074 ± 0.292
2.065ArgAsn: 2.065 ± 0.46
3.882ArgPro: 3.882 ± 0.524
2.891ArgGln: 2.891 ± 0.565
7.021ArgArg: 7.021 ± 0.775
4.295ArgSer: 4.295 ± 0.581
3.882ArgThr: 3.882 ± 0.639
5.287ArgVal: 5.287 ± 0.691
1.652ArgTrp: 1.652 ± 0.376
2.396ArgTyr: 2.396 ± 0.521
0.0ArgXaa: 0.0 ± 0.0
Ser
7.187SerAla: 7.187 ± 0.876
0.165SerCys: 0.165 ± 0.135
4.378SerAsp: 4.378 ± 0.703
2.561SerGlu: 2.561 ± 0.494
2.478SerPhe: 2.478 ± 0.549
6.113SerGly: 6.113 ± 0.72
1.074SerHis: 1.074 ± 0.277
1.239SerIle: 1.239 ± 0.42
1.982SerLys: 1.982 ± 0.481
4.13SerLeu: 4.13 ± 0.77
1.322SerMet: 1.322 ± 0.281
1.156SerAsn: 1.156 ± 0.358
3.635SerPro: 3.635 ± 0.751
1.9SerGln: 1.9 ± 0.349
3.882SerArg: 3.882 ± 0.668
2.891SerSer: 2.891 ± 0.691
3.8SerThr: 3.8 ± 0.622
3.8SerVal: 3.8 ± 0.547
0.909SerTrp: 0.909 ± 0.404
1.156SerTyr: 1.156 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
8.839ThrAla: 8.839 ± 0.979
0.413ThrCys: 0.413 ± 0.173
4.13ThrAsp: 4.13 ± 0.675
3.965ThrGlu: 3.965 ± 0.595
2.561ThrPhe: 2.561 ± 0.373
5.452ThrGly: 5.452 ± 0.72
0.826ThrHis: 0.826 ± 0.255
2.313ThrIle: 2.313 ± 0.715
1.9ThrLys: 1.9 ± 0.493
5.617ThrLeu: 5.617 ± 0.781
1.404ThrMet: 1.404 ± 0.296
1.322ThrAsn: 1.322 ± 0.443
4.626ThrPro: 4.626 ± 0.598
1.817ThrGln: 1.817 ± 0.386
4.295ThrArg: 4.295 ± 0.497
3.552ThrSer: 3.552 ± 0.777
4.295ThrThr: 4.295 ± 0.685
5.7ThrVal: 5.7 ± 0.999
0.496ThrTrp: 0.496 ± 0.228
2.313ThrTyr: 2.313 ± 0.392
0.0ThrXaa: 0.0 ± 0.0
Val
7.765ValAla: 7.765 ± 0.852
0.496ValCys: 0.496 ± 0.228
4.791ValAsp: 4.791 ± 0.583
3.8ValGlu: 3.8 ± 0.534
2.148ValPhe: 2.148 ± 0.346
4.708ValGly: 4.708 ± 0.756
1.569ValHis: 1.569 ± 0.373
3.387ValIle: 3.387 ± 0.419
3.139ValLys: 3.139 ± 0.75
5.617ValLeu: 5.617 ± 0.779
1.404ValMet: 1.404 ± 0.359
2.726ValAsn: 2.726 ± 0.439
5.204ValPro: 5.204 ± 0.62
2.974ValGln: 2.974 ± 0.614
6.526ValArg: 6.526 ± 0.736
4.378ValSer: 4.378 ± 0.628
7.104ValThr: 7.104 ± 1.133
5.865ValVal: 5.865 ± 0.865
1.652ValTrp: 1.652 ± 0.382
2.065ValTyr: 2.065 ± 0.484
0.0ValXaa: 0.0 ± 0.0
Trp
2.974TrpAla: 2.974 ± 0.469
0.083TrpCys: 0.083 ± 0.081
0.743TrpAsp: 0.743 ± 0.211
1.322TrpGlu: 1.322 ± 0.332
0.826TrpPhe: 0.826 ± 0.285
1.322TrpGly: 1.322 ± 0.382
0.413TrpHis: 0.413 ± 0.208
0.413TrpIle: 0.413 ± 0.235
0.743TrpLys: 0.743 ± 0.217
1.074TrpLeu: 1.074 ± 0.313
0.248TrpMet: 0.248 ± 0.143
0.578TrpAsn: 0.578 ± 0.198
0.743TrpPro: 0.743 ± 0.243
0.496TrpGln: 0.496 ± 0.265
1.487TrpArg: 1.487 ± 0.331
1.652TrpSer: 1.652 ± 0.478
1.487TrpThr: 1.487 ± 0.559
1.487TrpVal: 1.487 ± 0.57
0.083TrpTrp: 0.083 ± 0.082
0.248TrpTyr: 0.248 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.552TyrAla: 3.552 ± 0.502
0.33TyrCys: 0.33 ± 0.146
1.817TyrAsp: 1.817 ± 0.377
0.743TyrGlu: 0.743 ± 0.225
0.496TyrPhe: 0.496 ± 0.215
2.478TyrGly: 2.478 ± 0.387
0.248TyrHis: 0.248 ± 0.135
0.413TyrIle: 0.413 ± 0.152
0.578TyrLys: 0.578 ± 0.178
2.561TyrLeu: 2.561 ± 0.347
0.33TyrMet: 0.33 ± 0.158
0.413TyrAsn: 0.413 ± 0.165
0.496TyrPro: 0.496 ± 0.201
0.496TyrGln: 0.496 ± 0.179
2.891TyrArg: 2.891 ± 0.495
1.239TyrSer: 1.239 ± 0.319
1.074TyrThr: 1.074 ± 0.356
2.726TyrVal: 2.726 ± 0.457
0.33TyrTrp: 0.33 ± 0.243
0.33TyrTyr: 0.33 ± 0.137
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (12107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski