Arthrobacter phage TripleJ

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triplejayvirus; Arthrobacter virus TripleJ

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TIP3|A0A5J6TIP3_9CAUD RecT-like ssDNA binding protein OS=Arthrobacter phage TripleJ OX=2599838 GN=55 PE=4 SV=1
MM1 pKa = 6.59TAEE4 pKa = 5.08EE5 pKa = 4.02IPEE8 pKa = 4.24RR9 pKa = 11.84EE10 pKa = 3.65WRR12 pKa = 11.84TAYY15 pKa = 9.47WGTCSDD21 pKa = 3.89QDD23 pKa = 4.2CLTSMVIYY31 pKa = 7.17PHH33 pKa = 7.55PDD35 pKa = 3.1RR36 pKa = 11.84ATDD39 pKa = 4.08LPDD42 pKa = 3.44EE43 pKa = 4.78EE44 pKa = 4.63EE45 pKa = 4.54WEE47 pKa = 4.4DD48 pKa = 3.31STFFIDD54 pKa = 6.05CPVCDD59 pKa = 5.34SSMDD63 pKa = 3.27WGGTDD68 pKa = 3.71HH69 pKa = 7.26PADD72 pKa = 4.34IIKK75 pKa = 10.4HH76 pKa = 4.53YY77 pKa = 11.0

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TFU6|A0A5J6TFU6_9CAUD SsDNA binding protein OS=Arthrobacter phage TripleJ OX=2599838 GN=56 PE=3 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84RR3 pKa = 11.84AWWKK7 pKa = 9.35PKK9 pKa = 9.87PPPPSQVEE17 pKa = 3.91ALHH20 pKa = 6.18ARR22 pKa = 11.84EE23 pKa = 3.74LATRR27 pKa = 11.84QLEE30 pKa = 4.61SANEE34 pKa = 3.82RR35 pKa = 11.84HH36 pKa = 6.23AEE38 pKa = 4.28AITLAEE44 pKa = 4.2TLRR47 pKa = 11.84QIRR50 pKa = 11.84QRR52 pKa = 11.84NHH54 pKa = 6.61FGQSLEE60 pKa = 3.84NLNWSKK66 pKa = 10.42QQ67 pKa = 3.43

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14517

42

1461

177.0

19.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.868 ± 0.691

0.827 ± 0.141

6.207 ± 0.242

5.862 ± 0.284

2.687 ± 0.188

8.287 ± 0.43

2.067 ± 0.161

4.409 ± 0.197

4.23 ± 0.228

7.97 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.115

3.217 ± 0.153

5.104 ± 0.413

4.154 ± 0.198

6.062 ± 0.267

5.538 ± 0.191

7.281 ± 0.294

6.785 ± 0.266

1.743 ± 0.162

2.459 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski