Yersinia phage Yep-phi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Berlinvirus; Yersinia virus Yepf

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5L7E1|E5L7E1_9CAUD Exonuclease OS=Yersinia phage Yep-phi OX=928293 GN=YEP_phi_CDS00025 PE=4 SV=1
MM1 pKa = 7.66SLLTDD6 pKa = 3.63NVQVTRR12 pKa = 11.84EE13 pKa = 4.21AWNDD17 pKa = 3.18MQVYY21 pKa = 9.22IQSLEE26 pKa = 3.88KK27 pKa = 10.82DD28 pKa = 3.65QEE30 pKa = 4.08FLDD33 pKa = 3.74ALRR36 pKa = 11.84ACGVDD41 pKa = 3.04NWDD44 pKa = 3.87GYY46 pKa = 11.34LDD48 pKa = 3.82AVEE51 pKa = 4.45MVCRR55 pKa = 11.84EE56 pKa = 4.11DD57 pKa = 5.05DD58 pKa = 3.57LL59 pKa = 6.7

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5L7C5|E5L7C5_9CAUD Gp1.7 OS=Yersinia phage Yep-phi OX=928293 GN=YEP_phi_CDS00009 PE=4 SV=1
MM1 pKa = 7.61LRR3 pKa = 11.84LHH5 pKa = 6.6FNKK8 pKa = 10.45SNGIYY13 pKa = 9.76SVRR16 pKa = 11.84NSGRR20 pKa = 11.84SLVKK24 pKa = 9.73ATEE27 pKa = 3.87KK28 pKa = 10.51HH29 pKa = 5.28AVIPKK34 pKa = 9.9VLGATIEE41 pKa = 3.98LAPRR45 pKa = 11.84VHH47 pKa = 7.02AVITTGLFKK56 pKa = 10.67EE57 pKa = 4.35ATTGSRR63 pKa = 11.84PFIPVLVTRR72 pKa = 11.84FPKK75 pKa = 9.72VRR77 pKa = 11.84LVIEE81 pKa = 4.63CIKK84 pKa = 10.8EE85 pKa = 4.08VFF87 pKa = 3.46

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12032

26

1315

267.4

29.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.718 ± 0.494

1.022 ± 0.184

6.333 ± 0.252

7.123 ± 0.32

3.507 ± 0.171

7.821 ± 0.319

1.828 ± 0.189

4.845 ± 0.192

6.898 ± 0.322

8.236 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.241 ± 0.174

4.729 ± 0.242

3.557 ± 0.198

3.782 ± 0.248

5.303 ± 0.192

6.092 ± 0.397

5.394 ± 0.22

6.774 ± 0.377

1.479 ± 0.184

3.316 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski