Phaeodactylibacter xiamenensis
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5064 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098S0C5|A0A098S0C5_9BACT Uncharacterized protein OS=Phaeodactylibacter xiamenensis OX=1524460 GN=IX84_26190 PE=4 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.45 KK3 pKa = 10.4 LYY5 pKa = 9.9 QLLAICCLVSLAANPLMAQIPNDD28 pKa = 3.5 SPIPVDD34 pKa = 3.58 VFFEE38 pKa = 4.51 VPGTGLTRR46 pKa = 11.84 TIGDD50 pKa = 3.67 YY51 pKa = 10.87 TSVSNSTEE59 pKa = 3.45 WGTQPLRR66 pKa = 11.84 TTVSAEE72 pKa = 4.05 VMWVDD77 pKa = 4.92 DD78 pKa = 5.18 DD79 pKa = 6.12 DD80 pKa = 4.26 IAGDD84 pKa = 3.8 GMDD87 pKa = 4.2 TLGCDD92 pKa = 3.04 TSTMVDD98 pKa = 3.57 YY99 pKa = 10.22 TDD101 pKa = 3.41 KK102 pKa = 10.66 MVLIRR107 pKa = 11.84 RR108 pKa = 11.84 GGCFFSDD115 pKa = 3.99 KK116 pKa = 10.36 IYY118 pKa = 10.59 YY119 pKa = 8.7 AQQAGAIAVVIVNADD134 pKa = 3.22 GSDD137 pKa = 3.38 ATGGMAAGDD146 pKa = 3.84 EE147 pKa = 4.44 KK148 pKa = 11.11 GLEE151 pKa = 3.98 VTIPSVFLNDD161 pKa = 3.72 PGEE164 pKa = 4.03 AAGFIEE170 pKa = 5.33 ALDD173 pKa = 3.94 AGEE176 pKa = 4.2 TVIGTFQVRR185 pKa = 11.84 GFFGDD190 pKa = 4.41 LGPDD194 pKa = 2.95 RR195 pKa = 11.84 YY196 pKa = 9.12 GTPLAQVTPFEE207 pKa = 4.95 NISVDD212 pKa = 4.59 LLNLDD217 pKa = 3.4 PMEE220 pKa = 4.66 PVLDD224 pKa = 3.83 VSASVDD230 pKa = 3.45 IIAPDD235 pKa = 3.57 GTEE238 pKa = 3.82 TKK240 pKa = 9.46 LTAEE244 pKa = 4.41 VDD246 pKa = 3.92 TINPDD251 pKa = 3.49 VIHH254 pKa = 6.04 TFEE257 pKa = 4.49 FEE259 pKa = 4.39 PYY261 pKa = 8.81 TPAEE265 pKa = 4.1 VGTYY269 pKa = 10.36 EE270 pKa = 3.96 MLYY273 pKa = 10.03 TNSLTADD280 pKa = 3.43 TLRR283 pKa = 11.84 RR284 pKa = 11.84 QFAVTDD290 pKa = 3.71 FTFQMDD296 pKa = 3.59 NGNIPEE302 pKa = 4.33 WPVDD306 pKa = 3.09 SWIATTSEE314 pKa = 4.15 GFVEE318 pKa = 5.78 DD319 pKa = 4.36 LLTYY323 pKa = 10.89 DD324 pKa = 3.95 FGNYY328 pKa = 9.36 FEE330 pKa = 5.65 TNDD333 pKa = 3.48 QGAMATHH340 pKa = 6.84 ATFSLGNPDD349 pKa = 4.58 SIRR352 pKa = 11.84 TGFNDD357 pKa = 3.34 ADD359 pKa = 3.59 IFFLTLFDD367 pKa = 4.53 TDD369 pKa = 4.2 PDD371 pKa = 4.15 GDD373 pKa = 4.15 GQSATGDD380 pKa = 3.7 EE381 pKa = 4.25 IDD383 pKa = 3.58 YY384 pKa = 9.18 STFEE388 pKa = 3.96 IVGQAVYY395 pKa = 10.36 ILNGNEE401 pKa = 3.87 EE402 pKa = 4.75 PYY404 pKa = 11.29 DD405 pKa = 3.82 LLTVEE410 pKa = 4.76 FPEE413 pKa = 4.48 PVEE416 pKa = 4.63 LKK418 pKa = 11.08 ANTGYY423 pKa = 11.56 LLMAQYY429 pKa = 11.11 NGVNAALGIPPWYY442 pKa = 9.18 TYY444 pKa = 11.03 AGNEE448 pKa = 4.21 AYY450 pKa = 9.45 PGLSTMTFTDD460 pKa = 3.28 QLYY463 pKa = 8.32 TGGWAGDD470 pKa = 3.39 FRR472 pKa = 11.84 AVVRR476 pKa = 11.84 LHH478 pKa = 6.75 LNGFSVDD485 pKa = 3.96 AEE487 pKa = 4.99 DD488 pKa = 5.15 ITLADD493 pKa = 3.36 NKK495 pKa = 10.22 ATVFPNPANEE505 pKa = 4.14 TFQLEE510 pKa = 4.36 LQLDD514 pKa = 4.65 EE515 pKa = 4.63 YY516 pKa = 11.34 ADD518 pKa = 3.94 EE519 pKa = 4.33 VRR521 pKa = 11.84 VALVDD526 pKa = 3.09 MFGRR530 pKa = 11.84 VIEE533 pKa = 4.28 RR534 pKa = 11.84 NVYY537 pKa = 9.69 EE538 pKa = 4.22 GVKK541 pKa = 10.51 GGTYY545 pKa = 9.58 TFNTANLAAGTYY557 pKa = 9.98 FLGIQTPEE565 pKa = 3.71 GQATKK570 pKa = 10.24 RR571 pKa = 11.84 IVVIHH576 pKa = 6.43
Molecular weight: 62.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.528
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.694
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 1.163
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A098S192|A0A098S192_9BACT N-acetylmuramic acid 6-phosphate etherase OS=Phaeodactylibacter xiamenensis OX=1524460 GN=murQ PE=3 SV=1
MM1 pKa = 7.51 AAITDD6 pKa = 3.76 RR7 pKa = 11.84 SITKK11 pKa = 9.91 VPMPGIGLKK20 pKa = 9.99 FRR22 pKa = 11.84 AIIKK26 pKa = 9.99 VDD28 pKa = 3.39 SLQAAVRR35 pKa = 11.84 NDD37 pKa = 2.66 IAEE40 pKa = 3.99 FGFRR44 pKa = 11.84 KK45 pKa = 9.77 RR46 pKa = 11.84 KK47 pKa = 10.05 VYY49 pKa = 9.89 AQCITSTAILRR60 pKa = 11.84 AKK62 pKa = 10.31 AIIWVVGHH70 pKa = 6.03 GPEE73 pKa = 4.0 VQARR77 pKa = 11.84 QLDD80 pKa = 3.51 AMLFYY85 pKa = 10.84 LAPGPFIIRR94 pKa = 11.84 LMNDD98 pKa = 3.24 MEE100 pKa = 4.61 PVRR103 pKa = 11.84 STRR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 NVNFAIWCKK117 pKa = 10.58 LCC119 pKa = 4.58
Molecular weight: 13.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.589
IPC_protein 10.16
Toseland 10.716
ProMoST 10.335
Dawson 10.789
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 11.082
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.716
DTASelect 10.467
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.833
IPC_peptide 10.921
IPC2_peptide 9.575
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5064
0
5064
1899700
38
5518
375.1
41.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.164 ± 0.038
1.013 ± 0.026
5.841 ± 0.036
6.768 ± 0.04
4.708 ± 0.024
7.534 ± 0.04
1.893 ± 0.021
5.848 ± 0.026
4.458 ± 0.05
9.926 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.122 ± 0.019
4.503 ± 0.034
4.622 ± 0.026
4.385 ± 0.025
4.893 ± 0.046
6.037 ± 0.035
5.702 ± 0.059
6.36 ± 0.031
1.354 ± 0.016
3.867 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here