Clostridium phage phiMMP01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Yongloolinvirus; Clostridioides virus phiMMP01

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8WFX1|A0A0A8WFX1_9CAUD Endolysin OS=Clostridium phage phiMMP01 OX=1582156 GN=PHIMMP01_20036 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.35KK3 pKa = 9.64QVYY6 pKa = 9.85YY7 pKa = 11.2NSLDD11 pKa = 3.64EE12 pKa = 4.21KK13 pKa = 11.01EE14 pKa = 4.82KK15 pKa = 10.68IISEE19 pKa = 4.05NSNLYY24 pKa = 10.24VIEE27 pKa = 4.48IYY29 pKa = 8.06EE30 pKa = 4.29TLNEE34 pKa = 4.09NYY36 pKa = 9.94LVLSSSPIEE45 pKa = 4.23DD46 pKa = 3.24EE47 pKa = 3.96KK48 pKa = 11.46LSYY51 pKa = 11.06EE52 pKa = 4.0EE53 pKa = 5.53LEE55 pKa = 4.25NEE57 pKa = 4.11LLIMTNEE64 pKa = 3.99LQGGLLL70 pKa = 3.62

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8WFY8|A0A0A8WFY8_9CAUD Uncharacterized protein OS=Clostridium phage phiMMP01 OX=1582156 GN=PHIMMP01_20073 PE=4 SV=1
MM1 pKa = 7.56NKK3 pKa = 10.07RR4 pKa = 11.84IICNWCGKK12 pKa = 9.91LFYY15 pKa = 10.12ISKK18 pKa = 9.34QSKK21 pKa = 10.11KK22 pKa = 9.82IYY24 pKa = 9.01CCKK27 pKa = 9.48RR28 pKa = 11.84CEE30 pKa = 4.06RR31 pKa = 11.84KK32 pKa = 9.87ANRR35 pKa = 11.84SNRR38 pKa = 11.84EE39 pKa = 3.63QQNN42 pKa = 3.03

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

12963

37

762

160.0

18.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.899 ± 0.577

1.119 ± 0.137

6.017 ± 0.207

8.563 ± 0.471

3.934 ± 0.197

5.361 ± 0.257

1.165 ± 0.136

8.887 ± 0.324

10.846 ± 0.512

8.493 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.122

7.305 ± 0.215

1.975 ± 0.168

3.009 ± 0.194

3.633 ± 0.138

6.21 ± 0.21

5.415 ± 0.291

5.67 ± 0.267

0.918 ± 0.087

4.397 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski