Malvastrum leaf curl virus - [G87]
Average proteome isoelectric point is 7.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2MDG7|Q2MDG7_9GEMI Transcriptional activator protein OS=Malvastrum leaf curl virus - [G87] OX=329291 GN=ac2 PE=3 SV=1
MM1 pKa = 7.71 HH2 pKa = 7.66 IYY4 pKa = 10.64 DD5 pKa = 3.93 CAIWGAPYY13 pKa = 10.35 KK14 pKa = 10.36 LPPSSPDD21 pKa = 3.46 CLDD24 pKa = 4.06 FEE26 pKa = 5.2 CPPIPKK32 pKa = 9.89 CLRR35 pKa = 11.84 TGRR38 pKa = 11.84 FSIKK42 pKa = 10.06 AKK44 pKa = 10.62 NYY46 pKa = 8.05 FLTYY50 pKa = 9.22 PEE52 pKa = 4.62 CSLTKK57 pKa = 10.64 EE58 pKa = 4.09 EE59 pKa = 5.75 ALSQLQNLQTPTNIKK74 pKa = 9.71 FIKK77 pKa = 9.57 ICRR80 pKa = 11.84 EE81 pKa = 3.54 LHH83 pKa = 6.27 NNGNPHH89 pKa = 6.59 LHH91 pKa = 6.66 VLMQFEE97 pKa = 4.6 GKK99 pKa = 10.13 YY100 pKa = 10.06 NCTNNRR106 pKa = 11.84 FFDD109 pKa = 4.02 LVSPTRR115 pKa = 11.84 STHH118 pKa = 4.78 FHH120 pKa = 6.87 PNIQGAKK127 pKa = 9.25 SSSDD131 pKa = 3.04 VKK133 pKa = 11.25 SYY135 pKa = 11.09 INKK138 pKa = 10.49 DD139 pKa = 2.62 GDD141 pKa = 3.98 TIEE144 pKa = 4.1 WGQFQVDD151 pKa = 3.41 GRR153 pKa = 11.84 SARR156 pKa = 11.84 GGQQSANDD164 pKa = 4.09 AYY166 pKa = 10.74 AQALNSGSKK175 pKa = 10.2 SEE177 pKa = 4.03 ALNVIRR183 pKa = 11.84 EE184 pKa = 4.21 LAPKK188 pKa = 10.52 DD189 pKa = 3.64 FVLQFHH195 pKa = 6.7 NLNANLDD202 pKa = 4.29 RR203 pKa = 11.84 IFTPPVEE210 pKa = 4.63 VYY212 pKa = 10.3 ISPFSSSSFDD222 pKa = 3.27 RR223 pKa = 11.84 VPEE226 pKa = 3.93 EE227 pKa = 4.04 LEE229 pKa = 3.6 EE230 pKa = 4.04 WAVDD234 pKa = 3.76 NVVSAAARR242 pKa = 11.84 PLRR245 pKa = 11.84 PMSIVVEE252 pKa = 4.27 GDD254 pKa = 2.97 SRR256 pKa = 11.84 TGKK259 pKa = 8.52 TMWARR264 pKa = 11.84 SLGPHH269 pKa = 6.71 NYY271 pKa = 10.18 LCGHH275 pKa = 7.37 LDD277 pKa = 4.41 LSPKK281 pKa = 10.0 IYY283 pKa = 11.0 SNDD286 pKa = 2.4 AWYY289 pKa = 10.92 NVIDD293 pKa = 5.2 DD294 pKa = 4.41 VDD296 pKa = 3.61 PHH298 pKa = 5.85 YY299 pKa = 11.0 LKK301 pKa = 10.7 HH302 pKa = 6.2 FKK304 pKa = 10.7 EE305 pKa = 4.46 FMGAQRR311 pKa = 11.84 DD312 pKa = 3.88 WPSNTKK318 pKa = 9.78 YY319 pKa = 10.76 GKK321 pKa = 9.21 PVQIKK326 pKa = 10.41 GGIPTIFLCNPGPNSSYY343 pKa = 11.07 KK344 pKa = 10.49 EE345 pKa = 3.93 YY346 pKa = 10.94 LDD348 pKa = 3.87 EE349 pKa = 4.78 EE350 pKa = 4.7 KK351 pKa = 11.14 NSALRR356 pKa = 11.84 NWALKK361 pKa = 9.88 NATFITLEE369 pKa = 3.98 EE370 pKa = 4.04 PLYY373 pKa = 10.77 SGSHH377 pKa = 5.18 QSPTPTRR384 pKa = 11.84 QEE386 pKa = 3.53 EE387 pKa = 4.21 ANQEE391 pKa = 4.15 EE392 pKa = 4.92 EE393 pKa = 4.45 GG394 pKa = 3.76
Molecular weight: 44.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.649
IPC2_protein 5.664
IPC_protein 5.703
Toseland 6.071
ProMoST 6.046
Dawson 5.957
Bjellqvist 5.97
Wikipedia 5.957
Rodwell 5.944
Grimsley 6.198
Solomon 5.957
Lehninger 5.944
Nozaki 6.224
DTASelect 6.402
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.326
Patrickios 3.897
IPC_peptide 5.97
IPC2_peptide 6.313
IPC2.peptide.svr19 6.278
Protein with the highest isoelectric point:
>tr|Q2MDH0|Q2MDH0_9GEMI Protein V2 OS=Malvastrum leaf curl virus - [G87] OX=329291 GN=av2 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.52 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.99 SPYY27 pKa = 10.45 ASRR30 pKa = 11.84 AAVPTVLVTNRR41 pKa = 11.84 KK42 pKa = 9.43 RR43 pKa = 11.84 SWTHH47 pKa = 5.91 RR48 pKa = 11.84 PMNRR52 pKa = 11.84 KK53 pKa = 8.16 PKK55 pKa = 8.24 MYY57 pKa = 10.47 RR58 pKa = 11.84 MYY60 pKa = 10.58 RR61 pKa = 11.84 SPDD64 pKa = 3.21 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 10.35 VQSFDD79 pKa = 3.26 AKK81 pKa = 11.06 NDD83 pKa = 3.27 IGHH86 pKa = 6.11 MGKK89 pKa = 10.22 VICLSDD95 pKa = 3.42 VTRR98 pKa = 11.84 GTGLTHH104 pKa = 7.09 RR105 pKa = 11.84 VGKK108 pKa = 9.78 RR109 pKa = 11.84 FCVKK113 pKa = 10.05 SLYY116 pKa = 10.31 FVGKK120 pKa = 9.14 IWMDD124 pKa = 3.34 EE125 pKa = 4.02 NIKK128 pKa = 10.41 VKK130 pKa = 10.66 NHH132 pKa = 5.45 TNTVLFWIVRR142 pKa = 11.84 DD143 pKa = 3.85 RR144 pKa = 11.84 RR145 pKa = 11.84 PTGTPNDD152 pKa = 3.71 FQQVFNVYY160 pKa = 10.67 DD161 pKa = 4.09 NEE163 pKa = 4.37 PSTATVKK170 pKa = 10.59 NDD172 pKa = 3.0 QRR174 pKa = 11.84 DD175 pKa = 3.36 RR176 pKa = 11.84 FQVLRR181 pKa = 11.84 RR182 pKa = 11.84 FQATVTGGQYY192 pKa = 10.18 AAKK195 pKa = 8.87 EE196 pKa = 3.58 QAIIRR201 pKa = 11.84 KK202 pKa = 8.9 FYY204 pKa = 10.66 RR205 pKa = 11.84 VNNSCSLQSPRR216 pKa = 11.84 SWEE219 pKa = 3.65 VRR221 pKa = 11.84 EE222 pKa = 3.97 SHH224 pKa = 6.52 RR225 pKa = 11.84 EE226 pKa = 3.91 CSSLVYY232 pKa = 10.63 GMHH235 pKa = 6.44 SCLKK239 pKa = 10.64 SRR241 pKa = 11.84 VCYY244 pKa = 10.44 FEE246 pKa = 5.03 SKK248 pKa = 10.68 EE249 pKa = 4.01 LFLL252 pKa = 6.48
Molecular weight: 29.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.502
IPC_protein 9.794
Toseland 10.335
ProMoST 9.984
Dawson 10.482
Bjellqvist 10.175
Wikipedia 10.657
Rodwell 10.847
Grimsley 10.54
Solomon 10.526
Lehninger 10.496
Nozaki 10.365
DTASelect 10.145
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.409
Patrickios 10.511
IPC_peptide 10.526
IPC2_peptide 9.194
IPC2.peptide.svr19 8.482
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1102
96
394
183.7
21.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.719 ± 0.702
2.904 ± 0.368
4.9 ± 0.345
4.9 ± 0.679
4.356 ± 0.529
4.537 ± 0.458
3.63 ± 0.524
5.082 ± 0.642
5.535 ± 0.624
7.35 ± 0.764
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.178 ± 0.528
5.717 ± 0.661
6.715 ± 0.731
4.628 ± 0.452
7.169 ± 1.276
9.074 ± 1.028
5.898 ± 0.916
5.808 ± 1.109
1.452 ± 0.175
3.448 ± 0.464
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here