Aspergillus foetidus dsRNA mycovirus
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I7HPJ2|I7HPJ2_9VIRU Isoform of I7I4A1 Uncharacterized protein OS=Aspergillus foetidus dsRNA mycovirus OX=1087068 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.42 LGVNVRR7 pKa = 11.84 AGVDD11 pKa = 2.92 IRR13 pKa = 11.84 RR14 pKa = 11.84 PPRR17 pKa = 11.84 PPEE20 pKa = 4.26 PGGHH24 pKa = 7.25 DD25 pKa = 3.24 LTMVIEE31 pKa = 4.58 SEE33 pKa = 4.23 FSRR36 pKa = 11.84 AARR39 pKa = 11.84 GFAYY43 pKa = 8.78 TVRR46 pKa = 11.84 FSRR49 pKa = 11.84 GFSDD53 pKa = 5.63 DD54 pKa = 2.75 AWLPKK59 pKa = 10.55 AEE61 pKa = 3.92 LRR63 pKa = 11.84 EE64 pKa = 4.11 YY65 pKa = 10.62 KK66 pKa = 8.92 QTLAGRR72 pKa = 11.84 DD73 pKa = 3.51 VKK75 pKa = 11.34 VDD77 pKa = 3.25 VCEE80 pKa = 4.48 GGFYY84 pKa = 10.64 LAAGKK89 pKa = 10.15 IMNLIHH95 pKa = 7.03 AFISDD100 pKa = 3.75 WEE102 pKa = 4.71 GGCIGEE108 pKa = 4.26 AQVDD112 pKa = 3.92 VSLPGPSDD120 pKa = 3.42 TPPITFRR127 pKa = 11.84 TQVSTRR133 pKa = 11.84 TVAPLKK139 pKa = 9.73 YY140 pKa = 9.76 SYY142 pKa = 11.32 VFAEE146 pKa = 3.83 RR147 pKa = 11.84 LIPEE151 pKa = 4.82 VIAEE155 pKa = 4.89 DD156 pKa = 3.98 DD157 pKa = 4.04 TPAPAALCAACDD169 pKa = 3.74 HH170 pKa = 6.84 EE171 pKa = 4.64 EE172 pKa = 4.77 AIATDD177 pKa = 4.85 LYY179 pKa = 11.25 VAGPVTDD186 pKa = 3.72 TLRR189 pKa = 11.84 VEE191 pKa = 4.11 WFVDD195 pKa = 3.39 VLEE198 pKa = 4.33 NAGLGSSFAAIGNFSHH214 pKa = 6.69 EE215 pKa = 4.22 ASEE218 pKa = 4.25 VLASYY223 pKa = 10.13 GCRR226 pKa = 11.84 RR227 pKa = 11.84 LPEE230 pKa = 4.3 WLWGEE235 pKa = 4.18 QFDD238 pKa = 4.9 PPPDD242 pKa = 3.26 IPRR245 pKa = 11.84 FILVGDD251 pKa = 4.28 RR252 pKa = 11.84 PRR254 pKa = 11.84 EE255 pKa = 3.9 GWLSTNRR262 pKa = 11.84 FLSILASRR270 pKa = 11.84 GYY272 pKa = 9.97 HH273 pKa = 4.83 AAAWAQHH280 pKa = 5.55 LKK282 pKa = 10.54 GLEE285 pKa = 3.97 GEE287 pKa = 4.92 FFFSCPPTGVYY298 pKa = 10.24 GGWLIEE304 pKa = 4.28 SAVDD308 pKa = 3.53 EE309 pKa = 5.06 RR310 pKa = 11.84 GPLCYY315 pKa = 10.41 GKK317 pKa = 9.62 IEE319 pKa = 4.44 CVAGLDD325 pKa = 3.59 PTFSLFCDD333 pKa = 3.12 RR334 pKa = 11.84 SFMGEE339 pKa = 3.88 RR340 pKa = 11.84 FPFRR344 pKa = 11.84 IAKK347 pKa = 9.52 EE348 pKa = 4.04 FGLSAVALTQGCTVTVPLDD367 pKa = 3.49 QTVSWLCVLAYY378 pKa = 9.99 AVGAWCVQDD387 pKa = 3.73 VRR389 pKa = 11.84 PMLDD393 pKa = 3.19 LEE395 pKa = 4.78 RR396 pKa = 11.84 YY397 pKa = 8.81 SSAVGIAQSLRR408 pKa = 11.84 RR409 pKa = 11.84 IVHH412 pKa = 5.37 PPGRR416 pKa = 11.84 FDD418 pKa = 3.12 IWDD421 pKa = 3.84 DD422 pKa = 3.64 SGAGMAFDD430 pKa = 5.72 NFFQLPPLLTDD441 pKa = 3.35 VLDD444 pKa = 3.82 RR445 pKa = 11.84 RR446 pKa = 11.84 LALLISYY453 pKa = 7.89 TRR455 pKa = 11.84 EE456 pKa = 3.57 GAPILWGEE464 pKa = 4.11 RR465 pKa = 11.84 WDD467 pKa = 3.99 PPGKK471 pKa = 9.66 RR472 pKa = 11.84 LYY474 pKa = 10.98 HH475 pKa = 6.33 MYY477 pKa = 10.87 DD478 pKa = 3.38 DD479 pKa = 4.79 LRR481 pKa = 11.84 EE482 pKa = 4.11 KK483 pKa = 10.84 VSPEE487 pKa = 3.79 EE488 pKa = 4.4 LDD490 pKa = 3.41 WEE492 pKa = 4.46 PGIVLEE498 pKa = 4.44 RR499 pKa = 11.84 RR500 pKa = 11.84 HH501 pKa = 6.59 LSVLCSARR509 pKa = 11.84 IFRR512 pKa = 11.84 LLAYY516 pKa = 9.97 LVRR519 pKa = 11.84 GYY521 pKa = 10.5 HH522 pKa = 6.19 ALRR525 pKa = 11.84 RR526 pKa = 11.84 PLSMRR531 pKa = 11.84 TVQLICWVCSRR542 pKa = 11.84 LDD544 pKa = 3.64 DD545 pKa = 4.4 PLTIVRR551 pKa = 11.84 FLWYY555 pKa = 9.19 PFSGLARR562 pKa = 11.84 HH563 pKa = 6.61 DD564 pKa = 4.19 LDD566 pKa = 4.25 FVLPLVEE573 pKa = 5.4 YY574 pKa = 10.29 APCWVMM580 pKa = 5.1
Molecular weight: 65.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.04
IPC2_protein 5.118
IPC_protein 5.092
Toseland 5.041
ProMoST 5.245
Dawson 5.092
Bjellqvist 5.219
Wikipedia 4.978
Rodwell 5.016
Grimsley 4.965
Solomon 5.092
Lehninger 5.054
Nozaki 5.219
DTASelect 5.397
Thurlkill 5.054
EMBOSS 5.041
Sillero 5.296
Patrickios 3.846
IPC_peptide 5.105
IPC2_peptide 5.296
IPC2.peptide.svr19 5.241
Protein with the highest isoelectric point:
>tr|I7I4A1|I7I4A1_9VIRU Uncharacterized protein OS=Aspergillus foetidus dsRNA mycovirus OX=1087068 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 SVQIVLEE9 pKa = 4.08 NARR12 pKa = 11.84 YY13 pKa = 8.67 AASLAEE19 pKa = 3.93 ARR21 pKa = 11.84 HH22 pKa = 5.11 YY23 pKa = 11.26 LGDD26 pKa = 3.66 EE27 pKa = 4.16 PFWWGSLAEE36 pKa = 4.28 GRR38 pKa = 11.84 HH39 pKa = 5.29 ALSHH43 pKa = 6.53 LSAGNDD49 pKa = 3.31 DD50 pKa = 3.9 TSRR53 pKa = 11.84 CLSKK57 pKa = 10.59 LWSPRR62 pKa = 11.84 SFWVPSFTPKK72 pKa = 10.41 GFPLICVSSAALASKK87 pKa = 10.3 YY88 pKa = 10.3 CSLLRR93 pKa = 11.84 SFDD96 pKa = 3.94 LDD98 pKa = 3.67 VEE100 pKa = 4.41 IASDD104 pKa = 4.14 LRR106 pKa = 11.84 ALAARR111 pKa = 11.84 LFRR114 pKa = 11.84 GICVYY119 pKa = 10.54 VVADD123 pKa = 3.91 TFSSPFYY130 pKa = 10.54 AFEE133 pKa = 5.07 SDD135 pKa = 3.92 APPEE139 pKa = 4.08 EE140 pKa = 4.23 LVSWLRR146 pKa = 11.84 NPYY149 pKa = 10.56 DD150 pKa = 3.83 LGCPHH155 pKa = 7.07 SDD157 pKa = 2.7 AYY159 pKa = 9.97 TKK161 pKa = 10.5 FRR163 pKa = 11.84 ASRR166 pKa = 11.84 IFRR169 pKa = 11.84 TEE171 pKa = 4.02 DD172 pKa = 2.71 VWGEE176 pKa = 3.74 LGAWPWGDD184 pKa = 3.49 LEE186 pKa = 4.3 KK187 pKa = 11.01 ALGEE191 pKa = 4.17 IEE193 pKa = 4.28 VKK195 pKa = 10.5 EE196 pKa = 3.89 RR197 pKa = 11.84 VIDD200 pKa = 3.86 PEE202 pKa = 3.54 LWARR206 pKa = 11.84 MWHH209 pKa = 6.0 EE210 pKa = 4.12 LDD212 pKa = 3.39 AGRR215 pKa = 11.84 ASRR218 pKa = 11.84 ALFWADD224 pKa = 3.27 SLWNRR229 pKa = 11.84 EE230 pKa = 3.21 RR231 pKa = 11.84 RR232 pKa = 11.84 FRR234 pKa = 11.84 QRR236 pKa = 11.84 HH237 pKa = 4.52 YY238 pKa = 11.37 FDD240 pKa = 4.75 LCSLYY245 pKa = 10.32 IVVHH249 pKa = 5.6 GVWSKK254 pKa = 11.35 EE255 pKa = 3.75 GAGDD259 pKa = 4.21 LFRR262 pKa = 11.84 LIAEE266 pKa = 4.44 QILHH270 pKa = 5.98 EE271 pKa = 4.2 MLVAGMSYY279 pKa = 9.24 EE280 pKa = 4.04 RR281 pKa = 11.84 MYY283 pKa = 8.58 FTCFWLWTTQYY294 pKa = 10.24 WSSLCQYY301 pKa = 9.22 VLKK304 pKa = 10.1 TRR306 pKa = 11.84 LLHH309 pKa = 5.78 ATAAQWTAICKK320 pKa = 7.39 EE321 pKa = 4.19 TTAVVTQTWMFPLTNRR337 pKa = 11.84 QHH339 pKa = 6.05 IASAYY344 pKa = 9.05 FLNAEE349 pKa = 4.48 DD350 pKa = 5.34 LNGFSDD356 pKa = 4.02 ADD358 pKa = 3.48 AMKK361 pKa = 11.08 GEE363 pKa = 4.31 VTVEE367 pKa = 3.81 VLKK370 pKa = 11.1 YY371 pKa = 10.44 ALNEE375 pKa = 4.01 FKK377 pKa = 10.25 YY378 pKa = 8.74 TYY380 pKa = 10.04 PSIEE384 pKa = 3.69 GRR386 pKa = 11.84 TFDD389 pKa = 5.51 AYY391 pKa = 10.96 LQEE394 pKa = 4.68 LLSAATSLLEE404 pKa = 4.77 PIYY407 pKa = 9.67 KK408 pKa = 9.82 TYY410 pKa = 11.09 ADD412 pKa = 4.32 KK413 pKa = 11.37 AKK415 pKa = 9.33 TWDD418 pKa = 3.44 EE419 pKa = 4.58 FISLRR424 pKa = 11.84 SAWAAGGVAGRR435 pKa = 11.84 RR436 pKa = 11.84 SRR438 pKa = 11.84 DD439 pKa = 3.27 VLGNQVSPPGAAKK452 pKa = 10.23 AYY454 pKa = 9.54 VMTMTEE460 pKa = 3.94 KK461 pKa = 10.55 QQWAMDD467 pKa = 3.38 WGKK470 pKa = 11.02 LYY472 pKa = 10.52 IEE474 pKa = 4.52 IAGKK478 pKa = 10.17 LDD480 pKa = 3.21 EE481 pKa = 5.11 RR482 pKa = 11.84 GVDD485 pKa = 3.31 RR486 pKa = 11.84 TLSATDD492 pKa = 3.61 MRR494 pKa = 11.84 DD495 pKa = 3.16 QTAEE499 pKa = 3.96 SYY501 pKa = 10.85 VFHH504 pKa = 7.59 PLRR507 pKa = 11.84 SRR509 pKa = 11.84 YY510 pKa = 8.9 PVVGLDD516 pKa = 3.12 IGEE519 pKa = 4.54 SPAEE523 pKa = 3.8 TMGRR527 pKa = 11.84 HH528 pKa = 5.56 INLIAASEE536 pKa = 4.75 GPAHH540 pKa = 6.13 YY541 pKa = 10.57 LSDD544 pKa = 3.82 GRR546 pKa = 11.84 ILVAWDD552 pKa = 3.01 WSKK555 pKa = 10.41 WDD557 pKa = 3.92 HH558 pKa = 5.6 YY559 pKa = 10.51 VHH561 pKa = 6.17 VAEE564 pKa = 5.12 HH565 pKa = 5.52 MVVLKK570 pKa = 10.94 AMRR573 pKa = 11.84 QLIRR577 pKa = 11.84 RR578 pKa = 11.84 YY579 pKa = 9.23 IRR581 pKa = 11.84 QEE583 pKa = 3.61 VATDD587 pKa = 3.13 MLRR590 pKa = 11.84 EE591 pKa = 3.72 IDD593 pKa = 4.32 RR594 pKa = 11.84 IEE596 pKa = 4.23 EE597 pKa = 4.05 GHH599 pKa = 6.12 RR600 pKa = 11.84 VAIFRR605 pKa = 11.84 CQAYY609 pKa = 10.4 ADD611 pKa = 3.76 DD612 pKa = 4.59 HH613 pKa = 6.4 YY614 pKa = 11.78 SRR616 pKa = 11.84 VVDD619 pKa = 4.86 EE620 pKa = 5.05 IIKK623 pKa = 10.53 GSGGRR628 pKa = 11.84 ARR630 pKa = 11.84 RR631 pKa = 11.84 VGDD634 pKa = 3.31 TAVEE638 pKa = 4.29 VTNPAGQQSGRR649 pKa = 11.84 RR650 pKa = 11.84 TTLEE654 pKa = 3.72 VNTIIGTSRR663 pKa = 11.84 LLVRR667 pKa = 11.84 DD668 pKa = 3.75 SEE670 pKa = 4.43 LLGRR674 pKa = 11.84 GASLRR679 pKa = 11.84 SRR681 pKa = 11.84 TSMYY685 pKa = 10.75 VLNRR689 pKa = 11.84 ADD691 pKa = 4.21 DD692 pKa = 3.66 VAEE695 pKa = 4.12 VFRR698 pKa = 11.84 KK699 pKa = 8.99 YY700 pKa = 10.31 QEE702 pKa = 3.64 GKK704 pKa = 10.15 AAVEE708 pKa = 4.07 KK709 pKa = 10.32 MLEE712 pKa = 4.11 QGHH715 pKa = 6.62 LANPKK720 pKa = 9.82 KK721 pKa = 10.29 QVAQWRR727 pKa = 11.84 SIVYY731 pKa = 8.65 FRR733 pKa = 11.84 ILYY736 pKa = 10.33 AGGAMRR742 pKa = 11.84 PFPPRR747 pKa = 11.84 AVYY750 pKa = 9.82 AAASGHH756 pKa = 5.61 PNKK759 pKa = 10.64 GSGGEE764 pKa = 3.8 LDD766 pKa = 5.23 AISKK770 pKa = 9.88 LRR772 pKa = 11.84 SISSGLEE779 pKa = 3.08 MWARR783 pKa = 11.84 RR784 pKa = 11.84 GGWLGMARR792 pKa = 11.84 ALYY795 pKa = 10.61 DD796 pKa = 3.41 DD797 pKa = 4.51 AEE799 pKa = 4.55 RR800 pKa = 11.84 FFSKK804 pKa = 9.44 TRR806 pKa = 11.84 VWTRR810 pKa = 11.84 ACHH813 pKa = 6.8 LNPKK817 pKa = 9.41 HH818 pKa = 6.32 PPDD821 pKa = 3.77 VVRR824 pKa = 11.84 LSPALLHH831 pKa = 6.53 AAPEE835 pKa = 4.14 NGGAGILPVGVYY847 pKa = 10.13 EE848 pKa = 3.64 YY849 pKa = 10.59 DD850 pKa = 3.52 YY851 pKa = 10.58 RR852 pKa = 11.84 IKK854 pKa = 10.72 AGRR857 pKa = 11.84 GKK859 pKa = 10.83 YY860 pKa = 9.68 EE861 pKa = 4.8 LDD863 pKa = 3.0 AQYY866 pKa = 10.02 WRR868 pKa = 11.84 KK869 pKa = 9.3 LADD872 pKa = 4.36 DD873 pKa = 3.88 YY874 pKa = 12.18 ANAHH878 pKa = 5.59 GRR880 pKa = 11.84 GPGLVDD886 pKa = 4.06 LEE888 pKa = 4.92 ANASSWVARR897 pKa = 11.84 ATGVTPTARR906 pKa = 11.84 DD907 pKa = 3.27 SLEE910 pKa = 4.08 FQRR913 pKa = 11.84 KK914 pKa = 4.09 WAAGRR919 pKa = 11.84 AQQDD923 pKa = 3.86 GEE925 pKa = 4.7 GIMITWLRR933 pKa = 11.84 NAAICDD939 pKa = 3.74 AVRR942 pKa = 11.84 TTHH945 pKa = 5.75 VDD947 pKa = 3.07 KK948 pKa = 11.26 SSSGHH953 pKa = 5.5 KK954 pKa = 10.03 FDD956 pKa = 3.66 WEE958 pKa = 4.27 SWSVRR963 pKa = 11.84 ACLLRR968 pKa = 11.84 FKK970 pKa = 10.64 QALQRR975 pKa = 11.84 ARR977 pKa = 11.84 RR978 pKa = 11.84 AIGAAEE984 pKa = 4.0 VTEE987 pKa = 4.62 TIRR990 pKa = 11.84 DD991 pKa = 3.34 IRR993 pKa = 11.84 GPPAYY998 pKa = 10.35 GMFKK1002 pKa = 9.77 SQYY1005 pKa = 8.69 YY1006 pKa = 9.63 GYY1008 pKa = 11.39 ASIIYY1013 pKa = 9.16 RR1014 pKa = 11.84 NSGKK1018 pKa = 10.35 DD1019 pKa = 3.23 PGVLRR1024 pKa = 11.84 SLAGGSALGRR1034 pKa = 11.84 DD1035 pKa = 4.26 YY1036 pKa = 11.12 LAQSGPFPDD1045 pKa = 3.71 WLKK1048 pKa = 11.21 VMHH1051 pKa = 6.95 LEE1053 pKa = 4.48 GKK1055 pKa = 10.2 LGAAGAWHH1063 pKa = 7.02 KK1064 pKa = 10.3 LLPPSWAGWLDD1075 pKa = 3.48 ATVSKK1080 pKa = 11.09 ALFEE1084 pKa = 4.39 TCLRR1088 pKa = 11.84 LPNVRR1093 pKa = 11.84 ASKK1096 pKa = 10.19 YY1097 pKa = 7.85 QVLMTRR1103 pKa = 11.84 SRR1105 pKa = 11.84 VTSFAVQMFRR1115 pKa = 11.84 HH1116 pKa = 6.33 AFPHH1120 pKa = 6.41 LFLHH1124 pKa = 7.11
Molecular weight: 126.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.264
IPC2_protein 8.317
IPC_protein 8.2
Toseland 8.419
ProMoST 8.668
Dawson 8.96
Bjellqvist 9.019
Wikipedia 9.107
Rodwell 9.033
Grimsley 8.799
Solomon 9.019
Lehninger 8.975
Nozaki 9.048
DTASelect 8.873
Thurlkill 8.902
EMBOSS 9.092
Sillero 9.136
Patrickios 4.457
IPC_peptide 9.004
IPC2_peptide 7.863
IPC2.peptide.svr19 7.783
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
1
4
3231
580
1124
807.8
89.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.585 ± 0.585
1.578 ± 0.392
6.252 ± 0.347
5.262 ± 0.48
4.024 ± 0.456
8.233 ± 0.424
2.228 ± 0.204
3.621 ± 0.408
2.29 ± 0.678
9.564 ± 0.382
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.383 ± 0.256
2.105 ± 0.286
5.664 ± 0.703
2.507 ± 0.195
8.511 ± 0.234
7.119 ± 0.625
4.302 ± 0.146
7.83 ± 0.648
2.693 ± 0.337
3.25 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here