Phalaenopsis equestris amalgavirus 1
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G9M863|A0A3G9M863_9VIRU ORF1+2p OS=Phalaenopsis equestris amalgavirus 1 OX=2069327 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.6 SGPSQRR7 pKa = 11.84 VVHH10 pKa = 6.16 FPGGRR15 pKa = 11.84 ADD17 pKa = 3.57 PTVEE21 pKa = 4.05 LVEE24 pKa = 4.27 SLARR28 pKa = 11.84 YY29 pKa = 8.91 ANEE32 pKa = 3.81 GFRR35 pKa = 11.84 VHH37 pKa = 5.8 TWTPGILARR46 pKa = 11.84 SFIPVKK52 pKa = 10.57 RR53 pKa = 11.84 FIDD56 pKa = 3.64 AVRR59 pKa = 11.84 VLNNEE64 pKa = 3.6 PDD66 pKa = 3.27 EE67 pKa = 4.47 VVFNRR72 pKa = 11.84 LLALGVRR79 pKa = 11.84 DD80 pKa = 4.1 GWWEE84 pKa = 3.97 TTNTCTVSQFYY95 pKa = 10.61 KK96 pKa = 9.81 FCNWLKK102 pKa = 10.34 SAEE105 pKa = 4.09 GTTRR109 pKa = 11.84 LNEE112 pKa = 3.65 IRR114 pKa = 11.84 KK115 pKa = 9.16 ARR117 pKa = 11.84 HH118 pKa = 5.69 LEE120 pKa = 4.02 KK121 pKa = 10.36 KK122 pKa = 9.42 AQPGQTLEE130 pKa = 4.18 DD131 pKa = 3.66 VSLVAALDD139 pKa = 3.71 EE140 pKa = 4.34 QVAEE144 pKa = 4.15 FQQRR148 pKa = 11.84 KK149 pKa = 9.34 KK150 pKa = 7.62 EE151 pKa = 4.04 TRR153 pKa = 11.84 LEE155 pKa = 4.16 FEE157 pKa = 4.73 SKK159 pKa = 10.8 LIDD162 pKa = 3.78 LRR164 pKa = 11.84 RR165 pKa = 11.84 QIALVQQEE173 pKa = 4.2 MSATMKK179 pKa = 10.88 AHH181 pKa = 7.26 DD182 pKa = 5.01 ADD184 pKa = 4.94 FSPASIYY191 pKa = 11.03 EE192 pKa = 4.01 PMDD195 pKa = 4.03 DD196 pKa = 4.82 LEE198 pKa = 5.15 FGEE201 pKa = 5.14 ACWNLYY207 pKa = 9.8 RR208 pKa = 11.84 AEE210 pKa = 4.2 CARR213 pKa = 11.84 LNQDD217 pKa = 4.0 EE218 pKa = 4.98 APLDD222 pKa = 4.4 DD223 pKa = 5.18 GLLEE227 pKa = 4.38 AVKK230 pKa = 9.06 LTHH233 pKa = 6.73 GNAALAMHH241 pKa = 7.49 KK242 pKa = 10.95 ANFLRR247 pKa = 11.84 VGFNRR252 pKa = 11.84 NNLKK256 pKa = 10.05 HH257 pKa = 6.11 WIEE260 pKa = 4.01 EE261 pKa = 4.41 KK262 pKa = 10.41 ILEE265 pKa = 4.7 LDD267 pKa = 3.63 TVGEE271 pKa = 4.13 ARR273 pKa = 11.84 RR274 pKa = 11.84 ATTFRR279 pKa = 11.84 SYY281 pKa = 10.48 MATTGGALADD291 pKa = 3.78 EE292 pKa = 4.95 VRR294 pKa = 11.84 AEE296 pKa = 4.12 TQTRR300 pKa = 11.84 VAAAGGTRR308 pKa = 11.84 RR309 pKa = 11.84 TLRR312 pKa = 11.84 RR313 pKa = 11.84 ARR315 pKa = 11.84 DD316 pKa = 3.41 QVDD319 pKa = 3.59 VEE321 pKa = 4.36 NVQPSRR327 pKa = 11.84 LRR329 pKa = 11.84 PRR331 pKa = 11.84 VTTRR335 pKa = 11.84 DD336 pKa = 3.35 TGVEE340 pKa = 3.88 TAGALVIRR348 pKa = 11.84 DD349 pKa = 3.87 RR350 pKa = 11.84 ASSSHH355 pKa = 5.53 TAPPLAEE362 pKa = 4.53 PEE364 pKa = 4.31 AVHH367 pKa = 6.53 PPPAAPGPGGEE378 pKa = 4.53 DD379 pKa = 3.49 PPLEE383 pKa = 4.19 EE384 pKa = 4.71
Molecular weight: 42.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.786
IPC2_protein 5.703
IPC_protein 5.715
Toseland 6.059
ProMoST 5.893
Dawson 5.906
Bjellqvist 5.944
Wikipedia 5.893
Rodwell 5.881
Grimsley 6.198
Solomon 5.906
Lehninger 5.893
Nozaki 6.148
DTASelect 6.326
Thurlkill 6.3
EMBOSS 6.262
Sillero 6.249
Patrickios 4.647
IPC_peptide 5.919
IPC2_peptide 6.249
IPC2.peptide.svr19 6.24
Protein with the highest isoelectric point:
>tr|A0A3G9M863|A0A3G9M863_9VIRU ORF1+2p OS=Phalaenopsis equestris amalgavirus 1 OX=2069327 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.6 SGPSQRR7 pKa = 11.84 VVHH10 pKa = 6.16 FPGGRR15 pKa = 11.84 ADD17 pKa = 3.57 PTVEE21 pKa = 4.05 LVEE24 pKa = 4.27 SLARR28 pKa = 11.84 YY29 pKa = 8.91 ANEE32 pKa = 3.81 GFRR35 pKa = 11.84 VHH37 pKa = 5.8 TWTPGILARR46 pKa = 11.84 SFIPVKK52 pKa = 10.57 RR53 pKa = 11.84 FIDD56 pKa = 3.64 AVRR59 pKa = 11.84 VLNNEE64 pKa = 3.6 PDD66 pKa = 3.27 EE67 pKa = 4.47 VVFNRR72 pKa = 11.84 LLALGVRR79 pKa = 11.84 DD80 pKa = 4.1 GWWEE84 pKa = 3.97 TTNTCTVSQFYY95 pKa = 10.61 KK96 pKa = 9.81 FCNWLKK102 pKa = 10.34 SAEE105 pKa = 4.09 GTTRR109 pKa = 11.84 LNEE112 pKa = 3.65 IRR114 pKa = 11.84 KK115 pKa = 9.16 ARR117 pKa = 11.84 HH118 pKa = 5.69 LEE120 pKa = 4.02 KK121 pKa = 10.36 KK122 pKa = 9.42 AQPGQTLEE130 pKa = 4.18 DD131 pKa = 3.66 VSLVAALDD139 pKa = 3.71 EE140 pKa = 4.34 QVAEE144 pKa = 4.15 FQQRR148 pKa = 11.84 KK149 pKa = 9.34 KK150 pKa = 7.62 EE151 pKa = 4.04 TRR153 pKa = 11.84 LEE155 pKa = 4.16 FEE157 pKa = 4.73 SKK159 pKa = 10.8 LIDD162 pKa = 3.78 LRR164 pKa = 11.84 RR165 pKa = 11.84 QIALVQQEE173 pKa = 4.2 MSATMKK179 pKa = 10.88 AHH181 pKa = 7.26 DD182 pKa = 5.01 ADD184 pKa = 4.94 FSPASIYY191 pKa = 11.03 EE192 pKa = 4.01 PMDD195 pKa = 4.03 DD196 pKa = 4.82 LEE198 pKa = 5.15 FGEE201 pKa = 5.14 ACWNLYY207 pKa = 9.8 RR208 pKa = 11.84 AEE210 pKa = 4.2 CARR213 pKa = 11.84 LNQDD217 pKa = 4.0 EE218 pKa = 4.98 APLDD222 pKa = 4.4 DD223 pKa = 5.18 GLLEE227 pKa = 4.38 AVKK230 pKa = 9.06 LTHH233 pKa = 6.73 GNAALAMHH241 pKa = 7.49 KK242 pKa = 10.95 ANFLRR247 pKa = 11.84 VGFNRR252 pKa = 11.84 NNLKK256 pKa = 10.05 HH257 pKa = 6.11 WIEE260 pKa = 4.01 EE261 pKa = 4.41 KK262 pKa = 10.41 ILEE265 pKa = 4.7 LDD267 pKa = 3.63 TVGEE271 pKa = 4.13 ARR273 pKa = 11.84 RR274 pKa = 11.84 ATTFVPTWQQRR285 pKa = 11.84 VEE287 pKa = 3.6 RR288 pKa = 11.84 WLMKK292 pKa = 10.54 FEE294 pKa = 4.6 LKK296 pKa = 10.64 LRR298 pKa = 11.84 LEE300 pKa = 4.48 LLQQVVLGVPCAEE313 pKa = 4.46 PGTRR317 pKa = 11.84 LTLRR321 pKa = 11.84 MSNLLDD327 pKa = 3.88 FGLVLRR333 pKa = 11.84 RR334 pKa = 11.84 EE335 pKa = 4.36 ILGSKK340 pKa = 9.69 QPAPLSSEE348 pKa = 3.66 IEE350 pKa = 4.14 RR351 pKa = 11.84 AVLIPPLLWQNQRR364 pKa = 11.84 LSILRR369 pKa = 11.84 PLRR372 pKa = 11.84 PDD374 pKa = 3.06 LGGRR378 pKa = 11.84 IPHH381 pKa = 5.7 SRR383 pKa = 11.84 SRR385 pKa = 11.84 YY386 pKa = 5.37 EE387 pKa = 3.63 AKK389 pKa = 9.73 VRR391 pKa = 11.84 KK392 pKa = 9.73 VIGGGEE398 pKa = 3.96 MRR400 pKa = 11.84 DD401 pKa = 3.0 WRR403 pKa = 11.84 AANAMYY409 pKa = 10.46 RR410 pKa = 11.84 GGGSFSDD417 pKa = 3.71 ALKK420 pKa = 10.95 LLIDD424 pKa = 4.15 ARR426 pKa = 11.84 EE427 pKa = 4.17 DD428 pKa = 3.36 APGAILSEE436 pKa = 4.03 KK437 pKa = 9.65 WKK439 pKa = 10.02 VDD441 pKa = 3.18 SARR444 pKa = 11.84 RR445 pKa = 11.84 YY446 pKa = 10.21 LLLPCGLPVPRR457 pKa = 11.84 GPEE460 pKa = 3.57 ATKK463 pKa = 10.21 MKK465 pKa = 10.54 NFNDD469 pKa = 3.98 DD470 pKa = 3.2 ATAGPALRR478 pKa = 11.84 AFGILRR484 pKa = 11.84 KK485 pKa = 9.96 SGLKK489 pKa = 9.53 TSLEE493 pKa = 3.93 EE494 pKa = 4.43 FAWNCLDD501 pKa = 3.2 AFARR505 pKa = 11.84 GGAAEE510 pKa = 4.4 DD511 pKa = 3.97 CLPFVAARR519 pKa = 11.84 VGYY522 pKa = 7.78 RR523 pKa = 11.84 TKK525 pKa = 11.17 LLTLSDD531 pKa = 3.67 AWSKK535 pKa = 11.14 INSCKK540 pKa = 10.1 PLGRR544 pKa = 11.84 CVMMLDD550 pKa = 3.2 AHH552 pKa = 5.87 EE553 pKa = 4.36 QAFSSPLYY561 pKa = 10.41 NVLSNLTHH569 pKa = 7.2 LSRR572 pKa = 11.84 FQRR575 pKa = 11.84 NSGFCNSIVRR585 pKa = 11.84 ASSDD589 pKa = 2.63 WAMLWKK595 pKa = 10.4 DD596 pKa = 3.41 VSAASCVVEE605 pKa = 4.82 LDD607 pKa = 2.93 WKK609 pKa = 10.89 KK610 pKa = 10.72 FDD612 pKa = 4.24 RR613 pKa = 11.84 EE614 pKa = 4.03 RR615 pKa = 11.84 PADD618 pKa = 4.95 DD619 pKa = 3.34 ISFMIDD625 pKa = 3.13 VIISCFEE632 pKa = 4.0 AKK634 pKa = 10.07 DD635 pKa = 3.78 DD636 pKa = 3.99 YY637 pKa = 11.47 EE638 pKa = 4.11 EE639 pKa = 4.6 RR640 pKa = 11.84 LLLGYY645 pKa = 10.55 RR646 pKa = 11.84 IMLNRR651 pKa = 11.84 ALIEE655 pKa = 4.2 RR656 pKa = 11.84 CFVTDD661 pKa = 3.67 DD662 pKa = 3.76 GGVFHH667 pKa = 7.72 IDD669 pKa = 2.91 GMVPSGSLWTGWLDD683 pKa = 3.31 TALNILYY690 pKa = 9.99 IGAALRR696 pKa = 11.84 HH697 pKa = 5.45 VLPDD701 pKa = 3.57 TSQAVAKK708 pKa = 10.53 CAGDD712 pKa = 4.04 DD713 pKa = 3.86 NLTLFYY719 pKa = 10.67 TDD721 pKa = 5.42 LPDD724 pKa = 5.2 ASLLNLKK731 pKa = 10.19 KK732 pKa = 10.71 YY733 pKa = 9.83 LNEE736 pKa = 3.57 WFRR739 pKa = 11.84 AGIEE743 pKa = 4.09 DD744 pKa = 3.91 EE745 pKa = 4.96 DD746 pKa = 5.19 FIIHH750 pKa = 6.73 RR751 pKa = 11.84 PPYY754 pKa = 10.23 HH755 pKa = 5.77 ITRR758 pKa = 11.84 FQATFPPGTDD768 pKa = 3.12 LSKK771 pKa = 10.61 GTSHH775 pKa = 7.51 LLDD778 pKa = 3.45 SAKK781 pKa = 9.85 WIQIHH786 pKa = 6.74 GIMNIDD792 pKa = 3.4 EE793 pKa = 4.53 AAGLSHH799 pKa = 6.66 RR800 pKa = 11.84 WKK802 pKa = 10.74 YY803 pKa = 11.22 SFAGKK808 pKa = 9.41 PKK810 pKa = 10.27 FLSCYY815 pKa = 8.77 WEE817 pKa = 4.29 EE818 pKa = 3.98 NGNPIRR824 pKa = 11.84 PAHH827 pKa = 6.0 VNLEE831 pKa = 4.01 KK832 pKa = 10.97 LLWPEE837 pKa = 5.32 GIHH840 pKa = 6.05 KK841 pKa = 9.4 TIDD844 pKa = 3.47 DD845 pKa = 3.85 YY846 pKa = 11.81 LAAVISMVVDD856 pKa = 3.37 NPFNHH861 pKa = 6.68 HH862 pKa = 5.94 NVNHH866 pKa = 5.55 MMHH869 pKa = 7.34 RR870 pKa = 11.84 FCIIQQVKK878 pKa = 8.77 RR879 pKa = 11.84 LSVAGIRR886 pKa = 11.84 DD887 pKa = 3.68 DD888 pKa = 5.43 HH889 pKa = 7.41 ILSLAHH895 pKa = 6.37 IRR897 pKa = 11.84 GKK899 pKa = 10.05 EE900 pKa = 3.95 GEE902 pKa = 4.4 MVPFPMVAEE911 pKa = 4.29 WRR913 pKa = 11.84 RR914 pKa = 11.84 TQGWVDD920 pKa = 3.95 MEE922 pKa = 4.57 SMPYY926 pKa = 8.98 LKK928 pKa = 10.29 KK929 pKa = 10.49 YY930 pKa = 10.58 ISDD933 pKa = 3.66 FRR935 pKa = 11.84 HH936 pKa = 5.63 FAAGVMSLYY945 pKa = 10.64 ARR947 pKa = 11.84 QPGGGIDD954 pKa = 3.0 AWRR957 pKa = 11.84 FMDD960 pKa = 4.28 MIRR963 pKa = 11.84 GNVLLGGEE971 pKa = 4.05 QFGNDD976 pKa = 2.81 FRR978 pKa = 11.84 DD979 pKa = 3.6 WIKK982 pKa = 10.53 FLQNNPLTSYY992 pKa = 9.4 LKK994 pKa = 9.71 PMRR997 pKa = 11.84 RR998 pKa = 11.84 FRR1000 pKa = 11.84 PQKK1003 pKa = 9.56 AARR1006 pKa = 11.84 EE1007 pKa = 4.17 STEE1010 pKa = 4.92 DD1011 pKa = 3.37 IISKK1015 pKa = 10.0 FKK1017 pKa = 11.08 SFSACCGIGDD1027 pKa = 4.01 GRR1029 pKa = 11.84 TPFASTEE1036 pKa = 4.4 SYY1038 pKa = 10.99 GSWIADD1044 pKa = 3.4 LVPRR1048 pKa = 11.84 MQHH1051 pKa = 5.1 GKK1053 pKa = 10.62 APLATMM1059 pKa = 5.09
Molecular weight: 120.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.982
IPC2_protein 7.395
IPC_protein 7.22
Toseland 7.0
ProMoST 7.819
Dawson 7.936
Bjellqvist 8.346
Wikipedia 7.834
Rodwell 7.951
Grimsley 7.088
Solomon 7.966
Lehninger 7.98
Nozaki 8.609
DTASelect 8.083
Thurlkill 8.141
EMBOSS 8.156
Sillero 8.463
Patrickios 4.622
IPC_peptide 7.966
IPC2_peptide 7.41
IPC2.peptide.svr19 7.328
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1443
384
1059
721.5
81.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.425 ± 0.911
1.594 ± 0.248
5.96 ± 0.22
6.861 ± 1.01
4.435 ± 0.354
6.445 ± 0.204
2.495 ± 0.068
4.435 ± 0.821
4.643 ± 0.447
10.395 ± 0.574
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.495 ± 0.418
3.95 ± 0.02
5.128 ± 0.269
3.119 ± 0.236
8.177 ± 0.537
5.475 ± 0.586
4.782 ± 1.008
5.891 ± 0.628
2.356 ± 0.356
1.94 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here