Streptococcus phage Javan394

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B1N6|A0A4D6B1N6_9CAUD Minor capsid protein OS=Streptococcus phage Javan394 OX=2548147 GN=Javan394_0010 PE=4 SV=1
MM1 pKa = 7.73EE2 pKa = 5.0EE3 pKa = 4.16LALSTVGYY11 pKa = 9.21FIVLAVLVIVLIIILASEE29 pKa = 4.54CPVNIDD35 pKa = 3.75IDD37 pKa = 3.8TSEE40 pKa = 5.08DD41 pKa = 3.49NEE43 pKa = 4.41QKK45 pKa = 8.24EE46 pKa = 4.67TPAYY50 pKa = 9.14LSRR53 pKa = 11.84YY54 pKa = 8.77GRR56 pKa = 11.84IIQMM60 pKa = 3.98

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B683|A0A4D6B683_9CAUD Uncharacterized protein OS=Streptococcus phage Javan394 OX=2548147 GN=Javan394_0027 PE=4 SV=1
MM1 pKa = 7.23SQGLSVKK8 pKa = 10.19VDD10 pKa = 3.27VDD12 pKa = 3.72LSGATRR18 pKa = 11.84KK19 pKa = 9.83VSPASVARR27 pKa = 11.84GRR29 pKa = 11.84VAMAGQMMMDD39 pKa = 4.03MDD41 pKa = 4.8RR42 pKa = 11.84YY43 pKa = 9.81IPMRR47 pKa = 11.84GGALRR52 pKa = 11.84ASGSMGGSGEE62 pKa = 4.34TINYY66 pKa = 5.34NTVYY70 pKa = 10.81ARR72 pKa = 11.84AHH74 pKa = 6.21FYY76 pKa = 8.37GTNGIVTFRR85 pKa = 11.84KK86 pKa = 8.41YY87 pKa = 6.39TTPGTGKK94 pKa = 10.35DD95 pKa = 2.86WLTPAKK101 pKa = 9.77EE102 pKa = 4.2ANLEE106 pKa = 4.02SWKK109 pKa = 10.66QKK111 pKa = 10.03ALKK114 pKa = 10.74GMGLL118 pKa = 3.5

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11427

48

1516

224.1

25.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.677 ± 1.041

0.516 ± 0.089

7.045 ± 0.43

6.66 ± 0.601

4.516 ± 0.212

6.161 ± 0.707

1.321 ± 0.151

7.692 ± 0.316

8.62 ± 0.527

7.99 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.265

6.423 ± 0.459

2.59 ± 0.186

4.166 ± 0.322

3.422 ± 0.281

6.642 ± 0.5

5.785 ± 0.396

6.152 ± 0.239

1.138 ± 0.2

3.956 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski