Amino acid dipepetide frequency for gamma proteobacterium SS-5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.627AlaAla: 11.627 ± 0.158
1.215AlaCys: 1.215 ± 0.039
6.308AlaAsp: 6.308 ± 0.102
8.486AlaGlu: 8.486 ± 0.115
3.291AlaPhe: 3.291 ± 0.063
7.821AlaGly: 7.821 ± 0.116
1.962AlaHis: 1.962 ± 0.046
4.995AlaIle: 4.995 ± 0.073
3.595AlaLys: 3.595 ± 0.069
13.015AlaLeu: 13.015 ± 0.152
2.774AlaMet: 2.774 ± 0.052
2.969AlaAsn: 2.969 ± 0.067
4.026AlaPro: 4.026 ± 0.089
5.589AlaGln: 5.589 ± 0.086
6.918AlaArg: 6.918 ± 0.095
5.534AlaSer: 5.534 ± 0.071
3.706AlaThr: 3.706 ± 0.076
6.028AlaVal: 6.028 ± 0.077
1.324AlaTrp: 1.324 ± 0.039
2.386AlaTyr: 2.386 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.001CysAla: 1.001 ± 0.036
0.159CysCys: 0.159 ± 0.013
0.545CysAsp: 0.545 ± 0.021
0.487CysGlu: 0.487 ± 0.022
0.375CysPhe: 0.375 ± 0.018
0.948CysGly: 0.948 ± 0.034
0.407CysHis: 0.407 ± 0.027
0.499CysIle: 0.499 ± 0.022
0.298CysLys: 0.298 ± 0.018
1.207CysLeu: 1.207 ± 0.037
0.2CysMet: 0.2 ± 0.013
0.32CysAsn: 0.32 ± 0.018
0.643CysPro: 0.643 ± 0.028
0.491CysGln: 0.491 ± 0.024
0.813CysArg: 0.813 ± 0.034
0.59CysSer: 0.59 ± 0.026
0.408CysThr: 0.408 ± 0.023
0.554CysVal: 0.554 ± 0.025
0.139CysTrp: 0.139 ± 0.011
0.271CysTyr: 0.271 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
5.351AspAla: 5.351 ± 0.089
0.666AspCys: 0.666 ± 0.027
2.859AspAsp: 2.859 ± 0.066
3.726AspGlu: 3.726 ± 0.072
2.179AspPhe: 2.179 ± 0.048
4.129AspGly: 4.129 ± 0.086
1.211AspHis: 1.211 ± 0.035
2.935AspIle: 2.935 ± 0.059
1.977AspLys: 1.977 ± 0.049
6.87AspLeu: 6.87 ± 0.078
1.308AspMet: 1.308 ± 0.038
1.657AspAsn: 1.657 ± 0.05
3.235AspPro: 3.235 ± 0.054
2.93AspGln: 2.93 ± 0.077
3.657AspArg: 3.657 ± 0.066
3.091AspSer: 3.091 ± 0.062
2.153AspThr: 2.153 ± 0.054
2.918AspVal: 2.918 ± 0.06
1.2AspTrp: 1.2 ± 0.035
1.953AspTyr: 1.953 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.767GluAla: 6.767 ± 0.106
0.43GluCys: 0.43 ± 0.022
3.138GluAsp: 3.138 ± 0.052
3.843GluGlu: 3.843 ± 0.085
2.048GluPhe: 2.048 ± 0.051
4.316GluGly: 4.316 ± 0.066
1.571GluHis: 1.571 ± 0.041
3.578GluIle: 3.578 ± 0.072
2.193GluLys: 2.193 ± 0.059
7.723GluLeu: 7.723 ± 0.097
1.622GluMet: 1.622 ± 0.035
1.572GluAsn: 1.572 ± 0.042
2.934GluPro: 2.934 ± 0.066
5.065GluGln: 5.065 ± 0.099
5.366GluArg: 5.366 ± 0.087
2.694GluSer: 2.694 ± 0.051
2.865GluThr: 2.865 ± 0.055
4.155GluVal: 4.155 ± 0.065
0.624GluTrp: 0.624 ± 0.028
1.384GluTyr: 1.384 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.612PheAla: 3.612 ± 0.069
0.426PheCys: 0.426 ± 0.021
2.467PheAsp: 2.467 ± 0.05
2.081PheGlu: 2.081 ± 0.047
1.379PhePhe: 1.379 ± 0.043
2.886PheGly: 2.886 ± 0.063
0.783PheHis: 0.783 ± 0.024
2.072PheIle: 2.072 ± 0.047
1.121PheLys: 1.121 ± 0.037
3.238PheLeu: 3.238 ± 0.059
0.742PheMet: 0.742 ± 0.028
1.224PheAsn: 1.224 ± 0.034
1.34PhePro: 1.34 ± 0.033
1.309PheGln: 1.309 ± 0.034
2.034PheArg: 2.034 ± 0.042
2.495PheSer: 2.495 ± 0.057
1.551PheThr: 1.551 ± 0.04
2.173PheVal: 2.173 ± 0.048
0.487PheTrp: 0.487 ± 0.024
1.046PheTyr: 1.046 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
6.166GlyAla: 6.166 ± 0.096
0.946GlyCys: 0.946 ± 0.034
3.996GlyAsp: 3.996 ± 0.075
4.759GlyGlu: 4.759 ± 0.071
3.151GlyPhe: 3.151 ± 0.066
5.734GlyGly: 5.734 ± 0.094
1.985GlyHis: 1.985 ± 0.047
4.138GlyIle: 4.138 ± 0.067
3.354GlyLys: 3.354 ± 0.065
10.397GlyLeu: 10.397 ± 0.139
2.259GlyMet: 2.259 ± 0.053
2.178GlyAsn: 2.178 ± 0.053
2.947GlyPro: 2.947 ± 0.067
4.539GlyGln: 4.539 ± 0.071
5.654GlyArg: 5.654 ± 0.083
4.344GlySer: 4.344 ± 0.064
2.991GlyThr: 2.991 ± 0.077
5.077GlyVal: 5.077 ± 0.081
1.178GlyTrp: 1.178 ± 0.035
2.48GlyTyr: 2.48 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.031HisAla: 2.031 ± 0.051
0.365HisCys: 0.365 ± 0.021
1.156HisAsp: 1.156 ± 0.041
1.218HisGlu: 1.218 ± 0.038
0.961HisPhe: 0.961 ± 0.031
1.8HisGly: 1.8 ± 0.045
0.64HisHis: 0.64 ± 0.025
1.08HisIle: 1.08 ± 0.034
0.753HisLys: 0.753 ± 0.027
2.781HisLeu: 2.781 ± 0.059
0.457HisMet: 0.457 ± 0.022
0.665HisAsn: 0.665 ± 0.027
1.521HisPro: 1.521 ± 0.042
1.199HisGln: 1.199 ± 0.036
1.658HisArg: 1.658 ± 0.04
1.411HisSer: 1.411 ± 0.036
0.773HisThr: 0.773 ± 0.027
1.016HisVal: 1.016 ± 0.033
0.524HisTrp: 0.524 ± 0.025
0.813HisTyr: 0.813 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.147IleAla: 5.147 ± 0.074
0.596IleCys: 0.596 ± 0.023
3.72IleAsp: 3.72 ± 0.063
3.839IleGlu: 3.839 ± 0.065
1.448IlePhe: 1.448 ± 0.038
4.416IleGly: 4.416 ± 0.081
1.194IleHis: 1.194 ± 0.033
2.505IleIle: 2.505 ± 0.063
2.033IleLys: 2.033 ± 0.051
4.82IleLeu: 4.82 ± 0.067
0.912IleMet: 0.912 ± 0.034
1.921IleAsn: 1.921 ± 0.045
2.48IlePro: 2.48 ± 0.053
2.275IleGln: 2.275 ± 0.054
3.524IleArg: 3.524 ± 0.056
3.296IleSer: 3.296 ± 0.057
2.248IleThr: 2.248 ± 0.054
2.485IleVal: 2.485 ± 0.07
0.529IleTrp: 0.529 ± 0.023
1.336IleTyr: 1.336 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
3.977LysAla: 3.977 ± 0.069
0.238LysCys: 0.238 ± 0.019
1.836LysAsp: 1.836 ± 0.054
1.941LysGlu: 1.941 ± 0.047
0.839LysPhe: 0.839 ± 0.033
2.797LysGly: 2.797 ± 0.05
0.758LysHis: 0.758 ± 0.03
1.713LysIle: 1.713 ± 0.043
1.597LysLys: 1.597 ± 0.054
3.442LysLeu: 3.442 ± 0.069
0.815LysMet: 0.815 ± 0.034
1.058LysAsn: 1.058 ± 0.038
2.069LysPro: 2.069 ± 0.052
1.964LysGln: 1.964 ± 0.052
2.582LysArg: 2.582 ± 0.058
1.762LysSer: 1.762 ± 0.045
1.814LysThr: 1.814 ± 0.046
2.166LysVal: 2.166 ± 0.05
0.32LysTrp: 0.32 ± 0.019
0.782LysTyr: 0.782 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
14.387LeuAla: 14.387 ± 0.181
1.222LeuCys: 1.222 ± 0.037
7.315LeuAsp: 7.315 ± 0.088
7.529LeuGlu: 7.529 ± 0.104
4.401LeuPhe: 4.401 ± 0.079
9.495LeuGly: 9.495 ± 0.11
2.696LeuHis: 2.696 ± 0.057
6.067LeuIle: 6.067 ± 0.085
4.197LeuLys: 4.197 ± 0.068
15.294LeuLeu: 15.294 ± 0.222
2.648LeuMet: 2.648 ± 0.057
3.482LeuAsn: 3.482 ± 0.061
5.984LeuPro: 5.984 ± 0.095
5.756LeuGln: 5.756 ± 0.104
8.535LeuArg: 8.535 ± 0.112
7.337LeuSer: 7.337 ± 0.091
4.881LeuThr: 4.881 ± 0.107
7.44LeuVal: 7.44 ± 0.102
1.296LeuTrp: 1.296 ± 0.043
2.594LeuTyr: 2.594 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.797MetAla: 2.797 ± 0.058
0.115MetCys: 0.115 ± 0.01
1.347MetAsp: 1.347 ± 0.038
1.322MetGlu: 1.322 ± 0.039
0.552MetPhe: 0.552 ± 0.022
1.799MetGly: 1.799 ± 0.042
0.485MetHis: 0.485 ± 0.021
1.114MetIle: 1.114 ± 0.035
0.94MetLys: 0.94 ± 0.03
2.477MetLeu: 2.477 ± 0.054
0.529MetMet: 0.529 ± 0.026
0.88MetAsn: 0.88 ± 0.03
1.391MetPro: 1.391 ± 0.034
1.402MetGln: 1.402 ± 0.037
1.592MetArg: 1.592 ± 0.041
1.39MetSer: 1.39 ± 0.039
1.27MetThr: 1.27 ± 0.038
1.378MetVal: 1.378 ± 0.043
0.132MetTrp: 0.132 ± 0.013
0.34MetTyr: 0.34 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.691AsnAla: 2.691 ± 0.057
0.317AsnCys: 0.317 ± 0.02
1.502AsnAsp: 1.502 ± 0.045
1.469AsnGlu: 1.469 ± 0.039
0.938AsnPhe: 0.938 ± 0.03
2.241AsnGly: 2.241 ± 0.056
0.685AsnHis: 0.685 ± 0.026
1.528AsnIle: 1.528 ± 0.044
0.996AsnLys: 0.996 ± 0.037
3.526AsnLeu: 3.526 ± 0.063
0.639AsnMet: 0.639 ± 0.027
0.965AsnAsn: 0.965 ± 0.037
2.276AsnPro: 2.276 ± 0.049
1.555AsnGln: 1.555 ± 0.046
2.298AsnArg: 2.298 ± 0.049
1.65AsnSer: 1.65 ± 0.046
1.213AsnThr: 1.213 ± 0.046
1.28AsnVal: 1.28 ± 0.04
0.393AsnTrp: 0.393 ± 0.018
0.817AsnTyr: 0.817 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
4.955ProAla: 4.955 ± 0.091
0.468ProCys: 0.468 ± 0.026
3.109ProAsp: 3.109 ± 0.058
4.249ProGlu: 4.249 ± 0.072
1.717ProPhe: 1.717 ± 0.047
4.209ProGly: 4.209 ± 0.079
0.937ProHis: 0.937 ± 0.036
2.232ProIle: 2.232 ± 0.043
1.618ProLys: 1.618 ± 0.042
6.227ProLeu: 6.227 ± 0.105
1.2ProMet: 1.2 ± 0.031
1.296ProAsn: 1.296 ± 0.037
2.517ProPro: 2.517 ± 0.061
2.652ProGln: 2.652 ± 0.055
2.706ProArg: 2.706 ± 0.055
2.711ProSer: 2.711 ± 0.059
1.919ProThr: 1.919 ± 0.051
3.318ProVal: 3.318 ± 0.057
0.723ProTrp: 0.723 ± 0.031
1.239ProTyr: 1.239 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
7.685GlnAla: 7.685 ± 0.126
0.398GlnCys: 0.398 ± 0.02
2.285GlnAsp: 2.285 ± 0.049
2.832GlnGlu: 2.832 ± 0.055
1.33GlnPhe: 1.33 ± 0.039
5.163GlnGly: 5.163 ± 0.081
1.157GlnHis: 1.157 ± 0.037
2.789GlnIle: 2.789 ± 0.069
1.347GlnLys: 1.347 ± 0.037
6.535GlnLeu: 6.535 ± 0.123
1.294GlnMet: 1.294 ± 0.04
1.02GlnAsn: 1.02 ± 0.035
2.778GlnPro: 2.778 ± 0.056
3.81GlnGln: 3.81 ± 0.09
5.061GlnArg: 5.061 ± 0.087
2.196GlnSer: 2.196 ± 0.049
2.174GlnThr: 2.174 ± 0.043
3.887GlnVal: 3.887 ± 0.094
0.602GlnTrp: 0.602 ± 0.024
0.895GlnTyr: 0.895 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
5.893ArgAla: 5.893 ± 0.085
0.703ArgCys: 0.703 ± 0.029
3.699ArgAsp: 3.699 ± 0.06
4.204ArgGlu: 4.204 ± 0.074
3.018ArgPhe: 3.018 ± 0.059
4.245ArgGly: 4.245 ± 0.067
1.967ArgHis: 1.967 ± 0.046
4.039ArgIle: 4.039 ± 0.07
2.325ArgLys: 2.325 ± 0.057
10.354ArgLeu: 10.354 ± 0.153
1.68ArgMet: 1.68 ± 0.044
1.974ArgAsn: 1.974 ± 0.05
3.748ArgPro: 3.748 ± 0.066
4.832ArgGln: 4.832 ± 0.082
5.017ArgArg: 5.017 ± 0.087
3.424ArgSer: 3.424 ± 0.059
2.418ArgThr: 2.418 ± 0.051
4.467ArgVal: 4.467 ± 0.08
1.076ArgTrp: 1.076 ± 0.037
2.061ArgTyr: 2.061 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
5.803SerAla: 5.803 ± 0.078
0.605SerCys: 0.605 ± 0.023
2.816SerAsp: 2.816 ± 0.065
3.127SerGlu: 3.127 ± 0.066
1.917SerPhe: 1.917 ± 0.043
5.348SerGly: 5.348 ± 0.078
1.312SerHis: 1.312 ± 0.036
2.721SerIle: 2.721 ± 0.054
1.764SerLys: 1.764 ± 0.049
7.114SerLeu: 7.114 ± 0.09
1.207SerMet: 1.207 ± 0.035
1.592SerAsn: 1.592 ± 0.042
2.764SerPro: 2.764 ± 0.061
2.685SerGln: 2.685 ± 0.05
3.661SerArg: 3.661 ± 0.064
3.076SerSer: 3.076 ± 0.072
2.25SerThr: 2.25 ± 0.056
3.085SerVal: 3.085 ± 0.056
0.713SerTrp: 0.713 ± 0.028
1.491SerTyr: 1.491 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.089ThrAla: 4.089 ± 0.083
0.377ThrCys: 0.377 ± 0.019
2.444ThrAsp: 2.444 ± 0.083
2.657ThrGlu: 2.657 ± 0.068
1.207ThrPhe: 1.207 ± 0.037
3.994ThrGly: 3.994 ± 0.073
0.889ThrHis: 0.889 ± 0.032
1.706ThrIle: 1.706 ± 0.048
1.103ThrLys: 1.103 ± 0.041
5.245ThrLeu: 5.245 ± 0.079
0.656ThrMet: 0.656 ± 0.026
1.092ThrAsn: 1.092 ± 0.039
2.707ThrPro: 2.707 ± 0.056
2.212ThrGln: 2.212 ± 0.044
2.565ThrArg: 2.565 ± 0.047
2.169ThrSer: 2.169 ± 0.057
1.939ThrThr: 1.939 ± 0.092
2.197ThrVal: 2.197 ± 0.064
0.452ThrTrp: 0.452 ± 0.023
0.93ThrTyr: 0.93 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
6.028ValAla: 6.028 ± 0.089
0.638ValCys: 0.638 ± 0.029
3.54ValAsp: 3.54 ± 0.062
4.329ValGlu: 4.329 ± 0.061
2.26ValPhe: 2.26 ± 0.048
4.218ValGly: 4.218 ± 0.077
1.244ValHis: 1.244 ± 0.037
3.367ValIle: 3.367 ± 0.058
2.18ValLys: 2.18 ± 0.052
6.958ValLeu: 6.958 ± 0.103
1.554ValMet: 1.554 ± 0.04
1.935ValAsn: 1.935 ± 0.049
2.583ValPro: 2.583 ± 0.046
2.547ValGln: 2.547 ± 0.051
4.133ValArg: 4.133 ± 0.071
3.636ValSer: 3.636 ± 0.065
2.701ValThr: 2.701 ± 0.066
4.294ValVal: 4.294 ± 0.086
0.681ValTrp: 0.681 ± 0.026
1.426ValTyr: 1.426 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.04TrpAla: 1.04 ± 0.035
0.14TrpCys: 0.14 ± 0.012
0.627TrpAsp: 0.627 ± 0.026
0.552TrpGlu: 0.552 ± 0.023
0.471TrpPhe: 0.471 ± 0.021
0.841TrpGly: 0.841 ± 0.031
0.316TrpHis: 0.316 ± 0.018
0.626TrpIle: 0.626 ± 0.025
0.364TrpLys: 0.364 ± 0.02
2.027TrpLeu: 2.027 ± 0.054
0.31TrpMet: 0.31 ± 0.018
0.39TrpAsn: 0.39 ± 0.024
0.648TrpPro: 0.648 ± 0.029
0.985TrpGln: 0.985 ± 0.036
1.128TrpArg: 1.128 ± 0.036
0.709TrpSer: 0.709 ± 0.025
0.431TrpThr: 0.431 ± 0.022
0.871TrpVal: 0.871 ± 0.029
0.191TrpTrp: 0.191 ± 0.016
0.317TrpTyr: 0.317 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.523TyrAla: 2.523 ± 0.058
0.33TyrCys: 0.33 ± 0.019
1.296TyrAsp: 1.296 ± 0.04
1.238TyrGlu: 1.238 ± 0.032
0.987TyrPhe: 0.987 ± 0.029
1.874TyrGly: 1.874 ± 0.044
0.623TyrHis: 0.623 ± 0.027
1.017TyrIle: 1.017 ± 0.031
0.717TyrLys: 0.717 ± 0.028
3.306TyrLeu: 3.306 ± 0.057
0.442TyrMet: 0.442 ± 0.022
0.79TyrAsn: 0.79 ± 0.029
1.413TyrPro: 1.413 ± 0.039
1.441TyrGln: 1.441 ± 0.036
2.241TyrArg: 2.241 ± 0.05
1.534TyrSer: 1.534 ± 0.037
0.926TyrThr: 0.926 ± 0.032
1.461TyrVal: 1.461 ± 0.046
0.4TyrTrp: 0.4 ± 0.02
0.783TyrTyr: 0.783 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3199 proteins (1044158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski