Lyngbya sp. (strain PCC 8106) (Lyngbya aestuarii (strain CCY9616))
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6110 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0YVH9|A0YVH9_LYNSP Uncharacterized protein OS=Lyngbya sp. (strain PCC 8106) OX=313612 GN=L8106_21694 PE=4 SV=1
MM1 pKa = 7.48 TIDD4 pKa = 3.53 NQVHH8 pKa = 5.32 VKK10 pKa = 10.89 AEE12 pKa = 4.09 TRR14 pKa = 11.84 NHH16 pKa = 6.29 KK17 pKa = 10.69 GFFVQQAAYY26 pKa = 8.72 QLKK29 pKa = 10.66 SIDD32 pKa = 3.62 DD33 pKa = 4.06 SGWALICLDD42 pKa = 4.79 DD43 pKa = 4.22 AVCYY47 pKa = 10.59 YY48 pKa = 10.94 VDD50 pKa = 4.64 PDD52 pKa = 3.76 NLEE55 pKa = 4.29 ADD57 pKa = 3.72 SDD59 pKa = 4.13 DD60 pKa = 4.44 DD61 pKa = 4.68 SEE63 pKa = 4.44 EE64 pKa = 4.44 SEE66 pKa = 4.44 SS67 pKa = 3.65
Molecular weight: 7.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0YYV2|A0YYV2_LYNSP Uncharacterized protein OS=Lyngbya sp. (strain PCC 8106) OX=313612 GN=L8106_25942 PE=4 SV=1
MM1 pKa = 7.42 AQQTLKK7 pKa = 9.73 GTNRR11 pKa = 11.84 KK12 pKa = 8.73 KK13 pKa = 10.69 KK14 pKa = 8.48 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 VRR22 pKa = 11.84 MRR24 pKa = 11.84 TKK26 pKa = 10.19 NGQSVIRR33 pKa = 11.84 SRR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.16 KK38 pKa = 9.51 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSVV45 pKa = 3.12
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6110
0
6110
1960410
20
3820
320.9
35.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.057 ± 0.036
1.01 ± 0.013
5.101 ± 0.043
6.766 ± 0.033
4.079 ± 0.024
6.553 ± 0.047
1.794 ± 0.017
6.917 ± 0.03
4.89 ± 0.038
10.756 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.781 ± 0.015
4.661 ± 0.04
4.676 ± 0.031
5.717 ± 0.031
4.847 ± 0.034
6.731 ± 0.026
5.81 ± 0.038
6.382 ± 0.023
1.429 ± 0.014
3.044 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here