Cellulophaga phage phi4:1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lightbulbvirus; Cellulophaga virus Cba41

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A244|S0A244_9CAUD N-acetylmuramoyl-L-alanine amidase OS=Cellulophaga phage phi4:1 OX=1328029 GN=Phi4:1_gp058 PE=4 SV=1
MM1 pKa = 7.69SNTDD5 pKa = 2.78NCKK8 pKa = 8.4QTNIPEE14 pKa = 4.33TDD16 pKa = 3.29NTALEE21 pKa = 4.49CPIFLQDD28 pKa = 2.8TCVIVKK34 pKa = 10.28DD35 pKa = 3.89GQPFILSDD43 pKa = 3.83SNISLTEE50 pKa = 3.93YY51 pKa = 10.7NEE53 pKa = 3.8KK54 pKa = 10.66LIEE57 pKa = 4.16KK58 pKa = 9.99LVEE61 pKa = 4.16FNQKK65 pKa = 7.91ITLLEE70 pKa = 4.1NSNPEE75 pKa = 3.92LMDD78 pKa = 3.74FSFSIQEE85 pKa = 3.83TTLALLVSDD94 pKa = 5.34NIVASIDD101 pKa = 3.73LQSLITGGVTEE112 pKa = 4.35AKK114 pKa = 9.89EE115 pKa = 3.81VFIAVGGEE123 pKa = 4.1TTLTLSNNALSTTVNQVSIEE143 pKa = 4.32GIVQLEE149 pKa = 4.45GSSNDD154 pKa = 3.28YY155 pKa = 11.26SLTNNIVYY163 pKa = 10.16FSDD166 pKa = 3.52PLEE169 pKa = 4.51SGEE172 pKa = 4.07IVQVIYY178 pKa = 10.65KK179 pKa = 9.51YY180 pKa = 11.11

Molecular weight: 19.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A537|S0A537_9CAUD Uncharacterized protein OS=Cellulophaga phage phi4:1 OX=1328029 GN=Phi4:1_gp114 PE=4 SV=1
MM1 pKa = 7.18SQQFQKK7 pKa = 10.24EE8 pKa = 4.31QKK10 pKa = 8.29QHH12 pKa = 6.29SNGSLYY18 pKa = 10.4RR19 pKa = 11.84CRR21 pKa = 11.84EE22 pKa = 3.64SDD24 pKa = 3.1ISDD27 pKa = 3.35TLVYY31 pKa = 10.94DD32 pKa = 3.82RR33 pKa = 11.84FQIVKK38 pKa = 7.86VTPKK42 pKa = 10.64GYY44 pKa = 8.75TIKK47 pKa = 10.03IWSTTTRR54 pKa = 11.84WVSSSSKK61 pKa = 10.45KK62 pKa = 9.96RR63 pKa = 11.84FAYY66 pKa = 7.76KK67 pKa = 9.88TKK69 pKa = 10.65EE70 pKa = 3.63EE71 pKa = 3.97ALEE74 pKa = 3.97GFILRR79 pKa = 11.84KK80 pKa = 9.35RR81 pKa = 11.84RR82 pKa = 11.84QIKK85 pKa = 9.74ILQAQLSKK93 pKa = 11.12AKK95 pKa = 10.01RR96 pKa = 11.84FLIIAEE102 pKa = 4.28KK103 pKa = 10.72

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

197

0

197

45394

33

2289

230.4

26.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.043 ± 0.178

1.038 ± 0.086

6.129 ± 0.138

8.195 ± 0.276

4.536 ± 0.139

6.003 ± 0.204

1.412 ± 0.109

7.327 ± 0.16

9.169 ± 0.355

8.274 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.967 ± 0.102

6.576 ± 0.216

2.917 ± 0.1

3.071 ± 0.154

3.516 ± 0.122

6.915 ± 0.224

6.518 ± 0.27

5.783 ± 0.155

1.119 ± 0.08

4.494 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski